Browsing by Author "Yener, Zabit"

Now showing 1 - 20 of 47

Antitumor Activity of Urtica Dioica Seed Extract on Diethylnitrosamine-Induced Liver Carcinogenesis in Rats
(Natl inst Science Communication-niscair, 2024) Keles, Omer Faruk; Huyut, Zubeyir; Arslan, Mevlut; Yildizhan, Kenan; Yener, Zabit
Hepatocellular carcinoma (HCC) is a significant health problem for human life; therefore, new therapeutic approaches are essential. In vitro studies have shown that the extract of Urtica dioica seed extract (UDSE) may be a crucial protective agent to prevent HCC. Therefore, this study aimed to investigate the antitumor efficacy of UDSE in the process of carcinogenesis induced by diethylnitrosamine (DENA). The antitumor efficacy was evaluated by examining liver tissue histopathology and expression of Hep par-1, alpha-fetoprotein (AFP), caspase-3, and inducible nitric oxide synthase (iNOS) in the liver tissue and activities/levels of aspartate transaminase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH), carbohydrate antigen (CA) 15-3, CA 19-9, CA 125-II in the serum, and also total oxidative stress (TOS), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), total antioxidant status (TAS) in the serum and liver. In addition, real-time PCR was used to evaluate the levels of tumor necrosis factor-alpha (TNF-alpha), interleukin (IL-1 beta, IL-6), and proliferating cell nuclear antigen (PCNA) in liver tissue. It was observed that DENA application increased liver function tests, cancer markers, apoptosis, and proinflammatory cytokine levels, but UDSE application and DENA suppressed these increases. The findings and histopathological data demonstrated that the UDSE has a very significant antitumor efficacy on the process of DENA-induced hepatocellular carcinogenesis, which appears to be attributable to its antioxidant, anti-apoptotic, and anti-proliferative activity.
Biomedical Effects of Laurus Nobilis L. Leaf Extract on Vital Organs in Streptozotocin-Induced Diabetic Rats: Experimental Research
(Elsevier Sci Ltd, 2021) Mohammed, Rebin Rafaat; Omer, Abdullah Khalid; Yener, Zabit; Uyar, Ahmet; Ahmed, Avin Kawa
Diabetes mellitus (DM) has been treated with herbs for centuries and many herbs reported to exert antidiabetic activity. Laurus nobilis is an aromatic herb belonging to the Lauraceae family, commonly known as bay. This study aimed to investigate the activity of Laurus nobilis leave extracts on histopathological and biochemical changes in beta-cells of streptozotocin (STZ)-induced diabetic rats. Thirty healthy adult male albino rats were included in the study and divided equally into 5 groups for 4 weeks as follow; control group (C), diabetic group (D), diabetic Laurus nobilis extract group (DLN), Laurus nobilis extract group (LN) and diabetic acarbose (DA) group. Histopathologically, D group rats exhibited various degenerative and necrotic changes in their liver, pancreas and kidney, whereas the DLN rats had nearly normal histology. Insulin immunostaining in the pancreatic beta cells was decreased in the D group compared to the C group, whereas the DLN group was similar to the C group. The glucose concentration decreased significantly in both diabetic rats treated with L. nobilis and acarbose (p < 0.05). Additionally, the levels of aspartate aminotransferase (AST), gamma-glutamyltransferase (GGT) and alanine aminotransferase (ALT) enzyme were significantly decreased in both diabetic rats treated with L. nobilis and acarbose, compared to the D group (p (>) 0.05). Outcomes of this study said that leave extracts of L. nobilis has valuable effect on blood glucose level and ameliorative effect on regeneration of pancreatic islets, it also restored the altered liver enzymes, urea, creatine kinase, total protein levels, calcium and ferritin to near normal.
Chemopreventive Efficacy of Juniper Berry Oil (Juniperus Communis L.) on Azoxymethane-Induced Colon Carcinogenesis in Rat
(Routledge Journals, Taylor & Francis Ltd, 2021) Yaman, Turan; Uyar, Ahmet; Komuroglu, Ahmet Ufuk; Keles, Omer Faruk; Yener, Zabit
The aim of this study was to investigate the chemopreventive effects of juniper berry (JB) oil on azoxymethane (AOM)-induced colon cancer in rats. Thirty-two male Wistar albino rats were allocated into four groups: Control, AOM, AOM?+?JB, and JB groups. Whereas the control group was fed with standard pellet feed, the AOM and AOM?+?JB groups were administered of AOM (15?mg/kg body weight) subcutaneously once every 2 weeks for 10?weeks. AOM?+?JB and JB groups additionally received JB oil (100??l/kg) orally. At the end of the 16-week experimental period, blood and tissue samples were obtained from the rats following necropsy. The macroscopic findings showed that the application of JB oil significantly decreased adenoma and adenocarcinoma formation both numerically and dimensionally. Immunohistochemically, CEA, COX-2, and Ki-67 immune-expressions decreased, and the immune-expression of caspase-3 increased in AOM?+?JB treated rats. Additionally, JB oil supplementation ameliorated antioxidant defense systems and lipid peroxidation within the colon tissue of AOM?+?JB treated rats. These results reveal that the JB oil acted as a chemopreventive dietary agent, inhibiting cell proliferation and COX-2 expression and inducing apoptosis, resulting in a significant reduction in colon tumor formation.
