Browsing by Author "Yuksek, Veysel"

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Apoptotic and Oxidative Mechanisms in Liver and Kidney Tissues of Sheep With Fluorosis
(Springernature, 2021) Efe, Ugur; Dede, Semiha; Yuksek, Veysel; Cetin, Sedat
This study was planned to determine the molecular basis and causes of damage to the kidney and the liver, which are the most affected tissues in sheep exposed to chronic fluoride. For this purpose, liver and kidney tissues were obtained from sheep with signs of fluorosis in the age range of 4-6 years. The control group consisted of clinically healthy sheep without fluorosis. The apoptotic and oxidative genes expression of target genes was determined using the real qRT-PCR method. According to the control gene (Gapdh) that was detected that in the liver, the apoptotic genes caspase-8, caspase-9, and Bim increased and caspase-3, Bcl-2, and Bak decreased, while in the kidney, caspase-3 and Bax and caspase-8, Bcl-2, Bcl2l-1, Bim, and Bak decreased. According to the 2-Delta Ct values of the oxidative stress genes, it was determined that Cygb, Gstp1, and Ncf1 genes increased significantly in the fluorosis group and Gpx1, sod1, and sod2 genes decreased significantly. In the kidney tissue, Cygb and Gpx1 increased in the fluorosis group, while sod1, sod2, Gstp1, Ncf1 and Ccs, and Nos2 were found to decrease significantly. As a result, it was shown that apoptosis and oxidative mechanisms are activated in the liver and the kidney tissues of sheep with fluorosis and these parameters have an important role in understanding the molecular basis of tissue damage in fluorosis.
April Cetina, Dede, Oto, Yuksek, Bulduk, the Effect of Resveratrol on Serum Protein Fractions in Rats Exposed To Experimental Chronic Fluorosis
(int Soc Fluoride Research, 2022) Cetin, Sedat; Dede, Semiha; Oto, Gokhan; Yuksek, Veysel; Bulduk, Mehmet; Ozdemir, Hulya
Chronic fluorosis results from long-term fluoride intake at more than the normal doses. The aim of this study was to demonstrate the effects of resveratrol (Res) on the serum protein fractions in rats, in which experimental chronic fluorosis was induced. After an adaptation period, the rats were randomly divided into 4 groups of 10; namely the (i) control, (ii) sodium fluoride (NaF), (iii) Res, and (iv) NaF+Res groups. Serum protein fractions in the rat blood samples were determined by cellulose-acetate electrophoresis. While the NaF group had statistically reduced concentrations of total protein, albumin, and alpha-1 and alpha-2 globulin compared to the control group (p < 0.05), these values were significantly increased (p < 0.05) in the NaF+Res group, compared to the NaF group, and close to those of the control group. The 0- and gamma-globulin concentrations were the lowest in the NaF group statistically (p < 0.05). Despite a significant increase (p < 0.05) in these values in the NaF+Res group, compared to the NaF group, they were still lower compared to the control group. The examination of the percentage of serum protein fractions revealed a reduced albumin in the NaF group compared to the control group but the finding was not statistically significant (p > 0.05). The albumin of the NaF+Res group was statistically higher than that of the control group (p < 0.05). No statistical differences were observed in alpha-1 and alpha-2 globulin across the groups. The 0 -globulin of the NaF group was the highest but not statistically higher than that of the control group. The gamma-globulin percentages in all the groups were found to be lower than the levels in the control group. The albumin/globulin (A/G) ratio decreased in the NaF group but was not significantly different than that of the control group. In conclusion, the alterations in the serum protein fractions due to NaF-induced toxicity, especially the alterations in their concentrations, approached values closer to those of the control group with the administration of resveratrol. We concluded that these results are of potential importance in indicating a favorable role for resveratrol use in preventing and treating fluoride toxicity.
