Browsing by Author "Yur, Fatmagul"

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The Concentration of Certain Trace Elements in The Wool of Sheep With Fluorosis
(int Soc Fluoride Research, 2020) Cetin, Sedat; Deger, Yeter; Dede, Semiha; Yur, Fatmagul
The aim of this study was to evaluate the trace element levels in the wool of sheep, with and without fluorosis, living in a volcanic area of Turkey. Fifteen Akkaraman sheep with fluorosis in the Agri region, to the north of Lake Van, and 10 Akkaraman sheep without fluorosis in the Van region, to the south of Lake Van in the eastern part of Turkey, were investigated. The urinary fluoride levels were measured with an ion selective electrode. The sheep with fluorosis were identified by clinical examination and the presence of a high urinary fluoride level. The wool samples were obtained from the neck region. The trace element concentrations in these samples were measured using an atomic absorption spectrophotometer. It was determined that in the fluorosis group, compared to the control group, the copper and zinc levels were significantly decreased (p=0.05), and a non-significant decrease (p=0.05) was present for the levels of the nickel, manganese, iron, and cobalt.
Deneysel Diyabet Modelinde Solanum Nigrum Ekstraktının Antioksidan ve Antihiperlipidemik Etkisi
(2023) Fethullah, Mohammed Nooraddin; Oner, Ahmet Cıhat; Yur, Fatmagul
Diabetes mellitus (DM), kronik, metabolik, bulaşıcı olmayan bir hastalıktır, dünya çapında beşinci ölüm nedeni olarak kabul edilir ve dünya çapında epidemik oranlara ulaşmıştır. Çalışmamızda kontrol grubu (K), diyabet grubu (D), Solanum nigrum özü (SN) ve diyabet + Solanum nigrum özü (D+SN) verilen gruplar kullanılarak Solanum nigrum ekstraktının diyabetik etkilerini araştırmayı amaçladık. Sonuçlarımız, Solanum nigrum ekstraktının glukoz seviyeleri üzerinde biyolojik etkinliği gözlenirken, serum glukoz seviyesinde (D) (663±21.8 mg/dL) C'ye (131±9.8 mg/dL) kıyasla anlamlı artış kaydedildi. Ancak C grubu (131±9.8 mg/dl) ve SN grubu (196.14±12.1 mg/dL) arasında glukoz düzeyi açısından anlamlı fark yoktu. Ayrıca D + SN grubunun glukoz düzeyi (484.8±40.0 mg/dL), C (131±9.8 mg/dl), D (663±21.8 mg/dl) ve SN gruplarına (196.14±12.1 mg/dL) göre anlamlı olarak yüksekti). D grubunda (1,85±0,15,7) toplam antioksidan durum (TAS) düzeyi, C grubu (1.28±0.17) ile karşılaştırıldığında anlamlıydı. D grubu ile D+SN grubu arasında anlamlı farklar gözlendi (154±007). Ancak TAS düzeyleri hem SN (1.27±0.10) hem de D+SN (1.54±0.07) gruplarında kontrole göre anlamlı farklılık göstermedi. D grubunda (6.30±1.41) toplam oksidan durum (TOS) düzeyi, kontrol C (3.87±0.34), SN (4.87±0,80) grubu ve D+SN (4.14±0.34) gruplarına göre anlamlı farklılık gösterdi. Aksine, tüm C, SN, D+SN grupları arasında anlamlı bir fark yoktu. Sonuç olarak Solanum nigrum bitki ekstraktının diyabet üzerinde etkili olduğunu ancak glikoz seviyesini normal seviyelere indiremediğini, farklı dozlarda ve farklı ekstraksiyon yöntemleri ile etkilerinin ileriki çalışmalarda araştırılması gerektiğini söyleyebiliriz.