Comparison of the Protective Effects of Curcumin and Caffeic Acid Phenethyl Ester Against Doxorubicin-Induced Testicular Toxicity
(Wiley, 2021) Huyut, Zubeyir; Alp, Hamit Hakan; Yaman, Turan; Keles, Omer Faruk; Yener, Zabit; Turkan, Fikret; Ayengin, Kemal
Whether testicular toxicity is mediated by matrix metalloproteinases (MMPs) is an important question that has not been examined. This study investigated the suppressive effect of curcumin and caffeic acid phenethyl ester (CAPE) on oxidative stress, apoptosis, and whether MMPs mediate doxorubicin (DOX)-induced testicular injury. Male rats were randomly divided into eight groups (n = 8 per group). The groups were as follows: sham, dimethyl sulphoxide (100 mu L), DOX (3 mg/kg), CAPE (2.68 mg/kg), curcumin (30 mg/kg), DOX+CAPE (3 mg/kg DOX and 2.68 mg/kg CAPE), DOX+curcumin (3 mg/kg DOX and 30 mg/kg curcumin) and DOX+CAPE+curcumin (3 mg/kg DOX, 2.68 mg/kg CAPE and 30 mg/kg curcumin). Injections were administered daily for 21 days. The oxidative stress, MMPs, proinflammatory cytokines and apoptotic markers in the DOX group were higher than the sham group (p < .05); these measures were lower in the groups treated with CAPE and curcumin together with DOX compared with the DOX group (p < .05). The results showed that MMPs mediated DOX-induced testicular injury, but CAPE and especially curcumin suppressed testis injury and cell apoptosis by suppressing DOX-induced increases in MMPs, oxidative stress and proinflammatory cytokines. However, curcumin exhibited more pronounced effects than CAPE in terms of all studied parameters.
Cytoprotective Effects of Boric Acid and Coenzyme Q10 Therapy on Induced Pulmonary Fibrosis in Response To Intratracheal Administration of Bleomycin in Rats
(Parlar Scientific Publications (p S P), 2013) Oto, Gokhan; Ekin, Suat; Celikezen, Fatih Caglar; Yener, Zabit; Tanritanir, Pinar; Ozdeinir, Hulya; Bayramoglu, Mahire
This study was designed to examine cytoprotective effects of boric acid and CoQ(10) on a model of bleomycin-induced lung fibrosis. A total of 32 female Wistar albino rats (200-250 g, n=8) were randomly divided into four groups (control, bleomycin, bleomycin + boric acid, and bleomycin + boric acid + CoQ(10)). Rats in the control group were given equal volumes of saline intratracheally. Lung fibrosis was induced by intratracheal administration of bleomycin hydrochloride (7.5 mg/kg in 0.9% NaCl) to rats under anaesthesia. In the treatment groups, the rats were treated with boric acid (10 mg/kg per day; perorally) and CoQ(10) (4 mg/kg per day; intraperitoneally) for 30 days. All animals were sacrificed at the end of the experiment. The lung, liver, kidney, and spleen tissues were excised, taken for histopathological evaluation, and stored for the measurement of SOD and GSH-Px activities with trace element and minerals. As a result, boric acid and boric acid + CoQ(10) had preventive roles on bleomycin-induced lung fibrosis in rats.
Deneysel Olarak Ccl4 Verilen Ratlarda Vitamin E + Selenyum ve Nigella Sativa (Çörekotu)' Nun Karaciğer Nekrozunu Engelleyici Etkisi
(1990) Şahin, Ali; Alkan, Musa; Dede, Semiha; Oto, Gökhan; Dağoğlu, Gürdal; Yener, Zabit
Bu çalışmada, ratlarda karbontetraklorür ($CCL_4$) ile deneysel oluşturulan karaciğer nekrozunun engellenmesinde Nigella sativa (çörekotu)'nın etkisi araştırıldı. Çörekotunun antioksidan etkisinin karşılaştırılabilmesi amacıyla, yaygın olarak bilinen antioksidan vitamin E ve selenyum minerali seçildi. Denemede 40 adet rat kullanıldı ve her grupta sekiz rat bulunacak şekilde A,B,C,D,E gruplarına ayrıldı. A grubu kontrol olarak belirlendi ve deneme boyunca diğer gruplara içinde $CCL_4$ verilen aynı miktar parafin likid intraperitoneal (i.p.) uygulandı. B grubuna yalnızca likid parafin içinde $CCL_4$ i.p. verildi. C grubuna, i.p. olarak likid parafin içinde $CCL_4$ ve vitamin E+selenyum kombinasyonu ticari bir preparat i.m. uygulandı. D grubuna i.p. olarak likid parafin içinde $CCL_4$, i.m. olarak vit E+Se preparatı ve standart rat peletine %10 oranında katılmış çörekotu ad libitum verildi. E grubuna ise, i.p. olarak likid parafin içinde $CCL_4$ ve standart rat peletine %10 oranında katılmış çörekotu ad libitum verildi. Tüm gruplardan 4 kez, kontrol grubundakilerden bir de deneme başlangıcında olmak üzere 5 kez kan alındı ve bu örneklerde MDA,GSH-Px,SOD,ALT,AST,GSH, serüloplazmin analizleri yapıldı. Biyokimyasal olarak B grubunda lipid peroksidasyon ürünlerinin ve antioksidan enzimlerin belirgin bir şekilde arttığı görüldü. En az % ağırlık kaybı E grubunda gözlendi. Karaciğer örneklerinde en az nekroz oranı D grubunda saptandı. Histopatolojik muayene sonuçları da biyokimyasal sonuçlarla tutarlı idi. Sonuç olarak vitamin E+selenyum ve çörekotunun karaciğer nekrozunu engelleyebildiği kanısına varılmıştır.
