Browsing by Author "Yildiz, Gulsum"

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Assessment of the Enzyme Inhibitory Activities of Salvia Dorystaechas and Its Phytochemical Analysis by Rp-Hplc
(Springer, 2025) Ozupek, Burcin; Pekacar, Sultan; Saltan, Nagehan; Yildiz, Gulsum; Gulcan, Zeynep; Orhan, Didem Deliorman; Kose, Yavuz Bulent
Salvia dorystaechas is an endemic species found around Antalya in Anatolia, and in this study, the antidiabetic, antiobesity, antihyperlipidemic, and antimelanogenic potentials of the extracts (methanol, ethyl acetate, and chloroform) obtained from the root and aerial parts of the plant were evaluated using in vitro enzyme models (alpha-glucosidase, alpha-amylase, pancreatic lipase, cholesterol esterase, and tyrosinase). Root ethylacetate (IC50 6.53 +/- 0.19 mu g/ml) and aerial part chloroform extracts (IC50 24.14 +/- 1.76 mu g/ml) inhibited the pancreatic cholesterol esterase enzyme, as much as, or even more potently than simvastatin (IC50 20.48 +/- 2.70 mu g/ml). All extracts showed weak or no inhibitory activity against other enzymes. RP-HPLC analysis results showed that S. dorystaechas root ethyl acetate extract, which has the strongest pancreatic cholesterol esterase inhibitory activity, can be standardized with rosmarinic acid (3.164 +/- 0.001 mg/g plant) and caffeic acid (1.118 +/- 0.000 mg/g plant). As a result, it can be predicted that this plant, which is also commonly consumed as a tea, can be evaluated as a medicinal tea or medicinal herbal product with further studies due to its strong cholesterol-lowering effect.
Chemical Profile, in Vitro Pharmacological Activity and Satureja Cuneifolia Ten. Evaluation of Essential Oil Based on Distillation Time
(Taylor & Francis Ltd, 2024) Yildiz, Gulsum; Ilgun, Selen; Karatoprak, Gokce Seker; Kose, Yavuz Bulent; Goger, Fatih; Temel, Halide Edip; Demirci, Betul
The medicinal plant Satureja cuneifolia Ten. was widely utilized as spice, tea and traditional medicine. The objective of the current study was to examine the chemical composition and in vitro biological activities (LOX, MMP-1, and MMP-12 enzyme inhibition activity and cytotoxicity on A549 cell line) of Satureja cuneifolia extracts and essential oils. The essential oils of the flowering aerial parts were hydro-distilled at four different distillation times (5, 30, 60, and 180 min) using the Clevenger apparatus. The total essential oil and four fragments were compared in terms of the major component, yield, and distillation time. Volatile compounds of the infusion were extracted by using HS-SPME. Ethanolic extract had the strongest inhibition activity on the LOX enzyme (84.50%), while the essential oils exhibited more cytotoxic activity on the A549 cell line than the extracts. The oils and the infusion were analyzed using GC-MS and the primary chemicals identified by LC-MS/MS.
Origanum Minutiflorum O. Schwarz Et P. H. Davis Essential Oil: Enzyme Inhibitory Activities and Chemical Composition
(Marmara Univ, 2023) Yildiz, Gulsum; Demirci, Betuel; Temel, Halide Edip; Kirimer, Nese
The essential oil (EO) of Origanum minutiflorum O. Schwarz et P. H. Davis was obtained by the hydrodistillation method. The analysis of the EO was conducted using Gas Chromatography (GC) and Gas Chromatography/Mass Spectrometry (GC/MS). The yield of the EO was determined to be 3.9%. Fifty-six compounds were identified, constituting 97.8% of the EO. The EO was rich in carvacrol at a rate of 83.3%, and the other major compounds were p-cymene (3.0%), beta-caryophyllene (1.3%), trans-sabinene hydrate (1.1%), gamma-terpinene (1.1%), borneol (1.1%), and terpinene-4-ol (1.0%). While the EO (100 mu g/mL) demonstrated the highest inhibition on cyclooxygenase-1 (COX-1) with 55.26%, the inhibitory activities on the other enzymes were as follows: cyclooxygenase-2 (COX-2); 33.10%, and matrix metalloproteinase-9 (MMP-9); 12.87%. The EO had no inhibition on lipoxygenase (LOX). In this research, inhibitory activity of the EO of O. minutiflorum on COXs and MMP-9 enzymes was reported for the first time.
