Browsing by Author "Uzal, Ozlem"

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Antioxidant Enzyme Activities and Lipidperoxidation Amount of Pea Varieties (Pisum Sativum Sp Arvense L.) Under Salt Stress
(Parlar Scientific Publications (p S P), 2016) Yasar, Fikret; Uzal, Ozlem; Yasar, Ozlem
This study examined the responses to salt stress of 5 pea genotypes, B-6, 110121, 1103220, 101917 and 10431 and two pea cultivars Winner and Karina under 75 mM NaCl salt treatment. Pea genotypes were selected from among cross-breed varieties of wild pea and white flowered pea growing different regions of Anatolia. While salinity reduced significantly fresh weight of BA, 110121, it did not cause a significant change fresh weight of the other pea varieties used in this study as compared to the control. Lipid peroxidation was increased by NaCl treatment in all varieties. This increase was more pronounced in BA and 11121 relatively to other pea varieties. On the basis of plant growth and MDA contents, B-6 and 110121 appeared as being more salt sensitive than other varieties When compared to control, salt treatment increased antioxidant enzyme activities in all pea varieties used in this study. The increases were greater in salt tolerant 1103220, 101917 and 10431 pea varieties than salt sensitive BA and 110121 varieties. These results suggest that pea seedlings respond to salt-induced oxidative stress by increasing their antioxidant defense systems.
Changes of the Lipid Peroxidation and Chlorophyll Amount of Green Bean Genotypes Under Drought Stress
(Academic Journals, 2010) Yasar, Fikret; Uzal, Ozlem; Ozpay, Taylan
One of the environmental stresses, and maybe one of these important drought stresses, cause deterioration of oxidative on the plant cell. Beans is one of the vegetables influenced by high level of drought. Drought stress deterioration effects on beans and its aim determines these effects either as been different or not caused by drought stress dependent on 10 item beans (Phaseolus vulgaris L.) variety. To get the hydroponic condition ready in time within 15 days, plant with 10% ratio of polyethyleen glycol (PEG 6000) were used to create drought effects. Bean leaves were harvested 6 days later after PEG implementation and were measured. The amount of lipid peroxidation production and malondyhalheit were directed towards determining the oxidative deterioration and in addition the chlorophyll amount. As a result of drought implementation, Samsun 96 (S96) and Sirik Barbunya (SB) beans genotypes have the most decreased chlorophyll amount than Gevas Sirik 57(GS57) and Oturak Barbunya (OB) genotypes. At the same time, S96 and SB genotypes have the most increased malondyhalheit (MDA) content than GS57 and OB. Also, of the total ten item of beans variety, Gevas Sirik 57 and Oturak Barbunya were least affected by drought stress, while S96 and SB genotypes showed more sensitivity to drought stress.
The Developmental and Metabolic Effects of Different Magnesium Dozes in Pepper Plants Under Salt Stress
(Univ Agr Sci & veterinary Med Cluj-napoca, 2020) Zirek, Neslihan Sevgin; Uzal, Ozlem
Morphological and biochemical effects of different magnesium (Mg) doses on pepper plants under salt stress were investigated in this study. Experiments were conducted under controlled conditions of a climate cabin at 25 degrees C temperature, 70% relative humidity and 16/8 hours light/dark photoperiod. The developmental and metabolic effects of different magnesium doses in plants under salt stress were investigated by examining leaf antioxidant enzyme activities, Malondialdehyde (MDA) levels and chlorophyll contents. Seedlings of 'Demre' pepper cultivar (Capsicum annuum L. cv. 'Demre') were grown in Hoagland nutrient solution supplemented with 100 mM NaCl to generate salt stress. Besides salt treatments, different Mg doses (Mg 1 = 24.64 ppm, Mg 2 = 49.28 ppm, Mg 3 = 73.92 ppm, Mg 4 = 98.56 ppm, Mg 5 = 123.20 ppm) were applied to plants. On the 20th day of salt treatments, the total weight of the plants which is one of the growths and development parameters of pepper plants was measured, and plant samples were taken for analyses. A slight increase was observed in total weights of salt-treated plants with increasing Mg doses. The greatest plant weight was obtained from Mg 4 + salt treatments. It was observed that increasing Mg doses had positive effects on the development of plants under salt stress. Chlorophyll contents and antioxidant enzymes activities increased and MDA (malondialdehyde) levels, the product of lipid peroxidation, which indicates the amount of damage to plant cells, decreased with increasing Mg doses. Present measurements and analyses and resultant findings revealed that Mg treatments at increasing doses partially alleviated negative effects of salt stress on pepper seedlings.