Doksorubisin Uygulanan Ratlarda Coq10 ve Vitamin E’nin Kanda Lipit Peroksidasyonu, Bazı Antioksidan ve Biyokimyasal Parametrelere Etkisi
(2021) Ceylan, Ebubekir; Yaşar, Semih; Yur, Fatmagul
Bu çalışmada doksorubisin uygulanan ratlarda, Koenzim $Q_10$ ($CoQ_10$) ve vitamin E’in lipitperoksidasyonu, antioksidan profil ve bazı biyokimyasal parametreler üzerine etkisi araştırıldı.Çalışmada altı aylık 40 adet Wistar-Albino ırkı erkek rat kullanıldı. Hayvanlar dört gruba ayrıldı.Birinci grup ratlara doksorubisin 2.5 mg/kg/serum fizyolojik i.p. olarak haftada bir kez 6 haftasüreyle uygulandı. İkinci grup ratlara doksorubisine ilave olarak $CoQ_10$ 4 mg/kg/canlı ağırlıkoranında i.p. olarak hergün uygulandı. Üçüncü grup ratlara sadece $CoQ_10$ 4 mg/kg/canlı ağırlıkoranında yine günlük olarak verildi. Dördüncü grup ratlara doksorubisine ilave olarak vitamin E 10mg/kg/subkutan/haftada iki kez 6 hafta süreyle uygulandı. Çalışma süresi altı hafta olarakplanlandı. Çalışmaya başlamadan önce ratlardan alınan kanlar kontrol grubu oluşturmak amacıylakullanıldı. Altıncı hafta sonunda tüm ratlardan kan örnekleri alındı. Analizler sonucunda tümgruplarda malondialdehit ve seruloplazmin düzeylerinde önemli artışlar gözlendi. Tüm gruplardaglutatyon düzeylerinde kontrol grubuna göre düşüşler gözlendi. Retinol ve α-tocopheroldüzeylerinde farklılık gözlenmedi. Albumin düzeylerinde önemli azalmalar ve globülin düzeylerindeönemli artışlar belirlendi. Total protein düzeylerinde önemli artışlar, alanin aminotransferazdüzeylerinde DXR grubunda önemli bir artış diğer guruplarda düşüşler gözlendi. Glukozdüzeylerinde ise belirgin düşüşler görüldü. Çalışmada DXR’nin oksidatif etkisi belirgin bir şekildegörüldü ancak verilen $CoQ_10$ ve vitamin E’nin antioksidan etkileri istenilen düzeyde belirgin olarakgözlenemedi. Çalışma verilerine göre $CoQ_10$ ve vitamin E gibi antioksidan maddelerin, çeşitliilaçların neden olduğu stres ve hasarı azaltmak için kullanılabileceği ancak verilen ilaçların kullanımsüresinin antioksidan maddelerin başarısını azaltabileceği sonucuna varıldı.
The Effect of Coq10 and Vitamin E on Serum Total Sialic Acid, Lipid-Bound Sialic Acid, Some Trace Elements and Minerals in Rats Induced With Doxorubicin
(Academic Journals, 2011) Oto, Gokhan; Ekin, Suat; Yasar, Semih; Ozdemir, Hulya; Yur, Fatmagul
This study was designed to evaluate the effect of CoQ(10) and vitamin E on serum total sialic acid (TSA), lipid bound sialic acid (LSA) and some elements in rat administered doxorubicin (DXR). Cu levels were increased in the group treated with DXR + vitamin E in comparison with DXR (p<0.05) and CoQ(10) groups (p = 0.001). Furthermore, copper levels were increased in the group treated with DXR + CoQ10 in comparison with CoQ(10) group (p < 0.05). Zn levels were decreased in the group treated with DXR + vitamin E in comparison with CoQ(10) group (p < 0.05). Mg levels were decreased in subjects treated with DXR + vitamin E in comparison with the control group values (p < 0.05). Particularly, the observed increase in Cu levels in rats from DXR + vitamin E group might be due to the decrease of vitamin E. However, the oxidative damage could be as a result of DXR occurence and may be helpful to clinicians in chemotherapy using anthracycline.
The Effect of Glutathione Treatment on the Biochemical and Immunohistochemical Profile in Streptozotocin-Induced Diabetic Rats
(Springer, 2013) Yur, Fatmagul; Dede, Semiha; Karaca, Turan; Yegin, Sevim Ciftci; Deger, Yeter; Ozdemir, Hulya
This study investigated the possible role of glutathione (GSH) in diabetic complications and its biochemical safety in experimental diabetic rats. Serum biochemical parameters and the histology of the pancreas were investigated. Seven rats were separated as controls. To create the diabetes in rats, 45 mg/kg single-dose streptozotocin (STZ) was administered i.p. The treatment was continued for 1 month. STZ was administered to the diabetes + GSH group, then reduced GSH, dissolved in isotonic salt solution (200 mg/kg), was applied i.p. two times a week. The GSH group received i.p. GSH. Serum biochemical parameters were determined by autoanalyzer. Immunohistochemical procedures were used to determine the percentage of the insulin-immunoreactive beta-cell area in the islets of Langerhans. The biochemical parameters changed to different degrees or did not change. Pancreatic cells of the control and GSH groups were healthy, but in the diabetic and GSH-treated diabetic groups we found damage in different numbers. The results from these analyses show that GSH supplementation can exert beneficial effects on pancreatic cells in STZ-induced diabetic rats and can safely be used for therapy in and protection from diabetes and complications of diabetes.
