Browsing by Author "Yildirim, M."

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Anti-Inflammatory Effects of Usnic Acid in Breast Cancer
(Maik Nauka/interperiodica/springer, 2022) Yildirim, M.; Degirmenci, U.; Akkapulu, M.; Gungor, M.; Oztornaci, R. O.; Berkoz, M.; Yalin, S.
cancer is the most common type of cancer among women. Usnic acid has anticancer and anti-inflammatory potential. In this study, we evaluated the effects of usnic acid on inflammation and oxidative stress in the breast cancer cell line (MCF-7). Cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) gene expression, cytokines, nitric oxide (NO), prostaglandin E2 (PGE2) levels and oxidative stress were measured in all groups. Our study showed that usnic acid was decreased NO, VEGF, PGE2 levels, gene expression levels of COX-2 and iNOS in the meantime cytokines (IL 2, CXCL 10, CXCL8, CCL2 (MCP-1), TNF-alpha, IL-6), glutathione levels, moreover decreased GSH and increased MDA levels in dose depended manner. According to our evidence usnic acid was showed potential cytotoxic, anti-inflammatory and pro oxidant role.
Effect of Capsaicin on Transcription Factors in 3t3-L1 Cell Line
(Yuzuncu Yil Universitesi Tip Fakultesi, 2015) Berkoz, M.; Yildirim, M.; Arvas, G.; Turkmen, O.; Allahverdiyev, O.
Capsaicin is a spicy ingredient of Capsicum annuum and a lipophilic, crystalline, odorless and colorless alkaloid. Although the effect of capsaicin on adipocyte differentiation is well-known, the role of capsaicin on transcription factors while adipocyte differentiation is not clear. The aim of this study is thus to identify and characterize the transcription factors in the process of adipocyte differentiation after the capsaicin treatment. In this study, concentration of 0, 50, 100, 150, 200 and 250 µM capsaicin were treated to 3T3-L1 pre-adipocytes in cell culture. MTT cell cytotoxicity, cell viability with trypan blue staining, Lactate Dehydrogenase (LDH) enzyme assay, triglyceride content assay, Glycerol-3-Phosphate Dehydrogenase (GPDH) activity, Oil Red O staining and mRNA levels of transcription factors (PPARγ, C/EBPα and SREBP-lc) were investigated in capsaicin induced 3T3L1 preadipocyte cell line. Capsaicin treatment decreased cell population growth of 3T3-L1 preadipocytes, assessed with trypan blue staining, MTT test and rising of LDH release proportion. Capsaicin inhibited GPDH activity and intracellular triglyceride content in 3T3-L1 adipocytes in all treated groups in a dose-dependent manner. Oil Red O staining indicated that capsaicin inhibited adipocyte differentiation in 3T3-L1 adipocytes in all treatment groups. In this study, it was revealed that exposing 3T3-L1 preadipocytes and differentiating postconfluent preadipocytes to different doses of capsaicin decreased PPARγ, C/EBPα and SREBP-1c mRNA levels as compared with their controls without treatment in dose dependent manner. Although, reduction of PPARγ mRNA level was statistical significant, this decrease was not significant in C/EBPα and SREBP-1c mRNA levels. This study demonstrated that capsaicin treatment inhibited the adipogenesis through the down-regulation of transcription factors, especially PPARγ. Alternative mechanisms may involve cell cycle arrest and the induction of apoptosis. Since capsaicin is the main component found in hot pepper, the consumption of hot pepper may contribute to the maintenance of body weight and prevent the development of obesity. © 2015 Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
The Effect of Rheum Ribes L. on Oxidative Stress in Diabetic Rats
(De Gruyter Open Ltd, 2021) Yildirim, M.; Degirmenci, U.; Akkapulu, M.; Comelekoglu, U.; Balli, E.; Metin Ozcan, T.; Yalin, S.
