Browsing by Author "Uslu, S."

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Age-Related Changes in the Number of Mast Cells in the Avian Lymphoid Organs
(Wiley-blackwell Publishing, inc, 2006) Karaca, T.; Yoruk, M.; Uslu, S.
The distribution of mast cells (MCs) was studied in the lymphoid organs (thymus, bursa of Fabricius and spleen) of 0-, 7-, 21-, 30- and 120-day-old chickens, using light microscopic histochemical techniques. Tissues samples were obtained under deep anaesthesia from animals in five groups. Tissues were fixed in Mota's fixative (basic lead acetate) for 24 h and embedded in paraffin. Six-micrometre-thick sections were stained with toluidine blue in 0.5% aqueous solution at pH 1.0 for 5 min and Alcian blue/Safranine at pH 1.42 for 30 min. MCs were found in the organs, mostly associated with sinuses and blood vessels. A large increase in MCs was observed in both thymus and spleen of 21-day-old chickens compared with 0-, 7-, 30- and 120-day-old chickens. However, in the bursa of Fabricius, numbers of MCs were significantly higher in the 7-day-old group compared with other age groups. Safranine-positive MCs were not observed in all organs and age groups. These results showed age-related changes in the number of MCs in avian lymphoid tissues.
The Densities of Fiber Follicles in the Karakaş, Norduz, and Zom Sheep and a Comparative Analysis
(Universidad Austral de Chile, 2018) Tuncer, S.S.; Uslu, S.; Taş, A.; Şireli, H.D.
This study aimed to determine the densities of fiber follicles in the skin of the Karakaş, Norduz, and Zom sheep breeds and to carry out a comparative analysis between them. The study included a total of 36 animals aged 1-2 years and 3-4 years. Skin samples were obtained through biopsies taken from the right sides of the animals to determine the densities and ratios of the fleece follicles. Examination of these samples revealed that the primary follicle densities of the three sheep breeds (Karakaş, Norduz and Zom) were 3.11 ± 0.42, 3.14 ± 0.49 and 3.34 ± 0.59, while the secondary follicle densities were 8.42 ± 1.48, 8.51 ± 1.54 and 6.36 ± 1.44. The secondary to primary (S/P) follicle ratios of the sheep were 2.71 ± 0.70, 2.79 ± 0.69 and 1.85 ± 0.36, respectively. The primary follicle densities of the Karakaş, Norduz and Zom sheep were statistically similar for both age groups. The density of secondary follicles and S/P ratio decreased with age in both age groups. The similarity of the S/P ratios to those from other local sheep breeds suggest that Karakaş, Norduz, Zom sheep can be included in the sheep group that has coarse fleece. © Universidad Austral de Chile. All rights reserved.
Distribution and Quantitative Patterns of Mast Cells in Ovary and Uterus of Rat
(Univ Austral Chile, Fac Ciencias veterinarias, 2007) Karaca, T.; Yoeruek, M.; Uslu, S.
The aim of this study was to evaluate the distribution of mast cells in the ovary and uterus of rats during their oestrous cycle. Fourty female Wistar Albino rats, 10-12 weeks old, were used. Ovary and uterus tissues were fixed in Mota's fixative for 48 It and embedded in paraffin. Sections of 6 mu m thickness were cut and stained with toluidine blue (1% aqueous solution) and Alcian blue-safranin (pH: 1.0, buffered by using 0.1 N HCl. In the ovary, the mast cells were mainly present in the tunica albuginea, in the interstitial areas between follicles or corpora lutea and in the vicinity of blood vessels in the medulla. The number of mast cells in the ovarian medulla and cortex and the uterine endometrium and myometrium were highest in the oestrous. metaoestrous, oestrous and metaoestrous phases, respectively. The number of mast cells was higher in the ovarian medulla (7.4 +/- 0.52) and cortex (2.1 +/- 030) during oestrus rather than in other phases of the oestrous cycle (P < 0.05), with the highest number in the endometrium during the oestrous (6.8 +/- 0.45) and metaoestrous (6.2 +/- 0.56) and in the myometrium (7.1 +/- 0.63 for oestrous, 7.5 +/- 0.33 for metaoestrous) of uterus compared to other phases of the cycle (P < 0.05). Safranin-stained mast cells were numerous in all tissues during all stages of the oestrous cycle except the proestrous. Mast cells were safranin positive in the myometrium only during proestrous. It was concluded that some physiological changes could be responsible for the variation in the distribution of mast cells in the ovarian and uterine tissues of the rat during the oestrus cycle.