Determination of Blood Serum Protein Fractions of Calves With Clinically Diagnosed Pneumonia
(Agricultural Research Communication Centre, 2021) Ekici, Pinar Tanritanir; Dede, Semiha; Yuksek, Veysel; Cetin, Sedat; Usta, Ayse
Background: Respiratory diseases in calves are causing worldwide economic losses for the beef industry. This study was planned to determine the serum protein fractions and A/G ratio in calves with clinically diagnosed pneumonia and evaluate the possibility uses of these parameters as clinical diagnostic parameters. Methods: The 34 calves with respiratory system problems and 10 healthy calves without clinical pneumonia symptoms were used as materials. The obtained serum samples were used for total protein and serum protein electrophoresis. Result: Albumin, alpha 1-globulin percentages and A/G ratio decreased in the patient group (p <= 0.01), other globulin fractions were higher in the patient group (p <= 0.01), for % g values. According to concentration results, it was found that while albumin did not show a significant difference, alpha 1-globulin (p <= 0.05) and A/G ratio (p <= 0.01) decreased in the patient group. In addition, other globulin fractions and total protein levels were higher in patient groups (p <= 0.01). As a result, the serum protein fractions should be evaluated as a useful biochemical blood parameter for diagnosis and follow up of lung diseases especially in calves.
Dna Damage-Induced by Sodium Flouride (Naf) and the Effect of Cholicalciferol
(Tech Science Press, 2020) Yuksek, Veysel; Dede, Semiha; Usta, Ayse; Cetin, Sedat; Taspinar, Mehmet
It is known that the high electronegativity of fluorine affects various soft tissues, especially the bone structure in organisms. Of these tissues are the kidneys, which play an important role in the excretion of fluoride from the body. Fluoride affects many cellular mechanisms. One of these effects is DNA damage. Our study aimed to investigate the likely protective effect of cholecalciferol (vitamin D3) on genomic DNA damage-induced NaF depending on concentration and time. The IC25 and IC50 values of NaF for 3, 12 and 24 h and optimum dose of increase in proliferation to vitamin D-3 through MTT assay in NRK-52E kidney cells were determined. DNA damage was significantly increased (p < 0.05) compared to the control group in all groups except for vitamin D-3 It was determined that treatment with NaF together with vitamin D-3 decreased the DNA damage compared to NaF treated groups for 3 and 12 h. NaF combined with vitamin D3 was determined statistically to decrease (p < 0.05) DNA damage compared to NaF treated groups for 24 h. As a result, it was determined that the treatment with cytotoxic concentration NaF depending on the time significantly increased (p < 0.05) the genomic DNA damage, but NaF treatment together with vitamin D-3 decreased the DNA damage in renal cells depending on the time. It was concluded that vitamin D-3 may be useful in preventing DNA damage caused by NaF.
The Effect of Lycopene on Dna Damage and Repair in Fluoride-Treated Nrk-52e Cell Line
(Springernature, 2021) Cetin, Sedat; Usta, Ayse; Yuksek, Veysel
Exposure of fluorine at toxic concentrations causes serious damage by accumulating in especially bones, kidneys, and other soft tissues. Fluorine at cytotoxic concentrations may cause DNA damage. This study aims to determine the level of DNA damage due to sodium fluoride (NaF) at different hours (3rd, 12th, and 24th hours) and in IC(50)concentrations designated for each hour and reveal the protective effect of lycopene on possible damage. The best enhancer concentrations (1 mu M) of microtitration (MTT) viability test and proliferation of lycopene and IC(50)values of NaF at the 3rd, 12th, and 24th hour were 9600, 5500, and 3200 mu M, respectively. DNA damage significantly increased in all NaF-treated groups in comparison with the control group (p < 0.05). DNA damage due to NaF+LYC application significantly decreased in comparison with the control group (p < 0.05). Lycopene application significantly increased the expression levels of the Ku70 and Ku80 genes which have a part in DNA repair (p < 0.05). The statistical data showed that application of lycopene which is an important antioxidant molecule may be beneficial for decreasing NaF-induced DNA damage. In conclusion, applying lycopene for cytotoxicity due to fluorine in NRK-52E cell line had different effects based on the dosage and time; thus, it can be a potential option for preventing fluorosis-induced toxicity and developing new treatment approaches.