Rheum ribes L. is a perennial plant that belongs to the family of Polygonaceae, which is often used in traditional therapy because it possesses many bioactivities, such as antioxidant and antibacterial ones. Here we examined the effect of different R. ribes L. extracts on oxidative stress in experimental diabetic rats. Thirty-six rats were divided into six groups as follows: group I, control group; group II, diabetic rats; group III, diabetic rats treated with the aqueous extract of R. ribes L. by gavage at 50 mg/kg for 15 days; group IV, diabetic rats treated by gavage with the ethanolic extract of R. ribes L. at 50 mg/kg for 15 days; group V, nondiabetic rats treated by gavage with the aqueous extract of R. ribes L. at 50 mg/kg for 15 days; group VI, nondiabetic rats treated by gavage with the ethanol extract of R. ribes L. at 50 mg/kg for 15 days. After 15 days, the animals were sacrificed and the liver and kidney tissues of each animal were isolated. Superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) activities in the tissue samples were measured, and histopathologic examination was carried out. R. ribes L. was effective in reducing the oxidative stress and increasing the levels of the antioxidant enzymes. Increased levels of MDA and decreased levels of SOD, CAT and GSH-Px were observed in both the liver and kidney tissues in group II. Decreased levels of MDA and increased levels of SOD, CAT and GSH-Px were observed in group III compared with group II. In group IV, decreased levels of MDA and increased levels of SOD, CAT and GSH-Px were observed in comparison with group II. Diabetes increases oxidative stress and causes a decrease in antioxidant enzyme levels. Both aqueous and ethanolic extracts of R. ribes L. decrease oxidative stress activity and increase the levels of antioxidant enzymes. The ethanol extract of R. ribes L. has a higher antioxidant effect than the aqueous extract. © 2020 2020 Walter de Gruyter GmbH, Berlin/Boston.
The Effect of Sitagliptin and Exenatide on Insulin, Insulin Receptor and Insulin Receptor Substrate Gene Expression in Diabetic Rats
(Maik Nauka/interperiodica/springer, 2023) Yildirim, M.; Mistik, O. Agtas; Yalin, A. E.; Comelekoglu, U.; Berkoz, M.; Arpaci, R. Bozdogan; Yalin, S.
Diabetes mellitus is a chronic metabolic disorder characterized by persistent hyperglycemia that results from abnormal secretion of insulin, inappropriate action of insulin or both. The aim of this study is to determine the effects of exenatide and sitagliptin treatment on the expression of insulin (INS-1), insulin receptor (INSR) and insulin receptor substrate (IRS-1) genes. In this study streptozotocin-induced diabetic rats were treated with sitagliptin (10 mg/kg) and exenatide (0.1 mu g/kg) for 15 days. We measured INS-1, INSR and IRS-1 gene expression levels and examined the histology of pancreatic tissues in all groups. Exenatide and sitagliptin treatment increased gene expression levels of INS-1, INSR and IRS-1.
Increased Oxidative Stress in Living Kidney Donors: Correlation of Renal Functions With Antioxidant Capacity
(Elsevier Science inc, 2017) Yildirim, M.; Karahan, M.; Kucuk, H. F.; Demir, T.; Demir, H.; Turan, H.; Ari, E.
