Browsing by Author "Validov, Shamil"
Now showing 1 - 2 of 2
- Results Per Page
- Sort Options
Article The Effects of the L. Plantarum Strain Rc1.4 Starter Culture With or Without Sucrose Addition on Fermentation Efficacy, Microbial Content, and Aerobic Stability Indicators of Alfalfa Silage(Tubitak Scientific & Technological Research Council Turkey, 2021) Aksu, Taylan; Validov, Shamil; Karimova, Liliia; Demirel, Murat; Levendoglu, Taner; Erdogan, Sibel; Guney, MehtapTo develop the approach for efficient alfalfa silage fermentation, L. plantarum strain RC1.4 (LP-RC1.4) was used as starter culture with or without sucrose. Trial groups consisted of ten repetitions for each treatment including (1) control, with added sterile water (10 mL); (2) LP, silages treated with LP-RC1.4 alone (10(6) cfu g(-1) FM); (3) LP-S 1, silages treated with LP-RC1.4 (10(6) cfu g(-1) FM) plus sucrose at 10 g/kg fresh matter; (4) LP-S-2, silages treated with LP-RC1.4 (10(6) cfu g(-1) FM) plus sucrose at 20 g kg(-1) fresh matter. Inoculation improved the quality of the silaging demonstrating a lower decrease of silage dry matter (DM) in comparison to the control silage. The CO2 production was also lower in the inoculated silages. The addition of sucrose improved microbiological and biochemical parameters of the resulting alfalfa silage, simultaneously with the increased number of lactobacilli, raised the content of water-soluble carbohydrates, while the number of yeasts and molds decreased. It was concluded that LP-RC1.4 inoculation should be supported with sucrose to insure domination of LAB fermentation and enhance of the silage quality of alfalfa.Article Inhibitory Effects of Bacillus Licheniformis Wj53a and Homofermentative Lactic Acid Bacteria on Clostridial Growth in Corn Silage(Univ Agriculture, Fac veterinary Science, 2023) Afordoanyi, Daniel Mawuena; Diabankana, Roderic Gilles Claret; Krupin, Evgeny; Bikchantaev, Irek; Taylan, Aksu; Validov, ShamilThis study reports on the effectiveness of silage additive based on consortium Lacticaseibacillus paracasei L1a and Bacillus licheniformis WJ53A in comparison with three homofermentative lactic acid bacteria (LAB) (Pediococcus acidilactici 12c, L. Lacticaseibacillus paracasei L1a, and L. plantarum S1aR). For this purpose, the antagonistic activity of these isolated LAB was tested. The impact of the isolated LAB on silage acidity, organic acid content, and dry matter of silage was also determined. The fungal and bacterial DNA load in the conserved silages was estimated using quantitative PCR and their effects on the quality category of silage were evaluated. The obtained results showed all inoculants being effective in ensiling of maize with the consortium (L1a + WJ53A), showing a high lactic acid content (94.51%, P<0.0001) in comparison to control (88.28%, P<0.0001). The consortium also showed a lower acetic acid (5.49% to 10%, P=0.0002) and absolutely no butyric acid in respect to control silage (0% to 1.64%, P<0.0001). The qPCR results presented an increase in the final LAB DNA concentration of silage conserved with consortium (0.002487 ng, P=0.0002) when compared to control (0.001127 ng, P=0.0002) but did differ from the inoculant of L1a (0.001790 ng, P=0.0219). Also, the final fungal DNA concentration showed the effectiveness of the consortium by the reduction of fungal DNA in comparison to control (0.00027 ng against 0.00106 ng, P=0.0007). The most interesting result is the inhibition of clostridia growth based on the qPCR analysis which showed a lower clostridial DNA in silage inoculated with the consortium (P<0.001) against all the inoculants used in this study. This research shows the advantage of ensilaging with a consortium of a homofermentative LAB and a Bacillus strain B. licheniformis WJ53A which reduces the risk of clostridial infections in livestock farming.