Browsing by Author "Alkan, M"

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The Effect of Nigella Sativa (Black Seed) and Vit. E Plus Selenium in the Prevention of Liver Necrosis Experimentally Induced With Carbon Tetrachloride (Ccl4) in Rats
(Tubitak Scientific & Technological Research Council Turkey, 2003) Sahin, A; Yener, Z; Dagoglu, G; Dede, S; Oto, G; Alkan, M
The preventive effect of Nigella sativa (black seed) was investigated in rats with hepatic necrosis experimentally induced by carbon tetrachloride (CCl4). To compare the antioxidant effect of black seed, well-known antioxidants, vitamin E and selenium mineral, were used. This study was conducted on 40 rats that were divided into five groups of eight. Group A was separated as a control group and treated intraperitoneally (i.p.) with only liquid paraffin. Group B was administered i.p CCl4 in liquid paraffin. Group C was administered i.p. CCl4 in liquid paraffin and intramuscularly (i.m.) treated with vitamin E + selenium. Group D was administered i.p. CCl4 in liquid paraffin, i.m. treated with vitamin E + Se and fed ad libitum with standard rat pellets containing 10% black seed. Group E was administered i.p. CCl4 in liquid paraffin and fed ad libitum with standard rat pellets containing 10% black seed. Blood samples were drawn from the heart of the rats four times for the experimental groups and five times for the control group, one of which was at the beginning of the experiment. In blood samples, malondialdehyde (MDA), glutathione peroxidase (GSH-px), superoxide dismutase (SOD), alanine transaminase (ALT), aspartic transaminase (AST), intracellular glutathione (GSH) and ceruloplasmin analyses were performed. In group B blood samples, lipid peroxidation and antioxidant enzymes were significantly increased. The closest enzyme activities to those of the control group were found in group E. The least weight loss percentages were in group E. The least necrosis was detected in the liver samples of group D. Histopathological results were consistent with biochemical results. In conclusion, a liver necrosis preventive effect of vitamin E + selenium and black seed was observed.
Oxidation Products of Nitric Oxide and the Concentrations of Antioxidant Vitamins in Parasitized Goats
(Vysoka Skola veterinarni Farmaceuticka, 2002) Dede, S; Deger, Y; Kahraman, T; Deger, S; Alkan, M; Cemek, M
The status of nitric oxide oxidation products and antioxidant vitamins were investigated in goats infected with endoparasites and blood parasites (Trichostrongylidae sp. + Protostrongylidae sp. + Eimeria sp. + Babesia sp.), in this study. Eighteen goats were naturally infected with these parasites and ten healthy goats served as controls which had been treated with antiparasitic drugs after parasitological examinations were carried out. The concentrations of nitric oxide oxidation products (nitrate, nitrite) and antioxidant vitamins (vitamins E And C, beta-carotene and retinol) were determined spectrophotometrically in the blood serum of all Pats. The results were expressed as nitrate (mug/ml) 7.25 +/- 1.31-4.69 +/- 0.32; nitrite (mug/ml) 1.52 +/- 0.39-1.64 +/- 0.19; vitamin E (mg/100) 0.13 +/- 0.05-0.42 +/- 0.02; vitamin C (mg/100 ml) 1.49 +/- 0.26-1.46 +/- 0.15; retinol (g/100 ml) 201.51 +/- 15.69-234.081 +/- 45.15; beta-carotene (g/100 ml) 62.71 +/- 7.14-53.95 +/- 3.82. In conclusion, nitrate concentrations of the infected group were higher than controls (p < 0.05) whereas vitamin E levels of the infected group were lower than the control group (p < 0.05). The concentrations of the other indices examined were not statistically different between groups. These results suggest that the parasitic infections have direct effects on the concentrations of vitamin E, an important antioxidant, and on the increase of nitrate levels which may result from the pathophysiological effects of the parasitic infections.
Studies on the Effects of X-Ray on Erythrocyte Zinc and Copper Concentrations in Rabbits After Treatment With Antioxidants
(Humana Press inc, 2003) Dede, S; Deger, Y; Mert, N; Kahraman, T; Alkan, M; Keles, I
The aim of this study was to investigate the effect of supplemental antioxidant vitamins and minerals on the erythrocyte concentrations of zinc and copper in rabbits after exposure to X-rays. The animals were divided into two experimental and one control group (CG). The first group (VG) was given daily oral doses of vitamins E and C; supplemental amounts of manganese, zinc, and copper were mixed with the feed and given to the second group of experimental animals (MG). Blood samples were taken from all groups before and after 4 wk of vitamin and mineral administration and after irradiation with a total dose of 550-rad X-rays. The administration of minerals caused the most significant increases of Zn and Cu. Even after irradiation, the zinc levels in the irradiated animals were higher than in the nonirradiated vitamin-supplemented animals (p<0.05). The results suggest that supplementation with antioxidant vitamins and minerals may have a protective effect against X-ray-induced damage.