Browsing by Author "Yeltekin, A.Ç."

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Effect of Fungicide Toxicity on Apoptosis, Dna Damage, and Antioxidant Enzymes in Van Fish
(University of Punjab (new Campus), 2023) Yeltekin, A.Ç.
The extensive use of tebuconazole, an azole group fungicide, causes undesirable toxicity in non-targeted organisms, including fish in aquatic environments. We investigated metabolic responses by observing apoptosis (caspase- 3), DNA damage (8-hydroxy-2-deoxyguanosine (8-OHdG)), malondialdehyde (MDA), and antioxidants (SOD, CAT, GSH-Px) activity in Van fish kidney and muscle tissue after 24., 48., 72., and 96. h of tebuconazole exposure at a concentration of 2.5 mg/L. The obtained results indicated that caspase-3, 8-OHdG, and MDA levels significantly increased compared to the control (p < 0.05). The kidney and muscle tissues indicated a significant decrease in antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)) compared to the control (p< 0.05). Therefore, these data may reflect one of the molecular pathways that play a role in tebuconazole toxicity. Copyright 2023 by the authors. Licensee Zoological Society of Pakistan.
Investigation of Bdnf, Apoptosis and Cholinergic System Enzymes in Glioblastoma Multiforme Patient Serum
(Medical Project Poland, 2024) El-Tekreti, S.A.A.; Yeltekin, A.Ç.; Aycan, A.; Tas, A.
Background: Glioblastoma Multiforme (GBM) is the most common type of brain tumor and it is an aggressive form of adult brain cancer that is globally fatal. Unfortunately, the poor prognosis of patients with glioblastoma and current treatments do not appear to be very effective against glioblastoma as the disease is still fatal. Our study aimed to explore new ways of diagnosing the disease to develop a treatment for this deadly disease. Method: This study included 40 individuals (20 glioblastoma multiforme 20 control groups). The blood samples were taken from the study groups, and biochemical analysis was performed. The groups were then compared for statistical significance. Results: Our study aimed to determine the relationship between enzymes, some parameters levels and glioblastoma multiforme. When compared to the control group, the levels of GSH-Px, SOD, CAT, AChE, and BChE were decreased in patients with GBM, and the levels of MDA caspase-3, BDNF, TNF alfa, and 8-OHdG were increased. Conclusion: The results noted here point out that Apoptosis, Cholinergic System Enzymes, and BDNF are good and accurate markers in Glioblastoma Multiforme to know of diagnosing the disease. Will make it possible to use characteristics seen in glioblastoma multiforme as indicators for assessing tumor aggressiveness, as well as to get a better understanding of Proteins that are involved in apoptosis as well as anti-apoptotic proteins, allowing for the creation of novel molecularly based treatments. © Oncology and Radiotherapy.
Therapeutic Effect of N- Acetyl Cysteine as an Antioxidant on Rainbow Trout's Brain in Cypermethrin Toxicity
(Elsevier Ltd, 2019) Alak, G.; Yeltekin, A.Ç.; Özgeriş, F.B.; Parlak, V.; Uçar, A.; Sait Keleş, M.; Atamanalp, M.
The aim of this study was to investigate the therapeutic effect of N-acetylcystein (NAC) against oxidative stress induced by Cypermethrin pesticide in rainbow trout (Oncorhynchus mykiss). The experiment was designed as 5 groups (A, B, C, D, and E). Group A was organized as control group and had no treatment. The other groups were treated with Cypermethrin for 14 days. At the end of this period, Groups B (1.0 mM NAC) and D (0.5 mM NAC) was performed with NAC for 96 h. Group C was not administered NAC, the recovery process was evaluated with this group. Group E was exposed to cypermethrin during 14 days and sampled. Acetylcholinesterase (AChE), malondialdehyde (MDA), glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), paraoxonase (PON), arylesterase (AR), myeloperoxidase (MPO) activities, oxidative DNA damage (8-hydroxy-2′-deoxyguanosine (8-OHdG)), caspase-3 levels, and trace elements contents analyses were performed in all fish brains. According to the results, MDA, MPO, 8-OHdG and caspase-3 levels were significantly decreased compared to the other groups (pesticide and recovery) (p < 0.05), AChE, SOD, CAT, GPx, PON, and AR activities increased (p < 0.05). In brain tissue, no statistically significant difference was observed in trace element analysis of all application groups. According to the obtained data, the positive effect of N-acetylcysteine on protein synthesis, detoxification, and diverse metabolic functions against cypermethrin toxicity has been more effective in 1.0 mM NAC. NAC has important therapeutic effect on pesticide-induced neurotoxicity for fish in terms of all data. It was concluded that NAC has an antioxidant effect against pesticide-induced oxidative stress and the selected biochemical markers are useful for such studies. © 2019 Elsevier Ltd
Toxic Effects of Sodium Lauryl Sulfate on Antioxidant Defense System and Dna Damage in Fish Primary Hepatocyte Cultures
(Sciendo, 2022) Yeltekin, A.Ç.; Oǧuz, A.R.
Synthetic detergents which have a major role in environmental pollution accumulate over time and reach levels that harm nature. The surfactants which are abundantly used as cleaning components are discharged into the Van Lake with the sewage water. These chemicals accumulating in the lake may reach a level that could affect the only fish species of the lake, the Van fish. This study aimed to determine the antioxidant levels of Van fish hepatocyte cell culture medium treated with sodium lauryl sulphate (SLS) and to assess the DNA damage. The effect of SLS was assessed by its dose (1×10-5, 1×10-6, 1×10-7 M) and treatment time (24 h, 48 h). Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), and DNA damage (8-OHdG) were determined in the SLS hepatocyte culture. SOD and GSH-Px were higher on 24 h and 48 h compared to the control group. A significant increase was observed in CAT level in the first 24 h, especially in 1×10-6 and 1×10-5 M concentration. At 48 h, it was observed that the CAT level decreased significantly as the concentration increased. It was determined that MDA and 8-OHdG levels increased depending on concentration and time. In conclusion, different concentrations of SLS affected antioxidant levels in the primary hepatocyte culture of Van Fish and were found to cause an increase in the levels of MDA and 8-OHdG. © 2022 Asll Ç. Yeltekin et al., published by Sciendo.