Browsing by Author "Uyar, Hasan"

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The Effect of Boric Acid and Borax on Blood Pressure in the Benzo(A)pyrene (B and 3-Methylcholanthrene (3-Mc) Applied Rats
(Wiley-blackwell, 2015) Bulduk, Bahattin; Oto, Gokhan; Ozdemir, Hulya; Gunbatar, Nizamettin; Uyar, Hasan; Bulduk, Mehmet; Elaslan, Sadi
Effect of Chronic Exposure To Sodium Fluoride and 7,12-Dimethylbenz[a]anthracene on Some Blood Parameters and Hepatic, Renal, and Cardiac Histopathology in Rats
(int Soc Fluoride Research, 2018) Yildirim, Serkan; Ekin, Suat; Huyut, Zubeyir; Oto, Gokhan; Comba, Arzu; Uyar, Hasan; Cinar, D. Ali
This study aimed to investigate the effects of both sodium fluoride (NaF) and 7,12-dimethylbenz[a]anthracene (DMBA), both separately and in combination, on some blood parameters and hepatic, renal, and cardiac histopathology in rats. Forty male Wistar albino rats, weighing 250-300 g, were randomly divided into one control and three experimental groups (i) a NaF group who received 15 ppm of NaF in their drinking water for 90 days, (ii) a DMBA group who received 10 mg DMBA/kg body weight/po/ weekly for 90 days, and (iii) a NaF+DMBA group who received 15 ppm NaF in their drinking water plus 10 mg DMBA/kg bw/po/weekly for 90 days. The animals in the groups were sacrificed at the end of the 90 days. The AST, ALT, LDH, CK, creatinine, troponin I, and MDA levels increased in the NaF, DMBA, and NaF+DMBA groups compared to the control group, while the WBC, K, Na, Cl, urea, SOD, GSH-Px, CAT, and GSH values showed a statistically significant decrease (p<0.05). In addition, the CK-MB significantly increased in the DMBA and NaF+DMBA groups compared to the control group (p<0.05). The histological structure of the liver, kidney, and heart tissues in the control group was normal. In the NaF and DMBA groups, degenerative and necrotic changes were detected. In the NaF+DMBA group: (i) the liver exhibited hydropic degeneration and coagulation necrosis in hepatocytes, severe dilation in the sinusoids, congestion in the central and portal regions, and mononuclear cell infiltration in the portal region; (ii) the kidneys displayed congestion in the glomerulus and interstitial vessels, interstitial nephritis, diffuse hydropic degeneration, and coagulation necrosis in the tubule epithelium; (iii) the heart showed myocardial hyperemia, severe mononuclear cell infiltration in interstitial tissue, hyaline degeneration, and Zenker's necrosis in myocardium As a result of these blood and oxidative stress parameters and histopathological findings, it was determined that NaF, DMBA, and NaF+DMBA induce toxicity in the liver, kidney, and heart tissues and thus play an important role in the physiopathology of toxicity.
Effect of Exposure To Fluorine and 7, 12-Dimethyl Benzanthracene on Vascular Responses
(Parlar Scientific Publications (p S P), 2022) Bulduk, Bahattin; Uyar, Hasan; Oto, Gokhan; Ozdemir, Hulya; Gunbatar, Nizamettin; Bulduk, Mehmet; Elasan, Sadi
In this study, the effect of experimental administration of fluorine and DMBA, alone or in combination, on rats on vascular responses was investigated. After adaptation, the rats were randomly selected and divided into 9 groups, 10 male rats in each group. While sodium fluoride was applied daily in drinking water for 12 weeks to the groups to which fluoride will be applied; on the other hand, DMBA was dissolved in sesame oil and applied to the groups that needed it, once a week for a total of 12 weeks. The groups were determined as C, SO, 1NaF, 15NaF, 30NaF, DMBA, 1NaF+DMBA, 15NaF+DMBA and 30NaF+DMBA. Tensions in the thoracic aorta were provided with phenylephrine. Then, relaxation responses were recorded by giving ACh ((10-8'-7'-6'-5)) and SNP ((10-,-9,-8,-7,)(-6)(,)(-5)) at different cumulative concentrations. In the study, it was determined that fluorine caused the vessels to contract and DMBA to relax. Concurrent exposure to high amounts of fluorine and DMBA was observed to cause vasoconstriction.
Effects of Thymus Vulgaris L. in Acute and Chronic Epilepsy Models in Rats Induced by Pentylenetetrazole
(Kafkas Univ, veteriner Fakultesi dergisi, 2019) Ozdemir, Hulya; Sagmanligil, Vedat; Erkec, Ozlem Ergul; Oto, Gokhan; Basbugan, Yildiray; Uyar, Hasan
The aim of this study was to investigate the effects of Thymus vulgaris L. (TVL) on oxidative stress, motor coordination and learning/memory in acute and chronic epilepsy models in rats induced by Pentylenetetrazole (PTZ). To this end, 64 male Wistar-albino rats were randomly divided into eight groups with 8 rats each: (1) acute control (AC), (2) acute PTZ (APTZ), (3) acute PTZ + sodium valproate (APTZ+VPA), (4) acute PTZ + TVL (APTZ+TVL), (5) chronic control (CC), (6) PTZ kindling (PTZk), (7) PTZ kindling + VPA (PTZk+VPA) and (8) PTZ kindling + TVL (PTZk+TVL). Seizures were observed for 30 min after each PTZ injection and were scored. Acute PTZ-induced seizures were created by injecting a single convulsive dose of PTZ (60 mg/kg, ip) in acute groups. PTZ kindling was produced by injecting a subconvulsant dose of PTZ (35 mg/kg, ip) every other day, with 14 injections in total. No significant difference was found among the PTZk + VPA, PTZk, and PTZk + TVL groups with regard to seizure scores. No significant difference was found among all the 8 groups in the learning/memory tests conducted using the Morris Water Maze (MWM) test and the motor activity tests conducted using the rotarod test (P>0.05). The analysis of total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) indicated that the administration of PTZ decreased the antioxidant capacity and increased the oxidant capacity. Moreover, the TVL administration established the oxidant/antioxidant balance, particularly in the chronic groups. Further studies are needed to investigate whether high doses of TVL have an effect on PTZ-induced seizure scores.
The Evaluation of Serum Total Sialic Acid and Lipid-Bound Sialic Acid Levels in Chronically Exposed Rats To 7,12-Dimethylbenz(a)anthracene and Fluoride
(Amer inst Physics, 2017) Oto, Gokhan; Ekin, Suat; Uyar, Hasan; Ozdemir, Hulya; Yildiz, Damla; Karakus, Yagmur
In this study, changes in serum total sialic acid (TSA) and lipid-bound sialic acid (LSA) levels were examined in chronically exposed rats to 7,12-dimethylbenz(a)anthracene (DMBA) and fluoride. This study demonstrated that the TSA, LSA levels increased more in DMBA-treated groups compared to the fluoride treated groups. The result obtained has shown that the harmful effect of DMBA which is also causing more cell membrane damage on human and animal health should be taken into consideration.
How Long-Term Intake of Sodium Fluoride (Naf) in Different Doses and 7,12 Dimetilbenz(A)antrasen (Dmba) Affect Erythrocyte Fragility and Parameters in Rats
(Wiley, 2017) Comba, Bahat; Oto, Gokhan; Arihan, Okan; Comba, Arzu; Uyar, Hasan