Browsing by Author "Alp, Hamit H."

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The Comparison of Resveratrol and N-Acetylcysteine on the Oxidative Kidney Damage Caused by High Dose Paracetamol
(Colegio Farmaceuticos Provincia de Buenos Aires, 2015) Yapanoglu, Turgut; Adanur, Senol; Ziypak, Tevfik; Arslan, Aynur; Kunak, Celalettin S.; Alp, Hamit H.; Suleyman, Bahadir
In this study, the effects of resveratrol were investigated on paracetamol-induced oxidative kidney damage in rats and compared with N-acetylcysteine (NAC). Rats were divided into healthy (HG), paracetamol control (PAC), resveratrol + paracetamol (RPA), NAC + paracetamol (NPA) groups. RPA group was administered 30 mg/kg resveratrol, NPA group was administered NAC in 300 mg/kg doses. Distilled water was administered to HG and PAC group. One hour after administration of the drugs and distilled water, all groups (except for HG) were given paracetamol 1000 mg/kg dose. 24 h after paracetamol administration, animals were killed by anesthesia and their kidneys were removed. Biochemical investigations were carried out on the kidneys. Resveratrol and NAC almost equally suppressed the increase of malondialdehyde (MDA) amount in the renal tissue of paracetamol-administered group. But resveratrol reduced the increase of 8-hydroxyguanine (8-OHGua), creatinine and blood urea nitrogen (BUN) more significantly than NAC. Resveratrol prevented loss of total glutathione (tGSH) in rats taking paracetamol better than NAC. As a result, resveratrol was determined to prevent oxidative renal damage and functional disorder related to paracetamol better than NAC.
The Effect of Pde5 Inhibitors on Bone and Oxidative Damage in Ovariectomy-Induced Osteoporosis
(Sage Publications Ltd, 2017) Alp, Hamit H.; Huyut, Zubeyir; Yildirim, Serkan; Basbugan, Yildiray; Ediz, Levent; Sekeroglu, Mehmet R.
Osteoporosis is a major public health problem associated with many factors, and it affects more than 50% of women over 50 years old. In the current study, our purpose was to investigate the effects of phosphodiestarase-5 inhibitors on osteoporosis via the nitric oxide/3',5'-cyclic guanosine monophosphate/protein kinase G signalling pathway. A total of 50 female albino Wistar rats were separated into five groups. The first group was appointed as the healthy control group with no ovariectomy. All animals in the other groups underwent a bilateral ovariectomy. Six months after the ovariectomy, vardenafil, udenafil and tadalafil were given to the third, fourth and fifth groups, respectively, but were not administered to the positive control group (10 mg/kg per day for two months). The bone mineral density values were determined using a densitometry apparatus for all groups pre- and post-ovariectomy as well as after treatment. The levels of nitric oxide, endothelial nitric oxidesynthase, asymmetric dimethylarginine, 3',5'-cyclic guanosine monophosphate, protein kinase G, phosphodiestarase-5, pyridinoline, deoxypyridinoline, carboxyterminal telopeptide fragments and plasma carboxy terminal propeptide of type I collagen were determined using an enzyme linked immunosorbent assay. The levels of malondialdehyde, 8-hydroxy-2-deoxy guanosine, deoxyguanosine and coenzyme Q10 were determined by a high-performance liquid chromatography assay. Additionally, the right femoral trabecular bone density and the epiphyseal plate were measured in all groups. Angiogenesis was histologically observed in the bone tissue. In addition, we determined that the inhibitors may have caused a positive impact on the increased bone mass density and reduction of bone resorption markers. We also observed the positive effects of these inhibitors on oxidative stress. In conclusion, these phosphodiestarase-5 inhibitors increase angiogenesis in bone tissue and improve the re-formation rate of bone in rats with osteoporosis. Chemical compounds studied in this article Udenafil (PubChem CID: 6918523); Tadalafil (PubChem CID: 110635); Vardanafil (PubCham CID: 110634). Impact statement The results in our study appear to establish the osteoporosis model and provide evidence of the positive effects of three separate PDE5 inhibitors (vardenafil, udenafil, and tadalafil). The positive effects of these PDE5 inhibitors are investigated and demonstrated by the bone mass density and bone resorption markers. These effects are associated with significant demonstrated antioxidant activities. Osteoporosis is a significant major public health problem especially in more aged populations. Advances in identifying and understanding new potential therapeutic modalities for this disease are significant. This study provides such an advance.
