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Browsing by Author "Uslu, Ugur"

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    First Report of Zoonotic Cryptosporidium Parvum Subtype Iiaa15g2r1 in Dogs in Türkiye
    (Univ Agriculture, Fac veterinary Science, 2024) Ayan, Adnan; Celik, Burcak Aslan; Celik, Ozgur Yasar; Akyildiz, Gurkan; Kilinc, Ozlem Orunc; Ayan, Ozge Oktay; Uslu, Ugur
    Cryptosporidium (C.) is an opportunistic protozoan causing gastrointestinal illness in both humans and animals, leading to acute or chronic diarrhea and even death. The study aimed to investigate the prevalence and subtyping of Cryptosporidium spp. in shelter dogs in Van province, T & uuml;rkiye. For microscopic identification of this parasite, a total of 300 fecal samples were collected and stained with Kinyoun's acid-fast method. For molecular analysis, the positive samples were subjected to DNA extraction and SSU rRNA gene of Cryptosporidium spp. was amplified using nested PCR. The microscopic examination revealed a 4.67% prevalence of Cryptosporidium spp. Sequence analysis indicated all samples were positive to C. parvum. In addition, GP60 gene was also amplified and C. parvum subtypes IIaA15G2R1 was confirmed by analyzing the obtained sequences. All the sequences of SSU rRNA and GP60 were deposited in GenBank. To our knowledge, Cryptosporidium parvum subtypes IIaA15G2R1 have been reported first time in dogs in T & uuml;rkiye. It is recommended to implement control strategies by awareness campaign, preventing stray dogs from freely entering public areas, and proper disposal of dog feces.
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    Prevalence and Molecular Characterization of Moniezia Species in Ruminants Based on Its1-5.8s Rrna From Van Province, Turkey
    (Univ Agriculture, Fac veterinary Science, 2025) Yilmaz, Ali Bilgin; Azizoglu, Erkan; Adizel, Ozdemir; Goz, Yasar; Celik, Burcak Aslan; Celik, Ozgur Yasar; Uslu, Ugur
    This study aimed to calculate the occurence of Moniezia species in cattle, sheep, and goats in Van province and to identify these species using morphological and molecular methods (ITS1-5.8S rRNA gene region analysis). Additionally, the study aimed to identify the genetic differences between Moniezia expansa and Moniezia benedeni. During the summer of 2022, intestinal contents were collected from 150 ruminants (50 cattle, 50 sheep, and 50 goats) slaughtered in slaughterhouses in Van province. The parasites were examined using Aceto-Carmine staining, and species identification was based on interproglottidal glands. Examination of the intestinal contents revealed that 2 out of 50 cattle (4%), 14 out of 50 sheep (28%), and 9 out of 50 goats (18%) were infected with Moniezia. Morphological and molecular analyses showed that the cattle samples were identified as Moniezia benedeni, goats samples as Moniezia expansa and those from sheep as 11 Moniezia expansa and 3 Moniezia benedeni. Following DNA extraction, the ITS1-5.8S rRNA gene region was amplified using PCR and subjected to sequence analysis. The relationship between species was examined by phylogenetic tree. This study confirms the prevalence of Moniezia spp. in Van/T & uuml;rkiye by using the ITS1-5.8S rRNA gene.