Browsing by Author "Yasar, Fikret"

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Antioxidant Enzyme Activities and Lipidperoxidation Amount of Pea Varieties (Pisum Sativum Sp Arvense L.) Under Salt Stress
(Parlar Scientific Publications (p S P), 2016) Yasar, Fikret; Uzal, Ozlem; Yasar, Ozlem
This study examined the responses to salt stress of 5 pea genotypes, B-6, 110121, 1103220, 101917 and 10431 and two pea cultivars Winner and Karina under 75 mM NaCl salt treatment. Pea genotypes were selected from among cross-breed varieties of wild pea and white flowered pea growing different regions of Anatolia. While salinity reduced significantly fresh weight of BA, 110121, it did not cause a significant change fresh weight of the other pea varieties used in this study as compared to the control. Lipid peroxidation was increased by NaCl treatment in all varieties. This increase was more pronounced in BA and 11121 relatively to other pea varieties. On the basis of plant growth and MDA contents, B-6 and 110121 appeared as being more salt sensitive than other varieties When compared to control, salt treatment increased antioxidant enzyme activities in all pea varieties used in this study. The increases were greater in salt tolerant 1103220, 101917 and 10431 pea varieties than salt sensitive BA and 110121 varieties. These results suggest that pea seedlings respond to salt-induced oxidative stress by increasing their antioxidant defense systems.
Bazı Kavun (Cucumis Sp.) Genotiplerinin Tuz Stresine Tepkileri
(2007) Abak, Kazim; Kusvuran, Sebnem; Yasar, Fikret; Ellıaltıoglu, Seküre Sebnem
Öz: Arastırmada, kavunda tuza tolerans bakımından genotipler düzeyinde farklılıgın bulunup bulunmadıgını ortaya koymak; tuza toleransın belirlenmesinde bitki biyomas degerlerinin ve yapraklarda iyon biriktirme derecesinin kullanılma olanagını iincelemek amaçlanmıstır. Ayrıca tuz stresinde yapraklarda MDA miktarındaki artıs incelenmistir. 36 adet farklı genotip, tuza tolerans ve duyarlılık özelligi bakımından farklı parametrelere göre sıralanmıs, özellikler arasındaki korelasyon katsayıları belirlenmistir. Kavunda tuz zararının Na+ ve Cl- iyonlarının toksik etkisinden kaynaklandıgı, bu iyonları bünyede az bulunduran genotiplerde tuza toleransın daha yüksek oldugu gözlenmistir. Denemede kullanılan genotipler arasında Midyat, Besni ve Semame kavun genotipleri tuza tolerant olarak belirlenirken; Ananas ve Yuva çesitlerinin tuza en duyarlı kavun çesitleri oldugu ortaya konmustur.
Bazı Organik Materyallerin Karpuz (Citrillus Lanatus Thunb.)’ Un İyon Alımına Etkisi
(2020) Üzal, Özlem; Yasar, Fikret; Tuğa, Halide
Yürütülen çalışmada Crimson Sweet (Citrillus lanatus Thunb.) karpuz çeşidi kullanılmıştır. 3 farklı organik materyal (vermikompost, gidya, leonardit) vebunların farklı dozları (% 3, % 6, % 9) ile bahçe toprağının kullanıldığı kontrol grubunun, karpuzda iyon alımına etkisi incelenmiştir. Karpuz fideleri, her biri 3 kilogramlık hacme sahip olan saksılarda büyütülmüştür. Uygulamalar 5 tekerrürlü olup, her saksıda 1 adet bitki olacak şekilde toplam 50 adet karpuz fidesi kullanılmıştır. Çalışmanın sonunda bitki yapraklarından alınan örneklerde iyonmiktarları (N, K, Ca, Mg, Cu, Fe, Zn, Mn), içeriğine bakılmıştır. Yapılan analiz sonunda gerek uygulamalar arasında, gerekse uygulamaların dozları arasında farklılıkların olduğu görülmüştür
Changes of the Lipid Peroxidation and Chlorophyll Amount of Green Bean Genotypes Under Drought Stress
(Academic Journals, 2010) Yasar, Fikret; Uzal, Ozlem; Ozpay, Taylan
One of the environmental stresses, and maybe one of these important drought stresses, cause deterioration of oxidative on the plant cell. Beans is one of the vegetables influenced by high level of drought. Drought stress deterioration effects on beans and its aim determines these effects either as been different or not caused by drought stress dependent on 10 item beans (Phaseolus vulgaris L.) variety. To get the hydroponic condition ready in time within 15 days, plant with 10% ratio of polyethyleen glycol (PEG 6000) were used to create drought effects. Bean leaves were harvested 6 days later after PEG implementation and were measured. The amount of lipid peroxidation production and malondyhalheit were directed towards determining the oxidative deterioration and in addition the chlorophyll amount. As a result of drought implementation, Samsun 96 (S96) and Sirik Barbunya (SB) beans genotypes have the most decreased chlorophyll amount than Gevas Sirik 57(GS57) and Oturak Barbunya (OB) genotypes. At the same time, S96 and SB genotypes have the most increased malondyhalheit (MDA) content than GS57 and OB. Also, of the total ten item of beans variety, Gevas Sirik 57 and Oturak Barbunya were least affected by drought stress, while S96 and SB genotypes showed more sensitivity to drought stress.
