Browsing by Author "Yildiz, Damla"

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Effect of Acute and Chronic Fluoride Administration on Bone Histopathology, Bone Fluoride Accumulation, and Locomotor Activity in an Animal Model of Paleopathological Fluorosis
(int Soc Fluoride Research, 2020) Turkekul, Ramazan; Arihan, Seda Karaoz; Yildirim, Serkan; Arihan, Okan; Oto, Gokhan; Ekin, Suat; Yildiz, Damla
Fluorosis may be identified in skeletal materials from ancient civilizations by macroscopic signs in teeth and bone and hard tissue fluoride levels. In the present study, human teeth, femoral, and rib specimens from the Van Fortress excavation, in Turkey, were examined for the presence of fluorosis. In addition, an animal study in rats was conducted as a model of human fluorosis, by examining the effects of fluoride administration, in various doses and for different durations, on weight loss, locomotor activity, fluoride accumulation, and deformation in bone and teeth. Fifty-six adult male Wistar albino rats, weighing 150-200 g, were divided into 7 different groups of 8 rats. Four acute groups were treated with 0 (control), 5, 15, and 50 mg/L of fluoride in drinking water for 7 days and three chronic groups were treated with 5, 15, and 50 mg/L of fluoride for 90 days. The results of the analysis of the human samples from the Van Fortress excavation showed that none of the dental, femoral, or rib samples had a fluoride content that was significantly greater than that of the surrounding soil. The results of the rat study showed that no significant differences between the groups were found in body weight on days 1, 30, 60, and 90. The rotarod locomotor test showed a significant (p<0.05) dose- and time-dependent reduction in locomotor activity as a result of the fluoride administration in the 50 mg/L chronic fluoride group compared to the control, 5 mg/L acute fluoride, 15 mg/L acute fluoride, and 5 mg/L chronic fluoride groups. Significant fluoride accumulation was found in the femoral neck (cortical tissue), the femoral head (trabecular tissue), and in rib bone. Light microscopy showed a severe thinning of the epiphyseal growth plate and bone trabeculae in the femoral bone tissue. We concluded that femoral bone (cortical and trabecular parts) and ribs are good sites for assessing the effects of fluoride exposure in animal models of human fluorosis.
The Evaluation of Serum Total Sialic Acid and Lipid-Bound Sialic Acid Levels in Chronically Exposed Rats To 7,12-Dimethylbenz(a)anthracene and Fluoride
(Amer inst Physics, 2017) Oto, Gokhan; Ekin, Suat; Uyar, Hasan; Ozdemir, Hulya; Yildiz, Damla; Karakus, Yagmur
In this study, changes in serum total sialic acid (TSA) and lipid-bound sialic acid (LSA) levels were examined in chronically exposed rats to 7,12-dimethylbenz(a)anthracene (DMBA) and fluoride. This study demonstrated that the TSA, LSA levels increased more in DMBA-treated groups compared to the fluoride treated groups. The result obtained has shown that the harmful effect of DMBA which is also causing more cell membrane damage on human and animal health should be taken into consideration.
Evaluations of Antioxidant Enzyme Activities, Total Sialic Acid and Trace Element Levels in Coronary Artery Bypass Grafting Patients
(Soc Brasil Cirurgia Cardiovasc, 2021) Yildiz, Damla; Ekin, Suat; Sahinalp, Sahin
Introduction: In this study, patients before and after cardiac surgery with cardiopulmonary bypass (CPB) and control subjects were evaluated for erythrocyte glutathione peroxidase, catalase and superoxide dismutase enzyme activities, in addition to glutathione, malondialdehyde, serum total sialic acid, lipid-bound sialic acid, total antioxidant status, trace elements and mineral levels. The correlation of these variables with coronary artery disease (CAD) was also assessed. Methods: A total of 30 CAD patients and 30 control subjects were included in the study. CAD patients were divided into three groups: before surgery (BS), first day after surgery (1st day AS) and seventh day after surgery (7th day AS). Results: Malondialdehyde (MDA) and total sialic acid (TSA) levels were significantly higher in CAD (BS) than in the control group (P<0.05, P<0.05). In addition, GSH and TAS levels were significantly lower in the 1st day AS group than in the control group (P<0.001, P<0.01). Moreover, Co, Cu, Mg, Se, V and Zn levels were significantly lower in CAD (BS) group than in the control group (P<0.01, P<0.01, P<0.01, P<0.01, P<0.05, P<0.001). Conclusions: It was concluded that the levels of LDL-C, total cholesterol, triglycerides and CRP significantly associated with parameters, as well as Cu, Ca and SOD activity, should be measured together to monitor CAD. It is also considered that measuring TSA and MDA might be an appropriate choice for biomarkers of CAD.
