Browsing by Author "Arabaci, Muhammed"

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Comparison of Rainbow Trout (Oncorhynchus Mykiss) and Brown Trout (Salmo Trutta Ssp) Dual Culture in Different Ratios and Growth Performances
(Zoological Soc Pakistan, 2017) Karatas, Boran; Arabaci, Muhammed; Onalan, Sukru
The objective of the present study is to analyze the growth performance of rainbow trout (Oncorhynchus mykiss) (G) and brown trout (Salmo trutta ssp) (K) monoculture and polyculture in 75-25%, 66-34% and 50-50% stocking rates and its effects on food utilization and to determine the results with respect to aquaculture. Stocking was conducted in 5 different ratios in the study and each different stocking ratio was considered as a group and 24 fish were used in each group. Study groups were named based on stocking ratio percentages as G, K, G75K25, G66K34 and G50K50 and the study lasted 80 days. When the growth parameters in polycultures of rainbow trout and brown trout are considered, it was determined that the growth rates were not statistically different between rainbow trout monoculture and rainbow trout G75K25 and G50K50 groups, however rainbow trout in G66K34 experienced statistically significantly less growth when compared to others (p<0.05). Growth parameters of brown trout in polycultures demonstrated that the best growth was observed in G66K34 polyculture group (p<0.05). Study results showed that there was no significant difference between the groups based on food conversion coefficients and survival rates (p>0.05). As a result, it was determined that brown trout monoculture was the most advantegous group followed by G50K50 polyculture group, and when ecological and environmental parameters are concerned, G75K25 group, which consumed statistically significantly less food and demonstrated a lower food conversion ratio, would be more beneficial, and for fish breeders, who would like to increase the growth rate of the trout, G66K34 polyculture group would be more useful when compared to brown trout monoculture.
Determination of Bacterial Disease Map for Rainbow Trout Farms in Van Province
(Amer inst Physics, 2016) Arabaci, Muhammed; Onalan, Sukru
Lactococcosis, yersiniosis, listenollosis and cold water disease agents are frequently observed in Turkey as bacterial fish pathogens. Bacterial fish pathogens have high mortality prognosis, causing significant economic losses for the businesses. Use of molecular methods in substantiation of disease factors became prevalent in recent years. These methods have a significant role in fast diagnosis and early treatment of fish diseases. In the present study, 8 rainbow trout samples were obtained from each of 19 rainbow trout farms located in Van province, Turkey and registered with Food, Agriculture and Livestock Ministry. Total genomic DNAs were isolated from kidney tissues of sampled rainbow trout. Obtained DNAs were analyzed with real-time PCR there is/not (+/-) analysis using disease specific primer pairs for each disease. Molecular diagnosis of lactococcosis pathogen in 4 farms out of 19 rainbow trout farms active in Van province, and yersiniosis pathogen in 1 farm were made as a result real-time PCR analysis. Listenollosis and cold water pathogens were not molecularly observed. Results of the present study demonstrated that the region was safe for bacterial fish pathogens of cold water disease and listenollosis, which are observed frequently in Turkey, and there were deficiencies in preventive measures against lactococcosis and yersiniosis and fish transfer was a significant reason for the prevalence these diseases.