Effect of Lycopene Application in Rats With Experimental Diabetes Using Lipoprotein, Paraoxonase and Cytokines
(Springer, 2013) Yegin, Sevim Ciftci; Yur, Fatmagul; Ceylan, Ebubekir
This study was conducted with the purpose of researching the effect of lycopene application on lipoprotein, paraoxonase (PON) and cytokines that are projected to be used in the diagnosis and treatment of diabetes by making experimental diabetes. At the end of a 1-month trial period, under ether anesthesia with jelly tubes, blood samples were taken from rat hearts. Blood samples were centrifuged and serum was obtained. From the serum samples, HbA(1c), paraoxonase activity, lipoprotein levels and cytokines were determined. HbA(1c) levels and PON activity were found to be p < 0.001. At the triglyceride level, with regard to the control group, in all the groups a significant rise occurred (p a parts per thousand currency sign 0.001). At the cholesterol level, with regard to the control group, a decline was observed in the other groups (p < 0.05). At the VLDL level, with regard to the control group, a significant rise was observed in the other groups (p < 0.05). At the HDL (p < 0.001) and LDL (p < 0.05) levels, with regard to the control group, a significant decline was observed in the other groups. At the TNF-alpha, IL-2, IL-6 and IL-10 levels no difference was found (p > 0.05). Experimental diabetes models have an important place in analyzing diabetes complications and determining treatment approaches.
Effect of Lycopene on Serum Nitrite-Nitrate Levels in Diabetic Rats
(Medknow Publications & Media Pvt Ltd, 2015) Yegin, Sevim C.; Yur, Fatmagul; Cetin, S.; Guder, A.
The effect of lycopene on serum nitrate-nitrite levels was investigated in diabetic rats. In this investigation, 28 Wistar rats were divided into 4 groups, each of seven rats. These groups were control group, diabetes group, diabetes-lycopene group and lycopene group. The concentration of nitrite and nitrate was detected at high levels in diabetes group, diabetes-lycopene group and lycopene group as compared with the control group (P < 0.05). Especially, the increase in the levels of nitrate in diabetes group and lycopene group was statistically significant when compared with diabetes-lycopene group and control group (P < 0.05). In addition, we also determined the proportion of nitrite/nitrate for nitric oxide radical formation. Therefore, it is important to investigate the recovery and stability of nitrite and nitrate in samples. As a result of this study, it was observed that the amounts of nitrate and nitrite increased due to oxidative stress in diabetes and also application of antioxidant lycopene caused an increase in the amounts of nitrate and nitrite levels.
The Effect of Vitamin C and Vitamin E on Dna Damage, Oxidative Status, and Some Biochemical Parameters in Rats With Experimental Fluorosis
(int Soc Fluoride Research, 2020) Oner, Ahmet Cihat; Dede, Semiha; Yur, Fatmagul; Oner, Aysegul
Excess fluoride intake may lead to metabolic, functional, and structural damage in soft tissue and organs as well as in teeth and bones. The aim of the present study was to investigate the protective and therapeutic properties of vitamins C (vit C) and E (vit E) on the oxidative status and balance, DNA damage, and some biochemical parameters in rats with experimentally-induced chronic fluorosis. Seventy-two Wistar-Albino rats were divided into 9 groups, each containing 8 rats. Control groups were designed as real control and vehicle control groups. Vit C (100 mg/kg), vit E (300 mg/kg) and vit C + vit E (100 mg/kg + 300 mg/kg, respectively) in water that contained 150 ppm NaF were administered daily to three protection groups over 16 weeks. Three treatment groups received ad libitum water with 150 ppm NaF for 16 weeks, followed by the administration of normal drinking water plus vit C (100 mg/kg), vit E (300 mg/kg), and vit C + vit E (100 mg/kg + 300 mg/kg, respectively) on alternate days for 4 weeks. Serum biochemistry, total antioxidant status (TAS), total oxidant status (TOS), and analyses were conducted on sera obtained at the end of the study. To determine DNA damage, the comet assay and 8-hydroxy-2'-deoxyguanine (8-OHdG) analysis were used. Although the therapeutic and protective vitamin administration of vitamins C and E to the rats with experimentally-induced chronic fluorosis had only a limited effect on the oxidative status, the biochemical results for renal and hepatic function showed that the vitamins had positive effects and the comet assay and 8-OHdG analysis showed that DNA damage was reduced. In conclusion, significant positive results were obtained with protective and therapeutic treatment with vitamins E and C in rats with experimentally-induced chronic fluorosis. It is suggested that further studies are conducted to clarify the molecular mechanisms involved in producing these positive outcomes.