Detection of Bovine Respiratory Syncytial Virus, Pasteurella Multocida, and Mannheimia Haemolytica by Immunohistochemical Method in Naturally-Infected Cattle
(Walter de Gruyter Gmbh, 2018) Yaman, Turan; Buyubayram, Huseyin; Ozyildiz, Zafer; Terzi, Funda; Uyar, Ahmet; Keles, Omer Faruk; Yener, Zabit
Introduction: The aim of this study was to determine the predisposing effect of bovine respiratory syncytial virus (BRSV) on Pasteurella spp. infection in naturally-induced pneumonia in cattle by immunohistochemical labelling. Material and Methods: Lungs of cattle slaughtered in the slaughterhouse were examined macroscopically, and 100 pneumonic samples were taken. The samples were fixed in 10% neutral formalin and embedded in paraffin by routine methods. Sections 5 mu m in thickness were cut. The streptavidin-peroxidase method (ABC) was used to stain the sections for immuno-histochemical examination. Results: BRSV antigens were found in the cytoplasm of epithelial cells of bronchi, bronchioles, and alveoles and within inflammatory cell debris and inflammatory exudate in bronchial lumens. Pasteurella spp. antigens were detected in the cytoplasm of the epithelial cells of bronchi and bronchioles, and in cells in the lumens of bronchi and bronchioles. Eleven cases were positive for only one pathogen (six for BRSV and five for Pasteurella spp.), while 35 cases were positive for 2 pathogens: BRSV plus P. multocida (n = 21) or M. haemolytica (n = 14). Conclusion: The presence of high levels of BRSV in dual infections indicates that BSRV may be the main pneumonia-inducing agent and an important predisposing factor for the formation of Pasteurella spp. infections in cattle naturally afflicted with pneumonia.
Effect of Chrysin on Methotrexate-Induced Testicular Damage in Rats
(Wiley, 2019) Belhan, Saadet; Comakli, Selim; Kucukler, Sefa; Gulyuz, Fetih; Yildirim, Serkan; Yener, Zabit
This study was conducted on 28 male Wistar albino rats to determine the effects of chrysin on methotrexate-induced damage to testicular tissue. Rats were grouped into four groups of seven rats reach: Group 1 (n = 7) was the control group to which no drugs were administered; this group was only provided with food and water. Group 2 (n = 7) was administered 20 mg/kg of methotrexate once intraperitoneally. Group 3 (n = 7) was administered 50 mg/kg of chrysin for 7 days orally. Group 4 (n = 7) was administered 20 mg/kg of methotrexate once intraperitoneally, followed by oral administration of 50 mg/kg of chrysin for 7 days. At the end of the experiment, rats were anaesthetised, rat testes were removed, and spermatozoon was obtained from the cauda epididymis. It was determined that sperm count and motility, glutathione peroxidase, superoxide dismutase and catalase activities decreased in the methotrexate group, whereas malondialdehyde, tumour necrosis factor-alpha, interleukin-1 beta and nuclear kappa factor B expression levels increased. Furthermore, damage to tubulus seminiferus structures and affusion in germ cells was identified. In the methotrexate + chrysin administered group, sperm count improved, biochemical enzyme levels increased, and structural improvements were observed in testicular tubules. These findings demonstrated that chrysin plays a protective role in testicular damage in rats.
The Effect of Theranekron on Femur Fracture Healing in an Experimental Rat Model
(Turkish Joint Diseases Foundation, 2022) Guven, Necip; Ozkan, Sezai; Turkozu, Tulin; Koc, Serap; Keles, Omer Faruk; Yener, Zabit; Karasu, Abdullah
Objectives: The aim of this study was to investigate the radiological, biomechanical, histopathological and immunohistochemical effects of Theranekron on fracture healing in an experimental rat model. Materials and methods: Forty-eight male albino Wistar rats were used. Four groups were formed, with 12 rats in each of Theranekron groups 1 and 2, and control groups 1 and 2. After a fracture was created in the right femur of the rats included in the study, fixation was performed with an intramedullary Kirschner wire. Theranekron was administered subcutaneously to Theranekron groups 1 and 2 at a dose of 0.3 mg/kg on days 0, 5 and 10. After radiographic analysis of the femurs of Theranekron group 1 and control group 1 rats at four weeks of the study was performed, both groups were divided into two equal subgroups (six femurs in each group). Histopathological and immunohistochemical examinations were performed in one subgroup and biomechanical examination in the other subgroup. At the end of six weeks, the rats in Theranekron group 2 and control group 2 were evaluated after applying the same procedure as in the fourth week. Results: When the mean radiological scores of the Theranekron and control groups were compared, a statistically significant difference was found in favor of the Theranekron group at four and six weeks (p=0.028 and p=0.006, respectively). At four weeks, statistically significant higher biomechanical forces were obtained in the Theranekron group compared to the control group (p=0.030). In the histopathological evaluation, the inflammation value of the control group at four weeks was statistically significantly higher than the Theranekron group (p=0.027). The angiogenesis, osteoblast proliferation, and bone formation values of the Theranekron group were significantly higher than the control group (p=0.014, p=0.014, and p=0.005, respectively). At six weeks, the bone formation values of the Theranekron group were statistically significantly higher than the control group (p=0.021). The difference between the Theranekron group and the control group scores of the immunohistochemical evaluation were statistically significantly different at four and six weeks (p=0.006 and p=0.011, respectively). Conclusion: Theranekron may play a role in accelerating fracture healing by reducing acute inflammation process in the early period of fracture union, increasing fracture strength, angiogenesis, osteoblast proliferation, and bone formation.
