Browsing by Author "Yuksek, V."

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The Correlation Between Neopterin, Myeloperoxidase and Oxidative DNA Damage in Sheep With Natural Babesiosis
(Selcuk Universitesi, Veteriner Fakultesi, 2024) Cetin, S.; Komuroglu, A.U.; Yuksek, V.; Dede, S.; Kilinc, O.O.; Yuksek, N.
Aim: The present study was designed to investigate the correlation between neopterin, myeloperoxidase (MPO), and DNA damage in sheep naturally infected with babesiosis. Materials and Methods: The study included 40 sheep Akkaraman, 1-3 years aged, with 20 healthy individuals (control group) and 20 naturally infected with babesiosis (patient group). Blood samples were collected from the jugular vein into biochemical tubes from all sheep. The cause of the disease was confirmed through smear tests and PCR analysis. Neopterin, myeloperoxidase (MPO), and oxidative DNA damage (8-OHdG) levels were measured using an ELISA method. Results: Neopterin and 8-OHdG levels were significantly lower in the control group compared to the patient group (P<0.05). Although myeloperoxidase were higher in the patient group, the difference was not statistically significant (P>0.05) Conclusion: In this study, a significant increase in neopterin and oxidative DNA damage, which are indicators of an immunological response, was observed in sheep with babesiosis. Neopterin in particular can be considered as an important marker of the immunological response to babesiosis. © 2024 Selcuk Universitesi, Veteriner Fakultesi. All rights reserved.
The Effects of Vitamin D Onto the Expression of Caspase Enzymes in Osteoblastic Cell Line Treated With Sodium Fluoride (Naf)
(Wiley, 2017) Yuksek, V.; Dede, S.; Taspinar, M.
Expression Levels of Some Apoptotic and Oxidative Genes in Sheep With Sarcocystosis
(Hellenic veterinary Medical Soc, 2022) Yuksek, V.; Kilinc, O. O.; Dede, S.; Cetin, S.; Ayan, A.
Sarcocystosis is a zoonotic protozoon-related disease with a very broad intermediate host spectrum. These protozoon parasites lead to tissue loss in their intermediate hosts. The purpose of this study was to present the mRNA expression levels of some genes belonging to the oxidative stress and apoptosis pathway systems in tissue damage caused by sarcocystosis. In this study, the material consisted of infected tissue taken from sheep esophagus determined to be sarcocystosis-infected and esophageal tissues taken from healthy sheep. The expression levels of the GPX1, SOD1, SOD2, NCF1and Nos2 genes that play a role in the oxidative stress mechanism and the caspase 3, 8, 9 and BCL-2 genes that play a role in the apoptosis mechanism were determined by RT-qPCR. As a result of the study, it was determined that, with increased oxidative stress, the gene expressions related to the relevant enzyme systems also increased, and in relation to this increase, the caspase enzyme genes that are effective in cell death were up-regulated. These results may shed light on similar studies for understanding and preventing damage mechanisms that may form as a result of sarcocystosis. As a result, it is understood that increased oxidative stress parameters and increased apoptosis in sarcocystic tissue in sheep cause tissue loss. We think that understanding the molecular mechanisms of this disease is clinically important in the treatment of parasitic diseases and in the prevention of economic losses that may occur as a result of the disease.
In Vitro Evaluation of the Apoptotic, Autophagic, and Necrotic Molecular Pathways of Fluoride
(Humana Press inc, 2021) Urut, F.; Dede, S.; Yuksek, V.; Cetin, S.; Usta, A.; Taspinar, M.
Prolonged exposure to high doses of fluoride causes chronic poisoning called fluorosis, which affects many tissues and causes serious health problems. This study was planned to investigate the apoptotic, autophagic, and necrotic molecular pathways of fluoride. Sodium fluoride (NaF) was administered to normal rat kidney epithelial (NRK-52E) cells. The NaF IC50 value was determined using the MTT assay. The expression of the genes in the autophagic, apoptotic, and necrotic pathways was determined by real-time PCR. It was determined that there were significant changes in NaF-induced molecular pathways depending on the time. There were no increases in apoptotic and necrotic pathway markers except for Atg3, an autophagy gene, at the 3rd and the 12th hours. However, there was an induction in all cell death signaling pathways at 24 h. The molecular mechanisms demonstrated NaF-induced cellular death in the NRK-52E cell line. It was concluded that these molecular mechanisms were activated with NaF, and different mechanisms accelerated the cellular death at the 24th hour.