The Effects of Some Minerals on Apoptosis and Dna Damage in Sodium Fluoride-Administered Renal and Osteoblast Cell Lines
(int Soc Fluoride Research, 2019) Cetin, Sedat; Yur, Fatmagul; Taspinar, Mehmet; Yuksek, Veysel
The present study was planned to investigate the effects of some minerals (MgCl2, Na2SeO3, AlCl3 CaCl2) on the expression and translocation of certain apoptotic markers in NaF-administered (at the rate of IC50) rat renal epithelial (NRK-52E) and human osteoblast (hFOB 1.19) cell lines. The NaF IC50 and the non-toxic mineral doses were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. For the biochemical analysis, the cells were collected by trypsin treatment following a 24-hour incubation period, the cells were broken up by the freeze/thaw method, and the analysis was conducted. Caspase 9, 8, and 3 levels and gene expression, and M30 and 8-OHdG levels were determined. Target gene DNAs were propagated with the real time-PCR method. In the MTT studies, it was determined that the cell proliferation in rat renal epithelial cells (NRK-52E) treated with NaF+minerals was higher than that of all the NaF-treated groups and in the human osteoblast cells (hFOB 1.19), the cell proliferation was higher than in all the NaF-treated groups except for the MgCl2 group. The reason why the NaF administration in the NRK-52E cells resulted in an average of a 2-fold decrease in caspase 3 expression compared to the control group could be attributed to the apoptotic effect of NaF based on the time we obtained the RNA. However, based on this time, when the results are assessed based on the NaF and other mineral groups, the NaF-induced cytotoxic apoptosis might have used a pathway other than the apoptotic pathway. Thus, it is considered that minerals could usually prevent NaF-induced apoptosis by a synergistic mechanism due to the ionic character of NaF. NaF+mineral administration protected the NRK-52E cells from apoptosis. In the osteoblasts, on the other hand, it was concluded that NaF+mineral administration may be useful since it inhibits increased apoptosis.
Effects of Zinc Supplementation on Dna Damage in Rats With Experimental Kidney Deficiency
(Humana Press inc, 2017) Yegin, Sevim Ciftci; Dede, Semiha; Mis, Leyla; Yur, Fatmagul
This study was carried out to determine the effect of zinc on oxidative DNA damage in rats with experimental acute and chronic kidney deficiency. Six groups of five Wistar-Albino rats each were assigned as controls (C), acute kidney deficiency (AKD), zinc-supplemented (+Zn), acute kidney deficiency, zinc-supplemented (AKD + Zn), chronic kidney deficiency (CKD) and zinc-supplemented chronic kidney deficiency (CKD + Zn). The levels of 8-Oxo-2'-deoxyguanosine (8-OHdG) were determined, being the lowest in the CKD group (p < 0.05), higher in the C group than those of rats with CKD but lower than that of all the other groups (p < 0.05). There were no significant differences between the controls and the CKD + Zn group, or between the AKD and the +Zn groups. Among all groups, the highest 8-OHdG level was found in the AKD + Zn group (p < 0.05). DNA damage was greater in acute renal failure than in rats with chronic renal failure. The DNA damage in the zinc group was significantly higher than in the controls.
