Browsing by Author "Uyar, Ahmet"

Now showing 1 - 20 of 34

Biomedical Effects of Laurus Nobilis L. Leaf Extract on Vital Organs in Streptozotocin-Induced Diabetic Rats: Experimental Research
(Elsevier Sci Ltd, 2021) Mohammed, Rebin Rafaat; Omer, Abdullah Khalid; Yener, Zabit; Uyar, Ahmet; Ahmed, Avin Kawa
Diabetes mellitus (DM) has been treated with herbs for centuries and many herbs reported to exert antidiabetic activity. Laurus nobilis is an aromatic herb belonging to the Lauraceae family, commonly known as bay. This study aimed to investigate the activity of Laurus nobilis leave extracts on histopathological and biochemical changes in beta-cells of streptozotocin (STZ)-induced diabetic rats. Thirty healthy adult male albino rats were included in the study and divided equally into 5 groups for 4 weeks as follow; control group (C), diabetic group (D), diabetic Laurus nobilis extract group (DLN), Laurus nobilis extract group (LN) and diabetic acarbose (DA) group. Histopathologically, D group rats exhibited various degenerative and necrotic changes in their liver, pancreas and kidney, whereas the DLN rats had nearly normal histology. Insulin immunostaining in the pancreatic beta cells was decreased in the D group compared to the C group, whereas the DLN group was similar to the C group. The glucose concentration decreased significantly in both diabetic rats treated with L. nobilis and acarbose (p < 0.05). Additionally, the levels of aspartate aminotransferase (AST), gamma-glutamyltransferase (GGT) and alanine aminotransferase (ALT) enzyme were significantly decreased in both diabetic rats treated with L. nobilis and acarbose, compared to the D group (p (>) 0.05). Outcomes of this study said that leave extracts of L. nobilis has valuable effect on blood glucose level and ameliorative effect on regeneration of pancreatic islets, it also restored the altered liver enzymes, urea, creatine kinase, total protein levels, calcium and ferritin to near normal.
Chemopreventive Efficacy of Juniper Berry Oil (Juniperus Communis L.) on Azoxymethane-Induced Colon Carcinogenesis in Rat
(Routledge Journals, Taylor & Francis Ltd, 2021) Yaman, Turan; Uyar, Ahmet; Komuroglu, Ahmet Ufuk; Keles, Omer Faruk; Yener, Zabit
The aim of this study was to investigate the chemopreventive effects of juniper berry (JB) oil on azoxymethane (AOM)-induced colon cancer in rats. Thirty-two male Wistar albino rats were allocated into four groups: Control, AOM, AOM?+?JB, and JB groups. Whereas the control group was fed with standard pellet feed, the AOM and AOM?+?JB groups were administered of AOM (15?mg/kg body weight) subcutaneously once every 2 weeks for 10?weeks. AOM?+?JB and JB groups additionally received JB oil (100??l/kg) orally. At the end of the 16-week experimental period, blood and tissue samples were obtained from the rats following necropsy. The macroscopic findings showed that the application of JB oil significantly decreased adenoma and adenocarcinoma formation both numerically and dimensionally. Immunohistochemically, CEA, COX-2, and Ki-67 immune-expressions decreased, and the immune-expression of caspase-3 increased in AOM?+?JB treated rats. Additionally, JB oil supplementation ameliorated antioxidant defense systems and lipid peroxidation within the colon tissue of AOM?+?JB treated rats. These results reveal that the JB oil acted as a chemopreventive dietary agent, inhibiting cell proliferation and COX-2 expression and inducing apoptosis, resulting in a significant reduction in colon tumor formation.
Congenital Extraneural Hemangioblastoma in a Lamb
(Sage Publications inc, 2019) Yaman, Turan; Karasu, Abdullah; Uyar, Ahmet; Kuscu, Yagmur; Keles, Omer Faruk
A 1-mo-old Ivesi male lamb was presented with 2 large red masses on the skin of the left ear. The tumors were removed using gentle dissection and submitted for histologic evaluation. The tumors consisted of numerous thin-walled capillaries lined by endothelial cells and nests of stromal cells. Immunohistochemically, the endothelial cells were positive for CD45, and the stromal cells were positive for neuron-specific enolase. GFAP-positive cells were occasionally present within the tumor. Endothelial and stromal cells were negative for S100, CD34, CD31, and factor VIII-related antigen. The tumor had strong gross, microscopic, and immunohistochemical similarities with human extraneural hemangioblastoma.
