Browsing by Author "Yunusoglu, O."

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Anti-Inflammatory Potential of Thymoquinone in Tumor Necrosis Factor-Alpha Stimulated Sw982 Human Synovial Fibroblasts
(Yuzuncu Yil Universitesi Tip Fakultesi, 2023) Berkoz, M.; Yunusoglu, O.; Krosniak, M.; Francik, R.
Rheumatoid arthritis is a common systemic autoimmune disease characterized by chronic inflammation of the joints that can induce the formation of pannus tissue and ultimately leads to joint destruction. Thymoquinone, the major bioactive constituent of Nigella sativa seed oil has diverse pharmacological properties. Although there are some studies in the literature showing the anti-inflammatory activity of thymoquinone, it is not yet clear whether thymoquinone can prevent inflammation caused by rheumatoid arthritis. The goal of this study was to investigate the potential anti-inflammatory effects of thymoquinone treatment on synovial fibroblast s. In our study, we investigated the effects of thymoquinone on nitric oxide production, interleukine-6 (IL-6), IL-8, and prostaglandin E2 (PGE2) levels, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor receptor-1 (TNF-R1), and TNF-R2 protein expressions, extracellular signal-regulated protein kinases 1 and 2 (ERK1/2), Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (MAPK), and Akt phosphorylation levels in tumor necrosis factor-alpha (TNF-α) stimulated SW982 human synovial fibroblasts. Thymoquinone treatment (0-1 µM) resulted in significant and concentration-dependently reduced the TNF-α stimulated production of nitric oxide, IL-6, IL-8, and PGE2 levels compared to the untreated group (p<0.05). Also thymoquinone treatment in high concentrations exerted an anti-inflammatory effect by suppressing iNOS, COX-2, TNF-R1, and TNF-R2 protein expressions and the phosphorylation of JNK, p38 MAPK, ERK1/2 and Akt in SW982 synovial fibroblasts (p<0.05). Taken together, these results show that thymoquinone in high concentrations is able to play a beneficial role in TNF-α mediated signaling in rheumatoid arthritis synovial fibroblasts. © 2023, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
In Vitro Anti-Obesity Effect of Aloe Vera Extract Through Transcription Factors and Lipolysis-Associated Genes
(Yuzuncu Yil Universitesi Tip Fakultesi, 2022) Yunusoglu, O.; Türkmen, Ö.; Berkoz, M.; Yıldırım, M.; Yalın, S.
In recent years, obesity has been associated with heart diseases, type 2 diabetes, obstructive sleep apnea, certain types of cancer and osteoarthritis, and become a global health problem. Therefore, researchers seek to find functional drugs against obesity. For the past few decades, medicinal plants have been examined to for their anti-obesity effects, including Aloe vera. In this study, it was aimed to elucidate the inhibitory effect of Aloe vera extract on adipogenesis. Firstly, 3T3-L1 pre-adipocytes were stimulated so as to differentiate into mature adipocyte using adipogenic differentiation cocktail consisting of 10 μg/mL insulin, 0.5 mM isobutylmethylxanthine, 0.25 mM dexamethasone, and 100 ng/mL biotin on day 0. Various concentrations (10-50 µg/mL) of Aloe vera extract with no cytotoxic effect were applied to differentiated 3T3-L1 cells. Glyceraldehyde-3-phosphate dehydrogenase (GPDH) activity, Oil r ed O staining, intracellular triglyceride levels, and gene expressions of transcription factors and lipolysis-associated genes were examined in order to investigate the effect of Aloe vera extract on adipocyte differentiation. Aloe vera treatment caused a continuous decrease in cell size and intracellular triglyceride accumulation. Despite the fact that GPDH activity, mRNA levels of transcription factors and lipolysis-associated genes decreased in mature adipocytes treated with Aloe vera extract. These results suggest that Aloe vera may have a therapeutic effect in prevention and/or treatment of adipogenesis-related obesity. © 2022, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Phytochemical Profile and in Vitro and in Vivo Anticonvulsant and Antioxidant Activities of Epilobium Hirsutum
(Pamukkale University, 2020) Dzhafar, S.S.; Dalar, A.; Mukemre, M.; Ekin, S.; Yıldız, D.; Yunusoglu, O.
This study presents the phytochemical profile and in vitro and in vivo anticonvulsant and antioxidant activities of Epilobium hirsutum, which has been traditionally used in the treatment of epilepsy by local people of Turkey. In vitro studies revealed that the extract contained a pronounced amount of phenolics (206.3±0.9 mg Gallic acid Eq/g extract) and exhibited significant levels of antioxidant (FRAP; 6226 µmol Fe2+/g extract, ORAC; 6593 µmol Trolox Eq/g extract, DPPH; IC50:33.8 ug/mL and metal chelation; IC50:114 ug/mL) and anticonvulsant (AChE; IC50:71.2 ug/mL, BChE; IC50:92.5 ug/mL, GABA-T; IC50:94.7 ug/mL) activities. In vivo studies shown that the extract exhibited high anticonvulsant activities. In addition, the extracts regulated the behaviour, locomotion and mental activities of the mice tested. Biochemical evaluation of the brain tissue revealed that the extract inhibited the production of MDA and stimulated the increasing of antioxidant enzyme levels, which suggest the possible antioxidative role of the extract that worked as neuroprotective agents by scarfing the free radicals produced through PTZ seizure inducer and attenuate convulsions. Moreover the extract regulated serum biochemical parameters, total antioxidant, total oxidant, and ischemia-modified albumin levels. Chromatographic studies were revealed that gallic acid principally might be the major contributor of anticonvulsant and antioxidant activities with the additive contributions of fatty acids and mineral compounds. Findings obtained from this study partially justified the traditional use of Epilobium hirsutum in the treatment of epilepsy and suggest potential use of the extract as industrial or pharmaceutical agent. © IJSM 2020.