Effects of Α-Tocopherol on Serum Trace and Major Elements in Rats With Bleomycin-Induced Pulmonary Fibrosis
(Humana Press inc, 2006) Dede, Semiha; Mert, Handan; Mert, Nihat; Yur, Fatmagul; Ertekin, Ali; Deger, Yeter
The study was undertaken to investigate the influence of a-tocopherol on zinc, copper, iron, calcium, magnesium, and potassium concentrations in serum of rats with bleomycin-induced pulmonary fibrosis. Fourteen Wistar albino rats were randomly divided into two groups of seven animals each. The first group was treated intratracheally with bleomycin hydrochloride (BM group); the second group was also instilled with BM but received injections of alpha-tocopherol twice a week (BM+E group). The third group was treated in the same manner with saline solution only, acting as controls (C). The zinc concentrations of the BM and BM+E groups were significantly decreased compared to the controls (p < 0.05). The iron concentration of the controls was significantly higher than the other two groups. The magnesium concentration in the controls and the BM+E group was significantly higher than that of the BM group. The serum copper, calcium, and potassium concentrations were not found to be statistically different among the three groups. Distinct histopathologic changes were found in the BM group compared to the untreated rats. Less severe fibrotic lesions were also observed in the BM+E group. The results of this study show that lungs of rats treated with bleomycin were seriously damaged and that vitamin E seemed to counteract some of the damage, as indicated by differences in the serum concentrations of major elements.
Evaluation of Serum Lipoprotein and Tissue Antioxidant Levels in Sheep With Fluorosis
(int Soc Fluoride Research, 2013) Yur, Fatmagul; Mert, Nihat; Dede, Semiha; Deger, Yeter; Ertekin, Ali; Mert, Handan; Isik, Alper
The aim of this study was to evaluate serum lipoprotein and tissue antioxidant levels of sheep with and without fluorosis living in a volcanic area of Turkey. Fifteen Akkaraman sheep with fluorosis in the Agri region north of Lake Van and 10 Akkaraman sheep without fluorosis in the Van region just south of Lake Van in the eastern part of Turkey were investigated. In the kidney tissues, the MDA levels and SOD activities in the fluorosed sheep showed nonsignificant increases, but the GSH level and GPx activities significantly decreased. In the liver tissues of the fluorosed sheep, a significant increase in the MDA level and GPx activity was observed, but the GSH level showed no change, and the SOD activity exhibited a small decrease. By contrast, in the muscle tissues, the MDA level decreased and the SOD activity increased significantly, whereas the GPx activity increased but not significantly, and the GSH levels deoreased. Finally, the serum lipoprotein levels of the fluorosed and nonfluorosed sheep were not significantly different. In conclusion, different degrees in the pro-oxidant/antioxidant status of soft tissues such as kidney, liver, and muscle were affected by F intoxication, but no differences were found in the serum lipoprotein levels.
Glutatyon Uygulamasının Yüksek Glukoz Ortamında Oksidatif Dna Hasarı ve Antioksidan Sistem Üzerine Etkilerinin İn Vitro Olarak Değerlendirilmesi
(2022) Taspinar, Mehmet; Yur, Fatmagul; Dede, Semiha; Çetın, Sedat; Usta, Ayşe
Amaç: Bu çalışma, yüksek oranda glukoz ilave edilen BHK-21 hücre serisinde antioksidan özellikleri bilinen glutatyonun hücrelerde olası oksidatif DNA hasarı (8-hidroksi-2-deoksiguanozin) ve antioksidan sistem üzerine etkilerini ortaya koymak amacıyla planlandı. Gereç ve Yöntem: Bu amaçla, BHK-21 hücre serisinde in vitro koşullarda düzenli pasajları yapılarak (%5 FBS, %10 horse serum, %1 L-Glutamin, %1 penisilin/ streptomisin içeren RPMI 1640 besi yerinde ve %5 CO2 ve % 95 nem ve 37oC’de) inkübe edildi. MTT hücre canlılık testleri yapılarak glutatyonun kontrol grubuna göre ve glukozun IC50 değeri belirlendi. Hücreler pleytlere, 2x106 hücre olacak şeklide ekildi. Kontrol ve deneme grupları ve bu gruplar arasında çaprazlama olarak, çalışma grupları (glukoz; (285 mM), glutatyon (250 μM) hazırlandı. Yirmi dört saatlik inkübasyonu takiben tripsine edilen hücreler, dondur/çöz yöntemiyle parçalanarak analize hazırlandı. Elde edilen hücre kültürü lizatında; oksidatif DNA hasarı, TAS, TSO ve OSİ değerleri ELISA ile spektrofotometrik olarak ölçüldü. Bulgular: 8-OHdG düzeyleri, glukoz uygulanması ile önemli oranda arttığı, HG+GSH grubunda kontrole göre anlamlı olarak arttığı saptandı (p≤0.05). Sadece GSH verilen grup ise kontrolden farksız olarak bulundu. TAS bakımından, GSH uygulanan gruplarda kontrole fark bulunmazken, HG +GSH verilen grupda ise kontrole göre önemli artış tespit edildi (p≤0.05). TOS ve OSİ ise HG+GSH uygulanan gurupda kontrole göre önemli bir artış gözlendi (p≤0.05). Öneri: Çalışmadan elde edilen sonuçlara göre yüksek glukoz uygulanan hücrelere uygulanan glutatyonun hücresel düzeyde bu dozlarda koruyucu etkisi gözlenmemiştir. Ancak glutatyon uygulanan guruplarda, uygulanan glutatyon dozlarının hücreler üzerine toksik doz olmadığı da belirlenmiştir.
