Browsing by Author "İlhan, M."

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Assessment of Antidepressant Effect of the Aerial Parts of Micromeria Myrtifolia Boiss. & Hohen on Mice
(NLM (Medline), 2019) Akkol, E.; Gürağaç Dereli, F.T.; İlhan, M.
The currently available antidepressant agents necessitate the development of newer alternatives because of their serious adverse effects and costs. Traditional medicinal knowledge is likely the key that opens the door to discover new medicines. In Turkish folk medicine, the infusion prepared from aerial parts of Micromeria myrtifolia Boiss. & Hohen is used as pleasure and medicinal tea for its relaxing action. The present research was conceived to confirm the antidepressant's potential of this traditional medicinal plant. In this process, first of all, the collected and shade-dried aerial parts of M. myrtifolia were powdered and then, extracted using solvents with different polarity as follows; n-hexane, ethyl acetate (EtOAc), and methanol (MeOH). The antidepressant activity of the extracts was evaluated by using several in vivo and in vitro experimental models of depression. When the data obtained from the control and experimental groups were compared, it was determined that the MeOH extract was the most active. The active components of this extract were isolated and identified utilizing various chromatographic separation techniques. The MeOH extract was applied to reversed phase (RP-18) column chromatography to obtain five main fractions and they were tested on antidepressant activity models. The isolated compounds from the obtained fractions were elucidated as rosmarinic acid (1), myricetin (2), apigenin (3), and naringenin (4) which were assumed to be responsible for the antidepressant activity of the aerial parts. According to the results, rosmarinic acid, myricetin, apigenin, and naringenin showed statistically significant activity on forced swimming test and tetrabenazine-induced ptosis models, whereas only rosmarinic acid showed statistically significant activity on the tail suspension test. Apigenin displayed the highest inhibitory activity on MAO A and B enzymes. Studies in the future should be performed to investigate the antidepressant activity mechanism of these natural compounds. The current research could be an important step in the development of the new agents that can be used in the treatment of depression. This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine
Leaf Anatomy of Inula Peacockiana (Aitch. & Hemsl.) Korovin
(University of Ankara, 2022) İlhan, M.; Hürkul, M.M.
Objective: Inula peacockiana, which is called "uzunandızotu" in Turkey, is a stout perennial herbaceous plant with yellow flowers, grow up to 2 m. It is a plant belonging to the Asteraceae family, which has medicinal value. The safe use of medicinal plants depends on their correct diagnosis. The anatomical features of the leaves are valuable in diagnosing plants correctly. In this study, the anatomical features of the basal leaf, petiole and cauline leaf of I. peacockiana were investigated. Material and Method: Plant materials were collected from Van (Turkey). A voucher specimen was deposited in the VANF Herbarium. The samples for anatomical studies were preserved in 70% alcohol. The transverse and surface sections were cutted by hand with razor blade in microscopic preparation form. The chloral hydrate solution was used in microscopic examinations. Result and Discussion: The basal and cauline leaves are dorsiventral and contain cover and glandular hairs. The main vein protrudes outward in the midrib of the both leaves. Different the basal leaf, the cauline leaf contains more glandular hairs on the upper surface and cover hairs on the lower surface. In both leaves, ranunculaceae type stomata were observed in the upper and lower epidermis. The simple arc-shaped petiole includes of numerous separate bundles embedded in the parenchymatous cells. The epidermal layer consist of numerous cover and glandular hairs. © 2022 University of Ankara. All rights reserved.