Deneysel Ülseratif Kolit Üzerine Yüksek Karbonhidratlı, Yüksek Yağlı ve Aralıklı Beslemenin Etkisi
(2018) Yaman, Turan; Emlik, Hüseyin; Uyar, Ahmet
Bu çalışmada, dünyada görülme sıklığı artan inflamatuvar bağırsak hastalıklarının (İBH) bir tanesi olan Ülseratif kolit (ÜK)hastalığının Wistar albino türü ratlarda deneysel oluşumu üzerine beslenme şekli ve sıklığının karşılaştırmalı olarak araştırılmasıamaçlanmıştır. Bu amaçla; Kontrol (n=7), Kolit (n=7), Kolit-Yüksek Karbonhidratlı Diyet (K-YKD) (n=7), Kolit–Yüksek KarbonhidratlıAralıklı Diyet (K-YKAD) (n=7), Kolit–Yüksek Yağlı Diyet (K-YYD) (n=7), Kolit-Yüksek Yağlı Aralıklı Diyet (K-YYAD) (n=7) olacak şekildeçalışma grupları oluşturuldu. Gruplardan aralıklı besleme gruplarına haftada sadece 2 gün (ardarda olmayan) diyet verilmesine 24 saatara verildi. 7 haftalık beslemeden sonra deneysel kolit modeli; kolit ve tedavi gruplarındaki (Kolit, K-YKD, K-YKAD, K-YYD, K-YYAD)ratlara anestezi altında 30o trendelenburg pozisyonunda 8 mm’lik kateterin rektal yoldan 8 cm ileriye asetik asidin (pH 2.4, % 4)intrarektal (i.r.) olarak uygulanması ile oluşturuldu. 72 saat sonra ratlar sakrifiye edilerek histopatolojik örnekler %10 tamponluformaldehite alındı. Histopatolojik incelemelere göre asetik asitin ciddi kolit hasarlarına neden olduğu buna karşın aralıklı olarak verilendiyet gruplarında bu hasarların oldukça minimal düzeyde kaldığı görülmüştür. Sonuç olarak kolit modelinde aralıklı diyet uygulamasınınolumlu etkileri olduğu kanısına varıldı.
Detection of Bovine Respiratory Syncytial Virus, Pasteurella Multocida, and Mannheimia Haemolytica by Immunohistochemical Method in Naturally-Infected Cattle
(Walter de Gruyter Gmbh, 2018) Yaman, Turan; Buyubayram, Huseyin; Ozyildiz, Zafer; Terzi, Funda; Uyar, Ahmet; Keles, Omer Faruk; Yener, Zabit
Introduction: The aim of this study was to determine the predisposing effect of bovine respiratory syncytial virus (BRSV) on Pasteurella spp. infection in naturally-induced pneumonia in cattle by immunohistochemical labelling. Material and Methods: Lungs of cattle slaughtered in the slaughterhouse were examined macroscopically, and 100 pneumonic samples were taken. The samples were fixed in 10% neutral formalin and embedded in paraffin by routine methods. Sections 5 mu m in thickness were cut. The streptavidin-peroxidase method (ABC) was used to stain the sections for immuno-histochemical examination. Results: BRSV antigens were found in the cytoplasm of epithelial cells of bronchi, bronchioles, and alveoles and within inflammatory cell debris and inflammatory exudate in bronchial lumens. Pasteurella spp. antigens were detected in the cytoplasm of the epithelial cells of bronchi and bronchioles, and in cells in the lumens of bronchi and bronchioles. Eleven cases were positive for only one pathogen (six for BRSV and five for Pasteurella spp.), while 35 cases were positive for 2 pathogens: BRSV plus P. multocida (n = 21) or M. haemolytica (n = 14). Conclusion: The presence of high levels of BRSV in dual infections indicates that BSRV may be the main pneumonia-inducing agent and an important predisposing factor for the formation of Pasteurella spp. infections in cattle naturally afflicted with pneumonia.
Effects of Licorice Root (Glycyrrhiza Glabra) Extract on the Livers of Obese Rats
(Taylor & Francis Ltd, 2024) Uyar, Ahmet; Ozdere, Baris; Yaman, Turan; Komuroglu, Ahmet Ufuk
We have investigated anti-obesity effects of the extract of licorice (Glycyrrhiza glabra) root in rats with diet-induced obesity and hyperlipidemia by using histopathological and biochemical methods. Thirty-two Wistar albino rats were divided to four groups of eight: normal control (C), high fat diet (HFD), high fat Diet + Glycyrrhiza glabra (HFD+M), and normal diet with Glycyrrhiza glabra (M). The high fat diet contained 300 g/kg fat (4000 kcal/kg); the daily dosage of Glycyrrhiza glabra extract was 1g/kg body weight by orogastric gavage. Supplementation of Glycyrrhiza glabra extract dramatically reduced increases in body weight caused by the induction of obesity. A hepatoprotective effect of Glycyrrhiza glabra extract was supported by the almost normal histology in the livers of the HFD+M rats, in contrast to the degenerative changes in the HFD rats, which included macrovesicular and microvesicular fat deposits, hydropic degeneration, dilatation of sinusoids and coagulation necrosis of some hepatocytes. Serum levels of alanine transaminase (ALT), aspartic transaminase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), cholesterol (HDL and LDL) and triglycerides, were ameliorated by Glycyrrhiza glabra extract treatment. We conclude that Glycyrrhiza glabra extract given together with HFD could prevent obesity and reduce liver damage in rats.
