Browsing by Author "Yuksel, Mehmet Bilgehan"

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Dickkopf-1 Levels in Turkish Patients With Bladder Cancer and Its Association With Clinicopathological Features
(Asian Pacific Organization Cancer Prevention, 2014) Kaba, Mehmet; Pirincci, Necip; Benli, Erdal; Gecit, Ilhan; Gunes, Mustafa; Yuksel, Mehmet Bilgehan; Kemik, Ahu Sarbay
Background: Evidence indicates that Dickkopf-1 (DKK-1) levels may be a biomarker for cancer risk. The aim of this study was to assess DKK-1 and its correlation with clinic-pathological features in patients with bladder cancer. Materials and Methods: DKK-1 levels were determined in serum samples from 90 patients with bladder cancer before transurethral tumor resection. The concentrations of DKK-1 were determined by using enzyme linked immune-sorbent assay (ELISA). Results: Elevated preoperative DKK-1 levels were associated with tumor stage (p<0.001), grade (p<0.001) and histological grade (p<0.001). Conclusions: The results of our study demonstrated that the level of serum DKK-1 is correlated with both disease progression and increase in the tumor grade. Preoperative serum DKK-1 elevation may thus represent a novel marker for the determination of bladder cancer and the detection of patients with a likely poor clinical outcome.
Effect of Short-Term Treatment With Levosimendan on Oxidative Stress in Renal Tissues of Rats
(Sage Publications inc, 2014) Gecit, Ilhan; Kavak, Servet; Yuksel, Mehmet Bilgehan; Basel, Halil; Bektas, Hava; Gumrukcuoglu, Hasan Ali; Demir, Halit
The aim of this study is to evaluate the influences of short-term treatment with levosimendan (chemical formula: C14H12N6O) on oxidative stress and some trace element levels in renal tissues of healthy rats. A total of 20 male Wistar-albino rats were randomly divided into two groups, each consisting of 10 rats. Animals in the first group were not treated with levosimendan and served as control. Animals in the second group were injected intraperitoneally with 12 mu g/kg levosimendan and served as levosimendan group. Animals in both the groups were killed 3 days after the treatment, and their kidneys were harvested for the determination of tissue oxidant/antioxidant statues and trace element levels in renal tissues. The tissue malondialdehyde level was significantly (p < 0.001) lower in levosimendan group than in controls. The protective enzyme activities such as superoxide dismutase, catalase, and glutathione peroxidase and antioxidant glutathione level were significantly (p < 0.001) higher in levosimendan group than in controls. It was concluded that levosimendan reduced oxidative stress by avoiding lipid peroxidation and production of reactive oxygen species, and overactivating and/or increasing the protective antioxidant enzyme levels in renal tissues of rats. It is supposed that this experimental study provides beneficial data for clinicians in the management of renal tissue damage related to obstruction and/or ischemia.
Environmental Radioactivity and High Incidence Rates of Stomach and Esophagus Cancer in the Van Lake Region: a Causal Relationship
(Asian Pacific Organization Cancer Prevention, 2014) Akan, Zafer; Baskurt, Busranur; Asliyuksek, Hizir; Kam, Erol; Yilmaz, Ahmet; Yuksel, Mehmet Bilgehan; Koca, Dogan
This study examined the incidence rates of cancer cases (averages for 2006-2010) and relationships with environmental radioactivity levels. Soil and water samples were collected from provincial and district centers of Van city and the outdoor gamma doses were determined using a portable gamma scintillation detector. Gross alpha and beta, (226)Ra, (232)Th, and (40) K activities were measured in both tap water and soil samples. Although high rates of stomach and esophagus cancers have been reported previously in Van the underlying reasons have not hitherto been defined. Incidences of cancers were highest in the Gurpinar (326.0) and Ozalp (377.1) counties (p<0.001). As to the results of the gross alpha and gross beta radioactivity measurements in the drinking water, these two counties also had high beta radionuclide levels: Gurpinar (140 mBq/dm(3)) and Ozalp (206 mBq/dm(3)). Even if within the normal range, a relation between the higher rate of the incidence of stomach and esophagus cancers with that of the higher rate of beta radionuclide activity was clear. On Spearman correlation analysis, the relation between higher beta radionuclide levels and cancer incidence was found to be statistically significant (p<0.01). According to the results of the analysis, Van residents receive an average 1.86 mSv/y annual dose from outdoor gamma radiation, ingestion of radionuclides in the drinking water, and indoor Rn-222 activity. Moreover, gross alpha and beta activities were found to be extremely high in all of the lakes around the city of Van, Turkey. Further investigations with long-term detailed environmental radiation measurements are needed regarding the relationship between cancer cases and environmental radioactivity in the city of Van.