Effect of Applications of Different Potassium (K+) Doses on Antioxidant Enzyme Activities in Pepper Plants Under Salt Stress
(Polish Society Magnesium Research, 2021) Yasar, Fikret; Uzal, Ozlem
This study was carried out to determine the antioxidant enzyme activities in pepper (Capsicum annuum L. cv. Demre - long pepper) plants grown under salt stress and supplied different doses of potassium (K+). The study was conducted under controlled conditions in a 16/8 h light / dark photo period, at 25 degrees C and in a chamber with 70% humidity. After the seeds planted in pumice were germinated, seedlings with 2 true leaves were transferred into hydroponic culture containing the Hoagland nutrient solution. The K+ concentrations in the Hoagland solution were calculated as 146 mg L-1, and this level was used as a control. Other doses were set to be 20 mg L-1 lower and then 20 mg L-1 and 40 mg L-1 higher than the control dose (K1 = 126 mg L-1, K2 = 146 mg L-1, K3 = 166 mg L-1, K4 = 186 mg L-1). The salt stress level applied to the plants was induced by 100 mM sodium chloride (NaCl). Sampling for measurement and analysis was done on the 20th day of the salt application. The samples underwent the following determinations: Catalase (CAT), Ascorbate Peroxidase (APX) and Superoxide dismutase (SOD) relative to the total plant weight, and Antioxidative enzyme activities. The results indicate that K+ applications in the K3 = 166 mg L-1 and K4 = 186 mg L-1 doses reduced the impact of salt stress and the plants were less severely affected. Their antioxidant enzyme activities values in comparison to the control and other doses of K+ were quite low.
The Effect of Ga3 Applications at Different Doses on Lipidperoxidation, Chlorophyll, and Antioxidant Enzyme Activities in Pepper Plants Under Salt Stress
(Parlar Scientific Publications (p S P), 2017) Uzal, Ozlem
The developmental and metabolic effects of gibberellic acid application in plants under salt stress was investigated by examining leaf antioxidant enzyme activities, MDA levels, and chlorophyll content. Pepper seedlings (Capsicum annuum cv. Demre) grown in water culture under controlled conditions were subjected to salt stress (100 mM NaCl) and/or GA(3) treatment (0, 5, 7.5, and 10 ppm). The activities of antioxidant defense system enzymes (superoxide dismutase, catalase, and ascorbate peroxidase) and the chlorophyll and malondialdehyde (MDA) contents were determined in leaves harvested on the 20th day of the experiment. GA(3) was applied to plants by adding GA(3) to the Hoagland nutrient solution. Salt and different doses of GA(3) applications to the pepper plants showed that the GA(3) doses at 5 and 7.5 ppm applied with salt partially inhibited plant growth and development and thus, protected plants against the oxidative stress caused by salt. The leaf antioxidant enzyme activities, MDA accumulation and chlorophyll content of GA(3) and salt-applied plants were considerably low compared to the salt only application. In the GA(3)-only application, the results were similar to those obtained in the control group (no salt, no GA(3)), except for the chlorophyll content. In the salt-only application, plant root and leaf weight, plant length and internode lengths were lower than those of the control group (no salt, no GA(3)), while the antioxidant enzyme activities and MDA and chlorophyll contents were higher than those of the control group (no salt, no GA(3)). In summary, GA(3) application to pepper plants partially suppressed the adverse effects of salt stress on plant development and metabolic activities. Therefore, GA(3) may provide direct or indirect protection against stress.
Effect of Some Organic Materials on Yield and Plant Nutrient Content of Curly Leaf Salad (Lactuca Sativa Var. Crispa)
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2021) Tuga, Halide; Uzal, Ozlem; Yasar, Fikret
Caipira (Lactuca sativa var. Crispa) curly leaf salad varieties used in the study and 3 different organic materials (vermicompost, gidya, leonardit) and their control group using different doses (3%, 6%, 9%) and garden soil (0%) designed as. In this study, it is aimed to determine the effects of vermicompost, gitya and leonardite applications on plant nutrient content in different doses as organic material in salads that have an important place in human nutrition and when chemical nitrogen fertilizers are applied. According to the research findings, the effects of organic substances applied on the yield components examined were found to be significant. Although vermicompost application increased the yield approximately 2 times, no statistical difference was found between the doses. It was also determined that the effect of vermicompost on early growth in curly leaf salad was statistically significant and vermicompost gave good results especially to the intake of K+, Zn+ and Cu+ elements into the plant. In general, it has been determined that applications of gidya in terms of plant nutrient content do not give positive results.
