Browsing by Author "Yildiz, Ismail"

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Frontonia Anatolica N. Sp., a New Peniculid Ciliate (Protista, Ciliophora) From Lake Van, Turkey
(Tubitak Scientific & Technological Research Council Turkey, 2013) Yildiz, Ismail; Senler, Naciye Gulkiz
The morphology, ciliature, and silverline system of a new ciliate, Frontonia anatolica n. sp., isolated from the bottom sediment of the eastern shore of Lake Van, a large alkaline lake in Eastern Anatolia Turkey, were investigated using live and silver impregnation methods. Frontonia anatolica n. sp. is characterised by an elliptical body shape; by an in-vivo body size of 101-134 x 47-67 mu m; by dorsoventral flattening of about 2:3 to 1:2; by 2 contractile vacuoles located in the anterior and posterior body parts, each with 6-7 collecting canals and 1 excretory pore; by about 93 somatic kineties; by 3 vestibular and 3-5 postoral kinetics; and by peniculi 1 and 2 each having 4 ciliary rows, and peniculus 3 having 2 ciliary rows.
Morphology and Infraciliature of Some Haptorid Ciliates (Protista, Ciliophora, Haptoria) in Alkaline Soil Samples of Van (Turkey), With Notes on the Ontogenesis of Enchelyodon Nodosus Berger, Foissner & Adam, 1984
(Tubitak Scientific & Technological Research Council Turkey, 2009) Senler, Naciye Guelkiz; Yildiz, Ismail
The morphology and infraciliature of 4 haptorid ciliates obtained from alkaline soil habitats in Van, Turkey, were investigated: Pseudoholophrya terricola Berger, Foissner & Adam, 1984, Paraenchelys wenzeli Foissner, 1984, Armatoenchelys geleii (Foissner, 1981), and Enchelyodon nodosus Berger, Foissner & Adam. 1984. The descriptions were based on live observations and silver impregnated specimens and morphometry. The main morphological characteristics of these species are similar to those described in the literature. However, the dorsal brush row 3 is longer than dorsal brush rows 1 and 2 in A. gelen, and E. nodosus has a long monolkinetidal tail not reported previously. This is the first record of E. nodosus after the original description and also the first study on its ontogenesis. Division of E. nodosus is homothetogenic and telolkinetal; it commences with a proliferation of kinetosomes in those kineties bearing the dorsal brush. All species are new for the ciliate fauna of Turkey.
Morphology and Molecular Phylogeny of Blepharisma Hyalinum Perty, 1849 (Ciliophora, Heterotrichida) Isolated From Soil
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2025) Senler, Naciye Gulkiz; Yildiz, Ismail; Ural, Hilal; Bircan, Rifat
In this study, two successive populations of Blepharisma were observed in cultures prepared from soil samples collected from Tekirda & gbreve; (T & uuml;rkiye). The cells and cysts belonging to these populations were examined morphologically and morphometrically, and their phylogenetic positions were determined based on SSU rDNA gene sequences. Both populations were morphometrically very similar to each other, but population 1, the initial population observed in soil cultures, had a pyriform body that was inflexible and rigid while population 2, observed subsequent to the disappearance of population 1, exhibited a flexible, fragile, lanceolate body. In addition, the cell width/length ratios of both populations were found to be statistically different from each other. However, since the SSU rDNA nucleotide sequences of both populations are very similar to each other and to other B. hyalinum populations described previously, it is concluded that the observed Blepharisma populations represent different morphotypes of B. hyalinum. It has been hypothesised that the observed morphological differences may result from diet, environmental factors, and variations in the life stages of the cultured cells. Comparison of SSU rDNA nucleotide sequences of Blepharisma populations and phylogenetic analyses showed that the nucleotide sequences of some different species are identical or very similar. To clarify this chaotic situation, extensive molecular data based on detailed morphological studies on Blepharisma populations are necessary.
