Browsing by Author "Yildiz, Fatos"

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Bisphenol S Leads To Cytotoxicity-Induced Antioxidant Responses and Oxidative Stress in Isolated Rainbow Trout (Oncorhyncus Mykiss) Hepatocytes
(Springer, 2021) Kaptaner, Burak; Yilmaz, Can; Aykut, Handan; Dogan, Emine; Fidan, Ceylan; Bostanci, Muserref; Yildiz, Fatos
Background Bisphenol S (BPS) is a chemical compound that is utilized in the plastic industry as an alternative to bisphenol A (BPA). The toxic effects of BPS in fish is less known and limited. Therefore, in the present study, the influence of BPS on rainbow trout (Oncorhyncus mykiss) hepatocytes in vitro was investigated. Methods and results For this purpose the fish hepatocytes were isolated, and then the cultured cells were treated with increasing concentrations of BPS (0, 15.63, 31.25, 62.50, 125, 250, and 500 mu M) for 24 h. The cytotoxic impact of BPS was determined in the culture media using lactate dehydrogenase assay and then, the antioxidant defence indicators were assayed. The results showed that concentration-dependent increases were observed in the percentage of cytotoxicity. The superoxide dismutase activity was reduced, while the catalase and glutathione peroxidase activity increased with all of the BPS concentrations. The glutathione S-transferase (GST) activity significantly increased after a BPS concentration of 31.25 mu M or higher, while GST Theta 1-1 activity was decreased by the same concentrations of BPS. The reduced glutathione content significantly decreased with a BPS concentration of 31.25 mu M or higher, and the malondialdehyde content increased after BPS concentrations of 125, 250, and 500 mu M. Conclusions The findings determined herein suggested that BPS causes cytotoxicity in fish hepatocytes and can lead to oxidative stress, resulting hepatotoxic in fish. Thus, the utilization of BPS instead of BPA as safe alternative in industry should be re-evaluated in the future for environmental health.
Histochemical Changes, Cellular Dynamics and Antioxidant Responses in the Corpuscles of Stannius of the Female Lake Van Fish (Alburnus Tarichi Guldenstadt, 1814) (Cyprinidae) During Migration From Highly Alkaline and Brackish Water To Freshwater
(Wiley, 2022) Kaptaner, Burak; Dogan, Abdulahad; Aykut, Handan; Yildiz, Fatos; Donmez, Fatih; Dogan, Emine
The Lake Van fish (Alburnus tarichi) is an anadromous species, which is endemic to Lake Van Basin of Turkey. In spring, the fish migrates from Lake Van, which has highly alkaline and brackish water, to freshwater to spawn. During the anadromous migration of the female Lake Van fish, histochemical changes in the cell types, cellular dynamics, apoptosis, cell proliferation and antioxidant responses in the corpuscles of Stannius (CS) which are tiny endocrine glands related with calcium homeostasis were examined. When compared to the lake, calcium concentrations of ambient water and plasma increased considerably in freshwater. Freshwater dramatically increased the number of predominant type-I cells in the gland while decreased the type-II cells. A reduction in the cytoplasmic periodic acid-Schiff content of type-I cells was observed in freshwater. No cytoplasmic staining was found in cytoplasm of type-II cells in both lake and freshwater. Apoptotic cells were detected in fish sampled from both the lake and freshwater and a nonsignificant increase was observed in freshwater. The number of proliferating cells increased significantly in the freshwater, as well as antioxidant defences such as catalase, glutathione peroxidase and glutathione s-transferase activities, which indicates an elevated oxidative status. The findings suggest that type-I cells are possible source of a hypocalcin hormone and type-II cells are considered to be a likely precursor of type-I cells. Apoptosis and cell proliferation are basic mechanisms of gland remodelling. In addition antioxidant mechanisms play a role during the acclimation of the gland in different environments.