Browsing by Author "Yaman, T."

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Ameliorative Effect of Astaxanthin on Ischemia-Reperfusion Injury of Skeletal Muscles
(Pakistan Agricultural Scientists Forum, 2022) Uyar, A.; Akkoyun, H. T.; Bengu, A. S.; Akkoyun, M. B.; Keles, O. f.; Atcali, T.; Yaman, T.
This experimental study aimed to investigate the ameliorative effect of astaxanthin (AST) on the prevention of skeletal muscle injury resulting from lower extremity ischemia/reperfusion (I/R). Twenty-eight (250-300g) male Wistar albino rats were divided into 4 groups as Control, I/R, I/R+AST and AST. In the control group, only anesthesia was induced for 2 h without I/R. In the I/R group, 2 h of reperfusion was facilitated following ischemia under anesthesia. For the I/R+AST group, 7 days prior to ischemia, 125 mg/kg AST was given through a gavage, and 2 h of ischemia and 2 h of reperfusion were facilitated under anesthesia. At the end of the study, blood and gastrocnemius muscle tissue samples were taken for biochemical, histopathological and immunohistochemical examinations. Compared to the control group, there were increased Malondialdehyde (MDA) levels and decreased Superoxide dismutase (SOD) and Catalase (CAT) enzyme activities in the I/R group (p??0.001). Degeneration, necrosis, inflammation, loss of striation, interfibrillar and interfascicular edema were seen in the histopathological examination of the skeletal muscles in the I/R group. These histopathological findings were minimal in the I/R+AST group. In the immunohistochemical examination of muscle tissue with the GPx1 primary antibody, a mild degree of GPx1 reactivity was observed in the I/R group, and a moderate degree of GPx1 reactivity was seen in the I/R+AST group. As a result, the strong ameliorative effect of AST on ischemia-reperfusion injury and its complications on skeletal muscles was demonstrated by biochemical, histopathological and immunohistochemical examinations.
Diagnosis of Q Fever and Brucellosis in Aborted Ovine Fetuses by Microbiological, Pathological and Immunohistochemical Methods
(Walter de Gruyter GmbH, 2018) Yesilmen, S.; Yaman, T.; Sağsöz, H.; Bademkiran, S.
Brucellosis and Q fever, two zoonoses, are important causes of abortion in ruminants, as well as economically significant diseases caused by a gram-negative bacterium. Determination of these diseases is therefore of great importance. In this study, the organs of 35 naturally infected and aborted ovine fetuses were examined for the presence of changes resulting from infections by Brucella melitensis and Coxiella burnetii, according to macroscopic, bacteriological, histopathological and immunohistochemical methods. B. melitensis was observed in 21 cases, and C. burnetii was observed in 8 cases of the aborted ovine fetuses, and these were determined with immunohistochemical methods. Brucellosis was observed in 18 of the aborted ovine fetuses, and this was determined by microbiological methods. Negative (-) results were found for all of the other fetuses. The Brucella antigen was determined to be localized as intracytoplasmic in mainly alveolar macrophages, bronchi, bronchioles, glandular epithelial cells around bronchial glands, neutrophils, hepatocytes and Kupffer cells. The Coxiella antigen was found to be localized in the alveolar macrophages in the lungs, bronchi, bronchioles and alveolus, and in the cytoplasms of bronchial gland epithelial cells, and in the cytoplasms of hepatocytes and Kupffer cells in the liver. Immunohistochemical and microbiological diagnoses of brucellosis and coxiellosis were compared; it was concluded that immunohistochemical methods were more safely applied than microbiological methods. © 2018 University of Sao Paolo. All rights reserved.
Epithelioid and Spindle-Cell Haemangioendothelioma in the Brain of a Dog: a Case Report
(Czech Academy of Agricultural Sciences, 2018) Yaman, T.; Uyar, A.; Keles, O.F.; Yener, Z.
A 9.5-year-old male Belgian malinois dog died after showing clinical symptoms that included fatigue, anorexia and dyspnoea. Necropsy revealed macroscopic findings in the brain and other organs. A solitary, brown-red-coloured mass, approximately 0.5 cm thick and 1.5 × 2 cm in diameter, was detected on the right side of the medulla oblongata, pons and cerebellum. The cut surface showed no invasion of the brain parenchyma. Histologically, the neoplasm was characterised by proliferation of endothelial cells, which showed epithelioid and spindle cell features. Some tumour cells had intracytoplasmic lumen formations containing red blood cells. The nuclei of the tumour cells were large and vesicular. In immunohistochemical experiments the tumour cells stained positive for factor VIII-related antigen, CD31 and CD34. A description is provided of the features of this epithelioid and spindle-cell haemangioendothelioma (EHE) that originated from vessels of the meninges in the subarachnoid space. © 2018, Czech Academy of Agricultural Sciences.
Exploring the Protective Effects of Chinar (Platanus Orientalis L.) Leaf on Testes Damage Induced by Ethanol in Rat Model
(Pakistan Agricultural Scientists Forum, 2024) Yaman, T.; Dogan, A.; Keles, O. F.; Donmez, F.; Battal, A.; Anuk, O. O.
