Dormancy Breaking Treatments for Wild Eremurus Spectabilis M.bieb Seeds

Abstract

Eremurus spectabilis M.Bieb seeds were subjected to moist-cold (4 degrees C) stratifications for different periods (0, 30, 40, 50, 60, 70, 80, 90, and 100 days), gibberellic acid (GA3) (0, 250, 500, 750, 1000, 1500, and 2000 ppm), potassium nitrate (KNO3) (0, 5 mM, 10mM, and 15mM), calcium chloride (CaCl2) (0, 5 mM, 10 mM, and 15 mM) treatments and in vitro germinations in different nutrient media (MS, B5, and WH) to break seed dormancy. Treated seeds were germinated at 15 degrees C under two different photoperiod regimes (continuous dark and 16/8 hours light/dark). The greatest rate of germination was obtained from a combination of 100-day moist-cold stratification + continuous dark photoperiod (73.30%). For mean germination rates, the 5 mM dose of KNO3 (10.00%) and CaCl2 (3.33%) treatments yielded better outcomes than the other doses. The same treatments were also more successful in emergence parameters. With regard to germination and emergence parameters, positive outcomes were not able to be achieved from in vitro germination treatments in different nutrient media and GA(3) treatments.