Effects of Different Led Light Spectra on Rainbow Trout (Oncorhynchus Mykiss): in Vivo Evaluation of the Antioxidant Status
(Springer, 2020) Guller, Ugur; Onalan, Sukru; Arabaci, Muhammed; Karatas, Boran; Yasar, Muhterem; Kufrevioglu, Omer Irfan
Rainbow trout (Oncorhynchus mykiss) farming is one of the major aquacultures in Turkey. Some conditions in fish farming can induce oxidative stress leading to the deterioration in properties such as appearance/color, texture, and flavor in fish meat. This situation may cause the consumer not to prefer edible fish. Although there are some studies on the impacts of light intensity on fish welfare, the changes in the antioxidant enzyme activities have not been elucidated. In the current study, it was intended to examine in rainbow trout how cultivating under different wavelengths affects the antioxidant enzymes and acetylcholine esterase (AChE) activity, because its activity is associated with oxidative stress, and also the determination of which light is suitable for fish welfare was aimed. Rainbow trout larvae were grown under four lights with different wavelengths: natural sunlight and incandescent long-wave (red light), medium-wave (green light), and short-wave (blue light) LED light. The experiment lasted for 64 days. Biochemical assays were carried on in the brain, gill, and liver of rainbow trout. Antioxidant enzymes and AChE activity, which play an important role in the central nervous system, were assayed. In gill tissues, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glucose 6 phosphate dehydrogenase (G6PD), glutathione reductase (GR), glutathione S-transferase (GST), and AChE activities increased under all three light wavelengths. In the liver, while activities of antioxidant enzymes and AChE decreased in red light, all of them increased in blue and green light. In the brain, GPx, GST, G6PD, and SOD activities were reduced but AChE activity did not alter under all three light sources. In conclusion, light sources with different spectral structures caused important changes in the activities of antioxidant enzymes in rainbow trout. On this basis, it may be thought that this may be a response to the changing redox status of a cell. Based on our results, blue light sources may be suggested for fish welfare in rainbow trout culture, and providing fish welfare by changing light sources can be easy and cheap in fish farming.
The Effects of Light Sources With Different Spectral Structures on Ocular Axial Length in Rainbow Trout (Oncorhynchus Mykiss)
(Academic Press Ltd- Elsevier Science Ltd, 2016) Timucin, Ozgur Bulent; Arabaci, Muhammed; Cuce, Ferhat; Karatas, Boran; Onalan, Sukru; Yasar, Muhterem; Karadag, M. Fatih
Every day, we are acquiring more and more clues regarding the effects of different spectral structures (SS) of light on ocular axial length (OAL). As a step towards understanding this association, this study sought to characterise the effects of light sources of different SS on OAL in fish through comparisons with indoor daylight. The experimental design was completely randomised with 4 treatments and 2 replications. Three hundred and fifty two rainbow trout (Oncorhynchus mykiss) were housed in 8 tanks and fed for 71 days. Differences in the mean values of ocular elongation were determined at the end of the experiment. The daylight group was exposed to indoor daylight in the hatchery environment, the red group was exposed to long wavelength light (600-650 nm), the green group was exposed to mid wavelength light (495-570 nm) and the blue group was exposed to short wavelength light (420-495 nm). The values of the OALs in fish grown under the same light intensity, but with light of different spectral characteristics, demonstrated significant differences (p < 0.05). The mean OAL in the daylight group was determined as 3.64 +/- 0.40 mm, as 3.70 +/- 0.35 mm in the red group, as 3.53 +/- 0.34 mm in the green group and as 3.42 +/- 0.29 mm in the blue group. The mean OAL in the blue group was significantly shorter compared to the red (p = 0.003) and the daylight groups (p = 0.02). When compared with the long wavelength light and indoor daylight, the effect of short wavelength light on OAL in fish was observed to be negative. Exposure to light with modified SS of in indoor environments may be effective in stopping ocular elongation. (C) 2016 Elsevier Ltd. All rights reserved.
The Effects of Light Sources With Different Spectral Structures on Ocular Axial Length in Rainbow Trout (Oncorhynchus Mykiss)
(Academic Press Ltd- Elsevier Science Ltd, 2023) Timucin, Ozgur Bulent; Arabaci, Muhammed; Cuce, Ferhat; Karatas, Boran; Onalan, Sukru; Yasar, Muhterem; Karadag, M. Fatih
Genetic Diversity of Gilthead Seabream (Sparus Aurata) Broodstocks as Determined by Rapd-Pcr
(Soc Israeli Aquaculture & Marine Biotechnology, 2007) Bilgen, Guldehen; Akhan, Suleyman; Arabaci, Muhammed; Oguz, Ismail
The objective of this study was to determine the genetic diversity in gilthead seabream broodstocks from two hatcheries on the northern Aegean Coast of Turkey by RAPD-PCR. Forty primers were tested for each broodstock. Twenty-five produced scorable RAPID bands in stock from the Seferihisar hatchery and 28 in stock from the Aliaga hatchery. Nineteen revealed patterns with scorable amplified primers in both broodstocks. Depending on primer, the number of bands varied 3-16, ranging in size from 438 to 2520 base pairs (bp). The average genetic similarity within stocks was 0.466 for the Seferihisar stock and 0.617 for the Aliaga. The average genetic similarity between the two broodstocks was 0.420, lower than the values within the broodstocks, and the genetic distance between the two broodstocks was 0.245.
