Browsing by Author "Aras, Zeki"

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Microbial Contamination of in Vitro-Derived Cattle Embryos and Resistance Genes
(Sciendo, 2025) Golen, Gokcenur Sanioglu; Akar, Kadir; Karasahin, Tahir; Senturk, Goktug; Golen, Selcuk; Aras, Zeki
The global trend of in vitro embryo systems, particularly the in vitro fertilization (IVF) culture system, is gaining momentum. Despite the strict standards followed in in-vitro embryo procedures, microbiological contamination is occasional, and the relevant literature is scarce. In this study, for the first time, IVF culture dishes with microbial contamination and resistance genes of isolates were evaluated in veterinary medicine. Samples were microscopically taken from IVF tissue cultures suspected of bacterial or fungal contamination and sent to the microbiology laboratory for further examination. The total contamination rate was 11.1% in IVF cultures where cell division did has stopped or turbidity occurred. Identification of contaminant microorganisms showed that infections were mainly caused by E. coli 9.5% and Candida spp. 1.58%. A set containing multiplex antibiotic primers was used during the IVF protocol to determine antibiotic resistance genes. All E. coli isolates were resistant to penicillin used in the Kirby-Bauer, and 16% was resistant to streptomycin. This study is the first systematic evaluation of microbial contamination of bovine IVF culture vessels in veterinary medicine. IVF culture should be evaluated in more detail to learn more about the source of the microorganism and to develop adequate measures to prevent microbial contamination.
A Novel Polymerase Chain Reaction To Detect Brucella Canis in Dogs
(Kafkas Univ, veteriner Fakultesi dergisi, 2015) Aras, Zeki; Taspinar, Mehmet; Aydin, Ibrahim
In this study, the specific polymerase chain reaction has been standardized and evaluated for the direct diagnosis of Brucella canis in vaginal swab samples from dogs. The specific primer sets are directed to the 16S-23S rRNA inter-space region of Brucella spp. and the deletion of 351 bp in BMEI1426-BMEI1427 in B. canis. A total of 21 references and field strains and 35 vaginal swab samples were used for the evaluation of the polymerase chain reaction. It found that polymerase chain reaction is positive for B. canis DNA indicated by only amplification of 214 bp product. It detected at least 2.7 x 10(1) CFU/g of bacteria diluted in vaginal swab samples indicates that the polymerase chain reaction can be used as a practical alternative for bacterial isolation. The novel polymerase chain reaction provides a simple and rapid for the detection of B. canis in clinical and field samples in one step and in short time about 24 h.