Protective Effects of Saffron, Safranal and Crocin Administration on Vitamins (A, D, E, K) and Protein Carbonyl Levels Against Cci4-Induced Oxidative Damage in Rats
(Marmara Univ, Fac Medicine, 2024) Bakir, Ahmet; Yildiz, Damla; Ekin, Suat; Oto, Gokhan; Aras, Ibrahim; Bayram, Irfan
Objective: The possible effects of saffron and its active components on oxidative stress are known. Protein carbonyls (PCO), formed due to protein exposure to oxidizing agents, are a newly researched topic. In this study, it was aimed to determine, antioxidant fatsoluble vitamins (A, D, E, K) and PCO values after saffron, safranal and crocin administration with carbon tetrachloride (CCl4) in rats. Materials and Methods: Fifty-four Wistar albino male rats were randomly selected, and 9 groups of n=6 were formed. Vitamin levels in rat serum were determined by HPLC and PCO levels were determined by spectrophotometric method. Results: A significant difference (p<0.01) was found between the CCl4 and the saffron, safranal and crocin groups. A significant decrease was observed in retinol and cholecalciferol values between CCl4 and saffron group (p<0.05, p<0.001), and a significant decrease in cholecalciferol and phylloquinone levels between CCl4 and safranal groups (p<0.01, p<0.05). Moreover, a decrease in cholecalciferol level (p<0.05) was determined between the olive oil, saffron and CCl4+crocin groups. Conclusion: As a result, saffron and safranal have a protective effect against CCl4-induced oxidative damage to PCO, retinol, phylloquinone and cholecalciferol, and this effect may be due to the potent antioxidative effects of saffron and safranal.
Total Sialic Acid, Antioxidant Enzyme Activities, Trace Elements, and Vitamin Status Before and After Surgery in Women With Uterine Myoma and Endometrial Cancer
(Springer Heidelberg, 2023) Gok, Fazilet; Ekin, Suat; Karaman, Erbil; Erten, Remzi; Yildiz, Damla; Bakir, Ahmet
The objective of present study was to examine endometrial tissue Be, As, Cr, Mo, Sr, Ti, Tl, Cu, Co, Se, Zn, Mn, Fe, Cd, Pb, Mg, P, erythrocytes CAT, SOD, GSH-Px, GSH, MDA, serum retinol, cholecalciferol, phylloquinone, TSA, LSA, TOS, and TAS status and to evaluate the relationships between the variables. The study had 110 participants; of these, 50 were women with uterine myoma (UM), 10 were women with endometrial cancer (EC), and 50 were healthy female subjects. In the study, vitamin analyses by HPLC and element analyses were determined using ICP-OES method. It was observed that EC group was significantly lower than healthy group in terms of levels of cholecalciferol (p < 0.05), phylloquinone (p < 0.01), GSH (p < 0.05), Fe (p < 0.05), and had a significant rise in Mg/Fe (p < 0.01) and Zn/Fe (p < 0.05) in preoperative period. UM group had significantly lower retinol (p < 0.05), phylloquinone (p < 0.001), GSH-Px (p < 0.01), GSH (p < 0.01), Cr (p < 0.01), Cu (p < 0.05), Mg (p < 0.01), and Zn (p < 0.01) levels than control group in preoperative period and significantly higher levels of MDA (p < 0.01), TSA (p < 0.01), and LSA (p < 0.01) than control group. It was found that significant associations were observed between Cu-CA 15-3 (r = 0.558, p = 0.016), Mn-CA 15-3 (r = 0.511, p = 0.030), P-CA 15-3(r = - 0.502, p = 0.034) and with UM, also between GSH-CA-125 (r = - 0.825, p = 0.022) and with EC group. The results of correlation analysis observed that concentrations of Cu, Mn, P, and GSH together with CA 15-3 and CA-125 levels might be important for monitoring patients with UM and EC before surgery.