Evaluation of the in Vitro and in Vivo Effects of the Ethanolic Lyophilized Extract Obtained From Pholiota Aurivella Mushroom on Cci4-Induced Toxicity in Rats
(Parlar Scientific Publications (p S P), 2019) Dogan, Abdulahad; Dalar, Abdullah; Battal, Abdulhamit; Uyar, Ahmet; Sadullahoglu, Cemil; Celik, Ismail; Uzun, Yusuf
The aim of this study was to evaluate Pholiota aurivella extract's chemical and mineral composition, total antioxidant capacity, histopathological changes in liver and kidney, as well as its possible protective/harmful role against carbon tetrachloride (CCl4)-induced oxidative stress in rats. After the toxicity test, twenty four rats were divided into four experimental groups: Control, CCl4, CCl4+P. aurivella (100 mg/kg, extract) and CCl4+P.aurivella (500 mg/kg, extract) groups. Some biochemical parameters such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) in serum, malondialdehyde (MDA) and antioxidant defense system (ADS) constituents such as reduced glutathione (GSH) levels, glutathione reductase (GR), glutathione S-transferase (GST), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities in the liver, brain and kidney tissues, histopathological changes in liver and kidney of rats were observed. P.aurivella extract was rich in phenolic (p-coumaric and protocatechuic acids) and fatty acid (linoleic, oleic and palmitic acids) compounds and high level of Arsenic. In vivo studies revealed that P.aurivella extract suppressed the levels of ADS enzymes and increased the MDA content and AST, ALT and LDH in treated groups. According to histopathological investigation, liver and kidney revealed severe histopathological changes both in CCl4 group and P.aurivella- treated groups. These results have shown that oral administration of P. aurivella extract caused a significant toxic effects in rats. Therefore, in the context of the data obtained from this study the P. aurivella could not be regarded as a safe food source.
Evaluation With Endothelial Nitric Oxide Synthase (Enos) Immunoreactivity of the Protective Role of Astaxanthin on Hepatorenal Injury of Remote Organs Caused by Ischaemia Reperfusion of the Lower Extremities
(Termedia Publishing House Ltd, 2020) Uyar, Ahmet; Yaman, Turan
Introduction: Ischemia and following reperfusion triggers local and systemic damage with the involvement of free oxygen radicals and inflammatory mediators. Although blood flow saves extremity from necrosis,multi organ dysfunction may progress and cause death of the patient. Aim: The study aims to examine the effect of astaxanthin (AST) on the prevention of remote tissue injury resulting from lower extremity ischaemia-reperfusion (I/R). To elucidate the potential hepatoprotective and renoprotective effects of AST, in addition to histopathological findings, the intrahepatic and intrarenal kinetics of endothelial nitric oxide synthase (eNOS) during I/R were determined by using the immunohistochemical method. Material and methods: Twenty-eight male Wistar albino rats were divided into four groups. For the control group, only the anaesthesia procedure (2 h) was conducted without I/R. In the I/R group, 2 h of reperfusion was conducted following ischaemia under anaesthesia. For the I/R group + AST, 7 days prior to ischaemia, 125 mg/kg AST was given with gavage, and 2 h of ischaemia and 2 h of reperfusion were conducted under anaesthesia. Following necropsy, liver and kidney tissue samples were fixed in 10% buffered formalin for 48 h for histopathological and immunohistochemical investigation. Results: The histological analysis revealed that severe I/R hepatorenal injury such as inflammatory cell infiltration, dilatation in sinusoids and lumen of tubuli, congestion in glomerular capillaries, degeneration in hepatocyte and epithelial cells of tubuli, and necrosis was ameliorated by AST. Immunohistochemical studies showed that the I/R-induced elevation in eNOS expression was reduced by AST treatment. Conclusions: In the case of acute lower extremity I/R, AST decreased the ischaemic injury in liver and renal tissues by protecting the microcirculation and providing a cytoprotective effect with vasodilatation.