Effectiveness of Hesperidin on Methotrexate-Induced Testicular Toxicity in Rats
(Kafkas Univ, veteriner Fakultesi dergisi, 2017) Belhan, Saadet; Ozkaraca, Mustafa; Kandemir, Fatih Mehmet; Gulyuz, Fetih; Yildirim, Serkan; Omur, Ali Dogan; Yener, Zabit
The aim of this study was to investigate the effect of hesperidin on male reproductive system in rats to which methotrexate (MTX) was administered. In the study, 28 male Wistar albino rats at the age of 8 weeks and had 250-300 g of live weight were used. Four experimental groups were formed; Group 1 (n=7): The control group, only feed and water were given. Group 2 (n=7): MTX group, a single dose of 20 mg/kg of i.p. MTX was administered. Group 3 (n=7): Hesperidin group, 200 mg/kg of hesperidin was administered by gavage for 7 days. Group 4: MTX + hesperidin group (n=7): Following administration of a single dose of 20 mg/kg i.p. MTX, 200 mg/kg of Hesperidin was administered by oral gavage for 7 days. At the end of the experiment, rats were decapitated and biochemical, histopathological and spermatological parameters were examined. It was observed that in the MTX group, sperm motility and density, the enzymes CAT, GPx and SOD and GSH level decreased, TNF-alpha and IL-1 Beta, as well as MDA, levels were increased, regular structure of spermatogenic cells was impaired, and seminiferous tubules became necrotic and degenerative. It was determined that spermatological parameters improved and, necrotic and degenerative changes diminished by the administration of MTX+hesperidin. These outcomes indicated that hesperidin had a protective effect on destructive effects of MTX in rat testiclesv.
The Effects of Dexmedetomidine on Liver Injury in Rats With Experimental Sepsis: a Histopathological and Immunohistochemical Study
(Turkish Assoc Trauma Emergency Surgery, 2025) Keles, Omer Faruk; Kaplan, Havva Sayhan; Cicek, Haci Ahmet; Palabiyik, Onur; Yener, Zabit
BACKGROUND: In the rat sepsis model, the protective effect of dexmedetomidine (Dex) in sepsis-induced tissue injuries by reducing inflammation is still unclear. Research is ongoing to determine whether Dex modulates sepsis-induced tissue injury. The aim of this experimental study was to investigate the effect of Dex on liver injury in sepsis rats histopathologically and immunohistochemically. METHODS: In this study, sepsis was induced in rats by a 10 ml/kg E. coli injection, and the protective efficacy of Dex against liver damage was investigated through histopathological and immunohistochemical findings by the intraperitoneal administration of 100 mu g/ kg Dex. RESULTS: In our results, the most striking and basic morphological changes in the liver tissues of sepsis group rats were neutrophil leukocyte infiltrations in and around the vessels. In Dex-treated groups, neutrophil leukocyte infiltrations were more prominent, and marked dilatations were observed in the vessels. The fact that inflammatory reactions were more prominent in the Dex-treated groups was thought to be related to the increase in vascular permeability due to Dex's vasodilation effect. CONCLUSION: According to the histopathological and immunohistochemical findings obtained in the present study, we conclude that Dex did not alleviate sepsis-induced liver inflammation in a rat sepsis model.
Effects of Urtica Dioica L. Seed on Lipid Peroxidation, Antioxidants and Liver Pathology in Aflatoxin-Induced Tissue Injury in Rats
(Pergamon-elsevier Science Ltd, 2009) Yener, Zabit; Celik, Ismail; Ilhan, Fatma; Bal, Ramazan
This study was carried out to investigate the hepatoprotective and antioxidant properties of Urtica dioica L seeds (LIDS) extract against aflatoxin (AF)-exposure in rats. The preventive potential and antioxidant capacity of the plant's extract was evaluated by liver histopathological changes, measuring serum marker enzymes, antioxidant defense systems and lipid peroxidation (Malondialdehyde, MDA) content in some tissues of rats. Eighteen rats were randomly divided into one of three experimental groups: control, AF-treated group and AF+UDS-treated group. Rats in control group were fed with a diet without AF. Rats in AF-treated group and AF+UDS-treated group received approximately 25 mu gr of AF/rat/day. AF+UDS groups also received 2 mL of LIDS oils/rat/day by gavage for 90 days. Administration of LIDS extract restored the AF-induced imbalance between MDA and antioxidant system towards near normal particularly in liver. Hepatoprotection by LIDS is further substantiated by the almost normal histologic findings in AF+UDS-treated group as against degenerative changes in the AF-treated rats. It is concluded that LIDS has a hepatoprotective effect in rats with aflatoxicosis, probably acting by promoting the antioxidative defense systems. (C) 2008 Elsevier Ltd. All rights reserved.