Effects of Different Doses of Exogenous Gibberellic Acid on Total Plant Weight, Lipid Peroxidation, and Antioxidant Enzyme Activities of Eggplant Seedling Under Salt Stress
(Parlar Scientific Publications (p S P), 2019) Uzal, Ozlem; Yasar, Fikret; Yasar, Ozlem
The present study investigated the effects of different doses of gibberellic acid (GA(3)) on Aydin siyahi eggplant variety exposed to salt stress. The study was conducted to determine how applications affect plant development and metabolic activity. Eggplant seedlings were grown in a temperature-controlled hydroponic system in Hoagland nutrient solution. When they were 3 weeks old, they were exposed to salt stress (100 mM NaCl). Subsequently, they were treated with different doses of GA(3) (2.5, 5.0, 7.5, and 10 ppm). Leaves were harvested on the 15th day of the experiment. Fifteen days after the induction of salt stress, physiological (total plant weight) and biochemical (antioxidant enzyme activity, malondialdehyde [MDA] content, and chlorophyll content) changes were measured to determine the effect of the GA(3) treatments on plant growth and metabolic activity. In the NaCl plus high GA(3) treatment, the plant chlorosis level and degree of salt-induced damage decreased. In the NaCl plus high GA(3) (10 ppm treatment), plant development slowed due to salt-induced stress, but symptoms of stress, such as curling or chlorosis, were not detected in plant leaves. All doses the GA(3) treatments reduced antioxidant enzyme activities and MDA contents. In summary, the application of GA(3) to eggplant partially suppressed the adverse effects of salt stress on plant development and metabolic activities, and this effect was more apparent at a GA(3) dose of 10 ppm. Therefore, GA(3) may provide direct and indirect protection against salt stress.
Effects of External Melatonin Treatments on Morphological and Physiological Changes in Cucumber (Cucumis Sativus L.) Seedlings Against Chilling Stress
(Springer, 2023) Uzal, Ozlem; Baslak, Lutfullah; Yasar, Fikret
This study aimed to determine chill resistance and to explain how chilling stress affects metabolic processes by applying different doses of melatonin to Beith F1 hybrid cucumber (Cucumis sativus L.). In addition, experiments were conducted to understand the response of plants with and without melatonin to chilling stress and their adaptation mechanisms against chilling. Melatonin-containing (0, 1, 10, 20, 30 and 40 mu M) distilled water was sprayed to the leaves of chilling-treated seedlings with 3-4 true leaves. One full day after melatonin treatments, half of the plants were exposed to chilling stress in a climate chamber for 15 days and the other half was kept in the climate chamber under normal conditions (25/20 degrees C, 12/12 light/dark photoperiod). Plants exposed to chilling stress were kept in an incubator for 15 days at 5 +/- 1 degrees C dark (12 h)/10 +/- 1 degrees C light (12 h), then samples were taken. Some plant growth parameters were measured, and some biochemical analyses were performed. In terms of plant total fresh weights, it was observed that chilling stress and plants treated with 30-40 mu M melatonin showed limited plant growth and development and thus were less influenced by chilling stress. Melatonin treatments slowed down the growth rate of plants under cold stress, but, as it increased antioxidant enzyme activities, it protected plants from the destructive effect of stress and reduced the formation of malondialdehyde, a product of lipid peroxidation. In addition, there was no decrease in the amount of chlorophyll of the plants since malondialdehyde prevents the degradation of chlorophyll pigments. It was concluded based on present findings that external melatonin treatments could be used as a physiologically effective auxiliary application to reduce harmful effects of chilling stress.