Characterization of Bean (Phaseolus Vulgaris L.) Cultivars Grown in Turkey by Sds-Page of Seed Proteins
(Pakistan Botanical Soc, 2011) Berber, Ismet; Yasar, Fikret
In the present research, a total 28 bean cultivars grown in Turkey were characterized by the numerical analysis of seed protein profiles obtained by using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) technique. The numerical analysis of seed protein profiles generated two major clusters at similarity levels of 82.52% or above. The first cluster included 5 reference bean cultivars at the intra-cluster similarity values of 91.25% or above. The second cluster comprised the all remaining genotypes at similarity values between 84.46% and 100%. Our results also indicated that Gina standard cultivar together with all eastern genotypes belonged to subcluster IIc, having the highest intra-cluster similarities between 95.54% and 100%. In this sense, it is suggested that probably there may be a very close genetic relationship between eastern genotypes and Gina cultivar. In conclusion, this study indicated that the numerical analysis of seed protein profiles offer no final answer to the identity of bean genotypes, however, the method can provide of extra banding pattern for the discrimination of bean genotypes cultivated in Turkey.
Determination of Anti-Oxidant Activities in Some Melon (Cucumis Melo L.) Varieties and Cultivars Under Salt Stress
(Taylor & Francis Ltd, 2006) Yasar, Fikret; Kusvuran, Sebnem; Ellialtioglu, Sebnem
The response of the antioxidant system to salt stress was studied in leaves of seedlings of four Turkish melon (Cucumis melo L.) varieties 'Besni', 'Yuva', 'Midyat' and 'Semame' and three melon cultivars 'Ananas', 'Galia C8' and 'Galia F1'. The anti-oxidant capability of plants was determined by measuring ascorbate peroxidase (APX) and glutathione reductase (GR) activities together with levels of the anti-oxidant, ascorbic acid (AA). Salt treatment increased APX activities in stress-tolerant 'Galia C8' and 'Galia F1' and in moderately tolerant 'Besni', 'Midyat' and 'Semame'. These increases were greater in salt-tolerant cultivars. On the other hand, salt treatment did not cause a significant increase in APX activity in the salt-sensitive cvs. 'Yuva' and Ananas'. GR activity increased in all varieties and cultivars grown under saline conditions compared to control plants. Increases in GR activities were higher in salt-tolerant and moderately-tolerant varieties than in salt-sensitive ones. Likewise, AA contents increased with salt treatment in all varieties and cultivars except the salt-sensitive 'Ananas'. The data indicate that melon seedlings respond to salt-induced oxidative stress by increasing both their enzymatic and non-enzymatic anti-oxidant defense systems. These increases were most clear in the salt-tolerant cultivars.
Effect of Applications of Different Potassium (K+) Doses on Antioxidant Enzyme Activities in Pepper Plants Under Salt Stress
(Polish Society Magnesium Research, 2021) Yasar, Fikret; Uzal, Ozlem
This study was carried out to determine the antioxidant enzyme activities in pepper (Capsicum annuum L. cv. Demre - long pepper) plants grown under salt stress and supplied different doses of potassium (K+). The study was conducted under controlled conditions in a 16/8 h light / dark photo period, at 25 degrees C and in a chamber with 70% humidity. After the seeds planted in pumice were germinated, seedlings with 2 true leaves were transferred into hydroponic culture containing the Hoagland nutrient solution. The K+ concentrations in the Hoagland solution were calculated as 146 mg L-1, and this level was used as a control. Other doses were set to be 20 mg L-1 lower and then 20 mg L-1 and 40 mg L-1 higher than the control dose (K1 = 126 mg L-1, K2 = 146 mg L-1, K3 = 166 mg L-1, K4 = 186 mg L-1). The salt stress level applied to the plants was induced by 100 mM sodium chloride (NaCl). Sampling for measurement and analysis was done on the 20th day of the salt application. The samples underwent the following determinations: Catalase (CAT), Ascorbate Peroxidase (APX) and Superoxide dismutase (SOD) relative to the total plant weight, and Antioxidative enzyme activities. The results indicate that K+ applications in the K3 = 166 mg L-1 and K4 = 186 mg L-1 doses reduced the impact of salt stress and the plants were less severely affected. Their antioxidant enzyme activities values in comparison to the control and other doses of K+ were quite low.
