Browsing by Author "Uslu, Baris Atalay"

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Assessment of the Histopathological Changes Occurring in the Testis of the Mice Suffering From Experimental Diabetes Induced Using Alloxan
(Medwell online, 2009) Uslu, Baris Atalay; Ilhan, Fatma; Gulyuzf, Fetih; Karaca, Mehmet; Oner, Ahmet Cihat
In this study, we aimed to examine the effect of volatile oil extracts of Helichrysum plicatum DC (HP) and Tanacetum balsamita L. (TB) on the histopathology of the testicles of the mice that had alloxan-induced type 1 diabetes or with imitated physiopathology of type 2 diabetes. The volatile oil extracts of HP and TB; were injected intra. peritoneally in diabetic mice at 12.5, 25 and 50 mg kg(-1) doses. Control and reference groups received 0.2 mL serum physiologic intra peritoneally and oral glibenclamide at 0.3 mL kg(-1) dose, respectively. Blood samples were withdrawn from tail veins of the mice 1, 2 and 24 It after the treatment and the blood glucose levels were measured. We investigated testicular histopathology of the mice in control and diabetic groups. The examination of the testicles of diabetic mice revealed reduction in diameters of the seminiferous tubules and thickening in the wall of the seminiferous tubules in addition to degenerative changes and decline in the number of spermatogenic cells. There were multinucleated giant cells in the lumens of some seminiferous tubules. No significant shown was detected in the severity of lesions between control group of diabetic mice receiving serum physiologic and the diabetic mice treated with the extracts of H? and TB. TUNEL-positive cells were higher diabetic mice than in control mice. We monitored no marked encouraging effect of HP and TB volatile oil extracts on the testicular histopathology. The reason for this failure might be due to short term use of these extracts. Long term applications of volatile oil extracts of HP and TB at various doses remained to be done to elucidate the potential anti-diabetic effect of these extracts.
Concentrations of Nefa, Β-Hba, Triglycerides, and Certain Blood Metabolites in Healthy Colored Angora Goats During the Peripartum Period
(Tubitak Scientific & Technological Research Council Turkey, 2015) Eski, Funda; Tasal, Ibrahim; Karsli, Mehmet Akif; Sendag, Sait; Uslu, Baris Atalay; Wagner, Henrik; Wehrend, Axel
The aim of this study was to determine the changes in serum nonesterified fatty acid (NEFA), serum beta-hydroxybutyric acid (beta-HBA), triglycerides, Ca, Na, and other metabolites (bilirubin, glutamate dehydrogenase (GLDH)) in the blood of grazing, healthy goats at the time of parturition. Blood samples were taken weekly from the jugular vein of 11 goats, starting at week 2 antepartum (ap) until week 9 postpartum (pp). NEFA and beta-HBA concentrations increased from week 2 ap to 2 weeks pp. The increase in NEFA level was not significant; however, the beta-HBA levels were higher (P < 0.05) 2 weeks pp compared to the levels at 2 weeks ap. Triglycerides were recorded at maximum levels (P < 0.05) 2 weeks ap, with the lowest concentrations at 3 weeks pp. Bilirubin levels consistently increased up to 7 weeks pp, followed by a decrease. However, these changes were not significant. Similarly, GLDH activities increased until week 8 pp. A significant difference (P < 0.05) was recorded between the 1st week and 8th week pp. Ca and Na levels were lower during the 1st week pp and increased at 3 weeks pp. The results show that there are characteristic alterations of some metabolic blood parameters in goats around the time of parturition, which may be related to physiological changes.
Distribution of Eosinophil Granulocytes and Mast Cells in the Reproductive Tract of Female Goats in the Preimplantation Phase
(Springer, 2009) Karaca, Turan; Yoruk, Mecit; Uslu, Sema; Cetin, Yunus; Uslu, Baris Atalay
Changes in eosinophil granulocytes and mast cells post-insemination may affect conceptus implantation, but information regarding the numbers of such cells in the mammalian reproductive tract is limited. This study investigated the preimplantation distribution of eosinophil granulocytes and mast cells (MCs) in the reproductive tract organs of female goats. Uterus, uterine cervix and uterine tubes samples were obtained at slaughter. Cornu uteri were washed in phosphate buffer solution (each animal contained at least one embryo). Tissues were fixed in 10% neutral buffered formol, Carnoy solution and Mota's fixative (basic lead acetate) for 48 h and embedded in paraffin. Six-micrometre-thick sections were stained with Congo red (for eosinophil granulocytes) and toluidine blue in 1% aqueous solution at pH 1.0 for 5 min (for MCs). In the uterus, MCs occurred in highest numbers in the myometrium. Higher MC numbers were observed in uterus, uterine and uterine tubes in the preimplantation (experimental) group (cycle synchronised through 7 days intravaginal sponge with 0.3 g P-4) compared with the control group (P < 0.05). Eosinophil granulocyte numbers were significantly higher in the experimental group than in the control group (P < 0.05). These results indicate preimplantation-related changes in numbers of eosinophil granulocytes and MCs in goat reproductive tract organs.