The Effect of Plantago (Plantago Lanceolata) Added Into Diets at Specific Proportions Upon Distribution of Mast Cells of Digestive System Organs in Male Quails
(Ataturk Universitesi, 2016) Uslu, S.; Temur, C.; Yörük, M.
This research was performed to determine the effect of plantago (Plantago Lanceolate) added 0, 1, 3, 5% into diet groups (n=6 each) on the number of mast cells in some organs of the digestive system in male quails (n=24, in total). In digestive system of quails, the appropriate pieces of tissue samples were taken from the proventriculus, duodenum, jejunum and ileum, and fixed in Mota's basic lead asetat solution for 24 h and after histological tissue processing, embebbed in paraplast. The sections of 6 μ in thickness were stained with toludine blue (0.5%) at pH 0.5. When distributions of mast cells in the lamina propria, submucosa, tunica muscularis plus tunica serosa were examined, it was observed that most of the mast cells were located within the submucosal layer. In the organs examined, the highest number of mast cells were found in the proventriculus. It was determinded that plantago added into diets increased the number of mast cells in male quails.
The Effect of Royal Jelly on Cd3+, Cd5+, Cd45+ T-Cell and Cd68+ Cell Distribution in the Colon of Rats With Acetic Acid-Induced Colitis
(Elsevier Espana Slu, 2012) Karaca, T.; Simsek, N.; Uslu, S.; Kalkan, Y.; Can, I.; Kara, A.; Yoruk, M.
Background: Traditional medicines and health supplements have historically been used to treat many illnesses but most of them have not been evaluated objectively to prove their efficacy. We have been investigating the effects of royal jelly (RJ) supplements on acetic acid-induced colitis on the distribution of CD3(+), CD5(+), CD45(+) T-cell and CD68(+) cells in rats. Methods: The rats were divided into four equal groups: control group, royal jelly-treated (RJ - 150 mg kg(-1) body weight), acetic acid-treated (colitis) and acetic acid-treated (colitis) + royal jelly (CRJ - 150 mg kg(-1) body weight). Colitis was induced by intracolonic instillation of 4% acetic acid; the control group received physiological saline (10 mL kg(-1)). Colon samples were obtained under deep anaesthesia from animals in four groups. Tissues were fixed in 10% formalin neutral buffer solution for 24 h and embedded in paraffin. Results: The proliferative response of CD3(+) and CD45(+) T cells stimulated with colitis was affected by colitis treated with RJ. No differences were found in CD5(+) T cells and CD68(+) macrophages in the colitis treated with RJ. Conclusions: This study has shown that RJ has anti-inflammatory and cell regeneration effect in the colon of rats with acetic acid induced colitis. (c) 2011 SEICAP. Published by Elsevier Espana, S.L. All rights reserved.
Effect of Royal Jelly on Experimental Colitis Induced by Acetic Acid and Alteration of Mast Cell Distribution in the Colon of Rats
(Pagepress Publ, 2010) Karaca, T.; Bayiroglu, F.; Yoruk, M.; Kaya, M. S.; Uslu, S.; Comba, B.; Mis, L.