Effect of Heat Shock Treatment on Microspore Embryogenesis in Brassica Oleracea Species
(Ankara Univ, Fac Agr, 2016) Tuncer, Burcu; Cig, Arzu; Yanmaz, Ruhsar; Yasar, Fikret
Heat shock treatments are widely used to induce microspore embryogenesis in Brassica species. In this study, the effect of high temperature treatment (32 degrees C and 35 degrees C for 2 days) on microspore embryogenesis was investigated in six genotypes of Turkish white head cabbage (Yalova-1, Ercis, 177 C, 177 T, 531 C, 538 C), three genotypes of Turkish kale (Balkaya, Yanmaz, Karadere 077) and five commercial F-1 ornamental kale hybrids (Red Piegon, Victoria Piegon, Red Chidori, white Kamome, and Pink Kamome). Microspore-derived embryos formation differed depending on genotype and high temperature. The highest embryo yield was obtained as 9.92 embryo per petri dish in cv. Yalova-1, 11.13 embryo per petri dish in Pink Kamome F-1 at 32 degrees C, and 5.63 embryo per petri dish in cv. Karadere 077 at 35 degrees C.
Effect of Priming Treatment on Germination at Stressful Temperatures, Fatty Acid, Sugar Content and Enzymatic Activity of Pepper Seeds
(Ankara Univ, Fac Agr, 2010) Kaya, Gamze; Demir, Ibrahim; Tekin, Aziz; Yasar, Fikret; Demir, Koeksal
This research was conducted to investigate the effects of priming (controlled hydration at 25 degrees C for 48 h) on germination at stressful temperatures (low 15 degrees C and high 35 degrees C), sugar content, total oil, fatty acid composition and enzymatic activities of pepper (Capsicum annuum L.) seeds in two consecutive runs. The experiments were conducted on pepper cultivars of Corbaci, Sera Demre 8 and Yalova Yaglik. Results revealed that priming enhanced germination of seeds of all cultivars at stressful temperatures. The highest increase in germination was determined in cv. Corbaci at (15 degrees C) with 12% and 35 degrees C with 21%. Priming decreased total oil content dependent upon cultivars. The highest decrease in oil content by priming was determined in Demre with 2.5-3.5%. On the other hand, fatty acid composition was not changed and the highest fatty acid of the seeds was linoleic acid (78.9%) followed by palmitic (9.16-11.79%) and oleic (7.28-11.40%). Priming resulted in decrease in sucrose which declined from 0.599% to 0.390% in Yalova Yaglik. Glucose was detected as trace in both control and primed seeds. The most important effects of priming on enzymatic activities were recorded in catalase which increased remarkably with priming in both runs. Catalase activity was measured in primed seeds with 11.2 mu mol min(-1) g(-1) and in control with 9.3 mu mol min(-1) g(-1) in run 1. They were determined as 16.6 mu mol min(-1) g(-1) and 9.6 mu mol min(-1) g(-1) in run 2, respectively. Even though not the same extent, priming also increased ascorbate peroxidase and superoxide dismutase activities.
Effect of Salinity Stress on Nutrient Composition of Field Pea Genotypes (Pisum Sativum. Sp. Arvense L.)