The Influence of Stocking Density on Growth and Feed Efficiency in Gilthead Seabream, Sparus Aurata
(Medwell online, 2010) Yilmaz, Yasin; Arabaci, Muhammed
The effects of the stocking density on growth performance and feed efficiency in gilthead seabream Sparus aurata were investigated at cage culture conditions. Three stocking density (36, 40 and 44 fish m(-3)) were tested in the experiment lasted eleven months (from 1 January to 5 December 2006). Replication number was three in low stocking density group (36 fish m(-3)) two in medium density group (40 fish m(-3)) and three in high density group (44 fish m(-3)). A total of 2000 gilthead seabreams with a mean initial body weight of 36.4 +/- 0.4 g (mean SE) were used in the experiment. Feeding regime was applied with commercial feeds as ad libitum. Weight gain, specific growth rate, thermal-unit growth coefficient increased linearly, daily feed intake, feed conversion rate decreased quadratically and protein efficiency rate increased quadratically with increasing stocking density in the range of 36-44 fish m(-3). Results indicated that stocking density of 44 fish m(-3) cause to improve on growth, feed efficiency and gross benefit compared to stocking densities of 36 and 40 fish m(-3) in gilthead seabreams.
Investigation for Zoonotic Disease Pathogens (Aeromonas Hydrophila, Pseudomonas Fluorescens, Streptococcus Iniae) Seen in Carp Farms in Duhok Region of Northern Iraq by Molecular Methods
(Amer inst Physics, 2017) Mohammed, Kamiran Abdulrahman; Arabaci, Muhammed; Onalan, Sukru
The aim of this study was to determine the zoonotic bacteria in carp farms in Duhok region of the Northern Iraq. Carp is the main fish species cultured in the Duhok region. The most common zoonotic bacteria generally seen in carp farms are Aeromonas hydrophila, Pseudomonas fluorescens and Streptococcus iniae. Samples were collected from 20 carp farms in the Duhok Region of the Northern Iraq. Six carp samples were collected from each carp farm. Head kidney tissue samples and intestine tissue samples were collected from each carp sample. Than head kidney and intestine tissue samples were pooled. The total bacterial DNA extraction from the pooled each 20 head kidney tissue samples and pooled each 20 intestinal tissue samples. Primers for pathogens were originally designed from 16S Ribosomal gene region. Zoonotic bacteria were scanned in all tissue samples by absent /present analysis in the RT-PCR. After RT-PCR, Capillary gel electrophoresis bands were used for the confirmation of the size of amplicon which was planned during primer designing stage. As a result, one sample was positive in respect to Aeromonas hydrophila, from intestine and one carp farm was positive in respect to Pseudomonas fluorescens from intestine and two carp farms were positive in respect to Streptococcus iniae. Totally 17 of 20 carp farms were negative in respect to the zoonotic bacteria. In conclusion the zoonotic bacteria were very low (15 %) in carp farms from the Duhok Region in the Northern Iraq. Only in one Carp farms, both Aeromonas hydrophila and Pseudomonas fluorescens were positive. Also Streptococcus inia were positive in two carp farms.
