Browsing by Author "Yasar, Semih"

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Assessment of the Wheatgrass Effects on Antioxidant Capacity and Dna Damage in Rats With Experimental Renal Failure
(Parlar Scientific Publications (p S P), 2019) Mis, Leyla; Comba, Bahat; Basbugan, Yildiray; Yasar, Semih; Comba, Arzu
In this study, it was aimed to evaluate the effect of wheatgrass on antioxidant capacity and DNA damage in rats with renal failure. In the study, 200-300 g 24 healthy Wistar-Albino male rats obtained from YYU Experimental Animal Unit were used. The rats were randomly divided into 4 groups and each group was formed with 6 rats. Groups were allocated as; 1. Control Group, 2. Renal Failure Group (Genta Group) 3. Renal Failure + Wheatgrass Group (Genta +Wheatgrass) 4. Wheatgrass Group. Gentamicin sulfate was given intraperitoneal at a dose of 50 mg/kg for 1 week and once a day to the groups with renal failure (Groups 2 and 3). Wheat grass extract (10 ml/kg) was added to the drinking water of the wheatgrass group rats (3rd and 4th groups) once a day. This practice lasted for 4 weeks. Total oxidant, antioxidant quantities, DNA damage were evaluated at the end of 4 weeks. The result that wheatgrass could be useful in renal failure was reached.
Comparison of Effects of High and Low Dose Paracetamol Treatment and Toxicity on Brain and Liver in Rats
(Kare Publ, 2020) Oksuz, Ersoy; Yasar, Semih; Erten, Remzi; Arihan, Okan; Oto, Gokhan
OBJECTIVE: Paracetamol is thought that it acts by inhibiting the central cyclooxygenase (COX) enzyme; its mechanism of action is still not fully explained. Although its most important side effect is hepatoxicity, it is thought to cause toxicity on the brain in recent years. The present study aims to investigate the treatment and toxic effects of low and high doses of paracetamol on the liver and brain. METHODS: Wistar-albino rats were used in this study. At doses of 20-500 mg/kg, paracetamol was administered intraperitoneally once a day for one and three days. The brain and liver were used for immunohistochemical evaluation using COX-3, prostaglandin E-2 (PGE(2)) and caspase 3 antibodies and for total antioxidant (TAS), total oxidant ( TOS) and oxidative stress index (OSI) measurements. Results were evaluated using the Kruskal Wallis test (SPSS ver.24). RESULTS: The liver COX-3 levels were significantly lower in both groups with higher doses (p<0.05). In the brain, there was no statistically significant difference in COX-3 levels between the groups. There was no statistically significant difference in PGE(2) levels in the liver and brain between the groups (p>0.05). The caspase 3 level in the liver was statistically significantly higher in the low dose group compared to the other groups (p<0.05). In both liver and brain, OSI values were significantly higher in the 3-day high-dose group compared to others (p<0.05). There was no statistically significant difference between the groups in ALT and AST values (p>0.05). CONCLUSION: The results of our study show that paracetamol inhibits the COX-3 enzyme in the liver but has no effect in the brain, and COX-3 does not have an effect on PGE(2). Paracetamol causes apoptosis in the liver only in low doses; higher doses may cause toxicity by increasing oxidative stress, especially in the brain.
The Effect of Coq10 and Vitamin E on Serum Total Sialic Acid, Lipid-Bound Sialic Acid, Some Trace Elements and Minerals in Rats Induced With Doxorubicin
(Academic Journals, 2011) Oto, Gokhan; Ekin, Suat; Yasar, Semih; Ozdemir, Hulya; Yur, Fatmagul
This study was designed to evaluate the effect of CoQ(10) and vitamin E on serum total sialic acid (TSA), lipid bound sialic acid (LSA) and some elements in rat administered doxorubicin (DXR). Cu levels were increased in the group treated with DXR + vitamin E in comparison with DXR (p<0.05) and CoQ(10) groups (p = 0.001). Furthermore, copper levels were increased in the group treated with DXR + CoQ10 in comparison with CoQ(10) group (p < 0.05). Zn levels were decreased in the group treated with DXR + vitamin E in comparison with CoQ(10) group (p < 0.05). Mg levels were decreased in subjects treated with DXR + vitamin E in comparison with the control group values (p < 0.05). Particularly, the observed increase in Cu levels in rats from DXR + vitamin E group might be due to the decrease of vitamin E. However, the oxidative damage could be as a result of DXR occurence and may be helpful to clinicians in chemotherapy using anthracycline.