In Vitro Evaluation of Thymoquinone and Lycopene Supplementation on Oxidative Dna Damage and Oxidant Status in High Glucose Conditions
(Colegio Farmaceuticos Provincia de Buenos Aires, 2019) Dede, Semiha; Yur, Fatmagul; Taspinar, Mehmet; Cetin, Sedat; Usta, Ayse; Yuksek, Veysel
The present study was planned to investigate the effects of thymoquinone (TQ) and lycopene (LYC), known to possess pro-inflammatory and antioxidant properties, on oxidative DNA damage (8-hydroxy-2-cleoxyguanosine) in BHK-21 cell line treated with high glucose (FIG) and the antioxidant system. BHK-21 cell line was cultured with regular passages (5% FBS, 10% host serum, 1% L-glutamine, 1% penicillin/streptomycin - RPMI 1640, 5% CO2 and 95%, 37 degrees C incubation). MTT cell viability tests were conducted. Proliferative TQ and LYC and glucose IC50 values were determined. Control, study groups; glucose (285 mM), TQ (10 mu M), and LYC (50 mu M)) and cross groups were designed. After incubation, trypsinized cells were broken by the freeze/thaw method and analyzed. Oxidative DNA damage, TAS, TOS and OSI values were determined for the obtained samples. It was determined that 8-OHdG levels were affected by high glucose (p <= 0.05), they increased further with the administration of TQ and LYC in addition to HG. TOS and OSI values increased in all study groups when compared to the control (p <= 0.05), and TAS levels significantly decreased (p <= 0.05) with the administration of HG when compared to TQ and LYC groups. In conclusion, TQ and LYC administration in addition to high glucose exacerbated oxidative DNA damage and OSI, and decreased TAS when compared to TQ and LYC groups. The TQ and LYC dose and administration duration in addition to high glucose in the present study led to an improvement in oxidative balance in the BHK cell line.
Influence of Lycopene Administration on Neopterin, Myeloperoxidase and Gamma Glutamyl Transferase in Diabetic Rats
(Parlar Scientific Publications (p S P), 2018) Komuroglu, Ahmet Ufuk; Yur, Fatmagul; Ekin, Ismail Hakki
In this study, it was aimed to investigate the influence of lycopene administration on serum Neopterin (NEO), Myeleperoxidase (MPO) and Gamma glutamil tramsferase (GGT) activity in rats with experimentally induced diabetes. Male Wistar-Albino rats aged 7-8 weeks and weighing 250-300 gr were used in the study. Rats were randomly allocated to four groups as control, lycopene, diabetes and diabetes -lycopene with 7 rats in each. 45 mg/kg streptozotocin (STZ) prepared in cold citrate buffer was applied via intraperitoneal route in order to induce experimental diabetes. Lycopene was prepared in corn oil and administered via peroral route through gavage in the dose of 10 mg/kg daily in lycopene and DL groups. Blood samples were taken into serum tubes from the hearts of the rats under general anesthesia at the end of 28 days of test period. Blood samples were centrifuged and serum was obtained. Neopterin, MPO and GGT activities were determined in serum samples. The lowest neopterin level was detected in control group (p<0.001). The highest neopterin level was obtained in diabetes group, neopterin level of lycopene group was lower than that of diabetes group however a statistically significant difefrence was not detected. Neopterin level of DL group was found lower than that of diabetes and lycopene groups and this decrease was statistically significant (p<0.001). MPO level was found the lowest in diabetes group compared to other groups (p<0.001). MPO level of control group was found statistically significantly higher than that of lycopene and DL group (p<0.001). No statistically significant difference was observed between groups with regard to CRP levels. GGT activity was the highest in diabetes group and the lowest in DL group (p<0.001). In conclusion, inflammation markers, neopterin and GGT were low in the groups which received lycopene. These findings suggest that lycopene may be useful for prevention of the complications of diabetes and related inflammation.