(Medwell online, 2008) Yildinim, Buenyamin; Yasar, Fikret; Terzioglu, Oemer; Tamkoc, Ahmet; Tuerkoezue, Didem
The present study aimed to investigate the effect of different days (0, 7 and 15th days) and salt applications (salty and saltness) on nutrient element compositions of root, shoot and leaf organs of I I field pea genotypes (which are nominate pea) and 2 pea cultivars. For this aim, the data were analyzed using three-way-ANOVA (genotypes, salt application and time). In this study determining, the effects of salt stress on nutrient element compositions of these organs, although the effects of salt application, salt by variety and salt by day interactions on Cu element in root were only found to be non-significant, genotypes, salt application, days and their interactions with 2 and 3 degree for other elements were found to be significant. In root and shoot organs, salt application increased significantly Ca, Mg and Zn amounts, but other minerals decreased compared to control group. The application in leaf increased Ca and Mg, whereas others reduced.
Effect of Salinity Stress on Plant Green Weight and Nutrient Value of Soybean (Glycine Max (L.) Merrill) Cultivars
(Asian Journal of Chemistry, 2009) Tuncturk, Murat; Yasar, Fikret; Tuncturk, Rueveyde
Soil salinity is a major limitation to crop production in many areas of the world. A pot experiment was carried out with soybean cultivars to investigate the effects of salinity stress on plants growth and nutrient composition at 150 mM NaCl concentration under greenhouse conditions. Twelve soybean cultivars were exposed to salinity treatments (0 and 150 mM NaCl and control). All cultivars were harvested 45 d after plantation. Green parts of 45-day old plant weights were recorded. Soybean cultivar plants were divided into root, shoot and leaf parts for nutrient (K+, Na+, Ca2+ and Cl-) content measurements. Salinity stress negatively affected soybean cultivars and the effects varied according to the salt tolerance of the cultivars. Generally, salinity reduced the green weight. Salt stress decreased K+, Ca2+, contents and K+/Na+ rations in plants, but significantly increased Na+ and Cl- content in the roots. shoots and leaves of all cultivars. In the salt treatment, K+, Ca2+, concentration and K+/Na+ rations were high in leaf compared with those in roots and shoots. Howover, the highest increase in Na+ and Cl- contents were observed in the roots and shoot of plants. Under salinity, Mancon, Stresland and. Althow varieties retained the highest K+ and Ca2+ content in leaves, related to K+ the varieties were not effected under 150 Mm NaCl treatment.
The Effect of Salt Stress on Growth, Chlorophyll Content, Lipid Peroxidation and Antioxidative Enzymes of Pumpkin Seedling
(Academic Journals, 2011) Sevengor, Senay; Yasar, Fikret; Kusvuran, Sebnem; Ellialtioglu, Sebnem
Salinity is a major abiotic stress reducing the yield of a wide variety of crops all over the world. In order to investigate the antioxidant enzymes activity of four pumpkin genotypes (Iskenderun-4, AB-44, CU-7 and A-24) in response to salinity grown in hydroponic culture, 4 to 5 true leaf stages of pumpkin seedlings were subjected to 100 mM NaCl for 7 days. Salt stress induced changes in antioxidant enzymes, SOD, CAT, GR and APX, total chlorophyll content, lipid peroxidation and root and shoot fresh weight were measured. Salt treatment decreased root and shoots weight, chlorophyll content in salt sensitive genotypes more than salt tolerant genotypes. The four genotypes showed an increase in malondialdehyde (MDA) content under salt condition, but the increase in sensitive genotypes (CU-7 and A-24) were higher than that in salt tolerant genotypes (Iskenderun-4, AB-44). SOD, CAT, GR and APX activities increased salt stress. However these increases were higher in salt tolerant Iskenderun-4, AB-44 than salt sensitive CU-7 and A-24. These results indicate that pumpkin genotypes respond to salt induced oxidative stress by enzymatic defense systems.
Effect of Some Organic Materials on Yield and Plant Nutrient Content of Curly Leaf Salad (Lactuca Sativa Var. Crispa)
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2021) Tuga, Halide; Uzal, Ozlem; Yasar, Fikret
Caipira (Lactuca sativa var. Crispa) curly leaf salad varieties used in the study and 3 different organic materials (vermicompost, gidya, leonardit) and their control group using different doses (3%, 6%, 9%) and garden soil (0%) designed as. In this study, it is aimed to determine the effects of vermicompost, gitya and leonardite applications on plant nutrient content in different doses as organic material in salads that have an important place in human nutrition and when chemical nitrogen fertilizers are applied. According to the research findings, the effects of organic substances applied on the yield components examined were found to be significant. Although vermicompost application increased the yield approximately 2 times, no statistical difference was found between the doses. It was also determined that the effect of vermicompost on early growth in curly leaf salad was statistically significant and vermicompost gave good results especially to the intake of K+, Zn+ and Cu+ elements into the plant. In general, it has been determined that applications of gidya in terms of plant nutrient content do not give positive results.