Examination of Sheep in Which Injection of Yeldif® Induced Sudden Death
(Polish Soc veterinary Sciences Editorial office, 2017) Uyar, Ahmet; Yener, Zabit; Basbugan, Yildiray; Keles, Omer Faruk; Yaman, Turan
Two breeders from the Baskale district of the Van province and the Hizan district of the Bitlis province reported deaths of their lambs one day after injection of a commercial preparation containing a combination of Se and Vit E (Yeldif (R)). Purulent-hemorrhagic-necrotic myositis was detected at the injection site during necropsy of the lambs (2 animals) brought to the Pathology Department of our Faculty by these breeders. This study was conducted to determine whether these post-injection deaths occurred as a result of the drug used or due to a faulty application of the medicine by the breeders. The trial period for this study was 1 week. Eighteen 2-month-old lambs were used in this study. The lambs were divided into three groups (control and 2 study groups) of 6 lambs each. An injection of 1 ml (recommended dose) of the product purchased in the market from the same batch as the one that had caused the deaths was administered I.M. to lambs in the control group. The medicine reported to have caused the deaths by the breeders was administered to the first study group (A) at 1 ml I.M. and to the second study group (B) at 3 ml I.M. (three times the recommended dose). The lambs were bled for biochemical analysis before the administration, on the first day after the administration, and at the end of the trial. Necropsies were performed on dead animals during the trial and on all animals at the end of the trial. At the end of the trial, no clinical-pathological findings were noted in lambs of the control group and group A. However, in all lambs of group B, emphysematous gangrene was detected at the injection site. Cl. chauvoei was isolated and identified in bacteriological and cultural examinations. It was concluded that bacterial contamination occurred as a result of incorrect drug administration by the breeders and injection of a high dose of the contaminated drug.
Exploring of the Ameliorative Effects of Nerium (Nerium Oleander L.) Ethanolic Flower Extract in Streptozotocin Induced Diabetic Rats Via Biochemical, Histological and Molecular Aspects
(Springer, 2023) Battal, Abdulhamit; Dogan, Abdulahad; Uyar, Ahmet; Demir, Abdulbaki; Keles, oemer Faruk; Celik, Ismail; Aslan, Ali
Background Nerium oleander L. is ethnopharmacologically used for diabetes. Our aim was to investigate the ameliorative effects of ethanolic Nerium flower extract (NFE) in STZ-induced diabetic rats.Methods Seven random groups including control group, NFE group (50 mg/kg), diabetic group, glibenclamide group and NFE treated groups (25 mg/kg, 75 mg/kg, and 225 mg/kg) were composed of forty-nine rats. Blood glucose level, glycated hemoglobin (HbA1c), insulin level, liver damage parameters and lipid profile parameters were investigated. Antioxidant defense system enzyme activities and reduced glutathione (GSH) and malondialdehyde (MDA) contents and immunotoxic and neurotoxic parameters were determined in liver tissue. Additionally, the ameliorative effects of NFE were histopathologically examined in liver. mRNA levels of SLC2A2 gene encoding glucose transporter 2 protein were measured by quantitative real time PCR.Results NFE caused decrease in glucose level and HbA1c and increase in insulin and C-peptide levels. Additionally, NFE improved liver damage biomarkers and lipid profile parameters in serum. Moreover, lipid peroxidation was prevented and antioxidant enzyme activities in liver were regulated by NFE treatment. Furthermore, anti-immunotoxic and anti-neurotoxic effects of NFE were determined in liver tissue of diabetic rats. Histopathogically, significant liver damages were observed in the diabetic rats. Histopathological changes were decreased partially in the 225 mg/kg NFE treated group. SLC2A2 gene expression in liver of diabetic rats significantly reduced compared to healthy rats and NFE treatment (25 mg/kg) caused increase in gene expression.Conclusion Flower extract of Nerium plant may have an antidiabetic potential due to its high phytochemical content. [Graphics] .
Histopathological and Biochemical Investigation of the Preventive Effects of Broiler Feeds Including Urtica Dioica Seed Extract Against Aflatoxicosis
(2014) Uyar, Ahmet; Yener, Zabit
