Browsing by Author "Aydin, Firat"

Now showing 1 - 5 of 5

Determination and Comparison of Metal Contents in Simulated Body Fluid Medium Conditions of the Plant Species by Flame Atomic Absorption Spectrometry (Faas)
(Marmara Univ, 2022) Umaz, Adil; Aydin, Firat; Tokul Olmez, Ozge; Firat, Mehmet; Ozturk, Mehmet; Ertas, Abdulselam; Aydin, Isil
The importance of plants in folk medicine and scientific studies is increasing day by day. In this context, the plants pose a danger to human health when they are eaten by the public, consumed as herbal tea or used as medicinal plants especially the toxic metals in their composition. For this reason, determining the macro, micro and toxic element content in the plants is important for health. In this study; the metal contents of root and aerial parts of nine different Salvia species were determined and the chemometric evaluation of the obtained results was made. In the principal component analysis (PCA) made with 20 elements of Salvia species, it was determined that the first two principal components explained 62.60% of the variance and the first six principal components explained 92.70%. When PCA and hierarchical cluster analysis (HCA) results are evaluated; the parts of Salvia species root and aerial parts were determined that were not clearly separated and there was no regional grouping. In addition, Salvia species prepared as herbal tea was left to different simulated body fluid medium conditions, and the changes in metal contents were examined. It was determined that were taken up Cr, Cd, Pb, Cu, Zn metals in the simulated saliva fluid (SSF), Ni, Mn metals in the simulated gastric fluid (SGF), and Na, K metals in the simulated intestinal fluid (SIF). It was determined that Fe metal in almost all Salvia species was not taken from three simulated body fluid mediums. Thus, elements determined which were taken up in body fluid mediums.
Effects of Dietary Boron on Cervical Cytopathology and on Micronucleus Frequency in Exfoliated Buccal Cells
(Wiley, 2007) Korkmaz, Mehmet; Uzgoren, Engin; Bakirdere, Sezgin; Aydin, Firat; Ataman, O. Yavuz
Recent evidence indicates that boron and borates may have anticarcinogenic properties. In this study, we have investigated the incidence of adverse cytological findings in cervical smears and the micronucleus (MN) frequency in women living in boron-rich and boron-poor regions. Cervical smears were prepared from 1059 women with low socioeconomic status; 472 of the women lived in relatively boron-rich rural areas, while 587 lived in relatively boron-poor regions. The average and standard deviation values for the age of the women screened with the cervical Pap smear test were 41.55 +/- 8.38. The mean dietary intake of boron was 8.41 mg/day for women from the boron-rich regions, and 1.26 mg/day for women living in the boron-poor regions (P < 0.0001). Women from the boron-rich regions had no cytopathological indications of cervical cancer, while there were cytopathological findings for 15 women from the boron-poor areas (chi(2) = - 10.473, P < 0.05). Sixty women, 30 from each region, were chosen for evaluating MN frequencies in exfoliated buccal cells. MN frequencies for women from the boron-rich and boron-poor regions were not significantly different (t = -0.294, P > 0.05). Also, there were no significant correlations between age and MN frequency for women from both the boron-rich (r = 0.133, P = 0.48, P > 0.05) and boron-poor (r = -0.033, P = 0.861, P > 0.05) regions. The results suggest that ingestion of boron in the drinking water decreases the incidence of cervical cancer-related histopathological findings. There was no correlation between the pathological findings from the cervical smears and buccal cell MN frequency suggesting that the two study populations were exposed equally to gentotoxic agents. Nonetheless, cervical cancer-related histopathological findings should be validated by other researchers. (c) 2007 Wiley Periodicals, Inc.
Farklı Familyalara Ait Türlerdeki Element Analizlerinin İndüktif Eşleşmiş Plazma Kütle Spektrometrisi (Icp-ms) ile Belirlenmesi ve Karşılaştırılması
(2022) Umaz, Adil; Fırat, Mehmet; Aydin, Firat; Ertaş, Abdulselam
Bitkilerin tüketimi ve tıbbi amaçlı kullanımları her geçen gün artmaktadır. Bu nedenle bitki içeriklerinin bilinmesi insan sağlığı açısından önemlidir. Bu çalışmada dört farklı familyaya ait on dokuz türün çoklu element içerikleri belirlenmiştir. Bu familyalara ait aynı cinsin farklı türlerinin çoklu element içerikleri karşılaştırılmıştır. Ayrıca, aynı cinsin farklı kısımlarının element içerikleri karşılaştırılmıştır. Aynı cinsin farklı türlerinin ve aynı türün farklı kısımlarının element içerikleri birbirinden farklı olduğu tespit edilmiştir. Ayrıca, türlerin Cd ve Pb içerik sonuçları Dünya Sağlık Örgütü'nün (WHO) ham bitki materyalinde izin verilen maksimum konsantrasyon değerleri ile karşılaştırılmıştır. Tüm türlerde Cd içeriği WHO tarafından belirlenen değerden yüksek bulunmuştur. Yalnızca, Sternbergia clusiana Boiss'in Pb içeriği. (A3) WHO tarafından tespit edilen değerden daha yüksek olduğu tespit edildi.