Investigation of Blood Antioxidant Enzyme Levels and Glutathione Peroxidase, Catalase, and Superoxide Dismutase Gene Polymorphism in Sheep With Fluorosis
(int Soc Fluoride Research, 2017) Ozbey, Ulku; Deger, Yeter; Yur, Fatmagul; Cambay, Zafer; Ozbey, Gokben
Reactive oxygen species have been considered to play a significant part in the development of fluorosis, a critical public health issue in many parts of the world. OBJECTIVES: The aims of this study, conducted on two groups of sheep, with and without fluorosis, was (i) to determine the effect of fluorosis on antioxidant enzymes, such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and on malondialdehyde (MDA); and (ii) to investigate the relationship between the SOD, CAT, and GSH-Px genotypes and the antioxidant enzyme levels. METHODS: The two groups of sheep were: (i) 24 Akkaraman sheep with fluorosis living in Dogubeyazit province of Agri and (ii) 20 Akkaraman sheep with no signs of fluorosis living in Van. The MDA level and the antioxidant enzyme activities were identified spectrophometrically. The genotype characteristics were determined by the PCR-RFLP method using DNA extracted from blood. RESULTS: Compared to the control sheep without fluorosis, we found in the fluorotic sheep: (i) an increase in plasma MDA levels and GSH-Px-1 activity (p<0.05), and (ii) a decrease in SOD and CAT activities (p< 0.01). There were significant differences found in the genotype and allele frequencies in both groups (p<0.01). CONCLUSIONS: Fluoride-induced oxidative stress affected the membrane structure resulting in elevated erythrocyte MDA levels and reduced enzyme activity of the enzymes SOD and CAT. According to our data, the genepolymorphisms show a tendency towards fluorosis for our local sheep population.
Investigation of the Effect of Aminoglycosides on Angiotensin Converting Enzyme (Ace)
(2021) Yegin, Sevim Çiftçi; Değer, Yeter; Dede, Semiha; Yur, Fatmagul
Objective: The research attention to nephrotoxicity from antibiotics (as aminoglycosides), non-steroidal anti-inflammatory drugs, and antifungals, angiotensin-converting enzyme (ACE) inhibitors. Several drugs have resulted in produce an adverse effect on kidneys. Angiotensin-converting enzyme (ACE) is a component of the renin-angiotensin system (RAS), which leads to the conversion of angiotensin-I to angiotensin-II in vascular tissues. The present study aimed to determine serum ACE activity in rats with an aminoglycosine. Material-Method: In this study, two different groups formed, the control group (10 rats/Wistar-albino female) and the experimental group (30 rats/Wistar-albino female) administered aminoglycosine (Amikacin). Aminoglycosine was administered intraperitoneally to the experimental group at a single dose of 15 mg/kg body weight at the same time each day for 14 days. The control group implemented physiological water instead of aminoglycosine at the same rate. Result: When the groups compared according to the statistic results, it is observed that there was a significant increase in the experimental group according to the control group (p˂0.001). Conclusion: As a result, it was determined that aminoglycosin administered increased serum ACE activity and it was concluded that it may be useful to investigate the possibilities to evaluate it as a risk factor and indicator in the development of hypertension
Investigation of the Effects of Α-Tocopherol on the Levels of Fe, Cu, Zn, Mn, and Carbonic Anhydrase in Rats With Bleomycin-Induced Pulmonary Fibrosis
(Humana Press inc, 2007) Ertekin, Ali; Deger, Yeter; Mert, Handan; Mert, Nihat; Yur, Fatmagul; Dede, Semiha; Demir, Halit