ÖZET Uyar A, Broyler Yemlerine Katılan Isırgan Otu Tohumu (Urtica Dioica Seed) Ekstraktının Aflatoksikozise Karşı Koruyucu Etkinliğinin Histopatolojik ve Biyokimyasal Olarak Araştırılması, Y.Y.Ü. Sağlık Bilimleri Enstitüsü, Patoloji Anabilim Dalı Doktora Tezi, Van, 2014. Aflatoksikozis, insan ve hayvanlarda aflatoksinler tarafından oluşturulan bir mikotoksikozisdir. Hayvanlarda önemli ekonomik kayıplara neden olan ve insan sağlığını tehdit eden toksikasyon özellikle geri kalmış ve gelişmekte olan ülkelerde yaygın olarak görülmektedir. Yapılan son çalışmalardan ısırgan otunun immunomodülatör, antioksidan ve antikanserojenik gibi birçok farmakolojik etkilerinin olduğu bilinmektedir. Ancak ısırgan otu tohumu eksraktının aflatoksikozise karşı koruyucu etkileri konusunda broyler yetiştiriciğinde çalışma bulunmamaktadır. Gerçekleştirilen bu çalışmada, broylerlerde deneysel olarak oluşturulan aflatoksikozis'de karaciğer ve böbreklerde meydana gelen lezyonların oluşumunda ısırgan otu tohumu ektraktının engelleyici etkisi; histopatolojik ve biyokimyasal olarak araştırıldı. Bu amaçla, toplam 32 broyler civciv, herbiri 8'erli 4 gruba ayrıldı. Deneme grupları; kontrol grubu (A); normal broyler yemi, IT grubu (B); 30 ml/kg yeme ısırgan otu tohumu ekstresi, AF grubu (C); 1mg/kg AF, AF+IT grubu (D); 1mg/kg AF + 30 ml/kg dozda ısırgan otu tohumu ekstresi ilaveli yemler ile beslendi. Yirmibir günlük deneme sonunda nekropsileri yapılan broylerlerden biyokimyasal ve histopatolojik analizler için kan ve doku örnekleri alındı. Biyokimyasal bulgulara göre sadece IT ekstraksiyonu verilen deneme grubundaki değerlerin kontrol grubuyla benzer olduğu, AF grubunda lipid pereksidasyonun ve karaciğer enzimlerinin önemli ölçüde yükseldiği ve antioksidan savunma sistemlerinin de azaldığı, AF+IT grubunda ise lipid pereksidasyonun ve karaciğer enzimlerinin azaldığı ve kontrol grubuna yaklaştığı, antioksidan sistem aktivitesinin de arttığı gözlendi. Patolojik olarak da C grubunda (AF) saptanan karaciğer ve böbreklerdeki büyüme-solgunluk gibi makroskobik değişiklikler ile hidropik dejenerasyon, safra kanalı proliferasyonu, periportal fibrozis ve perisinüzoidal hücrelerde aktivasyon gibi histolojik bozuklukların IT ekstraktı ilave edilen D grubunda (AF+IT) önemli ölçüde azaldığı ya da oluşmadığı gözlendi. Bu bulgular doğrultusunda broyler aflatoksikoziste, IT ekstraktının lipid peroksidasyonu ve doku hasarını önemli ölçüde engellediği, dolaysıyla koruyucu etkiye sahip olduğu kanısına varıldı. Anahtar sözcükler: Aflatoksikozis, Isırgan otu tohumu ekstresi, Broyler
Histopathological and Immunohistochemical Study of Antidiabetic Effects of Heracleum Persicum Extract in Experimentally Diabetic Rats
(Assoc Pharmaceutical Teachers india, 2017) Yaman, Turan; Uyar, Ahmet; Celik, Ismail; Alkan, Elif Ebru; Keles, Omer Faruk; Yener, Zabit
Objective: This study aims to investigate the antioxidant properties and protective effects of Heracleum persicum (HP) extract in streptozotocin (STZ)-induced diabetic rats. Material and Methods: Forty-two Wistar albino male rats were divided into six groups including Control (C); Diabetes mellitus (DM); DM+Akarboz 20 mg/kg; DM+100 mg/kg HP extract (HP1); DM+200 mg/kg HP extract (HP2) and DM+400 mg/kg HP extract (HP3). Experimental diabetes was established by a single-dose [45 mg/kg, intra-peritoneal (i.p)] STZ injection. Essential dosages of HP extracts and Akarboz were applied with gastric gavage for 21 day. Results: In histopathological evaluation of the stained liver and kidney sections of diabetic rats showed degeneration and necrosis of hepatocytes, inflammatory cell infiltration and hydropic degeneration and necrosis in tubulus epithelial cells, disorder of glomerular structure and lymphocyte infiltration. These histopathological changes were ameliorated in the HP-treated rats depending on the dose level. Glutathione peroxidase 1 (GPx-1) immunoreactivity was detected in hepatocytes of liver and tubule epithelial cells of kidney. We have shown that treatment with extracts of HP modulates GPx-1 expression in HP-treated rats. STZ-induced degenerative changes in beta-cells caused decreases in the number of functioning beta-cells and insulin immunoreactivity in the pancreas of the diabetic rats. The pancreas of HP-treated rats were improved and the number of immunoreactive beta cells were significantly increased. Conclusion: Our data suggests that the STZ-induced immunohistochemical and histopathological alterations could be prevented by HP extract probably due to possess the ability to regenerate beta-cells.
