Browsing by Author "Yucel, Ufuk Mercan"

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Analysis of Some Heavy Metals Accumulation in Water, Sediment and Tissues of Different Fish Species From the Razzaza Lake, Iraq
(Parlar Scientific Publications (p S P), 2019) Al-Jobory, Adel Abbas Safar; Yucel, Ufuk Mercan
In this study, some heavy metal accumulation were determined in water, sediment and different tissues of three fish species (Cyprinus carpio, Barbus grypus and Ctenopharyngodon idella) collected from Razzaza Lake. Water samples collected from Razzaza Lake were filtered and acidified with 5 ml concentrated nitric acid. Sediment samples and liver, muscle and gill samples were dissolved in the microwave by adding 1 ml of concentrated perchloric acid and 7 ml of concentrated nitric acid. Heavy metal levels in water, sediment and fish tissue samples were measured with ICP-MS instrument. Hg was not detected in lake water, sediment and fish tissues. Cd was not detected only in lake water. Among the metals, the highest concentrations in water, sediment and tissue samples were found for Al. The heavy metal levels detected in the Razzaza Lake water were below the permissible levels of heavy metals in the aquatic environment. According to Sedimanter quality guidelines, the Razzaza Lake sediment had the lowest effective concentration for As, Cu, Pb, Hg and Zn, high effective concentration for Cd and Ni. In the Razzaza Lake, heavy metal accumulation order has been identified as sediment> fish (liver> gill> muscle tissue)> lake water. Pb levels in all tissues and Ni levels in the gills of Ctenopharyngodon idella were above the permissible levels recommended by FAO and Turkish legislation. The present study shows that the metals level in muscle of Cyprinus carpio and Barbus grypus species did not exceed permissible levels recommended by FAO and Turkish legislation and these fish are considered safe for human consumption. But, the consumption of Ctenopharyngodon idella which is exposed to more heavy metals due to high consumption of grass should be restricted for human health.
Comparison of Sample Preparation Methods for Determination of Heavy Metals in Cattle Hair by Icp-Oes
(Parlar Scientific Publications (p S P), 2019) Yucel, Ufuk Mercan; Atasoy, Nurhayat
The purpose of this study is to compare the effectiveness of wet acid, surfactant and alkaline digestion procedures for determination of heavy metals in cattle hair samples. Cattle hair samples were collected from the tails of Holstein cattle and washed sequentially with acetone, distilled water, and acetone for removal of external contaminations. Analyses were carried out by ICP-OES after applying to acid digestion (HNO3:H2O2), acid digestion under addition of surfactant (Triton X-100), and alkaline digestion using Tetramethylammonium hydroxide. The accuracies of methods for Cu, Cr, Fe, Mn, Ni and Zn metals were evaluated by using NCS ZC 8100 2b hair certified reference material. The results showed that Cu, Fe results obtained by application of Triton X-100 and Cr, Fe, Ni and Zn results obtained by application of Tetramethylammonium hydroxide were not compatible with certified values, whereas wet acid digestion method provided satisfactory recoveries for all examined metals in cattle hair samples. Relative standard deviations were found below %5 in wet acid digestion method. The concentrations of Zn, Cr, Ni, Cu, Fe and Mn in cattle hair samples were in the range of 88-157, 0.94-3.22, 0.00-2.96, 14.4-33.0, 36-101, 0.20-3.00 mu g g(-1) respectively. Our results indicated that wet digestion method was the most efficient for the analysis of Cu, Cr, Fe, Mn, Ni, and Zn in the cattle hair sample with excellent precision and recovery.
Drug Interaction Between Ivermectin and Etomidate: Investigation on the Effect of Ivermectin on Etomidate Anaesthesia
(Soc Stiinte Farmaceutice Romania, 2020) Yucel, Ufuk Mercan; Oner, Ahmet Cihat; Turel, Idris; Yilmaz, Orhan
The present study aims to determine the effect of ivermectin on the duration of anaesthesia performed with etomidate and to find out if there are any interactions between these two drugs at the central nervous system level. For this purpose, 25 Sprague-Dawley female rats were divided into 5 groups with 5 rats in each group. Group I received only subcutaneous saline at a dosage of 0.1 ml/100 g bw. Group II received the intraperitoneal injection of propylene glycol at a dosage of 1 mL/100 g bw. Group III received the subcutaneously 0.2 mg/kg bw ivermectin. Two hours after these treatments, neuropharmacological tests (rotarod and open field) were performed. Also, Group IV received 20 mg/kg bw etomidate intraperitoneally. Group V received subcutaneously 0.2 mg/kg bw ivermectin and 2 hours after the ivermectin administration, 20 mg/kg bw etomidate was given intraperitoneally. In these two groups, anaesthesia was monitored and the sleep duration was calculated based on rectal reflexes. Once the animals were fully awake, neuropharmacological tests were performed. Ivermectin that was administered two hours before the etomidate anaesthesia prolonged etomidate sleeping duration strongly. Also, ivermectin increased motion and decreased motor coordination, in association with etomidate it potentiates CNS depression (with prolonged sleep duration and decreased motor coordination).
