Browsing by Author "Yoruk, M"

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The Blood Parameters in Van Cats
(Scientific Technical Research Council Turkey, 1997) Cinar, A; Kilicalp, D; Yoruk, M
In this study, blood parameters of Van cats were investigated. Thirty healthy Van cats were used. Blood was taken from V. saphena of the cats. The means physiological blood parameters were as follows; red blood cell (RBC) 7.66 x 10(6)/mm(3), white blood cell (WBC) 11.75 x 10(3)/mm(3), eosinophil 0.600 x 10(3)/mm(3), and blood platelet 4.2 x 10(5)/mm(3). The mean hemoglobin concentration (Hb) and hematocrit value (PCV) were 12.1 g/dl, and 35.1% respectively. The mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were 46.2 mu(3), 15.9 mu mu gr, and 34.5 % respectively. The sedimentation rate was 4.0 mm/l hours in vertical situation. The mean erythrocyte diameter was 5.7 mu. The values of erythrocyte at he begining and en of hemolysis were 0.68% NaCl, and 0.50% NaCl respectively. The leukocyte formula was determined to be as follows; neutrophil 53%, eosionophil 7%, basophil 0%, lymphocyte 38% and monocyte 2%.
Effects of Cadmium Exposure on Morphological Aspects of Pancreas, Weights of Fetus and Placenta in Streptozotocin-Induced Diabetic Pregnant Rats
(Humana Press inc, 2003) Kanter, M; Yoruk, M; Koc, A; Meral, I; Karaca, T
This study was designed to evaluate the effects of Cd exposure on morphological aspects of beta-cell and weights of fetus and placenta in streptozotocin (STZ)-induced diabetic pregnant rats. Ninety-nine virgin female Wistar rats (200-220 g) were mated with 33 males for at least 12 h. From the onset of pregnancy, the rats were divided into four experimental groups (control, Cd treated, STZ treated, and Cd+STZ treated). The Cd-treated group was injected subcutaneously daily with CdCl2 dissolved in isotonic NaCl, starting at the onset of pregnancy throughout the experiment. Diabetes was induced on the 13th d of pregnancy by a single intraperitoneal injection of STZ in STZ-treated group. In addition to the daily injection of Cd, a single intraperitoneal injection of STZ was also given on the 13th d of pregnancy in the Cd+STZ-treated group. The rats received the last injection 24 h before being sacrificed and 10 randomly selected rats in each group were sacrificed on the 15th and 20th d of pregnancy. Blood samples were taken for the determination of the serum glucose and insulin levels. Maternal pancreases, fetuses, and placentas of sacrificed rats in all groups were harvested (fetal pancreas was also harvested only on the 20th d of pregnancy) for morphological and immunohistochemical examinations. Cd exposure alone caused a degeneration, necrosis, and weak degranulation, but Cd exposure with STZ caused a severe degeneration, necrosis, and degranulation in the beta-cells of the pancreatic islets. No morphological or immunohistochemical differences were found in beta-cells of fetal pancreatic islets of control or other treatment groups. Cd exposure alone also decreased the fetal and placental weights. The administration of STZ alone, on the other hand, increased the placental weight. Cd, STZ, and Cd+STZ administration increased the glucose and decreased the insulin level. The increase in glucose and decrease in insulin levels were higher when Cd and STZ were given together. All of these changes were more severe on the 20th d than those on the 15th d of the pregnancy. It is concluded that Cd exposure during pregnancy may reduce the birth and placental weights and produce necrosis, degeneration, and degranulation in beta-cells of pancreatic islets, causing an increase in the serum glucose level. These changes might be severe in diabetic pregnant mothers.
Effects of Zinc Toxicity on Thymus in Broilers
(indian veterinary Journal, 2003) Donmez, HH; Yoruk, M; Simsek, N
Light and Electron Microscopic Studies on Alpha Naphthyl Acetate Esterase Activity of the Peripheral Blood T Lymphocytes in Van Cats
(Wiley, 1998) Yoruk, M; Asti, RN; Kurtdede, N; Agaoglu, Z; Altunay, H
The purpose of this study was to determine the percentage of peripheral blood T Iymphocytes and the localization of ANAE enzyme at the electron microscopic level in Van cats by using an alpha-naphtyl acetate esterase procedure. Peripheral blood samples taken from 20 Van cats were used. The percentage of ANAE positive lymphocytes was 83.0%. Neutrophilic granulocytes gave a negative reaction, whereas monocytes, eosinophilic granulocytes showed a diffuse granular positivity. In the electron microscopic examination, ANAE positive reactions were seen in lysosomal granules found in lymphocytes.