Origanum Minutiflorum: Phytochemical Profile and Inhibitory Effects on Key Enzymes Associated With Inflammation
(Taylor & Francis Ltd, 2023) Yildiz, Gulsum; Temel, Halide E.; Agalar, Hale G.; Kirimer, Nese
Origanum minutiflorum, an endemic species in Turkiye, is used for both food and medicinal purposes. 70% ethanol extract of aerial parts of O. minutiflorum was subjected to liquid-liquid extraction using; n-hexane, dichloromethane, ethyl acetate and n-butanol. The inhibitory activity of these fractions on lipoxygenase (LOX), cyclooxygenase (COX) and matrix metalloproteinase-9 (MMP-9) was investigated at 100 mu g/mL concentration. The LOX enzyme was most effectively inhibited by the dichloromethane fraction (82.33%), whereas the COX-1, COX-2 and MMP-9 enzymes were most effectively inhibited by the ethyl acetate fraction (78.50%, 83.96% and 45.11%, respectively). The ethyl acetate fraction was selected for activity-guided fractionation based on the COX enzymes' inhibitory activities by column chromatography. Only the ethyl acetate sub-fraction-1 showed inhibition on the COX-1 with 65.78%. The COX-2 inhibitory activities of the sub-fractions ranged from 59.96% to 97.62%. The main components were determined by HPLC-MS/MS as jaceidine in the dichloromethane fraction and rosmarinic acid in the ethyl acetate fraction. As far as we know, this study is the first to correlate the anti-inflammatory activity of O. minutiflorum extracts with LOX, COX and MMP-9 enzyme pathways and their secondary metabolites. These data showed that O. minutiflorum may contribute to other studies on natural origin anti-inflammatory agents.
Phytochemical Composition and Biological Activities of Arctium Minus (Hill) Bernh.: a Potential Candidate as Antioxidant, Enzyme Inhibitor, and Cytotoxic Agent
(Mdpi, 2022) Ilgun, Selen; Karatoprak, Gokce Seker; Polat, Derya Cicek; Safak, Esra Kongul; Yildiz, Gulsum; Akkol, Esra Kupeli; Sobarzo-Sanchez, Eduardo
Arctium minus (Hill) Bernh. (Asteraceae), which has a wide distribution area in Turkey, is a medicinally important plant. Eighty percent methanol extracts of the leaf, flower head, and root parts of A. minus were prepared and their sub-fractions were obtained. Spectrophotometric and chromatographic (high-performance liquid chromatography) techniques were used to assess the phytochemical composition. The extracts were evaluated for antioxidant activity by diphenyl-2-picrylhydrazil radical (DPPH?), 2,2 '-Azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS(?+)) radical scavenging, and beta-carotene linoleic acid bleaching assays. Furthermore, the extracts were subjected to alpha-amylase, alpha-glucosidase, lipoxygenase, and tyrosinase enzyme inhibition tests. The cytotoxic effects of extracts were investigated on MCF-7 and MDA-MB-231 breast cancer cell lines. The richest extract in terms of phenolic compounds was identified as the ethyl acetate sub-fraction of the root extract (364.37 +/- 7.18 mg(GAE)/g(extact)). Furthermore, chlorogenic acid (8.855 +/- 0.175%) and rutin (8.359 +/- 0.125%) were identified as the primary components in the leaves' ethyl acetate sub-fraction. According to all methods, it was observed that the extracts with the highest antioxidant activity were the flower and leaf ethyl acetate fractions. Additionally, ABTS radical scavenging activity of roots' ethyl acetate sub-fraction (2.51 +/- 0.09 mmol/L Trolox) was observed to be as effective as that of flower and leaf ethyl acetate fractions at 0.5 mg/mL. In the beta-carotene linoleic acid bleaching assay, leaves' methanol extract showed the highest antioxidant capacity (1422.47 +/- 76.85) at 30 min. The enzyme activity data showed that alpha-glucosidase enzyme inhibition of leaf dichloromethane extract was moderately high, with an 87.12 +/- 8.06% inhibition value. Lipoxygenase enzyme inhibition was weakly detected in all sub-fractions. Leaf methanol extract, leaf butanol, and root ethyl acetate sub-fractions showed 99% tyrosinase enzyme inhibition. Finally, it was discovered that dichloromethane extracts of leaves, roots, and flowers had high cytotoxic effects on the MDA-MB-231 cell line, with IC50 values of 21.39 +/- 2.43, 13.41 +/- 2.37, and 10.80 +/- 1.26 mu g/mL, respectively. The evaluation of the plant extracts in terms of several bioactivity tests revealed extremely positive outcomes. The data of this study, in which all parts of the plant were investigated in detail for the first time, offer promising results for future research.