Effect of Spermvital® Technology on Conception Rate in Repeat Breeder Multiparous Dairy Cows: Preliminary Results
(Tubitak Scientific & Technological Research Council Turkey, 2019) Kocyigit, Alper; Uslu, Baris Atalay; Sendag, Sait; Treupel, Elisabeth; Wehrend, Axel
The aim of this study was to investigate the effect of SV technology on conception rates in repeat breeder multiparous dairy cows. Seventy-nine multiparous Holstein cows from a private dairy farm were used in the study. These animals were cows that had failed to conceive from at least 3 regularly spaced services (repeat breeders). Estrus cycles of the cows were synchronized by 2 injections of the PG analogue, administered 11 days apart. GnRH was applied 48 h after the second injection of PG. Twenty-four h after this administration, the animals were randomly divided into 2 groups, control and SV. The animals in the control group (n = 28) were inseminated with standard processed semen, and the cows in the SV group (n = 51) were inseminated with SV (R) technology processed semen. A lower pregnancy rate (35.5%) was determined in the control group than in the SV (47.1%) group. The difference between pregnancy rates in the groups was statistically significant (P < 0.05). We are at too early a stage to say that SV (R) Technology can fully respond to the deficiencies in herd management. This work may also lead to future studies into the use of more animal material.
Effects of Long-Term Release Gnrh Agonist "deslorelin" on Testicular Hsp Expression, Accessory Sex Glands and Testicular Functions in Adult Male Rats
(Elsevier Science inc, 2019) Eski, Funda; Cetin, Nebi; Uslu, Sema; Uslu, Baris Atalay; Sendag, Sait; Yoruk, Mecit; Shakeel, Muhammad
The objective of the present was to determine the effect of long-term release GnRH agonists "deslorelin" on suppression and restoration of testicular and accessory sex glands functions, and expression of HSP in testes of adult male rats. A group of twenty-eight male rats and fifty-six female rats were kept for eleven months. The male rats were subdivided into treatment (n = 18; deslorelin, an analogue of GnRH, 4.7 mg, S.C; six months) and control (n = 10; untreated), and the adult female rats were introduced with either treatment or control male rats at the 2nd, 6th and 11th months post implant insertion. At 6th month of deslorelin implants insertion, six male rats from treatment and five rats from control group were sacrificed. The remaining (twelve treatment and five control) male rats were sacrificed at 11 months. The testicular dimension were measured monthly in both treatment and control rats. The blood samples were collected for testosterone and HSP70 antibody, whereas, the testes and accessory glands were isolated for histological examination at each sacrificial time. The results showed that testicular dimension were significantly lesser in treatment group until 9 months post treatment. HSP70 protein expression was negligible at 6 months in treatment group but its intensity increased in spermatids 11 months of treatment similar to control group. Significantly lower testosterone concentrations with poor semen quality, and smaller litter size were observed in treatment group. The histological picture of accessory sex glands and seminiferous tubules shown a variable integrity in treatment group than control at 6 months implant insertion. In conclusion, the subcutaneous application of 4.7 mg of the GnRH-analogue deslorelin represents a practicable, like in the female rats, method to suppress testicular, accessory sex glands functions, testicular HSP expression and fertility in male rats. Moreover, the suppressive effects of deslorelin, continued until 11th months after removal of the implant. (C) 2019 Elsevier Inc. All rights reserved.