An Immunohistochemical Study on the Presence of Nitric Oxide Synthase Isoforms (Nnos, Inos, Enos) in the Spinal Cord and Nodose Ganglion of Rats Receiving Ionising Gamma Radiation To Their Liver
(Walter de Gruyter Gmbh, 2020) Yilmaz, Osman; Soyguder, Zafer; Keles, Omer Faruk; Yaman, Turan; Yener, Zabit; Uyar, Ahmet; Cakir, Tahir
Introduction: This study determined the presence of nitric oxide synthesis isoforms (nNOS, iNOS, and eNOS) in thoracic spinal cord segments and nodose ganglia of rats with gamma-irradiated livers. Material and Methods: Male rats (n = 32) were divided into equal groups A, B, C, and D. In group A, the controls, no radiation was applied, while groups B, C, and D received 10 Gy of ionising gamma radiation. The rats of group B were euthanized at the end of the first day (d1), those of group C on the second day (d2), and those of group D on the third day (d3). The liver, spinal cord segments, and nodose ganglion tissues were dissected and fixed, and the liver sections were examined histopathologically. The other tissues were observed through a light microscope. Results: Regeneration occurred at the end of d3 in hepatocytes which were radiation-damaged at the end of d1 and d2. On d1, some nNOS-positive staining was found in the neuronal cells of laminae I-III of the spinal cord and in neurons of the nodose ganglion, and on d3, some staining was observed in lamina X of the spinal cord, while none of note was in the nodose ganglion. Dense iNOS-positive staining was seen on d1 in the ependymal cells of the spinal cord and in the glial cells of the nodose ganglion, and on d3, there was still considerable iNOS staining in both tissues. There was clear eNOS-positive staining in the capillary endothelial cells of the spinal cord and light diffuse cytoplasmic staining in the neurons of the nodose ganglion on d1, and on d3, intense eNOS-positive staining was visible in several endothelial cells of the spinal cord, while light nuclear staining was recognised in the neurons of the nodose ganglion. Conclusion: The nNOS, iNOS, and eNOS isoforms are activated in the spinal cord and nodose ganglion of rats after ionising radiation insult to the liver.
Inhibitory Effect of Stinging Nettle (Urtica Dioica L.) Extract on Body Weight Gain in Rats on a High-Fat Diet
(Termedia Publishing House Ltd, 2024) Kacak, Kenan; Yaman, Turan; Uyar, Ahmet; Komuroglu, Ahmet Ufuk
Introduction: The leaves and seeds of Urtica dioica (UD) are used in folk treatments for many diseases. Anticarcinogenic, anti-inflammatory, antioxidant, and antiallergenic properties of UD have been reported. Aim: To uncover the effects of nettle seed (Urtica dioica; UD) extract on body weight gain in rats on a high -fat diet (HFD). Material and methods: Male Wistar albino rats (n = 32) were divided into 4 groups, comprising a control group, a group that received a HFD (HFD group), a group that received UD extracts (UD group), and a group that received a HFD as well as UD extracts (HFD + UD group). UD extracts were given a daily dose of 300 mg/kg of body weight orally for 75 days. Results: The HFD led to weight gain that was partially moderated by the UD extract. Histopathological findings in the HFD + UD group were uniformly significantly lower than those in the HFD group. Serum alanine transaminase, alanine aminotransferase, triglyceride, and low -density lipoprotein levels were significantly higher in the HFD group than in the HFD + UD group, and the HDL levels were lower in the HFD group than in the control group and the HFD + UD group. Conclusions: The cholesterol levels were discovered to be highest in the HFD + UD group. Therefore, it was concluded that the UD extract did not completely protect the rats against body weight gain.
Is Methotrexate-Induced Testicular Damage Preventable Using Nettle Seed Extract: a Histopathological, Immunohistochemical, Biochemical and Spermatological Examination
(Parlar Scientific Publications (p S P), 2018) Uyar, Ahmet; Yaman, Turan; Dogan, Abdulahad; Uslu, Sema; Keles, Omer Faruk; Yener, Zabit; Celik, Ismail
In the study 32 Wistar albino rats were divided into four group as control (Group Control, n=8), methotrexate (Group MTX, n=8), MTX + UDS (Group MTX+UDS, n=8) and Urtica dioica seed extract (UDS) (Group UDS, n=8). After the trial, blood and post-necropsy testicular tissue samples were taken. Histopathological examinations showed that methotrexate had an adverse impact on spermatogenesis by damaging testicles; however, such damages were substantially decreased in the Group MTX+UDS. In the immunohistochemical examinations glutathione peroxidase 1 (GPx1) immunoreactive areas was higher in the Group MTX + UDS compared to the Group MTX. Biochemical examinations revealed that the level of malondialdehyde (MDA), glutathione (GSH), glutathione S-transferase (GST) and catalase (CAT) enzymes levels statistically significantly differenced (p<0.001) in the Group MTX compared to the control, UDS and MTX+UDS groups. There were significant (p<0.05) differences the Group MTX from Group MTX+UDS. such as density, motility, dead-live sperm rate and abnormal sperm rate. Our study results showed that UDS prevented the damage occurred in the testicles according to histopathological, immunohistochemical, biochemical and spermatological findings.