The Investigation of The Presence of Some Bacterial and Viral Agents in Pearl Mullet (Chalcalburnus Tarichii, Pallas 1811) by Real-Time Pcr and The Histopathological Examination
(Parlar Scientific Publications (p S P), 2018) Gulaydin, Ozgul; Ozturk, Cihat; Onalan, Sukru; Karapinar, Zeynep; Arabaci, Muhammed; Ekin, Ismail Hakki; Ilhan, Fatma
The aim of this study was to identify some bacterial (Vibrio cholerae, Listonella anguillarum, Yersinia ruckeri, Aeromonas hydrophila, Aeromonas sobria and Aeromonas caviae) and viral (infectious pancreatic necrosis virus, viral hemorrhagical septicemia virus and infectious hematopoietic virus) pathogens by comparing the bacteriological culture and molecular methods with histopathological examination of various tissues seen in Pearl mullet from freshwater rivers inflowing to the Van lake. Accordingly, bacterial and viral agents were detected in a total of 180 pearl mullet samples by bacteriological culture and Real-Time PCR methods. The samples were collected from six different freshwater rivers that flows to Van lake by random sampling. In the study, Vibrio cholerae, Listonella anguillarum, Yersinia ruckeri, Infectious Pancreatic Necrosis Virus, Viral Hemorrhagic Septicemia Virus and Infectious Hematopoietic Necrosis Virus could not be isolated. However, Aeromonas spp. was identified by bacteriological culture and Real Time PCR methods at 22.22% and 53.33% respectively. The most dominant species was observed to be Aeromonas hydrophila among all identified Aeromonas spp. In histopathological examinations of Aeromonas spp. positive samples, disorders were detected in gill, liver, spleen and kidney tissues. Telangiectasia, edema, hyperplasia and adhesions were observed on the seconder laminates of gills. In liver, the order of the Remak cords were disarranged, and degenerative changes formed in hepatocytes. Melano-macrophages loaded with hemosiderin were intensively detected in all Aeromonas positive samples. As a result, the risk of motile Aeromonas infections in the pearl mullet living in freshwaters flowing into the Van lake is still highly and this could lead to serious adverse effects on human health. To prevent these effects, attention must be paid to hygiene conditions and cold chain in fish served for consumption, the and rivers must be protected from pollution.
Investigation of Zoonotic Disease Pathogens (Aeromonas Hydrophila, Pseudomonas Fluorescens, Streptococcus Iniae) Seen in Carp Farms in the Northern Iraq-Erbil Region by Molecular Methods
(Amer inst Physics, 2017) Ibraheem, Azad Saber; Onalan, Sukru; Arabaci, Muhammed
The aim of this study was to determine the zoonotic bacteria in carp farms in the Northern Iraq-Erbil region. Carp is the main fish species cultured in Erbil region. The most common zoonotic bacteria generally seen in carp farms are Aeromonas hydrophila, Pseudomonas fluorescens and Streptococcus iniae. Samples were collected from 25 carp farms in the Northern Iraq-Erbil region. Six carp samples were collected from each carp farm. Head kidney and intestine tissue samples were collected from each carp sample. Then head kidney and intestine tissue samples were pooled separately from each carp farm. Total bacterial DNA had been extracted from the 25 pooled head kidney and 25 intestinal tissue samples. The pathogen Primers were originally designed from 16S RNA gene region. Zoonotic bacteria were scanned in all tissue samples with absent/present analysis by RT-PCR. Furthermore, the capillary gel electrophoresis bands were used for confirmation of amplicon size which was planned during primer designing stage. As a result, thirteen carp farms were positive in the respect to Aeromonas hydrophila, eight carp farms were positive from head kidney and six carp farms were positive from the intestine, only one carp farm was positive from both head kidney and the intestine tissue samples. In the respect to Streptococcus iniae, four carp farms were positive from head kidney and two carp farms were positive from the intestine. Only one carp farm was positive in the respect to Pseudomonas fluorescens from the intestine. Totally, 9 of 25 carp farms were cleared (negative) the zoonotic bacteria. In conclusion, the zoonotic bacteria were high (64 %) in carp farms in the Northern Iraq-Erbil region.
