Browsing by Author "Uyar, H."

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The Effect of Chronic Exposure To Fluorine and 7,12-Dimethylbenz(a)anthracene on Anxiety, Locomotor Activity, Spatial Learning, and Memory Consolidation in Rats
(int Soc Fluoride Research, 2020) Uyar, H.; Oto, G.
In this study, the effect of fluorine, which is known to cause chronic toxication, and 7,12-dimethylbenz(a)nthracene (DMBA) on anxiety, locomotor activity, spatial learning, and memory consolidation in rats were investigated. Ninety adult Wistar albino male rats, weighing 150-200 g, were divided into 9 groups of 10 animals and treated for 90 days with fluoride (F), the ion of fluorine, in drinking water in the form of sodium fluoride (NaF) and weekly oral DMBA (10 mg/kg bw/po/weekly) in a sesame oil vehicle, in the following manner: group 1: 0 ppm F, 0 mg/kg bw DMBA (control); group 2: sesame oil weekly (vehicle control); group 3: 1 ppm F; group 4: 15 ppm F; group 5: 30 ppm F; group 6: DMBA; group 7: 1 ppm F and DMBA; group 8: 15 ppm F and DMBA; and group 9: 30 ppm F and DMBA. Tests for anxiety (elevated plus maze), locomotor activity (rotarod), and spatial learning and learning consolidation (Morris water maze) were administered to rats at the beginning and at the 30th, 60th, and 90th days of the study. The results showed that anxiety was increased by F at a low dose and DMBA, and decreased by high dose F. Locomotor activity was increased by F and decreased by F+DMBA. DMBA could either increase or decrease motor activity depending on the time of assessment. Spatial learning and memory consolidation were decreased by both F and DMBA. After considering these results, we plan to conduct further studies to clarify the mechanisms underlying these neurotoxic effects of fluorine and DMBA.
Effects of Chronically Exposure To Flor and 7,12-Dimethylbenzanthracene (Dmba) on Spermatogenesis and Testicular Histopathology in Rats
(Ataturk Universitesi, 2018) Yildirim, S.; Belhan, S.; Uyar, H.; Huyut, Z.; Oto, G.; Sağlam, Y.S.
This study aimed to investigate the effects of both sodium fluoride (NaF) and polycyclic aromatic hydrocarbon (PAH) derivative such as 7,12-dimethylbenz[a]anthracene (DMBA) singly and in combination on spermatogenesis and testis histopathology in rats. Forty male Wistar albino rats weighing 250–300 g used. On the 90th day of the experiment, intracardiac blood samples and testes were taken. While there was no change in FSH and LH levels in the groups, testosterone levels were significantly decreased in the other groups according to the control group (P<0.001). It was decreased the ratio of motility of sperm, sperm density and, increased the abnormal sperm rate by chronic fluorosis and DMBA toxicity in rats significantly. In histopathological examinations; A decrease in the number of spermatocytes and spermatozoa in the tubulus wall of the testes of rats in the NaF (30 ppm) group was observed. When testicular tissues of rats in the DMBA (10 mg/kg) group were examined, atrophy in tubulus, edema in intertubular spaces and a decrease in the number of spermatocytes in the tubulus wall were found. When testes tissues of rats in the group of NaF (30 ppm) + DMBA (10 mg/kg) were examined, atrophy in seminiferous tubules, severe degenerative and necrotic changes in Spermatogoniums on the walls of tubules and thinning in tubule walls were observed. There is not found spermatozoon in the tubul lumens. Severe edema in the intertubular spaces, dilatation and hyperemia in the veins found in these regions. As a result, it was observed that NaF and DMBA had a negative effect on testicular tissue and fertility singly, and in combination, this effect was further exacerbated. © 2018 Ataturk Universitesi.All Rights Reserved.
How Long-Term Intake of Sodium Fluoride (Naf) in Different Doses and 7,12 Dimethylbenz(A)anhtracene (Dmba) Affect the Erythrocyte Parameters in Rats
(Pakistan Agricultural Scientists Forum, 2019) Comba, B.; Oto, G.; Arihan, O.; Comba, A.; Uyar, H.