The Effects of Nettle Herb (Urtica Dioica L.) on the Levels of Trace Elements in the Rabbit Fur Treaties With 7,12-Dimetylbenzanthracene
(Kafkas Univ, veteriner Fakultesi dergisi, 2009) Tuerel, Idris; Ertekin, Ali; Oto, Goekhan; Celikezen, F. Caglar; Yasar, Semih
In this study the effect of nettle herb (Urtica dioica L.) on the levels of trace element (Zn, Cu, Mn, Fe) in the rabbit fur treated with 7,12-dimetylbenzanthracene (DMBA) were investigated. The rabbit were divided into three experimental groups and one control group, each group contained seven rabbits. DMBA (0.5 ml/kg/day) were administreated IM to group A as carcinogenic compound. DMBA (0.5 ml/kg/day) were administreated IM as carcinogenic compound plus methanol extract of nettle herb (0.2 ml/kg/day) to group B, DMBA (0.5 ml/kg/day) were administreated IM as carcinogenic compound plus water extract of nettle herb (0.5 ml/kg/day) to group C and 10% dimethylsulphoxide (0.5 ml/kg/day) dissolved in normal saline solution were administrated to control group. All of the treatments were performed for five mounths to each group. The decrases in Cu levels were significant when compared with that of control group (P < 0.01). The decrases in Fe levels in water extract of DMBA+Urtica dioica L. was statistically significant compared with that of control group (P < 0.05). In conclusion. fur trace element levels of groups treated with methanol or water extract of Urtica dioica L. were less compared with that of control group but were higher compared with that of DMBA group. It was thought that Urtica dioica L. can have protective effect against damage caused by DMBA.
Element Status in Different Breeds of Dogs
(Humana Press inc, 2008) Mert, Handan; Mert, Nihat; Dogan, Inci; Cellat, Mustafa; Yasar, Semih
The serum levels of copper, zinc, iron, manganese, nickel, cadmium, cobalt, sodium, potassium, calcium, and magnesium were determined in seven different breeds of dogs: Pointer, Poodle, Setter, Labrador Retriever, Golden Retriever, German Shepherd, and Mallinois. Only slight variations were found among the different breeds, and the results presented in this study can be used for laboratory studies in veterinary science.
Evaluation of the Effect of Pomegranate Flowers on Antioxidant Activity and Bdnf Levels in Experimental Renal Failure in Rats
(Parlar Scientific Publications (p S P), 2021) Mis, Leyla; Baydas, Burhanettin; Yasar, Semih
This study was performed with the aim of researching the effects of pomegranate flower on antioxidant activity and BDNF levels in rats with experimental renal failure induced. With this aim, 42 adult Wistar albino rats were used. Rats were divided into 6 groups containing 7 rats according to the amount of pomegranate flower administered. The groups were named control, 20% pomegranate flower, 10% pomegranate flower, 20% pomegranate flower+gentamicin, 100% pomegranate flower+gentamicin and gentamicin group. Diets were prepared containing 20% and 10% pomegranate flower. The relevant groups were fed with this diet for 4 weeks. In the final week, gentamicin was administered by ip injection for 8 days. The lipid peroxidation (malondialdehyde, MDA), glutathione (GSH), Glutathhion peroxidase (GSH-Px) and superoxide dismutase (SOD) levels in blood were researched. Compared with the control group, rats administered different doses of pomegranate flower had significant reductions observed in serum lipid peroxidation levels, while there was clear increase in GSH,GSH-Px and SOD levels identified. Groups administered 20% pomegranate flower had an increase identified in BDNF levels. In conclusion, our findings support the view that pomegranate flower may have strong antioxidant activity and increase serum BDNF levels.
Investigation of the Pharmacological, Behavioral, and Biochemical Effects of Boron in Parkinson-Indicated Rats
(C M B Assoc, 2022) Ozdemir, Hulya Sagmanligil; Yunusoglu, Oruc; Sagmanligil, Vedat; Yasar, Semih; Colcimen, Nese; Goceroglu, Rezzan Temelli; Catalkaya, Ege
Parkinson's disease (PD) is a progressive neurodegenerative disorder of the central nervous system. In different studies, it has been investigated that boric acid has positive effects on different mechanisms that are important in PD. The aim of our study was to investigate the pharmacological, behavioral and biochemical effects of boric acid on rats with experimental PD with Rotenone. For this purpose, Wistar-albino rats were divided into 6 groups. Only normal saline was applied subcutaneously (s.c) to the first control and sunflower oil to the second control group. Rotenone was administered (s.c) to 4 groups (groups 3-6) at a dose of 2 mg/kg for 21 days. Only rotenone (2mg/kg, s.c) was administered to the third group. Boric acid was administered intraperitoneally (i.p.) at 5 mg/kg, 10 mg/kg, and 20 mg/kg to groups 4, 5, and 6, respectively. During the study, behavioral tests were applied to the rats, and then histopathological and biochemical analyzes were performed from the sacrificed tissues. According to the data obtained, a statistically significant difference (p<0.05) was observed between the Parkinson's group and the other groups in motor behavior tests, excluding the catalepsy test. Boric acid exhibited dose-dependent antioxidant activity. As a result of the histopathological and immunohistochemical (IHC) examination, a decrease in neuronal degeneration was observed at the increasing doses of boric acid, while gliosis and focal encephalomalacia were rarely encountered. There was a significant increase in tyrosine hydroxylase (TH) immunoreactivity, especially in group 6, with a dose of 20 mg/kg of boric acid. From these results, we conclude that the dose-dependent effect of boric acid may protect the dopaminergic system with antioxidant activity in the pathogenesis of PD. However, the effectiveness of boric acid on PD needs further investigation in a larger, more detailed study using different methods.