Localization of Glycogen in the Placenta and Fetal and Maternal Livers of Cadmium-Exposed Diabetic Pregnant Rats
(Humana Press inc, 2003) Yoruk, M; Kanter, M; Meral, I; Agaoglu, Z
This study was designed to investigate the effects of Cd exposure on the glycogen localization in the placenta and in fetal and maternal livers in streptozotocin (STZ)-induced-diabetic pregnant rats. Ninety-nine virgin female Wistar rats (200-220 g) were mated with 33 males for at least 12 h. From the onset of pregnancy, the rats were divided into four experimental groups (control, Cd treated, STZ treated, and Cd+STZ treated). The Cd-treated group was injected subcutaneously daily with CdCl2 dissolved in isotonic NaCl, starting at the onset of pregnancy throughout the experiment. Diabetes was induced on d 13 of pregnancy by a single intraperitoneal injection of STZ in the STZ-treated group. In addition to the daily injection of Cd, a single intraperitoneal injection of STZ was also given on d 13 of pregnancy in the Cd+STZ-treated group. The rats received the last injection 24 h before being sacrificed and 10 randomly selected rats in each group were sacrificed on d 15 and d 20 of pregnancy. Blood samples were taken for determination of the serum glucose and insulin levels. Fetal and maternal livers of sacrificed rats in all groups were harvested on d 15 and d 20 of pregnancy, whereas placentas were harvested only on d 20 of pregnancy for histochemical examination. Although both Cd and STZ caused hyperglycemia and decreased insulin secretion, Cd-alone treatment increased the glycogen content only in the placental labyrinth, whereas STZ-alone treatment increased the glycogen content only in the maternal part of the placenta. Increased glycogen localization was observed in both the placental labyrinth and the maternal part of placenta when Cd and STZ were given together. Fetal and maternal livers of control and other treatment groups were not different regarding the glycogen content on d 15 or d 20 of pregnancy. It was concluded that Cd exposure during pregnancy might produce a glycogen localization in the placenta of diabetic rats. However, the function and the mechanisms of increased glycogen contents in the placenta of Cd-exposed pregnant diabetic rats remain unclear and further studies are needed.
A Rat Model for the Immune Response To the Intrauterine Administration of Bcg
(Scandinavian Federation Laboratory Animal Science, 2002) Kanter, M; Yoruk, M; Koc, A; Meral, I; Timurkan, HH
This study as designed to investigate the changes in the numbers of lymphocytes. macrophages and plasma cells in the uterus and ileocecal lymph nodes of rats exposed to the intrauterine administration of Bacillus-Calmette Guerin (BCG). Thirty female Wistar Albino rats. age 6 months and weighing between 200-250 g. ere assigned to the two experimental groups BCG treated and controls (n = 15). The intrauterine BCG injections where made using laparatomy in the diestrous cycle under Rompun and Ketalar anesthesia. 0.1 ml BCG ere injected for each into cornu uteri awhile the control group received 0.1 ml sterile saline in the same place, Two weeks later. the rats in both groups were anesthetized with ether and decapitated. Uterus and ileocecal lymph nodes were processed to determine alpha naphthyl acid esterase (ANAE) - positive T lymphocytes and macrophages. The plasma cells were stained with the methyl green-pyronin method. It was found that the numbers of lymphocytes. macrophages; and plasma cells on the uterus increased (P<0.01) in BCG treated rats. In addition. the number of these cells also increased in the ileocecal lymph nodes indicating the presence of an immune response to the intrauterine BCG administration. It is concluded that although the rat was chosen as a model and BCG as given by the process of laparatomy in this stud,,. intracervical administration of BCG in the uterus should be studied clinically in cases of immune deficiency disorders related to the uterus. such as endometritis. myometritis. pyometra. endometriosis. infertility and implantation problems of domestic animals. to see if there is an increase in the immune response.