Effects of Melatonin on the Onset of Ovarian Activity in Turkish Van Cats
(Medwell online, 2009) Gulyuz, Fetih; Tasal, Ibrahim; Kaya, Mustafa; Uslu, Baris Atalay
With the present study, we aimed to investigate the effects of melatonin on the initiation of breeding season in the female cats. For this purpose, 12 female cats and 2 tom-cats (male cats) (vasectomized) at age of 2-6 years old were used. The female cats were randomly divided into control (group 1, n = 6) and melatonin-treated groups (group 2, n = 6). Sham (placebo) and melatonin implants (containing 18 mg of melatonin) were placed subcutaneously into the females during the late anoestrous in groups 1 and 2, respectively. Sexual behaviors, vaginal smears and serum progesterone levels for the animals were recorded. The effect of the daylight (varying from 9.5-12.5 h, December through April) on the cyclic activity were also assessed during the study period. The animals were sexually silent during the anoestrus period. Upon the commencement of breeding season, while the female cats in group I showed estrus signs, none but one of the female cats in group 2 possessed the indications of the estrus. Moreover, the findings of cytology were not statistically different in both groups during the study period (p >= 0.05). Although, the progesterone levels in breeding season were higher in group 1 (p <= 0.05) than in un-breeding season, no such difference was present in group 2. The progesterone levels in breeding season were markedly elevated (p <= 0.01) in group I with comparison to group 2. In the latter group, melatonin effectively postponed cyclic activity of the females. The present results suggest that the administration of exogenous melatonin might prolong un-breeding season, presumably via the suppression of hypothalamic centers. Besides, we found that exposure to daylight of 10.5 or more hours per day were also, sufficient for the onset of cyclic activity in the female cats.
Effects of Oestrus Synchronisation Using Melatonin and Norgestomet Implants Followed by Ecg Injection Upon Reproductive Traits of Fat-Tailed Morkaraman Ewes During Suckling, Anoestrus Season
(Elsevier, 2012) Uslu, Baris Atalay; Tasal, Ibrahim; Gulyuz, Fetih; Sendag, Sait; Ucar, Omer; Goericke-Pesch, Sandra; Wehrend, Axel
The aim of this study was to compare the efficacy of different oestrus synchronisation protocols using melatonin (MEL), norgestomet (NOR) and melatonin + norgestomet (MELNOR) treatments (further, all animals from the groups MEL, NOR and MELNOR were supplemented with equine chorionic gonadotropin, eCG) in fat-tailed Morkaraman ewes during the non-breeding season. Forty healthy suckling ewes were assigned, from mid-May onwards, randomly to one of four experimental groups. Ten ewes were assigned to the control group CON (n = 10) and received no treatments. Ten ewes were assigned to the MEL group (n = 10) receiving a subcutaneous melatonin ear implant for 35 days, followed by a 500 IU intramuscular (i.m.) injection of eCG. Ten ewes were assigned to the NOR group (n = 10) and received norgestomet implants placed subcutaneously in the ear for 10 days and followed by a 500 IU i.m. injections of eCG after removal of the implant. The last 10 ewes were assigned to the MELNOR group (n = 10) receiving a subcutaneous melatonin ear implant for 35 days plus a subcutaneous norgestomet ear implant, inserted on the 25th day of the melatonin implant, for 10 days. It was then followed by a 500 IU i.m. injection of eCG after removal of the norgestomet implant and by introduction of rams to the flock concurrently. Ewes exhibiting oestrous signs by 7 days were hand-mated with rams known to be fertile. Blood samples were collected at the time of first implantation (baseline), on day 35 (norgestomet implant removal and eCG injection) and when ewes expressed oestrus in order to determine plasma progesterone (P-4) concentrations. The values of oestrus response, onset of oestrus post-treatment, pregnancy/lambing rates and litter sizes were recorded. The results showed that the oestrus rates were identical for all synchronisation protocols (100%) and the oestrus rates (40 +/- 16.3%) were significantly lower (P < 0.001) in the control group. The onset of oestrus shortened slightly in the hormone-treated groups. The P-4 concentrations on the day of eCG injections (at the end of synchronisation) were significantly higher (P < 0.01) in the hormone-treated groups (varying from 2.45 to 2.91 ng/ml) compared to the control group (0.45 ng/ml). Pregnancy/lambing rates were slightly higher in the synchronised groups (varied from 60 to 80%), compared to the control group. The results of oestrus synchronisation with melatonin and norgestomet ear implants (supplemented with the eCG) showed that: (i) an oestrus onset could be shortened significantly (P < 0.001) with melatonin and/or norgestomet, and (ii) significantly higher (P > 0.01) P-4 concentrations and higher lambing rates could be obtained in all hormone-treated ewes when compared to the un-treated Morkaraman ewes during the suckling (anoestrus) season. (C) 2012 Elsevier B.V. All rights reserved.
Electroejaculation in Impotent Rams and Artificial Insemination in Ewes
(indian veterinary Journal, 2006) Kaya, Mustafa; Gulyuz, Fetih; Uslu, Baris Atalay; Tasal, Ibrahim
This research was carried out to evaluate the efficiency of semen collection by the electroejaculation, method from Kivircik impotent rams and Al using fresh semen in Akkaraman ewes.