Phenotypic Differences Between Lactococcus Garvieae Isolates Obtained From Rainbow Trout Farms in Turkey
(Zoological Soc Pakistan, 2020) Onalan, Sukru; Arabaci, Muhammed; Cagirgan, Hasmet
Lactococcus garvieae is one of the main pathogenic agents in rainbow trout farms in Turkey. Twenty-two L. garvieae isolates obtained from different regions in Turkey were evaluated phenotypically in the study. In all isolates, cream colored, bright, round and S-type colonies with smooth margins were seen in TSA medium. They were alive during native examination without movement. It was observed that morphologically all isolates were Gram (+), alpha-hemolytic (BA), oxidase and catalase negative and were reproduced under 21, 37 and 45 degrees C temperatures with 0-6.5 % NaCl salinity. As a result of the examination of biochemical properties with API Rapid ID 32 Strep test, it was observed that 2 L. garvieae isolates were different from other isolates in respect of sucrose utilization. 1 and 19 number isolates were negative for sucrose whereas other isolates gave positive results. 1 number isolate was different from other isolates based on maltose profile. While isolate 22 was maltose negative, the other isolates gave positive results. According to phenotypic differences, all isolates used in the study were classified as three different groups.
A Review on Population Characteristics of Gilthead Seabream (Sparus Aurata)
(Medwell online, 2010) Arabaci, Muhammed; Yilmaz, Yasin; Ceyhun, Saltuk Bugrahan; Erdogan, Orhan; Dorlay, Hakan Galip; Diler, Ibrahim; Koncagul, Seyrani
S. aurata is a marine fish living along the Atlantic coasts of Europe and the Mediterranean Sea. It is an important food species, so that its production reached 128,943 t in the European aquaculture in 2008. However, little is known on its biology, in particular the efective and census sizes, distribution, spawning grounds and behaviour. Also the species subdivision still needs to be clarified. In this review, studies related with gilthead sea bream populations and problems with the clarification of gilthead seabream population structure in hatchery and wild were reviewed. Also, it was intensified on potential research areas, having importance in determination of population structure of gilthead seabream such as morphology and behaviour in hatchery and wild. Determination of the genetic structure of S. aurata populations based on behaviour and morphology will not only contribute greatly to biological clarification of the species but also will help the development strategy for conservation of natural stocks (e.g., protecting the stopover points) and the determination of reference stocks, which is important for the culture of gilthead seabream to use in the genetic improvement programmes.
A Study on Spawning Induction in Grass Carp (Ctenopharyngodon Idellus Valenciennes, 1844) by Using Different Combinations of Lhrha ([d-Ser(but)6,pro9< and Domperidon
(Kafkas Univ, veteriner Fakultesi dergisi, 2009) Arabaci, Muhammed
In this study, the effects of single administration of [D-Ser(But)(6),Pro(9)-NEt]-LHRHa at high dose alone (50 mu g kg(-1)), and at different doses (10, 20 mu g kg-1) combined with constant dose of the dopamine receptor antagonist, Domperidon (10 mg kg(-1)), for induction of spawning in the grass carp broodstocks were determined under local hatchery conditions, and compared with classic Carp Pituitary Extract (CPE, double injection) application (Water temperature; 25 degrees C). Physiological salina (0.7% NaCl) injected fish were used as control group. No spawning was observed in the control group. Spawning ratio was low in LHRHa at high dose alone treatment group (50 mu g kg(-1)), and in 10 mu g kg(-1) dose of LHRHa at combined with 10 mg kg(-1) dose of DOM treatment group. Spawning ratio was high in 20 mu g kg(-1) dose of LHRHa combined with 10 mg kg(-1) dose of DOM treatment group, and in CPE treatment group. Latency period was shorter approximately two times in LHRHa+DOM treatment groups than CPE treatment groups (9-10 h, 17 h respectively). There was no differences between all spawning groups in respect of spawning index of broodstocks, and fertilisation rates of eggs (P>0.05). As a result, spawning was induced successfully in grass carp broodstocks by 20 mu g kg(-1) [D-Ser(But)(6),Pro(9)-NEt]-LHRHa+10 mg kg(-1) DOM treatment, in a single injection, in a shorter latency period and without decreasing in egg quality, comparing to CPE treatment. Application of this combination can be useful for healthy broodstock and hatchery management in grass carp culture.