Haptoglobin Levels in Turkish Patients With Bladder Cancer and Its Association With Clinicopathological Features
(Asian Pacific Organization Cancer Prevention, 2012) Pirinicci, Necip; Gecit, Ilhan; Gunes, Mustafa; Kemik, Ahu Sarbay; Yuksel, Mehmet Bilgehan; Kaba, Mehmet; Aslan, Mehmet
Although alteration in the haptoglobin phenotype has been reported in patients with bladder cancer, serum haptoglobin levels have not been evaluated. We hypothesized that serum haptoglobin can be used as a biomarker. The aim of this study was to evaluate the expression of haptoglobin in bladder cancer and to determine the relationship with clinicopathological features. A total of 68 serum specimens obtained before surgery were used to investigate haptoglobin expression using the sandwich ELISA technique. Serum haptoglobin levels were higher in the patients with bladder cancer compared to healthy controls (p<0.0001). Additionally, the levels of haptoglobin protein increased with increasing tumor grades (p<0.001) and were significantly higher in patients with metastatic disease and the presence of lymphovascular involvement, lymph node metastases and increasing tumor burden (p<0.0001). This study suggests that elevated haptoglobin levels are associated with a higher stage, grade, and extent of distant metastasis and larger tumor size. Haptoglobin may therefore provide a useful diagnostic and treatment biomarker for patients with bladder cancer.
Levels of Serum Trace Elements in Renal Cell Carcinoma Cases
(Asian Pacific Organization Cancer Prevention, 2013) Pirincci, Necip; Gecit, Ilhan; Gunes, Mustafa; Kaba, Mehmet; Tanik, Serhat; Yuksel, Mehmet Bilgehan; Demir, Halit
Trace elements which are essential components of biological structures may also be toxic when present at levels above the amounts required for biological function. In our study, trace element levels were measured with furnace atomic absorption spectrophotometry in 33 newly diagnosed renal cell carcinoma cases (preoperative) and 32 healthy controls. When compared with the control group, it was found that the levels of cadmium (Cd), lead (Pb) were higher and the levels of zinc (Zn), iron (Fe) and manganese (Mn) were lower in the patient group. These changes may be important in the formation of renal cell carcinoma, a question which should be explored with postoperative comparative studies.
Preoperative Levels of Matrix Metalloproteinase-7 And-9 and Tissue Inhibitor of Matrix Metalloproteinase-1 Relation To Pathologic Parameters in Bladder Carcinoma Patients
(Asian Pacific Organization Cancer Prevention, 2013) Gunes, Mustafa; Kemik, Ahu Serap; Pirincci, Necip; Gecit, Ilhan; Taken, Kerem; Yuksel, Mehmet Bilgehan; Eryilmaz, Recep
Our aim was to test the hypothesis that preoperative serum levels of matrix metalloproteinase-7 (MMP-7) and -9 (MMP-9) and tissue inhibitor of matrix metalloproteinase (TIMP-1) levels correlate with pathological features. Serum levels of MMP-7, and MMP-9 and TIMP-1 were determined in 90 bladder cancer patients and 40 healthy controls using an enzyme linked immunosorbent assay. Preoperative serum MMP-7 and MMP-9 levels were significantly higher in cancer patients than control groups (p<0.001). In contast, serum TIMP-1 levels were lower (p<0.001). Alteration in MMP-7, and MMP-9, and TIMP-1 production may contribute to tumor angiogenesis and be associated with clinic-pathological features.
Serum Adenosine Deaminase, Catalase and Carbonic Anhydrase Activities in Patients With Bladder Cancer
(Elsevier Espana, 2012) Pirincci, Necip; Gecit, Ilhan; Gunes, Mustafa; Yuksel, Mehmet Bilgehan; Kaba, Mehmet; Tanik, Serhat; Aslan, Mehmet
OBJECTIVES: The relationship between adenosine deaminase and various cancers has been investigated in several studies. However, serum adenosine deaminase activity and carbonic anhydrase and catalase activities in patients with bladder cancer have not previously been reported. Therefore, the aim of this study was to measure serum adenosine deaminase, carbonic anhydrase and catalase activities in patients with bladder cancer. MATERIALS AND METHODS: Forty patients with bladder cancer and 30 healthy controls were enrolled in the study. Serum adenosine deaminase, carbonic anhydrase and catalase activities were measured spectrophotometrically. RESULTS: Serum adenosine deaminase, carbonic anhydrase and catalase activities were significantly higher in patients with bladder cancer than controls (all significant, p < 0.001). CONCLUSIONS: These markers might be a potentially important finding as an additional diagnostic biochemical tool for bladder cancer.