Background. Substantial attention has recently been paid to the possibility of an increased risk of chronic kidney disease (CKD) in living kidney donors. It has been demonstrated that CKD patients suffer from increased oxidative stress, which have been reported to show a strong association with several clinical problems such as accelerated atherosclerosis. The purpose of the current cross-sectional, single-center study was to evaluate the relationship between renal functions of living kidney donors and systemic oxidative stress. Methods. A total of 55 living kidney donors operated at least 1 year ago and 40 age and sex-matched healthy individuals were enrolled in this study. Plasma malondialdehyde (MDA) levels were determined as oxidative stress marker. Superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities were measured as antioxidants. Renal function parameters and proteinuria were also assessed. Results. Mean serum creatinine levels were higher (P = .022) and 24-hour creatinine clearance was lower (P = .016) in living kidney donors compared with healthy controls. Serum MDA levels were higher (P = .034), and SOD and GPx activities were lower (P = .023 and P < .001, respectively). There was a significant positive correlation between serum GPx activity and 24-hour creatinine clearance levels (r = 0.524, P < .01). A linear regression analysis showed that serum GPx activity was still significantly and positively correlated with creatinine clearance (regression coefficient = 0.416, P < .001). Conclusion. Our data demonstrated that living kidney donors exhibit slightly reduced kidney function, increased oxidative stress, and decreased antioxidant activity. We propose that 24-hour creatinine clearance is positively correlated with antioxidant enzyme GPx. To our knowledge, this is the first study to demonstrate the association between renal functions and antioxidant activity in kidney donors.
Oxidative Dna Damage Is Increased in Living Kidney Donors
(Elsevier Science inc, 2019) Karahan, M.; Yildirim, M.; Kucuk, H. F.; Turunc, V; Demir, H.; Salturk, C.; Ari, E.
Background. Long-term consequences of donor nephrectomy might be reduced kidney function, increased risk for cardiovascular disease, and impaired quality of life. The purpose of the current cross-sectional study was to evaluate the relationship between clinical, laboratory, and donation-specific outcomes of living kidney donors and systemic oxidative DNA damage. Methods. We conducted a cross-sectional study and assessed retrospectively pre- and postdonation data from 60 donors who donated between 2010 and 2015. Plasma malondialdehyde levels and 8-hydroxy-20-deoxyguanosine/deoxyguanosine ratio (8-OHdG/dG ratio) were determined as oxidative stress markers. Catalase, carbonic anhydrase, and paraoxonase (PON) activities were measured as antioxidants. Results. Approximately 3 years after donation, the hypertensive donor ratio was 12%, and 11% of the donors had glomerular filtration rate <60 mL/min/1.73 m(2). Mean serum urea (P =.001) and serum creatinine levels (P =.001) were increased; creatinine clearance level (126.2 +/- 35.5 vs 94.6 +/- 26.8, P =.001) was decreased in the postdonation period. There was a significant positive correlation between predonation serum urea and 8-0HdG/dG ratio (r = 0.338, P =.016) and predonation serum creatinine and 8-0HdG/dG ratio (r = 0.442, P =.001), while there was a significant negative correlation between serum creatinine and PON activity (r = -0.545, P <.001). Conclusion. Our data have demonstrated that kidney donors exhibit increased oxidative DNA damage and decreased antioxidant activity. We propose that predonation serum creatinine is positively correlated with 8-0HdG/dG ratio and negatively correlated with antioxidant PON activity. This is the first study to demonstrate that plasma oxidative DNA damage increases in healthy kidney donors.
Pai-1 and Tpa Gene Polymorphisms and Susceptibility To Chronic Obstructive Pulmonary Disease in a Sample of Turkish Population
(Pakistan Medical Association, 2020) Sunnetcioglu, A.; Berkoz, M.; Yildirim, M.; Bora, G.; Yalin, S.
Objective: The aim of this study was to assess the influence of plasminogen activator inhibitor-1 (PAI-1) 4G/5G or tissue plasminogen activator (tPA) I/D polymorphisms in chronic obstructive pulmonary disease (COPD) cases in a sample of Turkish population. Methods: PAI-1 4G/5G and tPA Alu-repeat I/D genetic polymorphisms in 153 COPD subjects and 160 controls were investigated using PCR-RFLP and PCR methods, respectively. Results: 4G allele frequency was 0.62 and 0.39 for COPD and control groups, respectively. 4G allele had an estimated 2.56-fold [95% CI = 1.85-3.53] increased risk of COPD. tPA I allele frequency was 0.55 and 0.50, for COPD and control groups, respectively. I allele had an estimated 1.19-fold [95% CI = 0.87-1.62] increased risk of COPD Conclusions: PAI-1 4G/4G and 4G/5G genotypes seemed to play a key role in the pathophysiology of COPD in Turkish individuals. © 2020 Pakistan Medical Association. All rights reserved.