The Effect of Quinoa (Chenopodium Quinoa) on Apoptotic, Autophagic, Antioxidant and Inflammation Markers in Glucocorticoid-Induced Insulin Resistance in Rats
(Springer, 2022) Erfidan, Siber; Dede, Semiha; Usta, Ayse; Yuksek, Veysel; Cetin, Sedat
Background Insulin resistance plays an important role in predicting type 2 diabetes that may develops. This study was planned in order to investigate the beneficial effects of quinoa (Chenopodium quinoa) use in glucocorticoid induced-insulin resistance. Methods and results Forty-two rats were used as the material (experimental) groups: the control group (C), the quinoa-administered group (Q), the insulin resistance-created group (IR), the IR + metformin group (IM), the IR + quinoa for treatment group (IQ) and the quinoa + IR for prophylaxis group (QI). Blood glucose, insulin levels and HOMA-IR were found to be highest (p < 0.05) in the IR group (p < 0.05). Glucose levels decreased significantly with the administration of quinoa and approached the levels of the control, but the insulin levels and the HOMA-IR did not significantly change. It was also observed that other biochemical parameters (ALT, AST, ALP, total cholesterol, total protein, urea and creatinine) changed significantly in the IR group and approached the levels of the control group with the administration of quinoa. Apoptotic (BCL2 5, BAX 9, CAS 3), autophagic (SQSTM1 7, ATG5) and inflammation (IL-1 beta, TNF-alpha) genes were upregulated by 5-11-fold in the IR group. In the groups in which quinoa was administered for treatment and protection, all these genes were found to be upregulated to a lower extent than the IR group. Antioxidant genes (GPX1, SOD1) increased by nine to tenfold in the quinoa groups. Conclusion As a result, after administration of quinoa, it was determined that the glucose level increased due to experimental insulin resistance and the liver and kidney damage indicators decreased. It was determined that quinoa (Chenopodium quinoa) had significant beneficial effects on biochemical parameters and apoptotic, autophagic, antioxidant and inflammatory markers in experimental glucocorticoid-induced insulin resistance.
The Effect of Vitamin E and Selenium Combination in Repairing Fluoride-Induced Dna Damage To Nrk-52e Cells
(Springer, 2020) Yuksek, Veysel; Cetin, Sedat; Usta, Ayse
Prolonged and excessive fluoride exposure can lead to fluorosis. The kidney is one of the organs that are injured mostly due to fluoride-induced damage. Fluoride can induce DNA damage at cytotoxic concentrations. This study aims to determine the extent of NaF-induced DNA damage and to investigate the effect of vitamin E and selenium combination (ES) in preventing and repairing this damage. For this purpose, we administered different combinations of NaF and ES to NRK-52E cells and determined the effective concentrations of ES and the NaF IC(50)values associated with different incubation times (3, 12, and 24 h) by using the MTT assay. The determined quantities of NaF IC(50)in association with time and the NaF IC50+ ES combination were administered to the cells. The extent of DNA damage was determined with the comet assay and the expression levels of the Ku70/80 and PARP-1 genes were determined with the RT-qPCR method. DNA damage significantly increased in all experimental groups compared to the control group (p < 0.05). It was found out that the NaF and ES combination statistically reduced the DNA damage compared to the damage observed in the NaF-treated groups (p < 0.05). Treatment of the ES combination significantly increased the expressions of Ku70 and Ku80 genes involved in DNA repair (p < 0.05). We concluded that vitamin E and selenium can potentially be effective in the repair of fluoride-induced DNA damage based on the results of this in vitro study. Our results may shed light on the prevention of DNA damage associated with fluorosis.
Effects of Plantago Major Extract on Serum Protein Fractions in Broiler Diet
(Agricultural Research Communication Centre, 2017) Bingol, Nuriye Tugba; Dede, Semiha; Karsli, Mehmet Akif; Yilmaz, Orhan; Turel, Idris; Yuksek, Veysel
The aim of this study was to determine the effects of Plantago major's (P.major) water extract added into broiler diets at different levels on serum protein fractions. A total of 112 Ross 308 broiler chicks were used in the study. Experiment consisted of control and 3 treatment groups with a 28 chicks within each group. Each experimental group was divided into four subgroups consisting of4 chicks. A basal (control) diet was prepared and three experimental diets were established by addition of P.major into basal diet; P.major 1 (5 g/kg feed), P.major 2 (10 g/kg feed), P.major 3 (15 g/kg feed). Broiler chicks were fed with these diets for 42 days ad libitum. Total protein levels of P.major 3 group were lower than other groups and controls. It was determined that the albumin percentages and levels became decreased in a significant ratio in the P.major 1 and P.major 2, (P<0,05), Alpha 1 globulin percentage and concentration were found significantly high in P.major 2 group than the other groups (P<0.05), Alpha 2 level and percentage in P.major 1 group was seen significantly higher than the control group, the A/G ratio in P.major 1 and P.major 2 was seen significantly lower than the control group. There was no significant difference between groups for beta and gamma globulins as percentage.