Koyunlarda Erken Anöstrüs Döneminde Malatonin Uygulamalarının Ovulasyon ve Gebelik Üzerine Etkisi
(2008) Alan, Muhammet; Uyar, Ahmet
Bu çalışmada koyunlarda erken anöstrüs döneminde melatonin uygulamalarının ovulasyon ve gebelik üzerine etkisinin araştırılması amaçlandı. Çalışma Van bölgesinde 2-3 yaşlı, 38 baş sağlıklı Akkaraman ırkı koyunda gerçekleştirildi. Ayrıca doğal aşım yaptırmak amacıyla 2 baş sağlıklı fertil koç kullanıldı. Yirmisekiz koyuna 18 mg melatonin içeren implantlardan birer tane kulak derisi altına uygulandı. On koyun kontrol olarak seçildi ve herhangi bir uygulama yapılmadı. Araştırmada kullanılacak iki koçun her birisine de aynı şekilde bu implantlardan üçer adet (toplam 54 mg melatonin) yerleştirildi. İmplantlar yerleştirildikten sonra sabah ve akşam olmak üzere günde iki kez koç taraması ile östrüs takibi yapılarak östrüste olanlar koçlara aştırıldı. Aşımların tamamlanmasından 45 gün sonra real time ultrasonografi ile gebelik muayeneleri yapıldı. Daha sonra da doğum kayıtları tutuldu. Melatonin ve kontrol grubuna ait tüm koyunlardan plazma progesteron seviyelerinin ölçümü için implant uygulama gününden itibaren haftada bir kez kan alındı. Plazma progesteron konsantrasyonları EIA yöntemiyle belirlendi. Erken anöstrüs döneminde melatonin ile östrüsler uyarılıp aşımlar yaptırıldığında % 82.14 gebelik elde edilirken, kontrol grubundaki koyunlarda normal üreme mevsimi gelinceye kadar hiç bir siklik aktivite ve gebelik sağlanamadı. İmplant uygulamasının başlangıcından ilk östrüslerin başlamasına kadar geçen süre melatonin grubunda 60.8±0.42 gün, kontrol grubunda ise 138.0±1.56 gün olarak bulundu. Sonuç olarak, Doğu Anadolu Bölgesinde yer alan Van bölgesinde Akkaraman ırkı koyunlara Mayıs ayı ortalarında melatonin implant uygulamasıyla östrüs ve ovulasyonların normal üreme sezonuna göre 2-2.5 ay erkene alınabileceği ve yüksek oranda gebelik elde edilebileceği kanısına varıldı
Koyunlarda Gebeliğin Çeşitli Dönemlerinde Serum Adenozin Deaminaz Düzeyleri
(2002) Altuğ, Nuri; Ağaoğlu, Zahit Tevfik; Alan, Muhammet; Uyar, Ahmet
-
Nobiletin Alleviates Methotrexate-Induced Hepatorenal Toxicity in Rats
(Taylor & Francis Ltd, 2024) Kazak, Filiz; Uyar, Ahmet; Coskun, Pinar; Yaman, Turan
We investigated the possible ameliorative effects of nobiletin (NBL) against methotrexate (MTX)-induced hepatorenal toxicity in rats. Twenty-eight Wistar albino rats were randomly divided into four groups, namely: Control; MTX (administered 20 mg/kg MTX); MTX+NBL (administered 20 mg/kg MTX and 10 mg/kg NBL per day); and NBL (administered 10 mg/kg/day NBL). Histopathological, immunohistochemical and biochemical analyses were performed on the kidney and liver tissues of rats at the end of the study. MTX caused renal toxicity, as indicated by increases in malondialdehyde (MDA) and caspase-3, as well as decreases in reduced glutathione (GSH), glucose-6-phosphate dehydrogenase (G6PD), glutathione peroxidase (GPx), catalase (CAT) and B-cell lymphoma-2 (Bcl-2). MTX also caused hepatotoxicity, as indicated by increases in 8-hydroxy-2'-deoxyguanosine (8-OHdG), tumor necrosis factor alpha (TNF-alpha), MDA and caspase-3 and decrease in interleukin 10 (IL-10), GSH, total antioxidant capacity, GPx, G6PD, CAT and Bcl-2. MTX caused histopathological changes in kidney and liver tissues indicating tissue and cellular damage. Administration of NBL concurrently with methotrexate reduced oxidative stress, inflammatory and apoptotic signs, and prevented kidney and liver damage caused by methotrexate. We consider NBL has attenuating and ameliorating effects on methotrexate-induced hepatorenal toxicity.
Preventative Effects of Glycrrhiza Glabra Tea on Formation Obesity by Feeding With High Fat Diet in Rats: an Investigation by Histopathological and Biochemical.