This study investigated the effects of royal jelly (RI) on acetic acid-induced colitis in rats. Twenty adult female Wistar albino rats were divided into four treatment groups of 5 animals each, including a control group (Group I); Group II was treated orally with RJ (150 mg kg(-1) body weight); Group III had acetic acid-induced colitis; and Group IV had acetic acid-induced colitis treated orally with RJ (150 mg kg(-1) body weight) for 4 weeks. Colitis was induced by intracolonic instillation of 4% acetic acid; the control group received physiological saline (10 mL kg(-1)). Colon samples were obtained under deep anaesthesia from animals in all groups. Tissues were fixed in 10% formalin neutral buffer solution for 24 h and embedded in paraffin. Six-micrometre-thick sections were stained with Mallory's triple stain and toluidine blue in 1% aqueous solution at pH 1.0 for 5 min (for Mast Cells). RI was shown to protect the colonic mucosa against the injurious effect of acetic acid. Colitis (colonic damage) was confirmed histomorphometrically as significant increases in the number of mast cells (MC) and colonic erosions in rats with acetic acid-induced colitis. The RI treatment significantly decreased the number of MC and reduced the area of colonic erosion in the colon of RJ-treated rats compared with rats with untreated colitis. The results suggest that oral treatment with RI could be used to treat colitis.
Effects of Plantain (Plantago Lanceolata) Containing Diets of Quails on Growth Performance, Carcass Characteristic, Some Blood Parameters and Mast Cell Numbers
(Centenary University, 2019) Temür, C.; Uslu, S.
Plantain (Plantago lanceolata) is used for medicinal purposes as a preventive, therapeutic and metabolic regulator due to its bioactive compounds. With the purpose of determine the effect of this plant on quail, 240 of these 1 day old quail chicks were divided into 4 groups, control, added plantain into rations as 1%, 3% and 5%. Body weight gain, feed intake carcass characteristics, internal organ measurements, blood parameters and mast cells were determined. At the end of the study, the hot carcass yield, cold carcass weight and yield were high in the 1%, gizzard weight in 5% plantain added group (p<0.05). The total amount of blood protein was the highest in 3% added group (p<0.05). Blood glucose levels were high in all treatment groups compared to the control group (p<0.05). The amount of T-testosterone was higher than the others in the control and 3% plantain groups (p<0.05). The total number of mast cells increased in the 5% plantain group (p<0.05). it was understood that the addition of plantain to quail rations could have a positive effect, but bioactive components and secondary metabolites of plantain and its extracts need identification and isolation by in vitro and in vivo studies for determining effects on metabolism. © 2019, Centenary University. All rights reserved.
Endocrine and Cytological Responses To Oral Administration of Altrenogest at the Onset of the Breeding Season in Coloured Mohair Goats
(Wiley-blackwell, 2014) Uslu, S.; Belhan, S.; Uslu, B. A.; Cetin, N.; Yoruk, M.; Gulyuz, F.; Wehrend, A.
Investigation of Desensitization Effect of Deslorelin Implant (4.7 Mg) in Young Male Rats
(Wiley-blackwell, 2014) Eski, F.; Uslu, S.; Cetin, N.; Sendag, S.; Uslu, B. A.; Yoruk, M.; Wehrend, A.
Investigation of the Effect of Gnrh (Deslorelin) on the Histochemical Structure of Salivary Glands in Rats
(Universidad de la Frontera, 2019) Uslu, S.; Çakmak, G.; Yörük, M.
This study was planned to determine the histochemical alterations of the submandibular gland by implantation of long-term GnRH (deslorelin 4.7 mg). Eighteen Wistar albino rats were used in the study. Alcian blue (AB; pH: 2.5), periodic acid–Schiff (PAS) staining was performed to determine the microscopic structure and histochemical structure of the GI submandibular gland. The Avidin–Biotin Complex (ABC) method was used to determine the immunohistochemical reactivity of lectin. After GnRH implantation, the organs were examined and atrophies were observed in organs. In the group in which the implants were removed, it was determined that there was no atrophy; organ structures and microscopic examination were similar to the control group. At the end of the study, submandibular gland was fixed in 10 % buffered formaldehyde. In three groups, PAS and AB histochemical staining revealed similar reactions. Immunohistochemically, lectin activity was found to react positively. © 2019, Universidad de la Frontera. All rights reserved.
Investigation of the Localisation of Estrogen Receptor Alpha and Progesterone Receptor B in the Ovaries of Goats During and Out of the Breeding Season
(Wiley-blackwell, 2015) Uslu, S.; Yoeruek, M.; Mis, L.; Comba, B.; Sendag, S.; Wehrend, A.