The Effects of Sodium Fluoride (Naf) Treatment on the Pi3k/Akt Signal Pathway in Nrk-52e Cells
(Springernature, 2022) Korkmaz, Riskiye; Yuksek, Veysel; Dede, Semiha
The effects of the element fluorine on the phosphoinositide-3-kinase-protein kinase B/Akt (PI3K/Akt) pathway has a significant role in regulation of intracellular molecular mechanisms. NRK-52E rat kidney epithelial cell line was selected as the material of the study. NaF was used as the fluorine source in the study. The NaF dose was determined with the MTT assay. The NaF concentrations were determined as the proliferation concentration of 10 mu M and IC25 (2250 mu M) and IC50 (4250 mu M) for 24 h. In the study, the erb-b2 receptor tyrosine kinase 2 (ERBB2), phosphoinositide-3-kinase (PI3K), Protein kinase B (PKB,Akt), Mammalian target of rapamycin (mTOR), and the Tumor protein 53 (TP53) genes were considered as the target genes. NaF concentration was administered on the cells. Total mRNA was isolated. mRNAs were turned into cDNA. The expression levels of the target genes were determined by RT-qPCR method. According to the results obtained in the study, the low NaF concentration increased the expression levels of the ERBB2, PI3K, and Akt genes, while the higher concentrations did not significantly affect these levels. The expression of mTOR decreased at all given concentrations. The expression of the TP53 gene did not change at the low concentration, while it increased at the high concentrations. Based on the results, it may be stated that fluorine may inhibit the kinase enzymes in the PI3K/Akt pathway. In summary, in the pathogenesis of the cell damage caused by fluorine in the NRK-52E cell line, the PI3K/Akt/mTOR pathway is an important signal pathway.
The Effects of Some Minerals on Apoptosis and Dna Damage in Sodium Fluoride-Administered Renal and Osteoblast Cell Lines
(int Soc Fluoride Research, 2019) Cetin, Sedat; Yur, Fatmagul; Taspinar, Mehmet; Yuksek, Veysel
The present study was planned to investigate the effects of some minerals (MgCl2, Na2SeO3, AlCl3 CaCl2) on the expression and translocation of certain apoptotic markers in NaF-administered (at the rate of IC50) rat renal epithelial (NRK-52E) and human osteoblast (hFOB 1.19) cell lines. The NaF IC50 and the non-toxic mineral doses were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. For the biochemical analysis, the cells were collected by trypsin treatment following a 24-hour incubation period, the cells were broken up by the freeze/thaw method, and the analysis was conducted. Caspase 9, 8, and 3 levels and gene expression, and M30 and 8-OHdG levels were determined. Target gene DNAs were propagated with the real time-PCR method. In the MTT studies, it was determined that the cell proliferation in rat renal epithelial cells (NRK-52E) treated with NaF+minerals was higher than that of all the NaF-treated groups and in the human osteoblast cells (hFOB 1.19), the cell proliferation was higher than in all the NaF-treated groups except for the MgCl2 group. The reason why the NaF administration in the NRK-52E cells resulted in an average of a 2-fold decrease in caspase 3 expression compared to the control group could be attributed to the apoptotic effect of NaF based on the time we obtained the RNA. However, based on this time, when the results are assessed based on the NaF and other mineral groups, the NaF-induced cytotoxic apoptosis might have used a pathway other than the apoptotic pathway. Thus, it is considered that minerals could usually prevent NaF-induced apoptosis by a synergistic mechanism due to the ionic character of NaF. NaF+mineral administration protected the NRK-52E cells from apoptosis. In the osteoblasts, on the other hand, it was concluded that NaF+mineral administration may be useful since it inhibits increased apoptosis.