Plantago Major Protective Effects on Antioxidant Status After Administration of 7,12-Dimethylbenz(a)anthracene in Rats
(Asian Pacific Organization Cancer Prevention, 2011) Oto, Gokhan; Ekin, Suat; Ozdemir, Hulya; Demir, Halit; Yasar, Semih; Levent, Abdulkadir; Kaki, Baris
Aim: The present study was designed to evaluate effects of Plantago major extract on oxidative status in Wistar albino rats administrated 7,12-dimethylbenz(a)anthracene (DMBA). Methods: Rats were divided into three equal groups of 6 animals each: Group 1 controls, group 2 treated with DMBA (100 mg/kg, single dose) and group 3 receiving the DMBA and the aqueous extract at 100 mg/kg/d for 60 days. Results: Significant decrease in catalase (P<0.05), carbonic anhydrase (p <= 0.01), reduced glutathione (GSH) (P<0.01) and total protein (P<0.01) values was observed in the DMBA group compared with the healthy controls and DMBA + Plantago major groups. Conclusion: The results suggest preventive effects of Plantago major on DMBA induced oxidative damage in Wistar albino rats that might be due to decreased free radical generation.
Role of Sedative-Hypnotic Agents in Neurodegeneration: Effects of Midazolam and Thiopental on Apoptosis and Oxidative Stress Expression in Neonatal and Adult Rat Brains
(Turkish Neurosurgical Soc, 2022) Soyalp, Celaleddin; Oksuz, Ersoy; Gorgisen, Gokhan; Gulacar, Ismail Musab; Yasar, Semih; Tuncdemir, Yunus Emre; Acun Delen, Leman
AIM: To investigate the effects of midazolam (MDZ) and thiopental on neonatal and adult rat brains. MATERIAL and METHODS: The study included adult and 7-day-old rats that were administered 9 mg/kg of MDZ, 60 mg/kg of thiopental, or both. The Bax, procaspase-3, and caspase-3 levels were assessed using Western Blot analysis and the total oxidative stress index (OSI) values were measured spectrophotometrically. RESULTS: The procaspase-3 and caspase-3 levels were 12% and 6% lower in the neonatal MDZ group when compared to the control group. The Bax, procaspase-3, and caspase-3 levels were higher in the neonatal thiopental group by 25%, 4%, and 34%, and in the MDZ group by 16%, 19%, and 43% when compared to the neonatal control group. In the adult rats, the caspase-3 levels were 10 times higher in the MDZ group when compared to the control and thiopental groups. Moreover, the caspase-3 levels were 7 times higher in the adult thiopental group when compared to the control group. The OSI values in the neonatal rats were significantly higher in the neonatal MDZ and neonatal thiopental groups when compared to the control group (p<0.05). Similarly, the OSI values in the adult rats were significantly higher in the neonatal MDZ and neonatal thiopental groups when compared to the control group CONCLUSION: MDZ and thiopental may promote apoptosis and oxidative stress, and thereby result in neurotoxicity, with MDZ showing a greater effect in adults and thiopental showing a greater effect in neonates.
Zigzag and Armchair Aln Nanotubes as Anode Materials for Mg-Ion Batteries: Computational Study
(Elsevier, 2020) Yasar, Semih; Sogutlu, Inci; Mert, Handan; Mert, Nihat; Vessally, Esmail; Lin, Yuan
We studied the potential application of a zigzag and an armchair AlN nanotube (AlNNT) as an anode material for the Mg-ion batteries (MIBs) using B3LYP-gCP-D3/6-31G* model chemistry. The Mg2+ adsorption energy on the zigzag or armchair AlNNTs is -217.2 or -234.9 kcal/mol, but the Mg atom interaction is much weaker and the adsorption energy is -10.5 or -5.3 kcal/mol. The dispersion term is much more important for the Mg interaction compared to the Mg2+. This term includes 68.6% of the Mg atom adsorption energy on the zigzag tube while its contribution for Mg cation adsorption is about 3.8%. The maximum energy barrier for an Mg2+ migration and cell voltage are 6.1 kcal/mol and 4.47 V for zigzag and 5.3 kcal/mol and 4.95 V for armchair AlNNT, respectively. Thus, the AlNNTs provide a great ion mobility leading to a faster charge/discharge rate because of small energy barriers. The larger cell voltage, and higher ion mobility suggest the armchair AlNNTs as a plausible anode material for application in the anode of MIBs, compared to the zigzag AlNNT. The effect of Mg/Mg2+ adsorption on the electronic properties of AlNNTs is also discussed.