Evaluation of Oxidative Damage and Antioxidant Mechanisms in Copd, Lung Cancer, and Obstructive Sleep Apnea Syndrome
(Daedalus Enterprises inc, 2016) Sunnetcioglu, Aysel; Alp, Hamit H.; Sertogullarindan, Bunyamin; Balaharoglu, Ragip; Gunbatar, Hulya
BACKGROUND: Oxidative damage is a major contributing factor to carcinogenesis and obstructive disorders in lungs. Current evidence suggests that the inflammatory processes yield to oxidative mechanisms, which underlie COPD, lung cancer, and obstructive sleep apnea syndrome (OSAS). This study aimed to evaluate the oxidative damage in these diseases by evaluating the oxidative and antioxidant biomarkers. METHODS: Malondialdehyde, 8-oxo-7,8-dihydro-2'-deoxyguanosine, and coenzyme Q10 levels were evaluated in the blood samples of subjects with COPD, lung cancer, and OSAS by high-pressure liquid chromatography. RESULTS: A total of 111 participants (35 females, 76 males) with OSAS (n = 29), COPD (n = 26), and lung cancer (n = 28) and healthy controls (n = 28) were included in the study. The malondialdehyde and coenzyme Q10 levels were significantly higher in all 3 diseases when compared with controls (P < .01), whereas 8-oxo-7,8-dihydro-2'-deoxyguanosine levels were only significantly higher than in healthy controls in subjects with lung cancer (P = .005). The highest levels of malondialdehyde and coenzyme Q10 were determined in subjects with OSAS and lung cancer, respectively. The highest 8-oxo-7,8-dihydro-2'-deoxyguanosine levels were also observed in subjects with lung cancer, but the differences of this biomarker with other diagnoses were not statistically significant (P = .56). CONCLUSION: Oxidative damage was observed in all 3 diagnoses, and, as a response to oxidative stress, antioxidant mechanisms were also active in these diseases. Malondialdehyde and 8-oxo-7,8-dihydro-2'-deoxyguanosine were found to be efficiently usable in the evaluation of oxidative damage in chronic respiratory diseases.
Investigation of Antiulcer and Antioxidant Activity of Moclobemide in Rats
(Aves, 2015) Albayrak, Abdulmecit; Alp, Hamit H.; Suleyman, Halis
Objective: Even though there are many drugs for the treatment of gastric ulcers, these drugs sometimes cannot succeed. Since the 1950s, antidepressant drugs have been used for several non-psychiatric indications. Many antidepressant drugs have been shown experimentally to produce antiulcer activity in various ulcer models. Moclobemide is an antidepressant drug which inhibits monoamine oxidase-A (MAO) enzyme selectively. When it is compared to the classic antidepressants drugs, moclobemide is the first choice in depression treatment because of its effectiveness and less side effects. This study aimed to investigate the antiulcer effects of moclobemide and to determine its relationship with antioxidant mechanisms in rat gastric tissue. Materials and Methods: The antiulcer activities of 10, 20, 40, 80, 150 mg/kg moclobemide and 20 mg/kg famotidine have been investigated on indomethacin-induced ulcers in rats, and the results have been compared with that of the control group. Results: Moclobemide decreased the indomethacin-induced ulcers significantly at all doses used. While used doses of moclobemide increased the glutathione (GSH), nitric oxide (NO) level and superoxide dismutase (SOD) activity, it decreased the malondialdehyde (MDA) level and myeloperoxidase (MPO) activity in stomach tissue when compared to the control group. Conclusion: It is determined that an antidepressant drug, moclobemide is a potent anti-ulcer agent. Inhibition of toxic oxidant radicals and activation of antioxidant mechanisms play a role in its antiulcer effect mechanisms.
