Browsing by Author "Arslantaş, Ali"

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2, 10, 16, 24-tetrakis 4- (2-fenoksi-l, 3-diokso-2,3-dihidro-lh-inden-2-iloksi) Ftalonitril İçeren Kobalt (Ii) Bileşiğinin Dna Bağlanmasının Spektroskopik Yöntemler ile Araştırılması
(2017) Agirtas, M. Salih; Arslantaş, Ali
2,10, 16, 24-tetrakis 4- (2-fenoksi-1,3-diokso-2,3-dihidro-lH-inden-2-iloksi) ftalonitril içeren önceden sentezlenmiş kobalt (II) bileşiğinin (PcCo) CT-DNA ile bağlanma özellikleri UV/Vis absorpsiyon, floresan titrasyonu yöntemleri kullanılarak bağlanma özelliklerini belirlemek için araştırıldı. DMF çözücü ortamında ve DNA'nın yokluğunda, PcCo'nun absorpsiyon titrasyon spektrumu, Q-band için yaklaşık 670 nm'de pik ve B -bandı için 340 nm de pik verir. Calf thymus-DNA, PcCo varlığında PcCo'nun emisyon yoğunluğu yaklaşık 545 nm'de emisyona neden olur. Termal denatürasyon profili çalışması UV-Vis spektroskopik tekniği ile sistematik olarak incelenmiş ve Co (II) ftalosiyanin bileşiği için erime noktası sıcaklık değeri 7.87 oC civarında bulunmuştur. CTDNA yokluğunda ve varlığında PcCo'in redüksiyon oksidasyon davranışı, dönüşümlü voltametri (CV) ile incelendi. PcCo için katodik pik ve anodik pik potansiyelleri, EPc için 0.84, -0.36 V ve 0.88 V, 0.15 V ve EPa için -0.69 V olarak kaydedildi. Dönüşümlü (cyclic) voltametri sonuçlar, bir yarı-tersinir redaksiyon oksidasyon dalgasını göstermektedir. Epc ve Epa potansiyelindeki değişim, PcCo'in CT-DNA'yla güçlü etkileşim gösterdiğini önermektedir. PcCo'in CT-DNA ile olan etkileşimi ayrıca jel elektroforez yöntemi kullanılarak araştırılmıştır. Jel elektroforezi sonuçları, CT-DNA bantlarının yoğunluğunda azalma olduğunu gösterdi. Tüm bulgular, PcCo ile CT-DNA molekülü arasındaki etkileşimin interkalatif bağlanma türü olduğunu göstermektedir.
Interaction Activity of Metal-Free Phthalocyanine Compound Bearing Tetra -(2-(N-2'-Cyanoethyl)aminoethylsulfanyl) Units with DNA
(Chemical Society of Pakistan, 2025) Arslantaş, Ali; Aǧirtaş, Mehmet Salih
The phthalocyanine having 4-(2-(N-2'-cyanoethyl)aminoethylsulfanyl) group had been reported earlier in the literature. In this current studying, DNA binding activity of 1Pc phthalocyanine bearing 4-(2-(N-2'-cyanoethyl)aminoethylsulfanyl units was examined spectroscopically via elctronic absoption, fluorescence titration, melting point profile, electrophoresis and viscosity methods. The interaction activity of 1Pc compound was examined at differing concentrations. UV/Vis spectrometer, viscosity, fluorescence spectroscopy and thermal melting temperature confirmed that 1Pc binds to the DNA. The Kb of 1Pc is also estimated via UV/Vis titration and Kb of 1Pc was computed as 2.1394 x 106 M-1. The Kb value demonstrated that 1Pc reacts with DNA by an intercalative mechanism. Alongside this research, the mechanism by which the compound binds to DNA was investigated by determining Tm. The Tm of DNA + 1Pc complex was identified as 74.31. This data confirmed that 1Pc binds to DNA intercalatively. All the results obtained from the used methods demonstrated that 1Pc phthalocyanine compound has an efficient DNA interaction activity and 1Pc phthalocyanine compound interacts with DNA via an intercalative mechanism. As a result, the compound may be a therapeutic agent due to its DNA interaction property. © 2025 Elsevier B.V., All rights reserved.
An Investigation Upon Dna Interaction of 2 (3), 9 (10), 16 (17), 23 (24) –tetrakis 4-(4 Phenoxy) Phthalocyanine Compound
(2020) Cihan, Durmuş; Agırtas, Mehmet Salıh; Arslantaş, Ali
The DNA binding activity of previously synthesized and characterized 2(3), 9(10), 16(17), 23(24) –Tetrakis 4-(4-(2-phenylprop-2-yl) phenoxy) phthalocyanine compound (PcF) with CT-DNA was studied using UV/Vis, emission spectroscopic titrations,the melting temperature, viscosity measurement, and agarose gel electrophoresis methodsin a Tris-HCl buffer solution at a pH of 7.1 at room temperature. The absorption titrationspectra studies of PcF showed that absorbance intensities were decreased with increasing ofconcentrations of CT-DNA and the finding proved that the compound interacts with theDNA. Addition to absorption titration study, emission spectroscopic titration, the meltingtemperature, viscosity measurement, and agarose gel electrophoresis methods were alsoperformed to investigate the binding activities of PcF with the DNA. The results of thesemethods confirmed the findings of absorption spectra study that the compound interactswith the DNA
Study on Dna Binding Properties of Nickle (Ii) Phthalocyanine Compound With 2-Isopropyl Methylphenoxy Substituents
(2017) Arslantaş, Ali; Agirtas, M. Salih
I n this study, previously synthesized Ni (II) phthalocyanine compound bearing 2,10,16,24-tetrakis(2-isopropyl-5-methylphenoxy group was chosen for its interaction with calf thymus-DNA. Calf thymus-DNA was used to determine DNA binding properties of Ni (II) phthalocyanine compound. The DNA binding activities of Ni (II) phthalocyanine compound bearing 2-isopropyl-5-methylphenoxy substituent was investigated by using absorption titration, fluorescence emission, cyclic voltammetry, gel elctrophoresis in Tris-HCl buffer at pH 7.0. In addition to above methods, melting point and viscosity experiment were performed to determine the DNA intercation of the compound in Tris-HCl buffer solution. The results showed that Ni (II) phthalocyanine compound binds strongly to calf thymus-DNA via intercalation binding