Browsing by Author "Yilmaz, A"

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Immunohistochemical Localization of Cytokeratin, Vimentin and Alpa-Smooth Muscle Actin Micro and Intermediate Filaments in Canine Mammary Tumors
(Scientific Technical Research Council Turkey, 2000) Milli, UH; Haziroglu, R; Yilmaz, A; Aydin, Y; Gülbahar, MY
Forty-six formalin-fixed paraffin-embedded canine mammary tumors, 15 benign and 31 malignant, were studied immunohistochemically. Mouse monoclonal anti-alpha-cytokeratin 18, mouse monoclonal anti-alpha-cytokeratin (pan cytokeratin), mouse monoclonal anti-alpha-smooth muscle actin and mouse monoclonal anti-vimentin antibodies were used. The ductal and acinar epithelium stained positively for cytokeratin 18 and pan cytokeratin, although there was no immunoreactivity in some tumors. Metaplastic squamous cells in two solid carcinomas were positive for both cytokeratin 18 and pan cytokeratin. It was a notable finding that there were unreactive epithelial cells in some tumors for broad spectrum cytokeratin antibody (pan cytokeratin). Myofibroblasts in benign and malignant tumors were positive for both vimentin and alpha-smooth muscle actin antibodies. Stromal mesenchymal tissues, including metaplastic cartilage and bone, stained strongly positive for vimentin antibody. The majority of cells in two spindle cell carcinomas were immunoreactive with alpha-smooth muscle actin, being indicative of the myoepithelial origin of these tumors. Myoepithelial proliferation was prominent in ductal papilloma (1/1), benign mixed tumor (1/5), tubular adenocarcinomas (3/6), papillary adenocarcinomas (3/4), papillary cystic adenocarcinomas (2/3), solid carcinomas (4/8) and malignant mixed tumors (2/4). These proliferated cells stained positively for alpha-smooth muscle actin antibody except for those of a tubular adenocarcinoma, papillary adenocarcinoma and solid carcinoma. The absence of immunoreactivity with alpha-smooth muscle actin in chondromucinous stroma, cartilage and bone indicated that cartilage and bone formation in canine mammary tumors results from stromal metaplasia. The results of this study provide further evidence for the value and usefulness of immunohistochemical methods using monoclonal antibodies in formalin-fixed paraffin-embedded canine mammary tumors.
Phytic Acid and Phosphorus Concentrations in Seeds of Wheat Cultivars Grown With and Without Zinc Fertilization
(Taylor & Francis inc, 2002) Erdal, I; Yilmaz, A; Taban, S; Eker, S; Torun, B; Cakmak, I
Seeds of twenty wheat cultivars grown with (+Zn = 23 kg Zn ha(-1)) and without zinc (Zn) fertilization in a Zn-deficient calcareous soil in Central Anatolia were analyzed for the levels of Zn, phosphorus (P), phytic acid, and phytase activity. Additionally, seeds of four wheat cultivars grown on 55 different locations in Turkey were also analyzed for Zn, P, and phytic acid. In the field experiment with 20 wheat cultivars, seed Zn concentrations showed a range between 7 to 11 mg kg(-1) under Zn-deficient and 14 to 23 mg kg(-1) under Zn-added conditions. Zinc fertilization reduced seed concentrations of P and phytic acid of all cultivars. On average, the reductions caused by Zn fertilization were from 3.9 to 3.5 mg g(-1) for P and from 10.7 to 9.1 mg g(-1) for phytic acid. Irrespective of Zn fertilization, seed phytic acid concentrations showed a large genotypic variation, i.e., from 7 to 12 mg g I with Zn fertilization and 8 to 13 mg g I at nil Zn treatment. As a result of decreases in phytic acid and increases in Zn concentrations by Zn fertilization, phytic acid to Zn molar ratios in seeds of cultivars markedly decreased. On average for ail cultivars, phytic acid to Zn molar ratios decreased from 126 to 56 with Zn fertilization. Seed phytase activity of cultivars was not consistently influenced by varied Zn supply. However, on average for 20 cultivars, Zn fertilization tended to decrease phytase activity. In seeds of four wheat cultivars collected from 55 locations, the concentrations of Zn, P, and phytic acid ranged from 8 to 34 mg kg(-1), 2.1 to 4.9 mg g(-1), and 5.8 to 14.3 mg kg(-1), respectively. Results obtained in the present study indicate that seed Zn concentrations of wheat cultivars grown in different locations of Turkey, especially under Zn-deficient conditions, are very low. Considering very high phytic acid: Zn molar ratios it can be suggested that bioavailability of Zn would be very low for humans.
Some Factors Affecting Milk Production and Post Partum Body Weight of Fat-Tailed Norduz Ewes in Turkey
(Garuda Scientific Publications, 2005) Bingöl, M; Aygün, T; Gökdal, Ö; Yilmaz, A
This study was conducted to determine some traits of milk production and postpartum body weights of Norduz ewes in Van-Norduz provinces. Overall means for lactation period, lactation milk production and post partum body weight were 174 days, 178 liters and 58 kg, respectively. Only post partum body weight was affected (P < 0.05) by age of ewe or parturition type. The Norduz ewe has been recommended for milk production.
Some Testis Characteristics in Norduz Male Kids
(Scientific Technical Research Council Turkey, 2003) Aygün, T; Yilmaz, A
This study was carried out to investigate the growth of some testis characteristics of Norduz male kids reared at the Agricultural Farm of Yuzuncu Yil University. The data were obtained from 22 Norduz male kids weaned at 3 months of age. The male kids were maintained in farm conditions. In male kids, least square means of testis diameter, testis length, scrotum circumference and scrotum length evaluated as testis measurements were 3.13 +/- 0.08, 5.95 +/- 0.14, 16.52 +/- 0.39 and 10.60 +/- 0.33 cm at 90 days, respectively, and 4.75 +/- 0.08, 9.29 +/- 0.14, 19.61 +/- 0.40 and 12.38 +/- 0.34 cm at 258 days, respectively. The effects of live weight of kid, dam age, type of birth and measuring periods on testis diameter, testis length, scrotum circumference and scrotum length were very significant (P < 0.01). The results of this study showed that the growth of testis characteristics in Norduz male kids was at optimal level.