Monomer Release From Bulk-Fill Composite Resins in Different Curing Protocols
(Nihon Univ, School dentistry, 2020) Gul, Pinar; Alp, Hamit H.; Ozcan, Mutlu
The purpose of this study was to determine the depth of cure and the type and amount of monomers released from bulk-fill composites in different curing protocols. Five different composite resins Filtek Bulk-Fill Posterior, Filtek Bulk-Fill Flowable, SureFil SDR, X-tra Fil, and X-tra base. were used. A light-emitting diode (LED) device was used in 3 different modes (standard, high power, and extra power mode), and a halogen light device was also used as a control. Surface hardness was measured and the depth of cure was calculated. Monomer analysis was performed using high performance liquid chromatography (HPLC). The data were analyzed using Tamhane's T2 post-hoc test (alpha = 0.05). The cure depth for all materials except for Filtek Bulk-Fill Posterior (extra power mode) and Filtek Bulk-Fill Flowable (high power and extra power modes) was over 80%. Under the conditions of this study, the amount of monomer released from composite resins changed according to the type of composite resin and the light mode used.
Monomer Release From Nanohybrid Composites After Bleaching
(Nihon Univ, School dentistry, 2019) Gul, Pinar; Karatas, Ozcan; Alp, Hamit H.; Cam, Ihsan B.; Ozakar-Ilday, Nurcan
The evaluation of the effect of bleaching on monomer release from two composite resins was performed by bleaching two nanohybrid composite resins Filtek Z550 and Tetric N-Ceram using two bleaching products Whiteness HP Maxx and Vivastyle. In total, 20 samples were made from each composite resin from w hich four groups were fabricated (two for each bleaching product). The samples were stored in a 75% ethanol solution, and the solutions were renewed after 1, 7, and 28 days. The monomer release IN as analyzed using high performance liquid chromatography. The data were analyzed using repeated measures analysis of variance and least significant difference multiple comparison test (alpha = 0.05). Monomers were found to be released from both composite resins. The amounts of monomer released were found to decrease over time (P < 0.05); however, the resins were not affected by bleaching, and the released monomers were well below toxic doses.
Neuroprotective Effects of Thymoquinone on the Hippocampus in a Rat Model of Traumatic Brain Injury
(Elsevier Science inc, 2016) Gulsen, Ismail; Ak, Hakan; Colcimen, Nese; Alp, Hamit H.; Akyol, Mehmet E.; Demir, Ismail; Ragbetli, Murat C.
BACKGROUND: Traumatic brain injury is a leading cause of morbidity and mortality worldwide. We evaluated the neuroprotective effects of thymoquinone (TQ) in a rat model of traumatic brain injury by using biochemical and histopathologic methods for the first time. MATERIALS AND METHODS: Twenty-four rats were divided into sham (n = 8), trauma (n = 8), and TQ-treated (n = 8) groups. A moderate degree of head trauma was induced with the use of Feeney's falling weight technique, and TQ (5 mg/kg/day) was administered to the TQ-treated group for 7 days. All animals were killed after cardiac perfusion. Brain tissues were extracted immediately after perfusion without damaging the tissues. Biochemical procedures were performed with the serum, and a histopathologic evaluation was performed on the brain tissues. Biochemical experiments included malondialdehyde (MDA), reduced and oxidized coenzyme Q10 analysis, DNA isolation and hydroylazation, and glutathione peroxidase, and superoxide dismutase analyses. RESULTS: Neuron density in contralateral hippocampal regions (CA1, CA2-3, and CA4) 7 days after the trauma decreased significantly in the trauma and TQ-treated groups, compared with that in the control group. Neuron densities in contralateral hippocampal regions (CA1, CA2-3, and CA4) were greater in the TQ-treated group than in the trauma group. TQ did not increase superoxide dismutase or glutathione peroxidase antioxidant levels. However, TQ decreased the MDA levels. CONCLUSIONS: These results indicate that TQ has a healing effect on neural cells after head injury and this effect is mediated by decreasing MDA levels in the nuclei and mitochondrial membrane of neurons.