Examination of Sheep in Which Injection of Yeldif® Induced Sudden Death
(Polish Soc veterinary Sciences Editorial office, 2017) Uyar, Ahmet; Yener, Zabit; Basbugan, Yildiray; Keles, Omer Faruk; Yaman, Turan
Two breeders from the Baskale district of the Van province and the Hizan district of the Bitlis province reported deaths of their lambs one day after injection of a commercial preparation containing a combination of Se and Vit E (Yeldif (R)). Purulent-hemorrhagic-necrotic myositis was detected at the injection site during necropsy of the lambs (2 animals) brought to the Pathology Department of our Faculty by these breeders. This study was conducted to determine whether these post-injection deaths occurred as a result of the drug used or due to a faulty application of the medicine by the breeders. The trial period for this study was 1 week. Eighteen 2-month-old lambs were used in this study. The lambs were divided into three groups (control and 2 study groups) of 6 lambs each. An injection of 1 ml (recommended dose) of the product purchased in the market from the same batch as the one that had caused the deaths was administered I.M. to lambs in the control group. The medicine reported to have caused the deaths by the breeders was administered to the first study group (A) at 1 ml I.M. and to the second study group (B) at 3 ml I.M. (three times the recommended dose). The lambs were bled for biochemical analysis before the administration, on the first day after the administration, and at the end of the trial. Necropsies were performed on dead animals during the trial and on all animals at the end of the trial. At the end of the trial, no clinical-pathological findings were noted in lambs of the control group and group A. However, in all lambs of group B, emphysematous gangrene was detected at the injection site. Cl. chauvoei was isolated and identified in bacteriological and cultural examinations. It was concluded that bacterial contamination occurred as a result of incorrect drug administration by the breeders and injection of a high dose of the contaminated drug.
Histopathologic and Biochemical Investigation of the Preventive Effect of Silymarin on High Fat Diet-Induced Obesity Formation in Rats
(2019) Keleş, İsmail; Yener, Zabit; Huyut, Zübeyir
Keleş İ. Ratlarda Yüksek Yağlı Diyet ile indüklenen Obezite Oluşumu Üzerine Silymarin'in Engelleyici Etkisinin Histopatolojik ve Biyokimyasal Olarak Araştırılması. Van Yüzüncü Yıl Üniversitesi, Sağlık Bilimleri Enstitüsü, Patoloji Anabilim Dalı Yüksek Lisans Tezi, Van, 2019. Bu çalışmada, yüksek yağlı diyetle beslenerek kilo artışı sağlanan ratlarda, Silymarin'in obezite üzerine etkisi histopatolojik ve biyokimyasal olarak araştırıldı. Bu amaçla 32 rat, her bir grupta sekiz rat olacak şekilde; Kontrol (n:8), Obezite (n:8), Obezite+Silymarin (n:8) ve Silymarin (n:8) olmak üzere rastgele 4 gruba ayrıldı. Kontrol grubu, standart rat pelet yemiyle beslendi. Obezite grubu, enerji değeri yüksek yağ diyetli pelet yem ile beslendi. Obezite+Silymarin grubu, enerji değeri yüksek diyetli pelet yem ile beslenmeye ilaveten Silymarin ratlara 3mg/kg/rat dozda günlük olarak orogastrik gavaj ile verildi. Silymarin gurubu ise; standart pelet yem ile beslenmeye ilaveten Silymarin ratlara 3mg/kg/rat dozda günlük olarak orogastrik gavaj ile verildi. Çalışma boyunca sıçanların kilo alımı takip edildi. Deneme sonucunda nekropsi yapılan ratlardan biyokimyasal ve histopatolojik incelemeler için kan ve doku örnekleri alındı. Histopatolojik olarak obezite grubunda; karaciğerde sentrilobuler hepatositlerde farklı büyüklüklerde keskin sınırlı yağ vakuolleri ve bu hepatositlerin kimisinde koagulasyon nekrozu gözlenirken, Obezite+Silymarin grubunda kontrol grubunda olduğu gibi karaciğerin normal histolojik görünümü izlendi. Kan serumu örneklerinde; alanin transaminaz (ALT), aspartik transaminaz (AST), alkalen fosfataz (ALP), laktat dehidrogenaz (LDH), gama glutamil transferaz (GGT), kolesterol, trigliserit, HDL kolesterol, LDL kolesterol ve açlık kan şekeri (AKŞ) aktiviteleri belirlendi. Obezite grubundaki ALP, LDH, trigliserit ve AKŞ aktiviteleri kontrol grubuna göre önemli ölçüde arttığı, obezite+silymarin grubunda ise kontrol grubuna yakın olduğu tespit edildi. Sonuç olarak, enerji değeri yüksek diyetli pelet yem ile beslenmeye ilaveten Silymarin verilen ratlarda karaciğer yağlanmasının büyük oranda önlendiği belirlendi.
