Van Yüzüncü Yıl Üniversitesi

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Effects of Irradiation Doses on Haploid Embryo and Plant Production in Naked and Shelled Seed Pumpkins
(International Society for Horticultural Science, 2012) Berber, M.; Yildiz, M.; Abak, K.
This study was carried out for two objectives: 1) to test efficiency of "pollination by irradiated pollen technique" in naked seed pumpkins (Cucurbita pepo var. styriaca) on parthenogenetic embryo stimulation and plant production compared to shelled seed genotypes (Cucurbita pepo var. pepo); and 2) to find the most suitable doses of irradiation both for naked and shelled seed pumpkins. As plant material, a total of 15 genotypes (ten naked and five shelled) were used and 3 irradiation doses of gamma rays (50, 100 and 150 Gy) were compared. During the study, a total of 2073 embryos were rescued from different genotypes and 979 of these developed into plants. Haploid embryos were obtained in all of the tested genotypes and no significant differences have been observed among genotypes. Although all of the three irradiation doses produced embryos and plants, more haploid plants were obtained from 150 Gy. 75 of the developed plants were acclimatized and cultivated in a polyethylene greenhouse and in 29 of these, ploidy levels were determined using indirect methods (existence of pollen, leaf and flower features, stoma density, chloroplast numbers in guard cells) and flow cytometry. As the result of the observations, 43% of these plants were found to be haploid and 57% were diploid. Haploid plant percentages were 36.7, 60.0 and 100.0% for 50, 100 and 150 Gy, respectively.
Efficacy of Issr and Srap Techniques for Molecular Characterization of Some Cucurbita Genotypes Including Naked (hull-Less) Seed Pumpkin
(Pakistan Agricultural Scientists Forum, 2012) Inan, N.; Yildiz, M.; Sensoy, S.; Kafkas, S.; Abak, K.
This study was carried out to determine the efficacy of inter simple sequence repeats (ISSR) and sequence-related amplified polymorphism (SRAP) techniques for molecular characterization some Cucurbita genotypes including naked (hull-less) seed pumpkin. Plant material consisted of sixteen genotypes belonging to Cucurbita pepo L., and four genotypes belonging to Cucurbita moschata Duchesne, and four genotypes belonging to Cucurbita maxima Duchesne. A larger part of the Cucurbita pepo genotypes (7 Turkish and 4 foreign) were seed pumpkin and some of them were naked seed types used as appetizer and/or for oil production. Morphological characterization was also done according to UPOV criteria. The sixty bands were obtained by using 8 ISSR primers and all these bands were found polymorphic. In the SRAP study, 8 primer combinations were used, a total of 71 bands were scored and all these bands were polymorphic. In the ISSR analyses, the genetic similarity coefficients varied between 0.07 and 0.96, while in SRAP it was between 0.13 1.0. The correlation coefficient between ISSR and SRAP genetic similarity data was very high (r = 0.947). The genetic variation as measured by ISSR and SRAP markers revealed high diversity among Cucurbita genotypes (H = 0.30, I = 0.46 and 100 % polym.). The genetic diversity among Turkish naked seed pumpkin genotypes (H = 0.09, I = 0.14 and 26.7 % polym.) was only a little less than that of the foreign accessions (H = 0.12, I = 0.18 and 32.1 % polym.). Some differences were observed between molecular and morphological studies, and it was concluded that only morphological or molecular analyses but a combination of both approaches are often more reliable in genetic variability studies in Cucurbita genus.
Genetic Diversity in Some Turkish Pepper (Capsicum Annuum L.) Genotypes Revealed by Aflp Analyses
(Academic Journals, 2009) Aktas, H.; Abak, K.; Sensoy, S.
The genetic relationships among 14 Turkish pepper (Capsicum annuum L) genotypes, 6 of them inbred lines, were determined by comparing their molecular traits. The taxonomic relationships and genetic variation among these genotypes were investigated with those of 5 foreign pepper genotypes. Fifty-six (26%) polymorphic AFLP markers out of total 215 DNA fragments from 4 primer pairs were used to define the genetic similarity among the pepper genotypes by dendrograms or two and three dimensional scaling. Two genotype-specific markers for the genotype PM-702 were among the polymorphic ones. The inbred lines of Alata Agricultural Research Institution were partitioned to similar clusters and constituted extremely low genetic variation. On the other hand, other local Turkish genotypes had comparatively higher genetic diversity.
Response of Turkish Melon Genotypes To Fusarium Oxysporum F. Sp Melonis Race 1 Determined by Inoculation Tests and Rapd Markers
(Eugen Ulmer Gmbh Co, 2007) Sensoy, S.; Demir, S.; Buyukalaca, S.; Abak, K.
The response to Fusarium oxysporum f. sp. melonis (F.o.m.) race 1 of 79 Cucumis melo L. genotypes - 56 accessions collected from different parts of Turkey, 5 local cultivars and 18 foreign genotypes - was determined by using pathogenicity tests and RAPD markers (E07 and G17). For pathogenicity tests, at least 15 individuals of each melon genotype were artificially inoculated by either conidia suspension culture or sand culture of F.o.m. race 1. Melon seedlings were evaluated on a 0-3 scale for the presence of vascular browning two weeks after inoculation with conidia suspension culture resp. four weeks after inoculation with sand culture. The disease incidence on the 79 melon genotypes ranged from 0 to 100%. Seven melon genotypes were resistant (CU129, CU258, T8, Y9, Y10, Y15, and Y63), three melon genotypes (CU280, CU309, and T1) were heterogeneous, and all the others were susceptible to F.o.m. race 1 based on disease incidence and vascular browning. The success of the E07 marker in detecting susceptible genotypes was higher than that of G17; the mistmatch ratios of E07 and G17 markers were 5.06 and 58.23%, respectively.

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