Estrus Synchronisation With Prid and Calving Rate in Cows
(indian veterinary Journal, 2006) Kaya, Mustafa; Gulyuz, Fetih; Uslu, Baris Atalay
The objective, of the. study was to determine best time: for Al, the effect of. timing of Al on calving raties-and to improve of pregnancy rates after estrus synchronization with PRID in cow and heifers in natural condition.
Investigation of the Immunocastration Efficacy and Longevity of a Single Dose Gnrh Vaccine in Young Male Rats
(Parlar Scientific Publications (p S P), 2019) Eski, Funda; Mis, Leyla; Tasal, Ibrahim; Uslu, Baris Atalay; Comba, Bahat
Conventionally, immunization protocol require an initial priming immunization followed by an antigen boost for adequate immunity and long-term immunization. In this study we were evaluate the immunocastration efficacy and longevity of immunization with a single-dose GnRH vaccine (Repro-Bloc (TM)) in young male rats. Rats in the vaccine group (VAC, n: 35) were treated with GnRH vaccine as single-dose (200 mu l) and the control group (C, n: 15) were also injected with saline solution. Blood samples from all rats were taken monthly for 6 months and at 12th month. GnRH antibody titers, testosterone and leptin levels were determined by commercial RIA kits. Rats at 6th and 12th months were sacrificed for histologic analysis of testes. GnRH antibody levels were found similar in the VAC and C groups (P>0.05) at -1 week. As compared to the C, GnRH antibody levels (pg/ml) of the VAC group at 1st, 2nd, 3rd and 4th months rose continuously and reached the peak level at 5th month, decreased at 6th month and increased again by the 12th month (P<0.05), whereas in the C group GnRH antibodies weren't different (P>0.05). Compared to the unchanged testosterone levels within the process in C group, serum testosterone levels of the VAC group were lower at 2nd month and higher at 3th month, and thereafter lower (P<0.05). Leptin levels were no difference between groups (P>0.05). There were significant differences in the testicle weights, sperm motility, sperm numbers and abnormal sperm rates between VAC and C groups at 6th and 12th months (P< 0.05). Histological examination showed no significant difference in testes between groups at 6th and 12th months (P>0.05). In conclusion, although the vaccine reduced the germinative and endocrine testicular function from 6th months to 12th months with the single-dose GnRH vaccine, the single-dose vaccination did not provide adequate effect in the genital organs in male rats. Further studies are needed to determine an optimal single-dose, longevity of the vaccine and route of administration for complete suppression on the genital organs in different animal species.
Is There a Relationship Between Serum Minerals (Ca, Mg) and Trace Elements (Cu, Fe, Mn, Zn) at Mating on Pregnancy Rates in Fat-Tailed Morkaraman Sheep
(Agricultural Research Communication Centre, 2017) Uslu, Baris Atalay; Mis, Leyla; Gulyuz, Fetih; Comba, Bahat; Ucar, Omer; Tasal, Ibrahim; Wehrend, Axel
The specific roles of nutrients in reproduction are not well defined in sheep. Hence, the relationships between certain mineral (Ca, Mg) and element (Cu, Fe, Mn, Zn) levels at mating and pregnancy rates was investigated in fat-tailed Morkaraman ewes synchronised with different hormones (melatonin, norgestomet implant and eCG) early in anoestrus season. For this purpose, 40 healthy ewes (aged 2-4 years) allocated in the synchronised (n=30) and no-treated group (n=10) were used during mid-May (suckling period). On the eCG injection day, fertile rams (n=8) were introduced into the groups and, then the oestrus signs were visually monitored twice daily for 7 days. The oestrous ewes were allowed to hand-mate using rotational (four rams daily) mating system. Additionally, jugular blood samples were collected on the days of onset of oestrus (before mating) for determination of serum Ca, Cu, Fe, Mg, Mn and Zn levels. Presence of pregnancies was diagnosed by real-time ultrasonography 35 days post-mating. Totally, 25 ewes became pregnant (21 ewes with stimulated-and 4 with spontaneous oestrus), while 9 ewes (all received stimulation) were not pregnant. In non-pregnant ewes, unlike other minerals and elements, the Cu and Zn values were significantly lower than their reference values (for Cu: 0.87 +/- 10.16 mg/dl vs. 9.20 mg/dl; for Zn: 2.06 +/- 10.30 mu g/dl vs. 3.8 mu g/dl, resp.; P<0.01 in both cases). In pregnant ewes, the corresponding values remained unchanged (P>0.05). Hence, the present findings indicate that the low Cu and Zn levels during the oestrus might adversely affect the subsequent pregnancy rate in suckling ewes.