Histopathologic and Immunohistochemical Investigation of the Therapeutic Effect of Stinging Nettle Seed (urtica Dioica Seed) in Ethanol-Induced Liver Fibrosis in Rats
(2019) Yardımcı, Mahmut; Yener, Zabit
Yardımcı M, Ratlarda Etanol İle Oluşturulmuş Karaciğer Fibrozisinde Isırgan Otu Tohumunun (Urtica dioica Seed) Tedavi Edici Etkisinin Histopatolojik ve İmmunohistokimyasal Olarak Araştırılması, Van Yüzüncü Yıl Üniversitesi, Sağlık Bilimleri Enstitüsü, Patoloji Ana Bilim Dalı, Yüksek Lisans Tezi, Van, 2019. Planlanan bu çalışmada, etil alkol ile deneysel olarak 8 haftalık süre içinde karaciğerde fibrozis oluşturulduktan sonraki 2 aylık tedavi sürecinde, ısırgan otu tohumu ekstrakt'ı kullanılarak fibrozisin geri dönüşümünün mümkün olup olmadığı histopatolojik ve immünohistokimyasal olarak araştırıldı. Çalışmada toplam 24 adet rat kullanıldı ve ratlar her grupta 8'er adet olmak üzere üç gruba ayrıldı. Deneme süresi toplam 4 ay olarak belirlendi. Birinci grup (Kontrol); Standart rat pelet yemiyle beslendi. Alkol grubu ve alkol+ısırgan tohumu esktrat grubunda ise denemenin ilk 2 ayında günde bir defa orogastrık gavajla 4 mL/kg %30'luk etil alkol verilerek hepatik fibrozis oluşturuldu. Daha sonraki 3. ve 4. aylarda 2. gruba yine sadece alkol verilirken, 3. grup ratlara alkol alımıyla beraber ısırgan otu tohumu ekstrakt'ı (30mg/kg)'da verilerek oluşturulan fibrozisin geri dönüşümü uygulama sonunda izlendi. Deneme sonunda nekropsi yapılan ratların karaciğerlerinden alınan doku örnekleri histopatolojik ve immünohistokimyasal olarak incelendi. Sonuçta, histopatolojik ve immünohistokimyasal bulgular doğrultusunda; ısırgan tohumu ekstrakt'ının kaydedilen birçok terapötik özelliklerinin etkisiyle ve karaciğerin de rejeneratif kapasitesi sayesinde karaciğerdeki dejeneratif-nekrotik değişikliklerin ve fibrozisin önemli düzeylerde geri dönüşümünün gerçekleştiği gözlenmiştir. Biyokimyasal analizler Alkol grubunda ALT, AST, LDL, GGT ve HDL düzeyleri kontrol grubuna göre azalmıştı. Ancak, Glukoz, LDL, Total Kolesterol ve Trigliserid seviyeleri kontrol grubuna göre artmıştı. Alkol+Isırgan grubunda ise kontrol grubuna göre ALT, AST, LDH, Glukoz, LDL ve Trigliserid düzeyleri azalmıştı. Ancak AlkPaz, GGT, HDL ve Total Kolesterol seviyeleri kontrol grubuna göre artmıştı. Çalışmanın sonuçları, alkolik hepatik fibrozisli olgularda, ısırgan otu tohumu ekstrakt'ının fibrozisli karaciğer tedavisinde kullanılabileceğini gösterdi.
Histopathological and Biochemical Investigation of the Preventive Effects of Broiler Feeds Including Urtica Dioica Seed Extract Against Aflatoxicosis
(2014) Uyar, Ahmet; Yener, Zabit
ÖZET Uyar A, Broyler Yemlerine Katılan Isırgan Otu Tohumu (Urtica Dioica Seed) Ekstraktının Aflatoksikozise Karşı Koruyucu Etkinliğinin Histopatolojik ve Biyokimyasal Olarak Araştırılması, Y.Y.Ü. Sağlık Bilimleri Enstitüsü, Patoloji Anabilim Dalı Doktora Tezi, Van, 2014. Aflatoksikozis, insan ve hayvanlarda aflatoksinler tarafından oluşturulan bir mikotoksikozisdir. Hayvanlarda önemli ekonomik kayıplara neden olan ve insan sağlığını tehdit eden toksikasyon özellikle geri kalmış ve gelişmekte olan ülkelerde yaygın olarak görülmektedir. Yapılan son çalışmalardan ısırgan otunun immunomodülatör, antioksidan ve antikanserojenik gibi birçok farmakolojik etkilerinin olduğu bilinmektedir. Ancak ısırgan otu tohumu eksraktının aflatoksikozise karşı koruyucu etkileri konusunda broyler yetiştiriciğinde çalışma bulunmamaktadır. Gerçekleştirilen bu çalışmada, broylerlerde deneysel olarak oluşturulan aflatoksikozis'de karaciğer ve böbreklerde meydana gelen lezyonların oluşumunda ısırgan otu tohumu ektraktının engelleyici etkisi; histopatolojik ve biyokimyasal olarak araştırıldı. Bu amaçla, toplam 32 broyler civciv, herbiri 8'erli 4 gruba ayrıldı. Deneme grupları; kontrol grubu (A); normal broyler yemi, IT grubu (B); 30 ml/kg yeme ısırgan otu tohumu ekstresi, AF grubu (C); 1mg/kg AF, AF+IT grubu (D); 1mg/kg AF + 30 ml/kg dozda ısırgan otu tohumu ekstresi ilaveli yemler ile beslendi. Yirmibir günlük deneme sonunda nekropsileri yapılan broylerlerden biyokimyasal ve histopatolojik analizler için kan ve doku örnekleri alındı. Biyokimyasal bulgulara göre sadece IT ekstraksiyonu verilen deneme grubundaki değerlerin kontrol grubuyla benzer olduğu, AF grubunda lipid pereksidasyonun ve karaciğer enzimlerinin önemli ölçüde yükseldiği ve antioksidan savunma sistemlerinin de azaldığı, AF+IT grubunda ise lipid pereksidasyonun ve karaciğer enzimlerinin azaldığı ve kontrol grubuna yaklaştığı, antioksidan sistem aktivitesinin de arttığı gözlendi. Patolojik olarak da C grubunda (AF) saptanan karaciğer ve böbreklerdeki büyüme-solgunluk gibi makroskobik değişiklikler ile hidropik dejenerasyon, safra kanalı proliferasyonu, periportal fibrozis ve perisinüzoidal hücrelerde aktivasyon gibi histolojik bozuklukların IT ekstraktı ilave edilen D grubunda (AF+IT) önemli ölçüde azaldığı ya da oluşmadığı gözlendi. Bu bulgular doğrultusunda broyler aflatoksikoziste, IT ekstraktının lipid peroksidasyonu ve doku hasarını önemli ölçüde engellediği, dolaysıyla koruyucu etkiye sahip olduğu kanısına varıldı. Anahtar sözcükler: Aflatoksikozis, Isırgan otu tohumu ekstresi, Broyler
