Browsing by Author "Yoruk, Ibrahim H."

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Effects of Extract of Green Tea and Ginseng on Pancreatic Beta Cells and Levels of Serum Glucose, Insulin, Cholesterol and Triglycerides in Rats With Experimentally Streptozotocin-Induced Diabetes: a Histochemical and Immunohistochemical Study
(Medwell online, 2010) Karaca, Turan; Yoruk, Mecit; Yoruk, Ibrahim H.; Uslu, Sema
This study investigated the effects of oral administration of extract of green tea (Camellia sinensis) and ginseng (American ginseng-Panax quinquefolium L.), given alone or together, on pancreatic beta-cells, blood glucose, insulin, cholesterol and triglyceride levels in rats with experimental diabetes induced by a single injection of Streptozotocin (STZ) (60 mg kg(-1), i.p.). Fifty adult Wistar Albino rats were used, 10 in each of these five treatment groups: Group A: healthy controls, Group B: STZ-induced diabetes (untreated), Group C: STZ-induced diabetes plus green tea extract (100 mg/kg/daily), Group D: STZ-induced diabetes plus ginseng root (400 mg/kg/daily) and Group E: STZ-induced diabetes plus ginseng root + green tea extracts as before. At the end of the 6 weeks experiment, blood samples were analysed for blood glucose, insulin, cholesterol and triglyceride levels and samples of pancreatic tissue were examined histochemically and immunohistochemically for endocrine islets and beta-cells. Overall, body weight decreased in groups B and C, serum insulin concentrations decreased slightly in groups C-E and total triglyceride levels of blood decreased significantly (p<0.05) in groups B and C compared with control, D and E groups. Histopathological examination showed that degenerative changes in pancreatic beta-cells in STZ-treated rats were minimised to near normal morphology by administration of ginseng (Group D) and ginseng+green tea (Group E) and there was increased intensity of immunohistochemical staining for insulin in these groups. Degeneration of islets of Langerhans beta-cells and weak insulin staining was observed for green tea alone (Group C). These findings demonstrate that ginseng or combined ginseng + green tea decreases blood glucose levels in diabetic rats and increases preservation of P-cells, perhaps by lowering oxidative stress.
Selected Maternal, Fetal and Placental Trace Element and Heavy Metal and Maternal Vitamin Levels in Preterm Deliveries With or Without Preterm Premature Rupture of Membranes
(Wiley, 2018) Kucukaydin, Zehra; Kurdoglu, Mertihan; Kurdoglu, Zehra; Demir, Halit; Yoruk, Ibrahim H.
Aim: To compare maternal, fetal and placental trace element (magnesium, zinc and copper) and heavy metal (cadmium and lead) and maternal vitamin (retinol, alpha [alpha]-tocopherol, vitamin D-3, 25-hydroxyvitamin D-3 and 1,25-dihydroxyvitamin D-3) levels in preterm deliveries with and without preterm premature rupture of membranes (PPROM). Methods: Sixty-eight patients giving birth preterm were grouped into preterm deliveries with PPROM (n = 35) and without PPROM (n = 33). Following delivery, maternal and umbilical cord blood sera and placental tissue samples were obtained. While magnesium, zinc, copper, cadmium and lead levels were measured in all samples, the levels of retinol, -tocopherol, vitamin D-3, 25-hydroxyvitamin D-3 and 1,25-dihydroxyvitamin D-3 were measured only in maternal serum. Results: While magnesium level in maternal serum and zinc levels in both maternal and umbilical cord sera were lower, placental magnesium level was higher in preterm deliveries with PPROM (P < 0.01). Umbilical cord serum magnesium, placental tissue zinc and maternal and umbilical cord sera and placental tissue copper, cadmium, and lead levels did not differ between the groups (P > 0.05). In preterm deliveries with PPROM, 25-hydroxyvitamin D-3 and retinol levels were higher, while vitamin D-3 and 1,25-dihydroxyvitamin D-3 levels were lower in maternal serum (P < 0.05). Maternal serum -tocopherol levels were similar between the groups. Conclusion: Compared to spontaneous preterm births, PPROM is associated with low maternal serum together with high placental tissue magnesium and low maternal and umbilical cord sera zinc levels. Higher retinol and 25-hydroxyvitamin D-3 and lower vitamin D-3 and 1,25-dihydroxyvitamin D-3 maternal serum levels are also evident in these patients.
Serum Selenium, Vitamin E, and Sialic Acids Concentrations in Lambs With White Muscle Disease
(Humana Press inc, 2008) Deger, Yeter; Mert, Handan; Mert, Nihat; Yur, Fatmaguel; Kozat, Sueleyman; Yoruk, Ibrahim H.; Sel, Tevhide
The aim of this study was to determine the serum concentrations of selenium, vitamin E, and total- and lipid-bound sialic acid (LBSA) in lambs with white muscle disease (WMD) before and after treatment with a commercial preparation containing selenite and vitamin E. Fifteen lambs with WMD and ten control animals were used as research materials. Blood samples were collected from both groups before- and 1 month after treatment for Se analysis by fluorimetry, whereas vitamin E and sialic acid were measured by HPLC and spectrophotometry, respectively. Compared to controls, in the diseased animals, there was a significant increase of serum total sialic acid (TSA) and LBSA, together with significant decreases of serum Se and vitamin E concentrations (p < 0.001). One month after treatment, a reversal of trend was observed with decreases of TSA and LBSA and increases of Se and vitamin E concentrations. The TSA and LBSA levels, however, remained significantly higher than those of the controls, p < 0.05 and 0.001, respectively. The Se and vitamin E concentrations of the treated animals were the same as those of controls. This is the first study on total and LBSA concentrations in lambs with WMD, showing that these markers can be used in the prognosis of the disease.