Protective Effects of Silymarin on Methotrexate-Induced Damages in Rat Testes
(Univ Sao Paulo, Conjunto Quimicas, 2018) Yaman, Turan; Uyar, Ahmet; Kaya, Mehmet Salih; Keles, Omer Faruk; Uslu, Baris Atalay; Yener, Zabit
The present study aimed to investigate the protective effects of silymarin (SMN), an antioxidant, on methotrexate (MTX)-induced damage in rat testes. Thirty-two Wistar albino rats were divided into four groups (n = 8): control, MTX (20 mg/kg, i.p. on days 1 and 5), SMN (200 mg/kg, orally), and MTX + SMN (20 mg/kg, i.p. on days 1 and 5 and SMN 200 mg/kg orally) groups. At the end of the 6-week trial period, histopathological, immunohistochemical, biochemical, and spermatological analyses were performed on testes tissues. Histopathologically, MTX-induced damage, including depletion of germ cell and loos of spermatozoa, was significantly improved with SMN treatment. Immunohistochemically, the immunoreactivity of glutathione peroxidase 1 (GPx1) and manganese superoxide dismutase 2 (SOD2) were detected more intensely in the MTX + SMN group than in the MTX group. Biochemical examinations revealed that SMN supplementation decreased the lipid peroxidation and increased enzymatic antioxidants in the SMN-treated rats. Spermatologically, significant differences were found in the density, motility, dead-to-live sperm ratio, and abnormal sperm rate in the MTX + SMN group compared to the MTX group. In conclusion, SMN seems to have protective effects as an antioxidant against MTX-induced damage in rat testes.
A Retrospective Examination of Reproduction and Survival Rates of Van Cats
(Agricultural Research Communication Centre, 2017) Cak, Bahattin; Uslu, Baris Atalay; Demirel, Ahmet Fatih; Gulyuz, Fetih; Yilmaz, Orhan; Kosal, Volkan
Van cats are animals that often become the centre of attention due to their white hair, colourful eyes, and affectionate attitudes. However, it is nearly impossible to find research studies on the reproductive behaviours of Van cats. We conducted this study with the purpose of revealing the breeding efficiency properties and survival rates of Van cats. The study materials consisted of Van cats in the Van Cat Home of Yuzuncu Yil University's Van Cats Research Center. We determined the breeding efficiency properties using records obtained from 56 female cats over the course of four years. As a result of the study, we determined that the durations of oestrus, pregnancy, and lactation in Van cats were, respectively, 2.41 +/- 0.09, 63.75 +/- 0.27, and 98.45 +/- 1.18 days. We also determined the average number of kittens per delivery and the mating per pregnancy as, respectively, 3.30 +/- 0.19 and 13.71 +/- 0.74. In this study, we recorded the presence of 56 delivering cats, with a total of 185 kittens, 22 of which were dead (11.89%), and an 88.11% survival rate (%). The most common delivery type was twin deliveries (30%), and the highest rate of stillbirths was 4.86% in quadruplet deliveries.
Short-Acting Deslorelin Implant (Ovuplant®) Could Not Sufficiently Induce Fertile Cycles of Coloured Mohair Goats in Suckling (Anoestrous) Season
(Kafkas Univ, veteriner Fakultesi dergisi, 2012) Uslu, Baris Atalay; Sendag, Sait; Tasal, Ibrahim; Gulyuz, Fetih; Ucar, Omer; Wehrend, Axel
It is known that the pituitary FSH and LH stores are dramatically depleted during the anoestrous in seasonal breeders. Hence, the aim of this study was to evaluate the effect of deslorelin implant (a GnRH analogue) to induce oestrus in suckling goats. Suckling goats (n=21) of coloured Mohair breed were randomly assigned into two trial groups: Goats in Group I (n=11, Ovuplant (R)) received subcutaneously the implant (2.1 mg deslorelin), while animals in Group II (n=10, control) received no treatment. Along with the implant administration, serum progesterone (P-4) concentrations were determined (every other day up to 12 d) by RIA method. Within 4 days of implant administration, there were significantly higher (P<0.001) rates (100%) of pro-oestrus signs (attractiveness without mating) in Group I, than those in controls (zero %). The signs sustained for 9 days without oestrus (mating) in the vast majority (10/11) of implant-treated animals. But, an exceptional goat was in receptive oestrus commenced from the third day following the onset of pro-oestrus. Even so, there was no pregnancy post-mating. The P-4 concentrations between the groups remained below 1 ng/ml, regardless of implant treatment. Findings suggest that; i) for the P-4 concentrations, there was no marked difference between implant-treated and control groups, as remaining below 1 ng/ml all, and ii) short-acting deslorelin implant could not induce fertile oestrus cycles in coloured Mohair goats during the suckling (anoestrous) season.