The Effects of Thymoquinone on Dna Damage, Apoptosis and Oxidative Stress in an Osteoblast Cell Line Exposed To Ionizing Radiation
(Taylor & Francis Ltd, 2021) Yilmaz, Osman; Yuksek, Veysel; Cetin, Sedat; Dede, Semiha; Tugrul, Taylan
Ionizing radiation (IR) may induce oxidative-stress-related molecular changes (DNA damage, cell death, etc.) in living organisms by affecting them directly and indirectly in a time- and dose-dependent manner. This study was planned to present the effects of thymoquinone (TQ) in preventing oxidative stress, DNA damage and apoptotic cell death that may occur after IR exposure. For this purpose, hFOB 1.19 osteoblast cells were used. Using the MTT viability test, the dose of IR reducing the proliferation of cells and the concentration of TQ increasing cell proliferation the most were determined. Five groups were formed as the Control (C), Thymoquinone (TQ), Ionizing radiation (IR), Ionizing radiation + Thymoquinone (IR + TQ) and Thymoquinone + Ionizing radiation (TQ + IR) groups. By applying the determined concentrations on the cells, DNA damage level was determined with the Comet assay, and the gene expression levels of enzymes effective in the oxidative and apoptotic mechanisms were determined with the RT-qPCR method expression. It was determined that, as a result of IR application for 24 and 72 h, DNA damage occurred in the osteoblast cell line. While no significant change was observed in the oxidative and apoptotic gene expression levels at the end of 24 h, an increase in comparison to the control group was determined in the Sod-1 (2.4-fold), Caspase-3, and Caspase-9 gene expression levels at the end of 72h. . Application of TQ in 72 h of incubation before and after IR application significantly reduced the expression levels of Caspase-3, Caspase-9, and DNA damage together with the increased GPx-3 and Sod-1 gene expression.Consequently, it was seen that IR application affected apoptotic and oxidative stress-regulating genes in the hFOB 1.19 osteoblast cell line in a time- and dose-dependent manner, and it was thought that the harmful effects on this cell line may have been caused by the activation of the mitochondrial pathway. It was determined that TQ significantly reduced the gene expression values that changed due to IR and brought them close to those of the control group, and it was concluded that TQ became useful by showing antioxidant properties against the harmful effects of IR.
The Effects of Vitamin D Application on Naf-Induced Cytotoxicity in Osteoblast Cells (Hfob 1.19)
(Springernature, 2023) Dede, Semiha; Taspinar, Mehmet; Yuksek, Veysel; Cetin, Sedat; Usta, Ayse
This study was planned to evaluate the effect of vitamin D administration on cytotoxicity due to fluoride exposure in vitro. NaF (IC50) and vitamin D (proliferative) were applied to human osteoblast (hFOB 1.19) cells. The major genes of apoptotic, autophagic, and necrotic pathways were determined by RT-PCR. 2-Delta Delta Ct formulation was used for expression analysis. In the NaF group, caspase 3, Bax, Bad, Bak, Bclx, Atg3, Atg5, Atg6, pG2, LC3-I, LC3-II, RIP1, and RIP3 genes were increased (2.6-15 times). It was observed that the expressions of these genes approached the control when vitamin D was given together with NaF. The Bcl2 gene increased significantly (sixfold) with the effect of NaF, and was down-regulated to some extent with additional vitamin D administration, but still more than in the control. As a result, it was determined that apoptotic, necrotic, and autophagic pathways were activated as the molecular basis of the damage in the bone tissue, which was most affected by fluorine, and these genes were down-regulated and approached the control group with the addition of vitamin D. It was concluded that this is an important data to explain the molecular basis of the protective and therapeutic effect of vitamin D against fluorine toxicity.