(2019) Özdere, Barış; Uyar, Ahmet; Yaman, Turan
ÖZDERE B. Ratlarda Yüksek Yağlı Diyet ile Obezite Oluşumu Üzerine Meyan Kökü (Glycrrhiza glabra) Ekstraktı Etkisinin Histopatolojik ve Biyokimyasal Olarak Araştırılması Yüzüncü Yıl Üniversitesi, Sağlık Bilimleri Enstitüsü, Patoloji Anabilim Dalı Yüksek Lisans Tezi, Van, 2019. Yapılan bu çalışmada yüksek yağlı diyet ile beslenen ratlarda, Meyan kökü (Glycrrhiza glabra)çayının obezite oluşumu üzerine etkisi histopatolojik ve biyokimyasal olarak araştırıldı. Bu maksatla 32 adet sağlıklı Wistar albino türü rat; Kontrol (K)(n:8), Yüksek Yağlı Diyet (YYD)(n:8), YYD+Meyan (YYD+M)(n:8) ve Meyan (M)(n:8) olmak üzere 4 gruba ayrıldı. Kontrol grubu ratlara standart pellet yem verildi. YYD ve YYD+M grubu ratlar 300g/kg oranında tereyağı eritilip yeme katılarak enerji değeri yüksek (4000 kcal/kg üzeri) pellet yemiyle 8 hafta süreyle beslendi. YYD+M ve M grubu ratlara 1gr/kg vücut ağırlığı olacak şekilde günlük hazırlanan Meyan kökü (Glycrrhiza glabra) çayı oral olarak verildi. Deney süresince periyodik aralıklarla ratların canlı ağırlık artışları izlendi. Histopatolojik olarak karaciğerden alınan doku örneklerinde YYD grubunda hepatositlerde makro ve mikroveziküler yağlanma, sinusoidal dilatasyon, hidropik dejenerasyon ve bazı hepatositlerde koagulasyon nekrozları görülürken, YYD+M grubunda tek tük makro ve mikroveziküler yağlanma, hafif derecede hidropik dejenerasyon ve nadiren koagulasyon nekrozları görüldü. Biyokimyasal analizlerde ALT, AST, ALP, LDH, Kolesterol, Trigliserit, HDL ve Kan Glukoz düzeyleri YYD grubunda kontrol grubuna göre belirgin düzeyde yüksek, YYD+M grubunda ise kontrol grubuna yakın olduğu tespit edildi. Sonuç olarak, YYD ile birlikte verilen Meyan Kökü (Glycrrhiza glabra) ekstresinin ratlarda obezite oluşumunun önüne geçebileceği kanatine varıldı.
Preventative Effects of Urtica Dioica Seed Extracts on Formation Obesity by Feeding With High Fat Diet in Rats: an Investigation by Histopathological and Biochemical
(2019) Kaçak, Kenan; Yaman, Turan; Uyar, Ahmet
Sunulan bu çalışmada, yüksek yağlı diyet (YYD) ile beslenen ratlarda ısırgan otu tohumu (Urtica Dioica; UD) ekstraktının obezite oluşumu üzerine etkisinin histopatolojik ve biyokimyasal olarak araştırılması amaçlandı. Bu amaçla 32 adet erkek Wistar albio rat her grupta 8 adet olmak üzere dört gruba ayrıldı. Kontrol grubu; standart rat pelet yem ile beslendi. YYD grubu; 300g/kg oranında tereyağı katılarak hazırlanan yüksek yağlı pelet yem ile beslendi. YYD + UD grubu; 300g/kg oranında tereyağı katılarak hazırlanan yüksek yağ diyetli pelet yem ile beslemeye ilaveten UD ekstresi (300 mg/kg, oral) ile beslendi. UD grubu; Standart pelet yem ile beslemeye ilaveten UD ekstresi (300 mg/kg, oral) ile beslendi. 75 günlük deneme süresi sonunda sıçanların nekropsileri yapılarak doku ve kan örnekleri alındı. Alınan doku örnekleri Hematoksilen&Eozinle(H & E) ile boyanarak histopatolojik inceleme yapıldı. Alınan kan örneklerinden AST, ALT, LDH, ALP, trigliserid, total kolesterol, HDL-kolesterol, LDL-kolesterol ve açlık kan şekeri düzeyleri çalışıldı. Histopatolojik olarak YYD grubu ratların karaciğer dokularında hepatositlerde dejenerasyon ve nekroz ve remark kordon yaplarında bozulma görüldü. YYD + UD grubu ratlarda bu bulguların kısmen azaldığı belirlendi. YYD grubunda ALT, ALP, trigliserid ve LDL düzeyleri kontrol grubundan anlamlı olarak yüksek bulundu. YYD grubu ile karşılaştırıldığında, YYD + UD grubunda bu düzeylerin anlamlı olarak düştüğü belirlendi. YYD grubunda kontrole göre azalan HDL düzeyinin, YYD + UD grubunda YYD grubuna göre arttığı görüldü. YYD grubu ratlarda kolesterol düzeyinin kontrole göre arttığı belirlenirken, YYD + UD gurubunda kolesterol düzeyinin YYD grubuna göre daha yüksek olduğu tespit edildi. YYD ile beslenme kilo artşı sağlarken, UD eksratının uygulanması ile kilo alımında kısmen azalma meydana geldi. Sonuç olarak, UD eksraktı uygulamasının karaciğerde meydana gelen tahribatı azalttığı, serum lipit düzeylerinde azalmaya neden olduğu ve kilo alımını kısmen engellediği, ancak kolesterol düzeyinde yükselmeye sebep olduğu belirlendiğinden, UD ekstresinin YYD ile beslenmenin olumsuz etkilerine karşı tam bir koruma sağladığı sonucuna kesin olarak varılamadı.