Effects of Proline Treatments on Plant Growth, Lipid Peroxidation and Antioxidant Enzyme Activities of Tomato (Solanum Lycopersicum L.) Seedlings Under Chilling Stress
(Springer, 2022) Uzal, Ozlem
Experiments were conducted in a climate room with split air conditioner to determine the effects of foliar proline treatments on chilling stress-tolerance of Bandita F1 tomato (Solanum lycopersicum L.) seedlings through some morphological and physiological parameters. Chilling treatments were initiated when the seedlings had 3-4 true leaves. Seedlings to be chilled were sprayed with distilled water containing 0, 5, 10 and 15 mM proline. Seedlings were exposed to chilling stress in an incubator for 7 days and untreated (control) seedlings were kept in a climate chamber under normal conditions (25/20 degrees C day/night). Total weight of green parts, MDA (malondialdehyde) content and antioxidative enzyme activities (catalase (CAT), ascorbate peroxidase (APX), superoxide dismutase (SOD)) were determined on sample leaves. Stress-induced damages were assessed using a 1-5 scale. It was determined that foliar applications of proline to plants subjected to chilling stress had a positive effect on the development and damage of the plant. It was determined that there was a positive relationship between the scale values and the MDA amounts, and the MDA levels of the application with a high scale value were also high. Likewise, under the application of 10 mM proline, which had a low scale value, the plants were less affected by chilling stress and the amount of MDA was observed to be low. Since the antioxidative enzyme activities were at the highest level in the 10 mM proline application, the plants were found to be affected by the cold stress at the lowest level. It was observed that 10 mM foliar proline application had the most positive effect on all parameters measured in tomato plants and relatively protected the plants against chilling stress.
Effects of Two Different Light Intensities on Plant Growth, Ion Uptake and Distribution in Tomato Plants
(Polish Society Magnesium Research, 2022) Yasar, Fikret; Akin, Seyfettin; Uzal, Ozlem
This study was carried out to determine relationships between light intensity and nutrient elements by examining the growth parameters, ion uptake and distribution between plant organs of tomato plants under controlled conditions. In the study, seedlings of an indeterminate Adamset F1 hybrid tomato variety were used. The seedlings were grown by applying two different light intensities: 400 mu mol m(-1) s(-1), which is the optimum light intensity for tomatoes, and 800 mu mol m(-1) s(-1), which is twice the optimum light intensity. In this study, LED lamps, which emit light close to sunlight, were used as the light source. Sampling for measurement and analysis was done on the 40(th) day of the application. The Hoagland nutrient solution was replenished every week. Leaf weight, leaf number, stem weight, stem length, internode distance, stem thickness and root weight of the plants taken on the 40th day were measured. Concentrations of macro- and micronutrients (N, P, K, Ca, Mg, Fe, Mn, Zn, Cu) in the root, stem and leaf samples of plants were also measured. The root, stem and leaf weights of the plants that were treated with two different light intensities were found to be different. The highest root, stem and leaf weights were observed in plants with high light exposure. It has been found that light intensity is effective in stimulating the ion uptake and distribution, and especially the amount of microelements in all three organs of tomato plants analyzed, and there was higher uptake under high light application compared to optimum light application.
Enzyme Activities of Certain Pumpkin (Cucurbita Spp) Species Under Drought Stress
(Parlar Scientific Publications (p S P), 2014) Yasar, Fikret; Uzal, Ozlem; Kose, Serif; Yasar, Ozlem; Ellialtioglu, Sebnem
The aim of this study is to investigate the relationship between the drought tolerance capacity and antioxidant enzyme activity of 38 genotypes of different pumpkin species from different regions of Turkey. The seedlings from 38 different genotypes were put into pots containing Hoagland's solution to be stored in cultivation rooms under controlled climatic conditions. A 15% polyethylene glycol 6000 (PEG 6000) solution was added to the Hoagland's solution to create an osmotic potential equivalent to -0.60 M=Pa for the drought test application. The leaf weights were determined following the test under drought and control conditions. The relative water content, chlorophyll content, and antioxidative enzyme activities (superoxide dismutase, catalase, and ascorbate perwddase) were investigated. As a result, it was observed that the enzyme activities are extremely vital in the drought tolerance of the pumpkin genotypes; such as under dry conditions, the drought tolerant pumpkin genotypes use antioxidative enzymes more actively compared to the drought susceptible genotypes.