In Vitro Evaluation of the Apoptotic, Autophagic, and Necrotic Molecular Pathways of Fluoride (Nov, 2020, 10.1007/S12011-020-02491-3)
(Springernature, 2021) Urut, F.; Dede, S.; Yuksek, V.; Cetin, S.; Usta, A.; Taspinar, M.
The Serum Protein Fractions in Streptozotocin (Stz) Administrated Rat Models
(EManuscript Services, 2017) Ragbetli, C.; Dede, S.; Koc, F.; Yuksek, V.; Ragbetli, M.C.
Background: Experimental diabetes can be induced using chemical agents such as streptozotocin Objective: This study aimed to investigate the effect of streptozotocin (STZ) which is most important agent to produce experimental diabetic model at two different doses on serum protein fractions in rat models. Materials and Methods: Twenty-four male rats that weighed an average of 250 g and were 3-4 months old were used as the experimental models. They were sorted into three groups composed of eight rats each of STZ 55 mg/kg, STZ 65 mg/kg and control. Diabetes was induced by administering STZ 55 mg/kg and 65 mg/kg intraperitoneally. The serum protein fractions were analyzed by cellulose acetate electrophoresis. Results: No significant difference was observed between the groups for all fractions except alpha-2 and beta globulins. The alpha-2 and beta globulin levels were significantly higher in the 55 mg/kg group than in the 65 mg/kg STZ and control groups (p<0.05). Conclusion: This increase may be due to the involvement of different proteins in the alpha-2 and beta globulin protein fractions. © 2016 Phcog.Net.
The Treatment of Thymoquinone Increased Expression Levels of Apoptotic and Oxidative Genes in the Nrk-52e Rat Kidney Epithelial Cell Line
(Suleyman Demirel University, 2023) Karaman, R.S.; Dede, S.; Yuksek, V.
This study was aimed to show the effects of Thymoquinone (TQ), the active ingredient of Nigella sativa, on apoptotic and oxidative pathways in kidney cells, depending on concentration and time. For this purpose, the NRK-52E rat kidney epithelial cell line was used. To determine the IC50 and proliferative values of TQ, the MTT cell viability test was performed at 24 and 48 hours. The proliferative (TQP-10µM) and toxic (TQIC50-60 µM) concentrations were determined. The expression of apoptotic (caspase 3 (CASP3), caspase 8 (CASP8), caspase 9 (CASP9), Bax (Bcl-2 associated X), Bcl-2 (Bcl-2 apoptosis regulator)) genes and oxidative (glutathione peroxidase 1(GPX1)), superoxide dismutase 1 (SOD1), neutrophil cytosolic factor 1 (NCF1)) genes were determined as concentration and time-dependent, in the samples taken by RT-qPCR at the concentrations and times dependent. According to the results of this analysis, GPX1 gene was significantly up-regulated in Tp compared to the control gene at 24 hours, SOD1 gene and NCF1 gene did not change in TIC50. Apoptotic genes were found to be limited up-regulated at both concentrations at 24 hours. At 48 hours, GPX1 was up-regulated at TQP concentration and SOD1 at TQIC50 concentration. The NCF1 gene was significantly expressed at TPIC50 at only 48 hours. Apoptotic genes were limitedly upregulated at TQP concentration at 48 hours, while significant upregulation of all apoptotic genes was found at TQIC50 concentration. In conclusion, GPx increased significantly at Tp at the 24th hour, SOD1 at TQIC50 concentration, and NCF gene increased significantly at the toxic concentration at the 48th hour. It was revealed that the effect of the apoptotic pathway on TQ-dependent cell death was limited at the 24th hour, while apoptotic cell death at toxic concentrations occurred externally at the 48th hour. © 2023 Suleyman Demirel University. All rights reserved.