Browsing by Author "Yildirim, Metin"

Now showing 1 - 9 of 9

Can Juniperus Communis L. Oil Improve Nephropathy in Diabetic Rats
(Marmara Univ, inst Health Sciences, 2020) Kahraman, Tahir; Berkoz, Mehmet; Allahverdiyev, Oruc; Mahmood, Evan Abdulkarim; Yildirim, Metin; Yalin, Serap
Objective: Juniperus communis L. (J. communis) is a shrub belonging to family Cupressaceae L. mainly growth in Eurasia. The antioxidant and antidiabetic activity of aqueous extract of J. communis L. berries indicated benefits as a potent antidiabetic in streptozotocin induced diabetic albino rats. This study was carried out to determine whether J. communis L. oil supplement will effectively manage renal dysfunction in diabetic rats. Methods: Twenty eight rats were divided into 4 equal groups as follows; control group, diabetic group (45 mg/kg, i.p. streptozotocin), J. communis L. oil (200 mg/kg) treated group, and diabetic+J. communis L. oil (200 mg/kg) treated group. At the end of the experimental period, all rats were sacrificed and renal function parameters such as kidney antioxidant and lipid peroxidation markers and serum glucose, HbA1c, creatinine, serum urea, blood urea nitrogen (BUN), and serum total protein levels were measured in all groups. Results: HbA1c, serum glucose, urea, creatinine, BUN and, kidney lipid peroxidation levels increased (p<0.05), but serum total protein and antioxidant levels decreased in diabetic group comparing with control group (p<0.05). Furthermore, HbA1c, serum glucose, urea, creatinine and BUN and, kidney lipid peroxidation levels decreased and also, serum total protein and antioxidant levels increased in diabetic group treated with J. communis L. oil comparing with diabetic group (p<0.05). Conclusion: This study has provided that J. communis L. oil provide a protective effect on the kidney as evidenced by an improvement of the renal function tests as well as reduction in oxidative stress parameters in experimental diabetic nephropathy model.
Effects of Fenbutatin Oxide on Antioxidant System of Different Tissues in Cyprinus Carpio (L., 1758)
(Parlar Scientific Publications (p S P), 2017) Ozkan-Yilmaz, Ferbal; Ozluer-Hunt, Arzu; Gunduz, Suna Gul; Berkoz, Mehmet; Yalin, Serap; Yildirim, Metin
In this study, effects of sublethal concentrations of fenbutatin oxide on catalase (CAT) and superoxide dismutase (SOD) activities and lipid peroxidation in muscle, liver, kidney and brain tissues of Cyprinus carpio were investigated. The 96-hours LC50 value for fenbutatin oxide was determined as 1.544 mg/L for C. carpio in this study. 0.15 mg/L (1/10 of LC50) and 0.30 mg/L (1/5 of LC50) sublethal concentrations were applied for 96 h (4 days) in this experiment. The CAT activities in tissues were increased relation to doses applied. The SOD activities were decreased by 0.30 mg/L. The tissue MDA levels were significantly increased in relation to dose applied.
Hepatoprotective Effect of Nigella Sativa L. Extract in Methyl Parathion Exposed Rats
(Parlar Scientific Publications (p S P), 2019) Berkoz, Mehmet; Kahraman, Tahir; Yildirim, Metin; Yigit, Muhammet Faruk; Allahverdiyev, Oruc
Methyl parathion (MP) is an organophosphorus pesticide that can induce hepatotoxicity in living organisms. Although many plant extracts have been utilized againts MP induced liver injury, it has not yet known whether Nigella sativa L. extract (NSE) has protective effects or not. We aim to investigate the hepatoprotective effect of NSE through the oxidative stress and anti-inflammatory pathways. A total of 28 rats were divided into 4 groups; control, MP, NSE and MP + NSE. We measured liver MDA level and antioxidant parameters, hepatic function enzyme, plasma cytokine levels, liver MPO activity, liver iNOS and COX-2 mRNA levels and MAPK/NF-kappa b protein levels. Our data showed that MP increased hepatic function tests, liver MDA level and MPO activity, plasma proinflammatory cytokines, liver iNOS and COX-2 rnRNA and MAPKs protein levels and decreased antioxidant parameters, antiinflammatory cytokine and liver NF-kappa b protein levels. NSE decreased hepatic function tests, liver MDA level and MPO activity, plasma proinflammatory cytokines, liver iNOS and COX-2 mRNA and MAPKs protein levels and increased antioxidant parameters, anti-inflammatory cytokine and liver NF-kappa b protein levels in the MP treated rats. These results suggested the antiinflammatory and antioxidative function of NSE in MP induced liver injury.