Mitigation of Negative Impacts of Cadmium Stress on Physiological Parameters of Curly Lettuce (Lactuca Sativa Var. Crispa) by Proline Treatments
(Polish Society Magnesium Research, 2022) Altas, Serdar; Uzal, Ozlem
This study was conducted on a variety of lettuce curly (Lactuca sativa var. Crispa) called Caipira. The purpose was to elucidate the effects of cadmium stress on the plant???s metabolic processes, response to cadmium stress and adaptation mechanisms developed against cadmium stress with the use of proline treatments. Hydroponic experiments were conducted in a climate chamber supplied with a controlled atmosphere (15??2??C 13 h dark, 22??2??C 11 h light and 70% relative humidity). When the seedlings formed new roots and had 4-5 leaves, initially 5 mM, 10 mM, 15 mM proline solutions were sprayed on the leaves of a group of seedlings 4 days before applying cadmium stress, then the plants were exposed to 40 ppm cadmium. The other group was sprayed with 5 mM, 10 mM, 15 mM proline solutions just before applying cadmium stress. Yet another group of plants was subjected only to cadmium stress without proline treatments. Therefore, 8 different treatments were tested: Control (0 ppm cadmium), only cadmium (40 ppm), proline (5 mM, 10 mM, 15 mM) 4 days before the stress + cadmium, proline (5 mM, 10 mM, 15 mM) + cadmium stress together. The present findings revealed that 5 mM proline dose was relatively effective in mitigation of negative effects of Cd stress on plant physiological and metabolic processes. Proline treatments at this dose were found to be effective in alleviating the negative effects of Cd stress on growth parameters of curly lettuce plants. It was also concluded that such effects of external proline treatments varied with the treatment dose and timing.
Oxidative Stress and Antioxidant Enzyme Activities in Tomato (Solanum Lycopersicum) Plants Grown at Two Different Light Intensities
(Springer, 2023) Yasar, Fikret; Uzal, Ozlem
In the study, oxidative stress statuses and antioxidant enzyme activities caused by light were determined by applying two different light intensities to tomato plants under controlled conditions. The indeterminate type Adamset F1 hybrid tomato (Solanum lycopersicum) cultivar was used. It was grown in Hoagland nutrient solution in a controlled climate room with a light/dark photoperiod of 16/8 h, at 27 degrees C, 65% humidity. The seedlings were grown by applying two different lights of 400 mu mol m(-1) s(-1), which is the optimum light intensity for tomatoes, and 800 mu mol m(-1) s(-1), which is twice the optimum light intensity. In this study, LED lamps, which are close to sunlight, were used as the light source. Sampling for measurement and analysis was done on the 40th day of the application. The Hoagland nutrient solution was replenished every week. The total fresh plant weights (g) of the plants taken on the 40th day and the chlorophyll, malondialdehyde (MDA) amounts and antioxidative enzyme activities of the leaf samples taken from the middle part of the plants were examined. In addition, the amount of MDA and the activities of superoxide dismutase (SOD), catalase (CAT) and ascorbic peroxidase (APX), which are antioxidant enzymes found in the defense system of plants against stress, were determined in order to understand whether oxidative stress occurs. The total fresh weight, chlorophyll and MDA amounts of the plants grown at high light intensity were found to be higher than the optimum light intensity. It has been observed that there is an increase in antioxidative enzyme activities due to the low level of oxidative stress in plants grown at high light intensity. However, it is understood that the resulting stress is at a tolerable level within the antioxidative defense system.
Root, Stem, and Leaf Ion Accumulation in Drought-Stressed Green Bean (Phaseolus Vulgaris L.) Genotypes Treated With Peg-6000
(Parlar Scientific Publications (p S P), 2014) Yasar, Fikret; Uzal, Ozlem; Yasar, Ozlem; Ellialtioglu, S. Sebnem
The objective of this investigation was to determine the relationship between drought stress and the ion accumulation as well as distribution mechanisms of bean genotypes of the Phaseolus vulgaris L. species, namely, Gevas Bodur 64 (GB64), Samsun 100 (S100), Samsun 95 (S95), 4F-89F, Gevas Sink 57 (GS57), Gevas Sink 26 (GS26), Samsun 96 (S96), Sink Barbunya (SB), Kirkgunluk (KG), and Oturak Barbunya (OB), collected from various regions of Anatolia, Turkey. The seedlings of 10 different bean genotypes were cultivated in containers containing Hoagland's nutrient solution in a cultivation chamber, the climate conditions of which were kept under control. In order to generate drought stress, 10% (w/v) polyethylene glycol 6000 (PEG-6000), corresponding to an osmotic potential of -0.40 MPa, was added to the nutrient solution. After the application, the accumulation of potassium (K), calcium (Ca), iron (Fe), zinc (Zn), and manganese (Mn) ions in the roots, stems, and leaves of the plants was determined. There were differences among the genotypes in terms of ion uptake, ion accumulation, and ion distribution. It was concluded that plants protected themselves from negative effects of drought stress via balanced ion distribution among their organs.