This study was designed to examine the effects of vitamin E on the levels of Zn, Mn, Cu, Fe, and carbonic anhydrase in rats with bleomycin-induced pulmonary fibrosis. Twenty-one male Wistar albino rats were randomly divided into three groups: bleomycin alone, bleomycin+vitamin E, and saline alone (control group). The bleomycin group was given 7.5 mg/kg body weight (single dose) bleomycin hydrochloride intratracheally. The bleomycin+vitamin E group was also instilled with bleomycin hydrochloride but received injections of a-tocopherol twice a week. The control group was treated with saline alone. Animals were sacrified 14 d after intratracheal instillation of bleomycin. Tissue Zn, Mn, Cu, Fe, and carbonic anhydrase activities were measured in the lung and liver. Lung Cu, Fe, and carbonic anhydrase activity increase in both experimental groups. Zn and Mn levels decreased, except for the Mn level in the bleomycin group. Liver Zn, Mn, and Cu levels decreased in both experimental groups compared to the control group, whereas Fe and carbonic anhydrase activity increased in comparison to the control group. However, the liver tissue Fe level decreased compared to the control group. In the histopathologic assesment of lung sections in the bleomycin+vitamin E group, partial fibrotic lesions were observed, but the histopathologic changes were much less severe compared to the bleomycin-treated group.
Levels of Trace Elements in Muscle and Kidney Tissues of Sheep With Fluorosis
(Humana Press inc, 2016) Cetin, Sedat; Yur, Fatmagul
In this study, we report the concentrations of four trace elements in muscle and kidney tissues of sheep grown in an area with endemic fluorosis. Fifteen 3- to 4-year-old fluorotic sheep were selected for the study. Ten age-matched sheep with no sign of fluorosis were used as the control group. The animals were killed in a slaughterhouse in the village of DogubeyazA +/- t, located in the AgrA +/- province of Eastern Turkey, where kidney and muscle tissue samples were surgically obtained to be analyzed for copper, zinc, nickel, and iron. In muscle tissue of the fluorotic sheep, the copper levels were higher than those of the control group (p < 0.05). In the case of zinc, its levels were significantly higher in the controls than in the sheep with fluorosis (p < 0.01). No statistically significant differences were found in the muscle contents of nickel and zinc (p > 0.05). Compared to controls, the concentrations of zinc (0.01), iron (p < 0.05), and nickel (p < 0.05) were significantly higher in kidney tissues of fluorotic sheep, but there were no significant differences of the copper levels (p > 0.05). These results suggest that fluorosis significantly alters the mineral metabolism in muscle and kidney resulting in higher levels of mineral accumulation and excretion caused by fluoride intoxication. Further research shall focus on the enzymatic and metabolic activities of these and other trace elements.
Protective Effect of Α-Tocopherol on Oxidative Stress in Experimental Pulmonary Fibrosis in Rats
(Wiley, 2007) Deger, Yeter; Yur, Fatmagul; Ertekin, Ali; Mert, Nihat; Dede, Semiha; Mert, Handan
The study was undertaken to investigate the influence of alpha-tocopherol (vitamin E) on malondialdehyde (MDA) and glutathione (GSH) levels and catalase (CAT) activity in lung of rats with bleomycin-induced pulmonary fibrosis (PF). Fourteen Wistar-albino rats were randomly divided into two groups of seven animals each. The first group was treated intra-tracheally with bleomycin hydrochloride (BM group); the second group was also instilled with BM but received injections of alpha-tocopherol twice a week (BM + E group). The third group was treated in the same manner with saline solution only, acting as controls (C). There were decreases in GSH level and CAT activity while an increase in MDA level in BM group was found compared to the control group (p < 0.05). Vitamin E had a regulator effect on these parameters. After administration of (alpha-tocopherol, the increase in GSH level and CAT activity and the decrease in MDA level were seen in BM + E group compared to BM group (p < 0.05). Distinct histopathological changes were found in the BM group compared to the untreated rats. Less severe fibrotic lesions were also observed in the BM + E group. The results show that vitamin E is effective on the prevention of BM-induced PF, as indicated by differences in the lung levels of oxidants and antioxidants. Copyright (C) 2006 John Wiley & Sons, Ltd.