The Effects of Vitamins A, D, E, and C on Apoptosis and Dna Damage in Sodium Fluoride-Treated Renal and Osteoblast Cell Lines
(int Soc Fluoride Research, 2017) Yuksek, Veysel; Dede, Semiha; Taspinar, Mehmet; Cetin, Sedat
This study was planned to investigate the effects of the antioxidant and protective vitamins A, D, E, and C, on the expression and translation of certain apoptotic markers in the NRK-52E and hFOB 1.19 cell lines treated with NaF at half the maximal inhibitory concentration (IC50) for 24 hours. The IC50 for NaF and nontoxic vitamin doses were determined by the MTT viability test. For the biochemical assays, cells were harvested by trypsinization and lysed by the freeze/thaw method. The levels and gene expression of caspases 3, 8, and 9, and the levels of M30 and 8-OHdG were also measured with methods that included the use of ELISA and qRT-PCR. In the MTT studies, compared to the NaF-treated groups, it was found that the cell viability was higher in all the NaF+vitamin D-treated groups in the NRK-52E cell line, in some of the NaF+vitamin D-treated groups in the hFOB 1.19 cell line, and in some of the NaF+vitamin A, E, and C-treated groups for both cell lines. In the NRK-52E cell line, the NaF IC50 value was determined and found not to induce apoptosis sufficiently so that it was considered that mechanisms other than the apoptotic pathways were instrumental in causing cell death. In the hFOB 1.19 cell line, it was observed that the apoptotic M30 protein level was increased in the NaF+vitamin D and NaF+vitamin C groups. In addition, in the hFOB 1.19 cell line, the qRT-PCR results showed that, while the expression of caspase-3 increased with vitamin A and that of caspase-8 increased with NaF, treatment with NaF+vitamin A led to a lower levels of caspases 3 and 8. Future studies to investigate the most valid and active mechanism for NaF-induced cell death and to elucidate the inhibitory-activating effects of vitamins on this mechanism using different doses, durations of exposure, and analytic methods should be considered.
The Electrophoretical Determination of Serum Protein Fractions in Lycopene Treated Experimental Diabetic Rats
(Humana Press inc, 2013) Yuksek, Veysel; Dede, Semiha; Ceylan, Ebubekir
This study was planned to determine the effects of lycopene treatment on serum protein fractions in experimental diabetic rats. In order to induce diabetes in rats in the diabetes (D) and diabetes + lycopene (DL) groups, rats were given 45 mg/kg single-dose streptozotocin intraperitoneally. Lycopene (10 mg/kg/day dissolved in sunflower oil) was administered to the rats in the lycopene-only (L) and DL groups. Blood glucose levels and HbA1c% in DL group and diabetes group increased (p < 0.05) compared to control and L group. Total protein, albumin, alpha(1), alpha(2), and beta globulin fractions of diabetic and DL groups were lower than control and L groups (p < 0.05). D group had lowest gamma (gamma) globulin levels among other groups (p < 0.05). The gamma globulin levels was slightly increased than diabetic groups (D and DL), but it was still lower than control and L groups (p < 0.05). The highest value of A/G ratio was observed in diabetic group. Similarly, the % level of A/G ratio of D group was higher than other groups. It was noted that the A/G ratio decreased and reached to control group levels after lycopene treatment.
In Vitro Evaluation of the Effects of Lycopene on Caspase System and Oxidative Dna Damage in High-Glucose Condition
(Wolters Kluwer Medknow Publications, 2019) Bazyel, Bunyamin; Dede, Semiha; Cetin, Sedat; Yuksek, Veysel; Taspinar, Mehmet
Aim and Background: The present study was planned to investigate the effects of lycopene, on the caspase-dependent apoptosis in high-dose glucose (HG)-treated PC12 cell line. PC12 cells were cultured in vitro. Materials and Methods: HG was prepared as G (250 mM), and lycopene was prepared as L1 (10 mM), L2 (20 mM), and L3 (40 mM). After 6 h of incubation, the cells were exposed to trypsin, and the samples were obtained with freeze/thaw method. Caspase 3, 8, 9; 8-hydroxy-2-deoxyguanosine (8-OHdG); and M30 were determined (enzyme-linked immunosorbent assay). Results: 8-OHdG increased in L3 (P % 0.001), whereas L1 caused a decrease in HG group (P % 0.001). Caspase-3 decreased significantly in L1, L2, and L3G compared to control (P % 0.001) group. Caspase-8 increased significantly in L1, L1G, L2G, and all L3 glucose groups (P % 0.001). There was no difference for Caspase-9. M30 was not affected by L and HG, which decreased significantly (P % 0.001). Conclusion: As a result, it was determined that, when PC12 cell line was treated with HG, lycopene application had effects on caspase enzymes and DNA damage.