Influence of Sublethal Chlorpyrifos Exposure on Oxidative Stress and Acetylcholinesterase Activity in Common Carp (Cyprinus Carpio)
(Parlar Scientific Publications (p S P), 2019) Berkoz, Mehmet; Ozkan-Yilmaz, Ferbal; Ozluer-Hunt, Arzu; Gunduz, Suna Gul; Yildirim, Metin; Yalin, Serap
The commonly used pesticides in agriculture cause enzyme inactivation and DNA damage via reacting with macromolecules and may also initiate peroxidation of poly-unsaturated fatty acids (PUFA) by generating of the reactive oxygen species (ROS), as a consequence they can lead to the oxidative stress. The objective of this study was to determine the oxidative and neurotoxic potential of sublethal chlorpyrifos in the brain tissue of Cyprinus carpio (C. carpio) for a period of 96 and 240 hours, respectively. Therefore, the levels of malondialdehyde (MDA), glutathione (GSH), advanced oxidation protein products (AOPP), protein carbonyl, and 8-hydroxydeoxyguanosine (8-OHdG) levels and activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px) and acetylcholinesterase (AChE) were investigated in order to determine the neurotoxic effect in brain tissue of C. carpio exposed to chlorpyrifos. Administration of chlorpyrifos at 0.26 and 0.52 mg/L concentrations caused a significant increase in MDA levels at the 240th hour (p<0.05), the latter concentration increased the GSH level but decreased SOD, GSH-Px, and CAT activities at the 240th hour (p<0.05). Only 0.52 mg/L of chlorpyrifos administration increased protein carbonyl but not AOPP levels at the 240th hour (p<0.05). 0.26 and 0.52 mg/L of chlorpyrifos concentrations caused a significant increase 8-OHdG levels at the 96th and the 240th hours (p<0.05). Chlorpyrifos statistically significantly reduced the AChE levels in both periods and at all administered concentrations (p<0.05). We observed an enhanced oxidative stress and inhibited AChE activity in the brain tissue of C. carpio after exposure to chlorpyrifos. These findings show that sublethal concentration of chloropyrifos leads to significant toxicity in the brain tissues of C. carpio.
Myricetin Inhibits Angiotensin Converting Enzyme and Induces Nitric Oxide Production in Huvec Cell Line
(General Physiol and Biophysics, 2020) Berkoz, Mehmet; Yildirim, Metin; Yalin, Serap; Ilhan, Mert; Yunusoglu, Oruc
Nitric oxide is known as relaxing factor because it acts as a vasodilator, increases blood flow, and inhibits platelet aggregation and adhesion, on the other hand nitric oxide can modulate cellular and physiological processes to limit oxidative injury, limiting processes such as leukocyte adhesion. As the complete mechanism of myricetin and its cardiovascular benefits is not completely understood, the aim of this study was to investigate the antihypertensive activity of myricetin in human umbilical vein endothelial cell (HUVEC). Angiotensin converting enzyme (ACE) activity, nitric oxide production, reactive oxygen species (ROS) scavenger activity, cellular calcium concentration, and endothelial nitric oxide synthase (eNOS) activity and protein expression was investigated in HUVEC treated with different concentration of myricetin (1-60 mu M). Myricetin increased nitric oxide production in HUVEC through decreased ROS levels and increased nitric oxide production and eNOS activation. Activation of eNOS enzyme was achieved by an increase of cellular calcium concentration. At the same examined concentration of myricetin, the activity of ACE was significantly inhibited. These findings indicate that myricetin may be helpful for lowering blood pressure; this could be achieved through dietary intervention or by the production of new antihypertensive treatments from a natural product.
The Protective Effect of Alpha Lipoic Acid on Retinal Damage in Streptozotocin-Induced Diabetic Rats
(Parlar Scientific Publications (p S P), 2021) Berkoz, Mehmet; Yildirim, Metin; Bahar, Leyla; Sucu, Nehir; Ilhan, Mert; Yalin, Ali Erdinc
In this study, we examined the effects of alpha lipoic acid treatment on streptozotocin (STZ)-induced diabetic rats and investigated the mechanisms underlying the protective action of alpha lipoic acid in diabetic retinal damage which was induced by inflammation and oxidative stress and activation of MAPKs and NF-kappa B signaling pathways. A total of 28 rats were divided into 4 groups: control, diabetes mellitus (45 mg/kg STZ, alpha lipoic acid (100 mg/kg/day, gavage, for 21 days) and diabetes mellitus+ alpha lipoic acid. We measured malondialdehyde, and reduced glutathione levels and superoxide dismutase and catalase activities, vascular endothelial growth factor, nitric oxide and prostaglandin E2 levels, inducible nitric oxide synthase and cyclooxygenase-2 mRNA levels and MAPK/NF-kappa B protein levels in retina tissue. Alpha lipoic acid enhances antioxidant enzyme activities and inhibits NF-kappa B activation and IKB-alpha degradation, phosphorylation of p38. ERK, and JNK proteins, the expression of inducible nitric oxide and cyclooxygenase-2 mRNA, and production of nitric oxide, vascular endothelial growth factor, and prostaglandin E2. These results suggest that the antioxidative and anti-inflammatory activities of alpha lipoic acid may be important mechanisms involved in the protective effect of alpha lipoic acid on diabetic retinal damage in STZ-induced diabetic rats. Furthermore. alpha lipoic acid markedly reduced retinal injury associated with diabetes, as demonstrated by reduced histological damage of retinal cells.