Effects of Different Doses of Exogenous Gibberellic Acid on Total Plant Weight, Lipid Peroxidation, and Antioxidant Enzyme Activities of Eggplant Seedling Under Salt Stress
(Parlar Scientific Publications (p S P), 2019) Uzal, Ozlem; Yasar, Fikret; Yasar, Ozlem
The present study investigated the effects of different doses of gibberellic acid (GA(3)) on Aydin siyahi eggplant variety exposed to salt stress. The study was conducted to determine how applications affect plant development and metabolic activity. Eggplant seedlings were grown in a temperature-controlled hydroponic system in Hoagland nutrient solution. When they were 3 weeks old, they were exposed to salt stress (100 mM NaCl). Subsequently, they were treated with different doses of GA(3) (2.5, 5.0, 7.5, and 10 ppm). Leaves were harvested on the 15th day of the experiment. Fifteen days after the induction of salt stress, physiological (total plant weight) and biochemical (antioxidant enzyme activity, malondialdehyde [MDA] content, and chlorophyll content) changes were measured to determine the effect of the GA(3) treatments on plant growth and metabolic activity. In the NaCl plus high GA(3) treatment, the plant chlorosis level and degree of salt-induced damage decreased. In the NaCl plus high GA(3) (10 ppm treatment), plant development slowed due to salt-induced stress, but symptoms of stress, such as curling or chlorosis, were not detected in plant leaves. All doses the GA(3) treatments reduced antioxidant enzyme activities and MDA contents. In summary, the application of GA(3) to eggplant partially suppressed the adverse effects of salt stress on plant development and metabolic activities, and this effect was more apparent at a GA(3) dose of 10 ppm. Therefore, GA(3) may provide direct and indirect protection against salt stress.
Effects of External Melatonin Treatments on Morphological and Physiological Changes in Cucumber (Cucumis Sativus L.) Seedlings Against Chilling Stress
(Springer, 2023) Uzal, Ozlem; Baslak, Lutfullah; Yasar, Fikret
This study aimed to determine chill resistance and to explain how chilling stress affects metabolic processes by applying different doses of melatonin to Beith F1 hybrid cucumber (Cucumis sativus L.). In addition, experiments were conducted to understand the response of plants with and without melatonin to chilling stress and their adaptation mechanisms against chilling. Melatonin-containing (0, 1, 10, 20, 30 and 40 mu M) distilled water was sprayed to the leaves of chilling-treated seedlings with 3-4 true leaves. One full day after melatonin treatments, half of the plants were exposed to chilling stress in a climate chamber for 15 days and the other half was kept in the climate chamber under normal conditions (25/20 degrees C, 12/12 light/dark photoperiod). Plants exposed to chilling stress were kept in an incubator for 15 days at 5 +/- 1 degrees C dark (12 h)/10 +/- 1 degrees C light (12 h), then samples were taken. Some plant growth parameters were measured, and some biochemical analyses were performed. In terms of plant total fresh weights, it was observed that chilling stress and plants treated with 30-40 mu M melatonin showed limited plant growth and development and thus were less influenced by chilling stress. Melatonin treatments slowed down the growth rate of plants under cold stress, but, as it increased antioxidant enzyme activities, it protected plants from the destructive effect of stress and reduced the formation of malondialdehyde, a product of lipid peroxidation. In addition, there was no decrease in the amount of chlorophyll of the plants since malondialdehyde prevents the degradation of chlorophyll pigments. It was concluded based on present findings that external melatonin treatments could be used as a physiologically effective auxiliary application to reduce harmful effects of chilling stress.