In Vitro Evaluation of Thymoquinone and Lycopene Supplementation on Oxidative Dna Damage and Oxidant Status in High Glucose Conditions
(Colegio Farmaceuticos Provincia de Buenos Aires, 2019) Dede, Semiha; Yur, Fatmagul; Taspinar, Mehmet; Cetin, Sedat; Usta, Ayse; Yuksek, Veysel
The present study was planned to investigate the effects of thymoquinone (TQ) and lycopene (LYC), known to possess pro-inflammatory and antioxidant properties, on oxidative DNA damage (8-hydroxy-2-cleoxyguanosine) in BHK-21 cell line treated with high glucose (FIG) and the antioxidant system. BHK-21 cell line was cultured with regular passages (5% FBS, 10% host serum, 1% L-glutamine, 1% penicillin/streptomycin - RPMI 1640, 5% CO2 and 95%, 37 degrees C incubation). MTT cell viability tests were conducted. Proliferative TQ and LYC and glucose IC50 values were determined. Control, study groups; glucose (285 mM), TQ (10 mu M), and LYC (50 mu M)) and cross groups were designed. After incubation, trypsinized cells were broken by the freeze/thaw method and analyzed. Oxidative DNA damage, TAS, TOS and OSI values were determined for the obtained samples. It was determined that 8-OHdG levels were affected by high glucose (p <= 0.05), they increased further with the administration of TQ and LYC in addition to HG. TOS and OSI values increased in all study groups when compared to the control (p <= 0.05), and TAS levels significantly decreased (p <= 0.05) with the administration of HG when compared to TQ and LYC groups. In conclusion, TQ and LYC administration in addition to high glucose exacerbated oxidative DNA damage and OSI, and decreased TAS when compared to TQ and LYC groups. The TQ and LYC dose and administration duration in addition to high glucose in the present study led to an improvement in oxidative balance in the BHK cell line.
In Vivo Evaluation of Thymoquinone on Apoptosis and Oxidative Dna Damage in High Glucose Condition
(C M B Assoc, 2018) Gumus, Ali Furkan; Dede, Semiha; Yuksek, Veysel; Cetin, Sedat; Taspinar, Mehmet
The study was planned to investigate the effects of thymoquinone (TQ), which is a compound in N. sativa, on caspase dependent apoptosis and oxidative DNA damage in high glucose treated PC12 cells. PC12 cells were treated with high glucose (G1-150 mM, G2-250 mM, G3-350 mM), TQ (20 mu M), and their combinations. Oxidative DNA damage (8-OHdG (8-Oxo-2'-deoxyguanosine)), and apoptosis (caspase 3, caspase 8, caspase 9 enzymes and M30 protein) parameters were analyzed with ELISA. The 8-OHdG levels decreased in all combination groups compared to the control (p <= 0.001). There was no statistically significant difference between caspase 3 and 9. Caspase 8 in TQ, G3, TQG1, TQG2 groups were higher than the control (p <= 0.002). Low M30 levels were observed in TQG1 group (p <= 0.002). In conclusion, it was observed that in PC12 cell line treated with the high glucose concentrations, TQ administration had a statistically significant effect on oxidative DNA damage and some apoptotic parameters (caspase 8 and M30 protein).
Investigation of the Effect of Coenzyme-Q10 on Cyclophosphamide Induced Testicular Damage in Male Rats
(Univ Zulia, Facultad Ciencias veterinarias, 2023) Kosal, Volkan; Rua, Ihsan; Yuksek, Veysel; Keles, Omer Faruk
Cyclophosphamide (CP) is one of the frequently preferred chemotherapeutic agents Worldwide. CP has negative effects on the testes, spermatogenesis, and reproductive hormones. The aim of this study was to determine the protective effect of Coenzyme Q10 (CoQ10) on the damage caused by CP. CoQ10 is use in the treatment of infertility problems and is naturally found in the testes and seminal fluid. Thirty-six Albino Wistar male rats were divided into six groups ( Control, Sham, Cyclophosphamide (CP), Coenzyme Q10 (CoQ10), CP + CoQ10 I, CP + CoQ10 II), with six animals in each group. Semen analysis included investigations of sperm DNA damage, motility, abnormal sperm ratio, and density. Histopathological examination and assessment of oxidative stress parameters in the testes were conducted. Additionally, serum levels of FSH, LH, and Testosterone were measured. CoQ10 administration increased the motility rate, density, and Testosterone levels in testicular damage caused by CP (P<0.05). Furthermore, it was observed that the abnormal sperm ratio, sperm DNA damage, and oxidative stress were reduced (P<0.05). Based on the results of this study, the use of CoQ10 in conjunction with CP has the potential to alleviate male infertility problems that may arise from CP administration.