Histopathological and Biochemical Investigations of Effect of Grape Speed Extaction on the Prevention of Aflatoxin-Induced Liver Lesions in Rats
(2009) Ürkek, Tuba Aktaş; Yener, Zabit
Aflatoksikozis, insan ve hayvanlarda aflatoksinler tarafından oluşturulan akut veya kronik seyirli bir mikotoksikozisdir. Hayvanlarda önemli ekonomik kayıplara neden olan ve insan sağlığını tehdit eden toksikasyon özellikle geri kalmış ve gelişmekte olan ülkelerde yaygın olarak görülmektedir. Kronik zehirlenmeler, hayvan ve insanlarda özellikle bağışıklık sisteminin baskılanmasına yol açarak birçok hastalığın ön plana çıkmasına sebep olmaktadır. Aflatoksinler, özellikle hepatotoksik ve hepatokarsinojenik etkileriyle bilinmektedir. Aflatoksinlerden ileri gelen toksikasyonlarda etkili bir korunma bilinmemektedir. Ancak bazı vitamin ve mineraller ile proteinlerin koruyucu etkilerinin olduğu belirtilmektedir. Yapılan son çalışmalardan üzüm çekirdeğinin immunomodülatör, antioksidan ve antikanserojenik gibi birçok farmakolojik etkilerinin olduğu bilinmektedir. Ancak üzüm çekirdeğinin aflatoksikozise karşı hepatoprotektif etkileri konusunda çalışma bulunmamaktadır. Gerçekleştirilen bu çalışmada, ratlarda deneysel olarak oluşturulan aflatoksikozis'de karaciğerde meydana gelen kronik lezyonların oluşumunda, üzüm çekirdeğinin engelleyici etkisi; biyokimyasal ve histopatolojik olarak araştırıldı. Bu amaçla, 30 rat, 10'arlı 3 grub'a ayrıldı. Çalışma grubu olarak belirlenen ratlara aflatoksin, aflatoksinle birlikte üzüm çekirdeği ekstraktı verildi. Üç aylık deneme sonunda nekropsileri yapılan ratlardan kan ve doku örnekleri alınarak biyokimyasal ve patolojik olarak incelendi. Biyokimyasal ve histopatolojik bulgulara göre lipid peroksidasyonun ve karaciğer hasarının önemli ölçüde engellendiği, dolaysıyla ÜÇ ekstraktının hepatoprotektif etkiye sahip olduğu saptandı.
Histopathological and Biochemical Investigations of Protective Role of Honey in Rats With Experimental Aflatoxicosis
(Bmc, 2016) Yaman, Turan; Yener, Zabit; Celik, Ismail
Background: Natural honey (honey) is considered as a part of traditional medicine all over the world. It has both antimicrobial and antioxidant properties, useful in stimulation of wounds and burns healing and gastric ulcers treatment. The aim of this study, for the first time, was to investigate the antioxidant properties and protective role of honey against carcinogen chemical aflatoxin (AF) exposure in rats, which were evaluated by histopathological changes in liver and kidney, measuring level of serum marker enzymes [aspartate aminotransferase (AST), alanin aminotransferase (ALT), gamma glutamil transpeptidase (GGT)], antioxidant defense systems [Reduced glutathione (GSH), glutathione reductase (GR), superoxide dismutase (SOD), glutathione-S-transferase (GST) and catalase (CAT)], and lipid peroxidation content in liver, erythrocyte, brain, kidney, heart and lungs. Methods: Eighteen healthy Sprague-Dawley rats were randomly allocated into three experimental groups: A (Control), B (AF-treated) and C (AF + honey-treated). While rats in group A were fed with a diet without AF, B, and C groups received 25 mu g of AF/rat/day, where C group additionally received 1 mL/kg of honey by gavage for 90 days. Results: At the end of the 90-day experimental period, we found that the honey supplementation decreased the lipid peroxidation and the levels of enzyme associated with liver damage, increased enzymatic and non-enzymatic antioxidants in the AF + honey-treated rats. Hepatoprotective and nephroprotective effects of honey is further substantiated by showing almost normal histological architecture in AF + honey-treated group, compared to degenerative changes in the liver and kidney of AF-treated rats. Additionally, honey supplementation ameliorated antioxidant defens systems and lipid peroxidation in content in other tissues of AF + honey treated rats. Conclusion: The present study indicates that honey has a hepatoprotective and nephroprotective effect in rats with experimental aflatoxicosis due to its antioxidant activity.
Histopathological and Genetical Investigation of the Protective Efficiency of Polygonum Aviculare Extract on Experimentally Azoxymethane-Induced Colonic Aberrant Crypt Formation in Rats.