Serum Levels of Trace Elements in Patients With Prostate Cancer
(Asian Pacific Organization Cancer Prevention, 2014) Kaba, Mehmet; Pirincci, Necip; Yuksel, Mehmet Bilgehan; Gecit, Ilhan; Gunes, Mustafa; Ozveren, Huseyin; Demir, Halit
Background: Trace elements are major components of biological structures; however, excessive levels of these elements can be toxic. Materials and Methods: In the present study, serum levels of trace elements were measured in 30 patients with newly diagnosed as prostate cancer and 32 healthy volunteer by using furnace atomic absorption spectrophotometry. Results: It was found that there was an increase in serum levels of Co, Cu, Mg and Pb (p<0.05), whereas a decrease in serum levels of Fe, Mn, and Zn levels in patients with prostate cancer (p<0.05). Conclusions: These changes may be important in the pathogenesis of prostate cancers; however, further prospective studies are needed to identify relationships between prostate cancer and trace elements.
Serum Levels of Trace Elements in Patients With Testicular Cancers
(Brazilian Soc Urol, 2015) Kaba, Mehmet; Pirincci, Necip; Yuksel, Mehmet Bilgehan; Gecit, Ilhan; Gunes, Mustafa; Demir, Murat; Demir, Halit
Introduction: Trace elements are primary components of biological structures; however, they can be toxic when their concentrations are higher than those needed for biological functions. Materials and Methods: In the present study serum levels of trace elements were measured in 30 patients (mean age was 26.9 +/- 11.2 years) newly diagnosed with germ cell testicular cancer and 32 healthy volunteers (mean age: 27.4 +/- 10.8) by using furnace atomic absorption spectrophotometer. Serum samples were stored at-20 degrees C until assays. Results: In patients with germ cell testicular cancer, the diagnosis was seminoma in 15, mix germ cell tumor in 7, embryonal carcinoma in 4, yolk sac tumor in 2 and teratoma in 2 patients. There was stage I testicular tumor in 19 patients (63.3%) while stage II in 6 patients (20.0%), stage IIIA in 4 patients (13.3%) and stage IIIC in one patient (3.4%). It was found that serum Co, Cu, Mg and Pb levels were increased (p<0.05), whereas Fe, Mn, and Zn levels were decreased in patients with testicular cancer (p<0.05). Conclusions: These alterations may be important in the pathogenesis of testicular cancers; however, further prospective studies are needed to identify the relationship between testicular cancer and trace elements.
Serum Prolidase Activity, Oxidative Stress, and Antioxidant Enzyme Levels in Patients With Renal Cell Carcinoma
(Sage Publications inc, 2016) Pirincci, Necip; Kaba, Mehmet; Gecit, Ilhan; Gunes, Mustafa; Yuksel, Mehmet Bilgehan; Tanik, Serhat; Demir, Halit
Objectives: Prolidase is a member of the matrix metalloproteinase family. It plays a vital role in collagen turnover, matrix remodeling, and cell growth. Reactive oxygen species (ROS) have been implicated in the pathogenesis of various diseases, including cancers. Oxidative stress can cause tumor angiogenesis and may be carcinogenic. However, the relationship between antioxidant capacity and various cancers has been researched in several clinical trials. In our study, we aimed to identify serum prolidase activity, oxidative stress, and antioxidant enzyme levels in patients with renal tumors and to evaluate their relationships with each other. Materials and Methods: A total of 37 male patients with renal cell cancer and with a mean age of 56.28 +/- 3.1 were included in the study. The control group comprising 36 male patients (mean age 56.31 +/- 2.9) was randomly selected among the volunteers. Serum samples for measurement of superoxide dismutase (SOD), glutathione peroxidase (GSHPx), glutathione-S-transferase (GST), malondialdehyde (MDA), glutathione (GSH), and prolidase levels were kept at -20 degrees C until they were used. Results: Serum prolidase activity and MDA levels were significantly higher in renal cancer patients than in controls (all, p < 0.05), while SOD, GSHPx, and GST levels were significantly lower (p < 0.05). Conclusion: Our results indicate that increased prolidase seems to be related to increased oxidative stress along with decreased antioxidant levels in renal cancer.