Effects of Jasmonic Acid and Salt Applications on Antioxydative Enzyme Activities of the Eggplant Seedlings Grown in Vitro Culture
(Elsevier Sci Ltd, 2011) Gunalp, Banu; Horasan, Ozge; Yasar, Fikret; Kusvuran, Sebnem; Tipirdamaz, Rukiye; Ellialtioglu, Sebnem
Effects of Salicylic Acid Application on Wrky Gene in Pepper (Capsicum Annuum L.) Seedling
(Parlar Scientific Publications (p S P), 2020) Ahmed, Mohammed Ahmed; Karipcin, Muhemet Zeki; Yasar, Fikret
This project was developed to investigate the contribution of salicylic acid to development of pepper seedlings grown in low temperature (0 degrees C) conditions. The research was carried out in the controlled plant growing cabinet in the research-investigation area of the Department of Horticulture, Faculty of Agriculture, Siirt University. As a vegetable material, Urartu F1 pepper type (capia) which is used in greenhouse cultivation has been used. As a dose of different salicylic acid; 0.01 and 0.05 mmol doses were applied. The dose of 0 mmol salicylic acid was used as control group. Application frequency; It was applied 1 time, 2 times and 3 times. 3 different cold application times were also investigated; 24 hours, 48 hours and 72 hours. The experiment was designed in randomized plots and 3 replications. In the pepper seedlings the effect of the seedlings on the WRKY genes were investigated. Considering the administration of both salicylic acids, it was found that 0.05 mmol dose had a clearer and more understanding response to the expression of WRKY gene.
Effects of Salt Stress on Ion and Lipidperoxidation Content in Green Beans Genotypes
(Asian Journal of Chemistry, 2007) Yasar, Fikret
The response of 13 green bean genotypes used in this study to salt stress was found differently. Taking into consideration of all genotypes, generally, the genotypes showing better growth under salt stress had lower Na, higher K and Ca contents. Of green bean genotypes used this study; Ca contents of genotypes having higher fresh weight was found higher than those of genotypes having lower fresh weight under salt stress. Tolerant genotypes had higher K+/Na+ and Ca2+/Na+ ratios than sensitive genotypes. Differences between MDA contents of 13 green bean genotypes under grown salt stress were found significant. The lowest MDA content was obtained in GS57 showing the best growth under salinity. At the same time, this genotype had the highest K+ and Ca2+ content and the second lowest Na+ content. ES18 and GB64 genotypes also presented the same behaviours in salinity.
Effects of Two Different Light Intensities on Plant Growth, Ion Uptake and Distribution in Tomato Plants
(Polish Society Magnesium Research, 2022) Yasar, Fikret; Akin, Seyfettin; Uzal, Ozlem
This study was carried out to determine relationships between light intensity and nutrient elements by examining the growth parameters, ion uptake and distribution between plant organs of tomato plants under controlled conditions. In the study, seedlings of an indeterminate Adamset F1 hybrid tomato variety were used. The seedlings were grown by applying two different light intensities: 400 mu mol m(-1) s(-1), which is the optimum light intensity for tomatoes, and 800 mu mol m(-1) s(-1), which is twice the optimum light intensity. In this study, LED lamps, which emit light close to sunlight, were used as the light source. Sampling for measurement and analysis was done on the 40(th) day of the application. The Hoagland nutrient solution was replenished every week. Leaf weight, leaf number, stem weight, stem length, internode distance, stem thickness and root weight of the plants taken on the 40th day were measured. Concentrations of macro- and micronutrients (N, P, K, Ca, Mg, Fe, Mn, Zn, Cu) in the root, stem and leaf samples of plants were also measured. The root, stem and leaf weights of the plants that were treated with two different light intensities were found to be different. The highest root, stem and leaf weights were observed in plants with high light exposure. It has been found that light intensity is effective in stimulating the ion uptake and distribution, and especially the amount of microelements in all three organs of tomato plants analyzed, and there was higher uptake under high light application compared to optimum light application.
Enzyme Activities of Certain Pumpkin (Cucurbita Spp) Species Under Drought Stress
(Parlar Scientific Publications (p S P), 2014) Yasar, Fikret; Uzal, Ozlem; Kose, Serif; Yasar, Ozlem; Ellialtioglu, Sebnem
The aim of this study is to investigate the relationship between the drought tolerance capacity and antioxidant enzyme activity of 38 genotypes of different pumpkin species from different regions of Turkey. The seedlings from 38 different genotypes were put into pots containing Hoagland's solution to be stored in cultivation rooms under controlled climatic conditions. A 15% polyethylene glycol 6000 (PEG 6000) solution was added to the Hoagland's solution to create an osmotic potential equivalent to -0.60 M=Pa for the drought test application. The leaf weights were determined following the test under drought and control conditions. The relative water content, chlorophyll content, and antioxidative enzyme activities (superoxide dismutase, catalase, and ascorbate perwddase) were investigated. As a result, it was observed that the enzyme activities are extremely vital in the drought tolerance of the pumpkin genotypes; such as under dry conditions, the drought tolerant pumpkin genotypes use antioxidative enzymes more actively compared to the drought susceptible genotypes.