Browsing by Author "Akar, K."

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Knowledge, Attitudes, and Practices (KAP) Relating to Brucellosis: Unveiling the Vital Role of Public Awareness
(Elsevier, 2025) Akar, K.
Brucellosis is a neglected, complex, and difficult-to-diagnose zoonotic disease caused by Brucella spp. It is a highly pathogenic and highly prevalent disease worldwide. National eradication policies need to be developed to reduce the negative impact of this disease on public health and the economy, and to control the disease. Therefore, the disease's prevalence, distribution, and economic impact should be investigated comprehensively. In many societies around the world, knowledge, attitude, and practice studies (KAP) are used to measure the level of knowledge about pathogenic infections and their risks and to determine the most appropriate strategy to implement. For this purpose, reference laboratories in the countries where the disease is observed have a significant role in diagnosing the disease. It is known that the incidence of the disease can be reduced by the efforts of these institutions to increase the level of knowledge in society, together with the diagnosis of the disease. Biosecurity measures should be taken in doing so, starting with people at high risk of occupational exposure. It is crucial to create public health awareness about brucellosis. Therefore, it is thought that it can be used in determining an effective eradication strategy for the prevention of the disease by evaluating the KAP of shepherds with the help of questionnaires and determining the risk factors related to the disease. This study evaluated the importance of brucellosis in public awareness globally and the differences or diversity in the KAP of communities regarding animal brucellosis. © 2026 Elsevier Inc. All rights reserved.
Microbial Contamination of in Vitro-Derived Cattle Embryos and Resistance Genes
(Sciendo, 2025) Sanioglu Golen, G.S.; Akar, K.; Karasahin, T.; Şentürk, G.; Gölen, S.; Aras, Z.
The global trend of in vitro embryo systems, particularly the in vitro fertilization (IVF) culture system, is gaining momentum. Despite the strict standards followed in in-vitro embryo procedures, microbiological contamination is occasional, and the relevant literature is scarce. In this study, for the first time, IVF culture dishes with microbial contamination and resistance genes of isolates were evaluated in veterinary medicine. Samples were microscopically taken from IVF tissue cultures suspected of bacterial or fungal contamination and sent to the microbiology laboratory for further examination. The total contamination rate was 11.1% in IVF cultures where cell division did has stopped or turbidity occurred. Identification of contaminant microorganisms showed that infections were mainly caused by E. coli 9.5% and Candida spp. 1.58%. A set containing multiplex antibiotic primers was used during the IVF protocol to determine antibiotic resistance genes. All E. coli isolates were resistant to penicillin used in the Kirby-Bauer, and 16% was resistant to streptomycin. This study is the first systematic evaluation of microbial contamination of bovine IVF culture vessels in veterinary medicine. IVF culture should be evaluated in more detail to learn more about the source of the microorganism and to develop adequate measures to prevent microbial contamination. © 2025 Gokcenur Sanioglu Golen et al., published by Sciendo.