(2021) İncecik, Veli; Yener, Zabit; Arslan, Mevlüt
Bu çalışmada, kanserojen bir madde olan azoksimetan (AOM) ile indüklenen kolon kanseri modelinde fenolik bileşiklerce zengin olan Polygonum aviculare (PA) ekstresinin kemopreventif etkisi araştırıldı. Bu amaçla 32 adet erkek Wistar albino rat; kontrol, AOM, AOM + PA ve PA grupları olmak üzere dört gruba ayrıldı. Kontrol grubu standart pelet yem ile beslenirken, AOM ve AOM + PA gruplarına 4 hafta boyunca iki haftada bir kez 15 mg/kg AOM subkutan olarak uygulandı. AOM + PA ve PA gruplarına PA ekstresi 3 ml/kg dozda orogastrik olarak verildi. 16 haftalık çalışma süresi sonunda analizler için ratlardan kan ve nekropsi sonrası doku örnekleri alındı. Histopatolojik olarak kolon dokusunda adenom ve adenokarsiom oluşumları, immunohistokimyasal olarak CEA ve COX-2 ekspresyonları değerlendirildi. Biyokimyasal olarak serumda rutin biyokimya parametreleri ölçülürken serum ve doku örneklerinde Bax, BcL-2, kaspaz 3, TOS, TAS, Süperoksit dismutaz (SOD), Glutasyon (GSH), Katalaz (CAT), AQP-1 ve AQP-7 seviyeleri ile ayrıca serum kolorektal kanser biyobelirteçleri olan CEA, CA 19-9, CA 125 ve CA-15-3 düzeyleri değerlendirildi. Genetik olarak kolon dokularında pro-inflamatuar sitokinler (IL-6, IL-1β, TNF-α), inflamasyon ilişkili enzimler (COX-2, iNOS), proliferasyon belirteci (PCNA) ve anti-apoptotik (Bcl-2) genlerinin mRNA ekspresyon düzeyleri araştırıldı. Sonuçlar, PA ekstresinin oral uygulamasının, AOM'nin neden olduğu kolorektal karsinogenezisi, adenom ve adenokarsinomların sayı ve boyutlarında azalmayla önemli ölçüde inhibe ettiğini gösterdi. İlaveten, PA ekstresi uygulamasının kolon dokusunda oksidatif stres üzerine inhibitör etki gösterirken, antioksidan aktiviteler üzerine stimülatör etki ortaya koydu. Diğer bazı dokularda ise bu değerlerde dalgalanma gözlendi. PA ekstresi uygulamasının sonucu CEA ve COX-2 immun-ekspresyonlarında azalma, kaspaz-3 immunoreaksiyonunda ise artış meydana geldi. PA ekstresi pro-inflamatuar sitokinlerin, inflamasyon ilişkili genlerin mRNA sevisiyesini düşürdü, anti-apoptotik genin ekspresyonunu baskıladı ve PCNA geni mRNA seviyesini düşürdü. Bu sonuçlara göre, PA ekstresi uygulamasının hücre proliferasyonunu, pro-inflamasyon sitokinlerinin ve COX-2 ekspresyonunu inhibe ederek ve apoptozu indükleyerek kolon tümör odaklarında anlamlı sayısal azalmaya sebep olduğundan, bitkinin kemopreventif etkili bir diyet ajanı olabileceği kanısına varılmıştır.
Histopathological and Immunohistochemical Investigation of the Preventive Effect of Nigella Sativa Exctrat on Obesity Induced by High Fat Diet in Rats.
(2019) Işık, Maksut; Yener, Zabit
Bu çalışmada, yüksek yağlı diyetle beslenerek kilo artışı sağlanan ratlarda, Nigella sativa'nın (NS) obezite üzerine etkisi histopatolojik ve biyokimyasal olarak araştırıldı. Bu amaçla 32 rat, her bir grupta sekiz rat olacak şekilde; Kontrol (n:8), Obezite (n:8), Obezite+NS (n:8) ve NS (n:8) olmak üzere rastgele 4 gruba ayrıldı. Kontrol grubu, standart rat pelet yemiyle beslendi. Obezite grubu, enerji değeri yüksek yağ diyetli pelet yem ile beslendi. Obezite+NS grubu, enerji değeri yüksek diyetli pelet yem ile beslenmeye ilaveten NS ekstresi ratlara 3mg/kg/rat dozda günlük olarak orogastrik gavaj ile verildi. NS gurubu ise; standart pelet yem ile beslenmeye ilaveten NS ekstresi ratlara 3mg/kg/rat dozda günlük olarak orogastrik gavaj ile verildi. Çalışma boyunca sıçanların kilo alımı takip edildi. Deneme sonucunda nekropsi yapılan ratlardan biyokimyasal ve histopatolojik incelemeler için kan ve doku örnekleri alındı. Histopatolojik olarak obezite grubunda; karaciğerde sentrilobuler hepatositlerde farklı büyüklüklerde keskin sınırlı yağ vakuolleri ve bu hepatositlerde koagulasyon nekrozu gözlenirken, Obezite+NS ekstresi grubunda kontrol grubunda olduğu gibi karaciğerin normal histolojik görünümü izlendi. Kan serumu örneklerinde; alanin transaminaz (ALT), aspartik transaminaz (AST), alkalen fosfataz (ALP), laktat dehidrogenaz (LDH), gama glutamil transferaz (GGT), kolesterol, trigliserit, HDL kolesterol, LDL kolesterol ve açlık kan şekeri (AKŞ) aktiviteleri belirlendi. Obezite grubunda trigliserid düzeyi; kontrol, NS+obezite ve NS grubuna göre anlamlı olarak yüksek bulundu. NS+obezite grubunda serum ALT düzeyi; kontrol ve obezite grubundan anlamlı olarak daha yüksekti. ALP, LDH, trigliserit ve AKŞ aktiviteleri kontrol grubuna göre önemli ölçüde arttığı, obezite+NS ekstresi grubunda ise kontrol grubuna oldukça yakın olduğu tespit edildi. Sonuç olarak, enerji değeri yüksek diyetli pelet yem ile beslenmeye ilaveten NS ekstresi verilen ratlarda karaciğerde yağ dejenerasyonun büyük oranda önlendiği belirlendi.