The aim of this study was to investigate the protective effects of an infusion obtained from dried Chinar (Platanus orientalis L.; PO) leaves against ethanol (EtOH)-induced damage in rat testes. Thirty male Wistar rats were randomly and equally divided into 5 groups, as the Control, EtOH 20%, EtOH 20% + Silymarin (10 mg/kg/day), EtOH 20% + PO20 mg/mL infusion, and EtOH 20% + PO -60 mg/mL infusion. Histopathologically, treatment with PO -60 leaf infusion resulted in improvements in EtOH-induced damage in the testes by inhibiting the depletion of germ cells and loss of spermatozoa. Immunohistochemically, an increase in the expression of endothelial nitric oxide synthase (eNOS) and caspase-3 was observed in the EtOH group. Treatment with the PO extract markedly reduced the EtOH-induced expression of eNOS and caspase-3 in the EtOH 20% + PO -60 group. According to the biochemical results, both of the PO infusion treatments caused a decrease in the malondialdehyde levels compared to the EtOH group. On the other hand, the glutathione contents in the treatment groups were significantly higher than ethanol group. Fluctuations in antioxidant defense enzyme activities were determined. PO leaf might have a protective role against EtOH-induced testicular damage in rats by inducing apoptosis via the upregulation of eNOS expression and preventing lipid peroxidation.
Histopathological Findings of Foot-Rot Disease Which Causes Deaths in a Sheep Flock
(Universidade Federal do Rio Grande do Sul, 2017) Yaman, T.; Uyar, A.; Keles, O.F.; Yener, Z.
Background: Foot-rot is a contagious bacterial infection of the feet of sheep that causes lameness. This infection leads to major economic losses in wool, meat, and dairy industries throughout the world. Pathogenesis of foot-rot can be described as the damage of the interdigital skin, invasion of fecal bacterium Fusobacterium necrophorum, and finally the involvement of Dichelobacter nodosus in the infection. In the worst cases, the disease becomes widespread, and systemic bacterial infections may occur. The present study aims to describe macroscopic and histopathological findings of foot-rot lesions formed in the foot, heart, liver, and lungs to draw attention to these aspects of the disease. Case: The material of the study consisted of a 3-year-old sheep. The investigation was composed of the disease history; post-mortem examination; and histopathological analysis of lung, liver, heart, and foot lesions. At the anamnesis, it was reported that the diseased animal had lameness and weakness with other clinical symptoms, and approximately 10 infected sheep had died within a 1-week period. At necropsy, malodorous ulcerative lesions between the nails; focal-disseminated foci in the liver, lung, and heart; yellowish matte thickening in the heart valves; and hydropericardium were detected. After necropsy, tissue samples taken from the skin, lungs, spleen, kidneys, and liver were fixed in a 10% buffered formalin solution, paraffin embedded, sectioned, and stained with hematoxylin and eosin. Giemsa staining was done to detect the agents in the tissues. The slides were examined and photographed using a light microscope. Histopathological examination revealed that ulcerations between the nails were lesions shaped as the result of the foot rot disease. The focal-disseminated lesions in the internal organs were the foci of metastatic-embolic inflammation originating from the lesions between the nails. Rod-shaped bacteria were detected in foot, liver, and lung tissues by Giemsa staining. Discussion: Foot-rot has important economic and welfare impacts. A number of researches on the characterization of agents, regional incidence, pathogenesis, and macroscopic characterization and classification of lesions in the nail have been carried out on footrot disease. In the present case, the disease became systemic and numerous sheep died. Systemic infections are characterized embolic-metastatic inflammations formed in major vital organs such as the liver, lungs, brain, heart, and kidneys as the result of the vasculitis formed in the veins in the inflamed region, where the agents are spread by the bloodstream. Histopathologically, granulomatous foci were seen in the liver and lungs. In the middle of these foci were collapsed leukocytes and agents, and the surrounding area was composed of Langhans, foreign body giant cells, and mononuclear cells. Treatment with systemic infections is quite difficult. The disease can be treated, but the cost of treatment and care is very high. For this reason, foot-rot disease is among the most expensive sheep diseases requiring costly treatment. Because we believe foot-rot disease not only causes lameness but also leads to high mortality rates in sheep flocks, we presented the pathological findings to draw attention to these aspects of the disease. As far as we can investigate, no case reports have presented both macroscopic and microscopic findings of the disease that have formed in nails and internal organs. © 2017 Universidade Federal do Rio Grande do Sul. All rights reserved.
Mixture of Leaf and Flower Extract of Prunus Spinosa L. Alleviates Hyperglycemia and Oxidative Stress in Streptozotocin-Induced Diabetic Rats
(Elsevier B.V., 2021) Temiz, M.A.; Okumus, E.; Yaman, T.; Keles, O.F.
Diabetes Mellitus is a global health problem that leads to various complications associated with hyperglycemia. In traditional medicine, herbal treatment is one of the alternative ways to cope with this type of disease. The aim of this study is to investigate the antidiabetic and hepato-pancreatic protective effects of the mixture of Prunus spinosa leaves and flowers (PSE) extract in streptozocin-induced diabetes mellitus. Seven random experimental groups of Wistar rats (n = 8) were created as followed; control, diabetic, PSE25, PSE50, insulin, metformin, and acarbose. α-amylase and α-glucosidase inhibitory activities of PSE were determined. Antioxidant enzymes activities and lipid peroxidation were analyzed in the liver tissue. Histopathological examination of liver and pancreas was also performed. α-amylase and α-glucosidase IC50 inhibition values of PSE were found more efficient, comparing to those of standard acarbose. While blood glucose levels severely increased in all diabetic groups, PSE25 and PSE50 treatments were effective in regulating blood glucose levels. Moreover, administration of PSE25 and PSE50 improved insulin levels compared to the diabetic group. Although increased oxidative stress in the diabetes seriously suppressed antioxidant activities, PSE25 and PSE50 supplementation significantly recuperated liver antioxidant capacity. Despite severe degenerative and necrotic changes in diabetes, these findings alleviated with PSE administrations. Moreover, PSE treatments remarkably recuperated β-cells. These results reveal that there may be an alternative way to control high blood glucose levels, which is one of the most important complications of diabetes. Furthermore, PSE can provide a protection against oxidative stress, liver and pancreatic damage by augmenting antioxidant capacity in diabetes. © 2021 SAAB