Protective Effect of Myricetin, Apigenin, and Hesperidin Pretreatments on Cyclophosphamide-Induced Immunosuppression
(Taylor & Francis Ltd, 2021) Berkoz, Mehmet; Yalin, Serap; Ozkan-Yilmaz, Ferbal; Ozluer-Hunt, Arzu; Krosniak, Miroslaw; Francik, Renata; Yildirim, Metin
Aim: Major side effects of cyclophosphamide administration are immunosuppression and myelosuppression. The immunomodulatory effects of plant bioactive compounds on chemotherapy drug-induced immunosuppression may have significant effects in cancer treatment. For this reason, we investigated the immunomodulatory effect of myricetin, apigenin, and hesperidin in cyclophosphamide-induced immunosuppression in rats. Methods: In our study, a total of 64 rats were used, and divided into eight equal groups. These groups were: control, cyclophosphamide, cyclophosphamide+myricetin (100mg/kg), cyclophosphamide+myricetin (200mg/kg), cyclophosphamide+apigenin (100mg/kg), cyclophosphamide+apigenin (200mg/kg), cyclophosphamide+hesperidin (100mg/kg), and cyclophosphamide+hesperidin (200mg/kg). Myricetin, apigenin, and hesperidin pretreatments were performed for 14d, while cyclophosphamide application (200mg/kg) was performed only on the 4th day of the study. Levels of humoral antibody production, quantitative hemolysis, macrophage phagocytosis, splenic lymphocyte proliferation, and natural killer cell cytotoxicity were determined. In addition, we measured pro-inflammatory cytokines, and followed lipid peroxidation and antioxidant markers and examined the histology of bone marrow, liver and spleen in all groups. Results: During cyclophosphamide treatment, all three phytochemicals increased the levels of humoral antibody production, quantitative hemolysis, macrophage phagocytosis, splenic lymphocyte proliferation, antioxidant markers, and natural killer cell cytotoxicity. Moreover, the agents decreased the levels of pro-inflammatory cytokines and mediators, reduced lipid peroxidation markers, and reduced tissue damage in liver, spleen, and bone marrow. Conclusion: Our study demonstrated that myricetin, apigenin, and hesperidin can reduce the immunosuppressive effect of cyclophosphamide by enhancing both innate and adaptive immune responses, and these compounds may be useful immunomodulatory agents during cancer chemotherapy.
Rosa Canina L. Ethanolic Extract Induces the Anti-Proliferative and Apoptosis Potential in Mcf-7 and Mda-Mb Cell Lines
(Parlar Scientific Publications (p S P), 2019) Berkoz, Mehmet; Ozkan-Yilmaz, Ferbal; Ozluer-Hunt, Arzu; Yildirim, Metin; Allahverdiyev, Oruc; Aslan, Ali
Rosa canina L. (rose hip) fruits have been used for their diuretic, laxative, anti-gout, anti rheumatism properties in traditional medicine. Rose hip berries contain a variety of components such as flavonoid. The previous studies showed that flavonoid has anti-cancer properties. Tha aim of this study is to evaluate and screen the effect of apoptosis and the anticancer potential of rose hip ethanolic extract on human breast cancer cell lines; MCF-7 and MDA-MB-468. The anti-proliferative activity of rose hip extract was evaluated using MTT, flowcytometry by annexin V/PI double staining, and caspase-3 activity. The results of MTT showed that the EDso of both human breast cancer cell lines was 25 g/mL of rose hip extract, 48 hours after treatment. Flowcytometry by annexin V/PI showed that rose hip extract induced late apoptosis in MCF7 and early apoptosis in MDA-MB-468. In addition, the caspase-3 colorimetric method showed that caspase-3 increased in the MDA-MB-468 after treatment with rose hip extract. As a result, the ethanol of rose hip ethanolic extract induced apoptosis in both human breast carcinoma cell lines.
Thymoquinone Reduces the Inflammatory Response in Adipocytes
(Wiley-blackwell, 2016) Berkoz, Mehmet; Allahverdiyev, Oruc; Yildirim, Metin; Yalin, Serap