Morphology and Molecular Phylogeny of Four Frontonia Species From Turkey (Protista, Ciliophora)
(Magnolia Press, 2019) Kizildag, Sibel; Yildiz, Ismail
In this study, Frontonia leucas, Frontonia acuminata, Frontonia angusta, and Frontonia anatolica species isolated from aquatic environments of Van in Turkey were investigated in detail using morphological, morphometrical, and molecular methods. Although there were minor differences, the Frontonia populations were morphologically similar to the other previously reported populations of the 4 species. Frontonia leucas differed from the other populations by the following combination of characters: about 200 somatic and only 3 vestibular kineties, and a single micronucleus. The Turkish population of Frontonia acuminata had just 4 vestibular kineties and the large micronucleus was always located to the anterior of the carrot-shaped macronucleus. The ratio of the oral apparey size to the cell size of Frontonia angusta in this study was about 18%, with 3 vestibular kineties, and 1 excretory pore. Phylogenetic trees based on small-subunit rRNA gene sequences were constructed using Bayesian inference and maximum-likelihood. Frontonia anatolica was more closely related to Apofrontonia dohrni and Paramecium spp. than to its congeners, while F. acuminata, like F. terricola, was also more closely related to the family Stokesiidae. The results indicated that Frontonia is a non-monophyletic genus consisting of 3 groups. We presented the systematic relationships of the genera and families of Peniculida with new data of genus Frontonia herein.
Morphology and Phylogeny of a New Soil Ciliate, Colpodidium Zelihayildizae N. Sp. (Ciliophora, Nassophorea, Colpodidiidae), From Van, Turkey
(Tubitak Scientific & Technological Research Council Turkey, 2021) Yildiz, Ismail
A new ciliate species Colpodidium zelihayildizae n. sp., which was discovered in a dry irrigation channel in Van (Turkey), was investigated morphologically using standard live and silver impregnation methods, and its SSU rRNA gene phylogeny was provided. This new species was characterized as follows: body size about 40-60 x 15-20 mu m in vivo, elongated ellipsoidal with flat or slightly concave ventral surface; the buccal cavity slightly below equatorial plane; macronucleus spherical or slightly elliptical, usually located in the last third of the cell; cortex distinctly furrowed in the anterior half; 19-22 somatic kineties, 4 postoral kineties; paroral membrane has 20 dikinetids; 3 nassulid organelles (NOs), with NO1 very close to the left edge of the paroral membrane, which consists of 1 dikinetid pair. Phylogenetic analyses conducted on the SSU rDNA sequences determined that Colpodidium zelihayildizae was clustered with Colpodidium caudatum and formed a clade with Apocolpodidium etoschense and Colpodididae sp.
Morphology and Phylogeny of Apertospathula Oktemae N. Sp. (Ciliophora, Haptoria, Spathidiida) From Lake Van, Turkey
(Elsevier Gmbh, 2018) Yildiz, Ismail
A new spathidiid ciliate, Apertospathula oktemae n. sp., was isolated from bottom sediments on the eastern shore of Lake Van, Turkey. The living cells are clavate and 45-80 x 17-30 mu m in size. This species is characterized by a lasso-shaped circumoral kinety composed of more than 100 dikinetids, 16 meridionally arranged somatic ciliary rows, a three-rowed dorsal brush with 1.5-2.0 mu m long bristles, an oblong and curved macronucleus with an ellipsoidal or globular micronucleus, numerous refractive granules in the anterior portion of the cell, and a single posterior contractile vacuole. This new species lives in alkaline brackish water. Apertospathula oktemae differs from congeners by their body shape, number of oral dikinetids, presence of refractive granules, and their habitat. This is the first study to investigate the 18S rRNA gene sequence of a member of the genus Apetrospathula. Phylogenetic trees show that Apertospathula oktemae is most closely related to Arcuospathidium sp. There is a discrepancy between the morphological classification system of the spathidiid ciliates and their molecular phylogeny. To overcome this problem, more molecular data, obtained from more taxa from various geographical regions of the world, are needed. (C) 2018 Published by Elsevier GmbH.
A New Approach To Dna Isolation From Dileptid Ciliates (Ciliophora: Litostomatea: Rhynchostomatia)
(inst Zoology, Bas, 2020) Ural, Hilal; Bircan, Rifat; Aral, Cenk; Yildiz, Ismail; Senler, Naciye Gulkiz
Molecular diagnostic methods have been used to supplement morphological methods in taxonomy. In this study, we used molecular methods to diagnose populations of dileptid ciliates, which commonly occur in terrestrial and semi-terrestrial habitats. Molecular investigations based on the comparison of DNA sequences have been carried out with a limited number of ciliate species due to insufficient genomic DNA for PCR because of their small population size and difficulties to maintain monocultures. The present study shows that there is a loss of DNA with conventional methods and proposes a novel approach: cells (stored at -20 degrees C or fresh) are directly subjected to PCR, without being processed by any chemical treatment. The small subunit ribosomal DNA (SSU rDNA) (18S rRNA gene) of dileptid ciliates was successfully amplified by direct PCR, without DNA extraction, for single-cell and multi-cell samples. This method has been found to be simpler and especially useful for species that are rare and have small population sizes.