Browsing by Author "Yilmaz, Hilal"

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Chromosome Analysis of Some Phaseolus Vulgaris L. Genotypes in Turkey
(Bangladesh Botanical Soc, 2022) Kulaz, Haluk; Najafi, Solmaz; Tuncturk, Murat; Tuncturk, Ruveyde; Yilmaz, Hilal
Karyotypes parameters in four selected Turkish bean genotypes (BT, HK, VN, and EL) were studied. Genotypes (4 levels) and chromosomes (11 levels) were compared in factorial experiment based on completely randomized design with five replications. Genotypes showed significant differences in long arm, short arm and total chromosome length and divided into three separate groups. Genotypes BL and HK were placed together in a separate group, probably showing the weaker kinship with the other genotypes. Also, the significant differences in all recorded parameters were observed among chromosomes of each genotype. All genotypes were diploid 2n = 22 with no satellite and differed significantly in karyotypic parameters. The most and the least chromatin length (34.24 mu m and 15.65 mu m) were observed in BT and EL genotypes, respectively. Also, all genotypes showed high chromosomal symmetry and categorized in Stebbins's Class 1A. The karyotype of genotype BT was found to be more symmetrical than others.
Effects of Humic Acid and Edta on Phytoremediation, Growth and Antioxidant Activity in Rapeseed (Brassica Napus L.) Grown Under Heavy Metal Stress
(Hard, 2022) Canal, Sibel Boysan; Bozkurt, Mehmet Ali; Yilmaz, Hilal
Rapeseed has been cultivated to produce non-edible and edible oil for thousands of years. It is known as the second-largest oilseed plant in the world with 24.6 million tons of oil production in 2021. The interventions that can be carried out during the cultivation of a plant with such a high production value are quite significant. Growth, enzymatic activities, and phytoremediation of rapeseed grown under heavy metal stress supported by humic acid (HA) and ethylenediaminetetraacetic acid (EDTA) applications were investigated for the first time in this study. Three doses of EDTA (EDTA1:5 mmol/kg, EDTA2:10 mmol/kg, EDTA3:15 mmol/kg) and three doses of HA (HA1:500 mg/kg, HA2:1000 mg/kg, HA3:2000 mg/kg) were applied in heavy metal treated pots. According to experiment results, HA1 and HA2 applications increased plant dry and fresh weights, root dry and fresh weights. However, EDTA applications caused a decrease in shoot length, a number of leaves, shoot fresh and dry weights, root fresh and dry weights. Bioconcentration factor (BCF) values for Zn, Cr and Cd we found higher than in both shoots and roots of rapeseed. For all levels of EDTA, the values of BCF (shoot) and BCF (root), transfer factor (TF) and translocation factor (TLF) increased compared to HA applications. On the other hand, in comparison to heavy metal polluted soils alone (PS), all levels of HA resulted in significantly reduced APX and CAT enzyme activity, and hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents. We concluded that humic substances exert a significant influence on plant growth and reduce heavy metal toxicity in polluted soils. At the same time, HA could be more effective than EDTA in terms of phytoremediation of Cr. HA can increase phytoremediation in polluted soils as it improves plant growth and oxidative stress due to its organic nature. The results provide remarkable information for rapeseed cultivation in polluted regions.
The Effects of Plant Growth Promoting Rhizobacteria on Antioxidant Activity in Chickpea (Cicer Arietinum L.) Under Salt Stress
(Agricultural Research Communication Centre, 2019) Yilmaz, Hilal; Kulaz, Haluk
In chickpea soil salinity is one of the most important factors affecting yield, nodulation and physiological events. Salinity affects the growth of salt sensitive varieties. The inoculation of plant growth promoting rhizobacteria (PGPR) allows to reduce the harmful effects of salinity. To prevent adverse effects of chickpea salinity, the effects of four bacteria (Rhizobium ciceri, A-08, EB-80 and Isolate-30) in root rhizosphere under controlled environmental growth conditions were studied. This study has shown that PGPRs play an important role in growth regulators for the positive development of plants under salt stress. It has been observed that these isolates, common in roots, are tolerant to salinity antioxidant activity and an increase in proline, MDA, APX, SOD and CAT concentrations were found under saline conditions when unvaccinated plants were compared with grafted plants. The results also suggested that inoculated PGPR strains can reduce salinity stress by increasing salt tolerance.
Humic Acid Ameliorates Phytoremediation, Plant Growth and Antioxidative Enzymes in Forage Turnip (Brassica Rapa L.)
(Czech Academy Agricultural Sciences, 2023) Canal, Sibel Boysan; Bozkurt, Mehmet Ali; Yilmaz, Hilal
In this study, the effects of ethylenediaminetetraacetic acid (EDTA) and humic acid (HA) chelate applied to soils contaminated with heavy metals on the development, antioxidant defence system, and phytoremediation of forage turnip (Brassica rapa L.) were investigated for the first. Three doses of EDTA (E1: 5 mmol/kg, E-2: 10 mmol/kg, E-3: 15 mmol/kg) and three doses of HA (HA1: 500 mg/kg, HA(2): 1 000 mg/kg, HA(3): 2 000 mg/kg) were applied to soils contaminated with heavy metals (Cd, Pb, Zn, and Cr) in the pot. According to experiment results, HA application as chelate to the polluted soil caused a significant increase in the growth of forage turnip. Phytoremediation values of the plant for Cd heavy metal were found to be BCFs(hoot), BCFroot > 1, and translocation factor > 1. This result proved that forage turnip has Cd accumulating properties. Also, HA application caused a decrease in H2O2 (46%) and malondialdehyde (6%) levels and antioxidative enzyme activity in polluted soil. It has been concluded that humic acid improves the oxidative stress conditions in the plant and is more effective in the development and growth of the plant than EDTA, so that it can be used effectively in phytoremediation studies.