Punicalagin and Punicalin Suppress the Adipocyte Differentiation Through the Transcription Factors
(Editura Acad Romane, 2021) Berkoz, M.; Yalin, S.; Yildirim, M.; Yalin, A. E.; Comelekoglu, U.
Background. Pomegranate is a rich source of many polyphenolic compounds including ellagitannins (punicalagin, punicalin and others). Aim. The effects of punicalagin and punicalin on adipogenesis were investigated in this study. Materials and Methods. To examine the effect of punicalagin and punicalin on adipocyte differentiation, various concentrations of punicalagin and punicalin (2-10 mu M) were applied to differentiated 3T3-L1 cells. Glyceraldehyde-3-phosphate dehydrogenase (GPDH) activity, Oil red O staining, intracellular triglyceride levels, and gene expressions of transcription factors (Peroxisome proliferator-activated receptor-gamma (PPAR gamma), CCAAT-enhancer-binding proteins-alpha (C/EBP alpha), Sterol regulatory element-binding protein 1c (SREBP-1c)) and lipolysisassociated genes (hormone-sensitive lipase (HSL), Perilipin A, tumor necrosis factor-alpha (TNF-alpha)) were examined in order to investigate the effects of punicalagin and punicalin on adipocyte differentiation. Results. Punicalagin and punicalin applications caused a continuous decrease in cell size and intracellular triglyceride accumulation. GPDH activity and transcription gene expressions decreased significantly in groups that were applicated punicalagin and punicalin at high concentrations. Punicalagin, but not punicalin, down-regulated the expression of HSL and perilipin A and up-regulated the expression of TNF-alpha in a dose-dependent manner. In conclusion, both punicalagin and punicalin were able to inhibit the adipocyte differentiation.
Treatment Options in Primary Mediastinal B Cell Lymphoma Patients, Retrospective Multicentric Analysis; a Turkish Oncology Group Study
(Wolters Kluwer Medknow Publications, 2023) Acar, R.; Paydaş, S.; Yildirim, M.; Kiliçarslan, E.; Sahin, U.; Dogan, A.; Karadurmus, N.
Introduction and Aim: Primary mediastinal B-cell lymphomas (PMBL) are aggressive B- cell lymphomas. Although the initial treatment models vary in PMBL, appropriate treatment methods are not known. We aim to show real-life data on health outcomes in adult patients with PMBL who received various type of chemoimmunotherapies in Turkey. Method: We analyzed the data of 61 patients who received treatments for PMBL from 2010 to 2020. The overall response rate (ORR), overall survival (OS) and progression-free survival (PFS) of the patients were evaluated. Results: 61 patients were observed in this study. The mean age of the study group was 38.4 ± 13.5 years. From among them, 49.2% of the patients were female (n = 30). For first-line therapy, 33 of them had received rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisone (R-CHOP) regimen (54%). Twenty-five patients had received rituximab, etoposide, prednisone, vincristine, cyclophosphamide and doxorubicin (DA-EPOCH-R) regimen. The ORR was 77%. The median OS and PFS were as follows: 25 months (95% CI: 20.4-29.4) and 13 months (95% CI: 8.6-17.3), respectively. The OS and PFS at 12 months were 91.3% and 50%, respectively. The OS and PFS at five years were 64.9% and 36.7%, respectively. Median follow-up time period was 20 months (IQR 8.5-38.5). Conclusion: R-CHOP and DA-EPOCH-R showed good results in PMBL. These remain one of the best determined systemic treatment options for first-line therapy. Also, the treatment was associated with good efficacy and tolerability. © 2022 Journal of Cancer Research and Therapeutics | Published by Wolters Kluwer - Medknow.