Browsing by Author "Abd El-Aty, A. M."

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A Combination of Omega-3 and Exercise Reduces Experimental Achilles Tendinopathy Induced With a Type-1 Collagenase in Rats
(Canadian Science Publishing, 2023) Gundogdu, Gulsah; Tasci, Seymanur Yilmaz; Gundogdu, Koksal; Kapakin, Kubra Asena Terim; Demirkaya, Alper Kursat; Nalci, Kemal Alp; Abd El-Aty, A. M.
This study aimed to evaluate the effectiveness of omega-3 supplementation with exercise in a collagenase-induced Achilles tendinopathy (AT) rat model. Experimental groups (healthy control (HC), AT, exercise (Ex), omega-3 (W), and Ex+W) were randomly allocated. After a week of adaptation, oral omega-3 was initiated for 8weeks (5 days/week). The exercise groups performed treadmill running for 30min/day (5 days/week, 20m/min, 8 weeks) following one week of adaptation (10 m/min, 15 min/day). Matrix metalloproteinase-13 (MMP-13), interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), and total antioxidant-oxidant status (TAS) levels were determined in serum samples. Tendon samples were obtained for biomechanical, histopathological, and immunohistochemical assessments. Ultimate tensile force, yield force, stiffness values, collagen type-I alpha 1 expression, and serum TAS significantly decreased (P < 0.05) in AT vs. HC. These values and expression significantly increased in the Ex+W group vs. AT. Serum MMP-13, IL-1 beta, and TNF-alpha levels decreased in all treatment groups vs. AT. The most significant decrease was found in the Ex+W group (P < 0.01). Histopathologically, the improvement in degeneration was statistically significant in the Ex+Wgroup (P < 0.05). Immunohistochemically, MMP-13, IL-1 beta, TNF-alpha, and nitric oxide synthase2 expression was decreased in all treatment groups vs. AT. In conclusion, omega-3 and exercise might be recommended in AT patients.
Effect of Metformin/Irinotecan-loaded Poly-Lactic Acid Nanoparticles on Glioblastoma: in Vitro and in Vivo Studies
(Future Medicine Ltd, 2018) Taghizadehghalehjoughi, Ali; Hacimuftuoglu, Ahmet; Cetin, Meltem; Ugur, Afife Busra; Galateanu, Bianca; Mezhuev, Yaroslav; Abd El-Aty, A. M.
Aim: The present study was designed to evaluate the effects of irinotecan hydrochloride (IRI)- or metformin hydrochloride (MET)-loaded poly-lactic-co-glycolic acid (PLGA) nanoparticles (NPs) for the treatment of glioblastoma multiforme using in vitro neuron and U-87 MG glioblastoma cell cultures and in vivo animal model. Methods: The cytotoxic and neurotoxic effects of pure drugs, blank NPs and MET- and IRI-loaded PLGA NPs were investigated in vitro (using methylthiazolyldiphenyl-tetrazolium bromide assay) and in vivo (using Cavalieri's principle for estimation of cancer volume). Results: 1 and 2 mM doses of MET and MET-loaded PLGA NPs, respectively, significantly reduced the volume of extracted cancer. Conclusion: Consequently, MET- and IRI-loaded PLGA NPs may be a promising approach for the treatment of glioblastoma multiforme.