Lycopene Prevents Cell Death in Nrk-52e Cells by Inhibition of High Glucose-Activated Dna Damage and Apoptotic, Autophagic, and Necrotic Pathways
(Wiley, 2024) Usta, Ayse; Yuksek, Veysel; Cetin, Sedat; Dede, Semiha
This study aims to investigate the effects of lycopene on apoptotic, autophagic, and necrotic pathways, oxidative status, and DNA damage in diabetic nephropathy at the molecular level. The sample of the study includes seven groups: lycopene (L), high glucose (G), high glucose + lycopene (GL), and control (C) groups tested at 12 and 24 h. The expression levels of genes in oxidative, apoptotic, autophagic, and necrotic cell death pathways are determined by reverse transcription-quantitative polymerase chain reaction analysis. The comet assay method is used for the analysis of DNA damage. It is observed that adding lycopene to high glucose for protective purposes reduces the expression of genes related to apoptosis, autophagy, and necrosis, as well as the DNA damage index, compared to cells given high glucose alone. Lycopene can be a safe and effective alternative agent. In this study, where the molecular mechanisms were examined, it was concluded that lycopene, used as an agent of necrosis, autophagy and apoptosis inhibition, had beneficial effects on cells treated with high glucose. image
Phenolic Contents, Antioxidant Activities, Lcms Profiles of Mespilus Germanica Leaf Extract and Effects on Mrna Transcription Levels of Apoptotic, Autophagic, and Necrotic Genes in Mcf7 and A549 Cancer Cell Lines
(Humana Press inc, 2024) Gormez, Gul; Yuksek, Veysel; Usta, Ayse; Dede, Semiha; Gumus, Selcuk
Cancer, defined by the continuous, uncontrollable proliferation of cells in the human body, is a disease with a rapidly increasing incidence and mortality rate. Scientists are looking for novel ways to cure and prevent this sneaky disease because of the toxicity of contemporary chemotherapy and the cancer cells' resilience to anticancer drugs. Determining the effect of herbal medicines, which do not have as harmful side effects as synthetic drugs, on cancer cell lines is an essential preliminary study in the production of effective drugs against cancer. In this study, the phenolic acid profile, antioxidant capacity, and cytotoxicity of the medicinal plant Mespilus germanica (MG) leaf extract were determined, and its effects on the expression of some apoptotic, necrotic, and autophagic pathway genes of MCF7 (Human breast cancer line) and A549 (Human lung cancer line) and healthy HDF (Human Dermal Fibroblasts) cells were investigated for the first time. The LCMS device detected many important phenolic compounds previously reported to act against cancer cells in Mespilus germanica leaf extract. DPPH and total phenolic content showed high antioxidant capacity. The cytotoxicity of MG was determined by the MTT method. The levels of mRNA transcription for Atg5, Atg3, R & imath;pk1, Bcl2, Bax, Apaf1, Caspase-8, Caspase-7, Caspase-3, and Caspase-9, as well as the expression patterns of the DNA damage markers P53 and Parp-1 genes, were assessed. MG leaf extract did not cause significant toxicity against healthy HDF cells. However, it had a cytotoxic effect on A549 and MCF7 cancer cell lines, increasing the transcription levels of essential genes involved in cell death mechanisms. This research is the first to analyze the phenolic components and antioxidant capabilities of leaf extracts from Mespilus germanica. Additionally, it investigates the impact of these extracts on crucial genes involved in cell death pathways of A549 lung cancer, MCF7 breast cancer, and non-cancerous HDF (Human Dermal Fibroblasts) cells.