This study was aimed to search the effect of Sodium Fluoride (NaF) and 7,12 dimethylbenz(a)anthracene (DMBA) to erythrocyte fragility and parameters in rats. The nine groups were formed and each group contained 8 animals. Group 1: Control group (without any treatment). Group 2: Sesame oil (vehicle for DMBA). Group 3: 1 ppm NaF. Group 4: 15 ppm NaF. Group 5: 30 ppm NaF. Group 6: DMBA.Group 7: 1 ppm NaF + DMBA. Group 8: 15 ppm NaF + DMBA. Group 9: 30 ppm NaF + DMBA. Fluoride was added into the animals' drinking water in the form of NaF once a day, for 12 weeks. DMBA (10 mg/kg) was administered once a week and in a total of 12 weeks with oral gavage. Erythrocyte fragility was analyzed with osmotic hemolysis method and erythrocyte parameters with blood cell counter in whole blood. At 0.4% NaCl concentration groups 4, 5, 6, 8, and 9 showed significantly higher erythrocyte fragility values than control group (p <= 0.05). At 0.5% NaCl concentration, groups 6, 8 and 9 showed significant increase in erythrocyte fragility compared to other groups (p <= 0.05). The erythrocyte and hematocrit values were found significantly high in group 5 (p<0.001) and group 4 (p<0.01) while it was found low in all groups with DMBA (6, 7, 8, 9) (p <= 0.05) compared to control group. Hemoglobin amount in group 5(p <= 0.01) and group 4 (p <= 0.05) were significantly higher than other groups. MCV and MCH in group 5 were significantly lower and these values in all groups with DMBA (6, 7, 8, 9) were determined significantly high compared to other groups. RDWC in group 5 (p <= 0.001) and group 4 (p <= 0.01) and in all groups with DMBA (6, 7, 8, 9) (p <= 0.05) was significantly increased compared to other groups. As a result, exposure to high doses of floride and DMBA may cause augmented erythrocyte fragility, abnormal erythrocyte parameters and anemia. Therefore, measures must be taken to protect the health of all living organisms in area exposed to high levels of fluoride and DMBA.
The Protective Role of Resveratrol on Serum Total Sialic Acid and Lipid-Bound Sialic Acid in Female Rats With Chronic Fluorosis
(Yuzuncu Yil Universitesi Tip Fakultesi, 2016) Oto, G.; Ekin, S.; Özdemir, H.; Bulduk, M.; Uyar, H.; Öksüz, E.
In the present study, the effect of resveratrol on serum total sialic acid (TSA) and lipid bound sialic acid (LSA) was investigated in the rats exposed to chronic fluoride. The study was administered using 32 female Sprague Dawley rats weighing 200-250 g. Rats were divided into four groups (n=8/group). Group I comprised the control group, group II was treated with sodium fluoride (NaF) (10 mg/L/day), group III was treated with resveratrol (50 mg/L/day) and group IV was treated with NaF+resveratrol for 90 days period. Total sialic acid (TSA) and lipid-bound sialic acid (LSA) were determined in serum samples. Statistical analysis showed that the NaF group was significantly higher than the control group with regards to LSA (17.59±2.734 mg/dL, 12.61±2.013 mg/dL) and TSA (87.86± 8.34 mg/dL, 71.47± 8.57 mg/dL) levels (p<0.01 and p<0.05 respectively). Whereas the Resveratrol group was also significantly lower than the NaF group regarding LSA (13.21±2.848 mg/dL, 17.59±2.734 mg/dL) and TSA (72.44± 10.43 mg/dL, 87.86± 8.34 mg/dL) levels (p<0.05 and p<0.05 respectively), Moreover, no significant differences in LSA (14.62±1.85 mg/dL, 12.61±2.013 mg/dL) and TSA (81.19 ±10.24 mg/dL, 71.47± 8.57 mg/dL) levels were observed in the Resveratrol + NaF groups, as compared to the control group (p>0.05). The present study demonstrated slight positive and beneficial effect of resveratrol on the concentration levels of LSA and TSA in serum. © 2017 Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.