Heavy Metal Exposure of Workers Working at Petroleum Products Filling Stations and Its Effects on Oxidative Stress
(Parlar Scientific Publications (p S P), 2020) Atasoy, Nurhayat; Komuroglu, Ahmet Ufuk; Yucel, Ufuk Mercan; Kul, Ali Riza; Gursoy, Tulay
Petroleum products increase oxidative stress and inflammation due to its particulate content. This study aims to determine the levels of heavy metals (zinc, lead, cadmium, chromium) and to measure level of reduced glutathione and enzyme activity of superoxide dismutase in serum and hair specimens of workers (n=50, research group) employed in petroleum products filling stations in Van province. The control group (n=25) was selected working in the office and compared with the research group. Heavy metal levels in hair and serum specimens were determined by using the inductively matched plasma optical emission spectrometry instrument and serum enzyme levels were determined by using spectrophotometric methods. Zinc, lead, cadmium and chromium metal levels were higher in the research group than healthy controls. In addition, reduced glutathione level and superoxide dismutase enzyme activity decreased due to metal exposure and oxidative stress occurred in the research group. Smoking contributed to the exposure of amount of the metals. In research group, positive correlations were found between metal exposures and life span/term of employment. In addition, with the increased life span and term of employment, metal exposure increased and antioxidant enzyme levels decreased. This study shows that workers employed in stations are exposed to heavy metal toxicity and cause oxidative stress.
Use of an Antiarrhythmic Drug Against Acute Selenium Toxicity
(Elsevier Gmbh, 2020) Yucel, Ufuk Mercan; Basbugan, Yildiray; Uyar, Ahmet; Komuroglu, Ahmet Ufuk; Keles, Omer Faruk
Objective: Selenium is an essential trace element. But, selenium may have toxic effects in high doses. There are no proven antidotes or curative treatments for acut selenium toxicity. Treatment involves stopping the exposure and providing supportive care for symptoms. Therefore, it is necessary to find more effective substances in the treatment of selenium toxicity. The aim of this study was to increase the survival rate of animals by supporting the heart with amiodarone and to determine the effect of amiodarone on the pathological, hematological and biochemical parameters in acute selenium intoxication. Methods: 64 Wistar-Albino rats were divided into four groups. Group I was given only distilled water, Group II was given 18 mg/kg dose of amiodarone, Group III was given 18 mg/kg amiodarone and 10 mg/kg sodium selenite and Group IV was given sodium selenite 10 mg/kg (LD50 dose)orally. Results: 11 of the 16 animals in Group IV died within the first 48 h of drug administration. However, no deaths were observed in the rats in Group III. No hematological changes were observed. Biochemically, CK, CK-MB and LDH levels of Group IV were higher than the other groups on both the 2nd and 10th days. In Groups II and III, this serum level decreased, and vitamin B12 levels increased. In macroscopic inspections of the organs of Groups III and IV, slight paleness was detected. Histopathologically, degenerative changes in tissue were observed, especially in Group IV. Conclusion: This study shows that amiodarone application has a reducing effect on selenium toxicity. This was because amiodarone protected the heart by reducing CK and CK-MB levels and increased vitamin B12 levels, which play a role in the synthesis of S-adenosyl methionine that converts selenium into a nontoxic form.
Wound Healing Effect of Different Extracts of Centaurea Pterocaula
(Bangladesh Pharmacological Soc, 2019) Otun, Barus; Yucel, Ufuk Mercan
In this study, the effect of different extracts of Centaurea pterocaula were evaluated on 6 mm wound created by punch biopsy on the rat back. The leaves of the plant were powdered and extracted with distilled water, methanol, n-hexane and ethyl acetate. Extracts were obtained in the form of pomade with vaseline and applied topically to the wounds. The wound measurements performed on day 0, 2, 4, 6, 8, 10 and 12 and the histopathological examination performed on day 12. The n-hexane extract had an accelerating effect on wound healing and made a positive contribution to the healing process. In conclusion, C. pterocaula has a promising future for new drug development studies by its wound healing property.