An Investigation and Comparison of Concentration Change in Simulated Body Fluid Medium Conditions of the Calcium Element in 27 Different Salvia Species
(Istanbul Univ, Fac Pharmacy, 2023) Umaz, Adil; Aydin, Firat; Firat, Mehmet; Yener, Ismail; Ertas, Abdulselam; Elmastas, Ayhan
Background and Aims: In this study, calcium (Ca) element concentration changes of Salvia species in simulated body fluid (SBF) medium conditions were investigated and the results in these medium conditions were compared with each other.Methods: Salvia species samples were air-dried and ground into powder. Salvia species was prepared as a herbal tea. Pre-pared teas were left in three different SBF medium conditions. The samples were analyzed using Flame Atomic Absorption Spectroscopy (FAAS) method to determine the Ca absorptions. Also, a correlation analysis of the results obtained in three different SBF medium conditions and the species in simulated gastric fluid (SGF) medium conditions was performed.Results: When the SBF medium conditions were compared, it was determined that the highest Ca absorption of all Salvia species occurred in the SGF conditions. Thus, it can be said that the Ca in plants and foods occurs in the gastric fluid medium and its absorption occurs there. When the result of the correlation analysis was evaluated, it was determined that there was a stronger correlation between the SGF and simulated intestinal fluid (SIF) mediums compared to other mediums.Conclusion: The Ca absorption was determined according to what remained in the SGF the most. Based on the results ob-tained from SBF medium conditions, it can be said which element is taken in which body fluid medium. Information on the differences between samples belonging to different SBF medium conditions was not obtained. However, when the simulated fluid medium conditions were evaluated individually, information was obtained for two or more samples.
Method Validation of 15 Phytochemicals Inhypericum Lysimachioidesvar.spathulatumby Lc-ms/Ms, and Fatty Acid, Essential Oil, and Aroma Profiles With Biological Activities
(Springer, 2020) Akdeniz, Mehmet; Yilmaz, Mustafa Abdullah; Ertas, Abdulselam; Yener, Ismail; Firat, Mehmet; Aydin, Firat; Kolak, Ufuk
The aim of the present study was to develop and validate a LC-MS/MS method for quantification of 15 phytochemicals inHypericumspecies. The developed method was fully validated in terms of repeatability (inter-day and intra-day precision), limits of detection and quantification, linearity, recovery and relative standard uncertainty. The developed and validated LC-MS/MS method was applied to determine 15 phytochemicals in the ethanol extracts ofH. lysimachioidesvar.spathulatumaerial parts (HLS-A) and roots (HLS-R). Hyperoside was found to be the major compound in HLS-A and HLS-R ethanol extracts (16,560.3 and 3561.6 mu g analyte/g extract, respectively). According to the results of GC-MS analyses,cis-13,16-docosadienoic acid (35.0%), caryophyllene oxide (24.33%) and undecane (28.21%) were determined as the major components in fatty acid, essential oil and aroma compositions ofH. lysimachioidesvar.spathulatum, respectively. HLS-A and HLS-R ethanol extracts showed moderate activity in ABTS cation radical decolorization assay. The major components (hyperoside, astragalin, and quercetin) present in HLS-A and HLS-R ethanol extracts were found to have also the highest antioxidant effect in ABTS cation radical scavenging method. The extracts, main constituents in the extracts and essential oil had no toxic-cytotoxic potential against PDF, MCF-7 and HT-29 cell lines. HLS-A ethanol extract and essential oil exhibited high butyrylcholinesterase inhibitory activity. Quercetin showed the highest inhibitory effect against acetyl- and butyryl-cholinesterase, urease and tyrosinase among the tested samples. According to biological activity studies,H. lysimachioidesvar.spathulatumand their major components might be promoted as promising sources of natural agents and used in the development of nutraceuticals, functional food ingredients and pharmaceutical industry.