Browsing by Author "Balahoroglu, Ragip"

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A Biochemical Study on the Protective Potential of Centaurium Erythraea L. in Rats at Acute Aspirin Exposure
(Parlar Scientific Publications (p S P), 2011) Ozkol, Halil; Tuluce, Yasin; Koyuncu, Ismail; Balahoroglu, Ragip
The aim of this study was to determine protective effect of Centaurium erythraea L. on rats exposed to aspirin (ASA). The protective influence of the 50% aqueous-ethanolic Centaurium erythraea L. extract (AECE) (100 mg/kg body weight) was investigated in rats exposed to a single dose of 200 mg/kg body weight ASA administration. Twenty-one Sprague-Dawley albino rats were distributed into three groups equally (n=7) as follows: A (control), B (only ASA-treated) and C (ASA+AECE-treated) group. At the end of 4 h ASA administration to groups B and C, some serum biochemical constituents were assigned and compared between the groups. Results showed that levels of aspartate aminotransferase, alanine aminotransferase, creatinine and potassium increased in group B compared with the control group, but they were decreased significantly with AECE supplementation. While lipase activity decreased only in group B, level of uric acid decreased both in groups B and C compared with control group. In conclusion, the current trial has shown that treatment of AECE with ASA supplied relative protection against acute ASA toxicity through ameliorating some blood parameters.
Characteristics of Resveratrol and Serotonin on Antioxidant Capacity and Susceptibility To Oxidation of Red Blood Cells in Stored Human Blood in a Time-Dependent Manner
(Sage Publications Ltd, 2018) Huyut, Zubeyir; Sekeroglu, Mehmet Ramazan; Balahoroglu, Ragip; Huyut, Mehmet Tahir
Objective In stored red blood cells (RBCs), which are used in diseases (e.g., acute blood loss and leukaemia), storage lesions arise by oxidative stress and other factors over time. This study investigated the protective effects of resveratrol and serotonin on stored RBCs. Methods Blood from each donor (n=10) was placed in different bags containing 70mL of citrate phosphate dextrose (total volume: 500mL) and divided into three groups (n=30): control, 60 mu g/mL resveratrol, and 60 mu g/mL serotonin. Malondialdehyde (MDA) and glutathione (GSH) levels, activity of glutathione peroxidase (GSH-Px), catalase, and carbonic anhydrase (CA), and susceptibility to oxidation in RBCs, and pH in whole blood were measured at baseline and on days 7, 14, 21, and 28. Results MDA levels and susceptibility to oxidation were increased in all three groups time-dependently, but this increase was greater in the serotonin group than in the other groups. Activity of GSH-Px, CAT, and CA, as well as GSH levels, were decreased in the control and serotonin groups time-dependently, but were significantly preserved in the resveratrol group. The pH was decreased in all groups time-dependently. Conclusion Our study shows that resveratrol attenuates susceptibility to oxidation of RBCs and protects their antioxidant capacity, and partially preserves CA activity time-dependently.
Comparison of Bacterial Culture and Microscopic Urine Analyzer in the Urinary Tract Infections
(Aves, 2013) Guducuoglu, Huseyin; Bektas, Abdullah; Gultepe, Bilge; Balahoroglu, Ragip; Bayram, Yasemin
Objective: The urine culture is the gold standard for diagnosis of urinary tract infection. Fully automated urine analyzer (FAUA) which gives quantitative results is also commonly used for urinalysis. This device identifies erythrocytes, leukocytes and epithelial cells as well as bacteria and renal elements performing a microscopic analysis. The aim of this retrospective study is to compare urine cultures with the FAUA used by the clinicians for rapid diagnosis of urinary tract infections. Methods: A total number of 23 065 urine specimens sent from various clinics to the Microbiology and Clinical Microbiology Laboratory in our hospital for both urinalysis and urinary culture between the dates of 1 June 2010 and 31 October 2011 were evaluated. Urinalysis was performed with iQ (R) 200 (Iris Diagnostics, Chatsworth, CA, USA). The FAUA values with >= 5 leukocytes and >= 1 bacteria were classified as FAUA-positive. Urine cultures growing >= 5x10(4) cfu/ml were included in the comparison. Results: Of the total FAUA and culture results, 11.7% were incompatible in terms of the number of leukocytes including leukocytenegative/culture-positive (0.4%) and leukocyte-positive/culturenegative (11.3%) results, and 6.6% were incompatible in terms of the number of germs including culture-positive/germ-negative (5%) results and germ-positive/culture-negative (1.6%) results. Conclusions: FAUA often used by clinicians for rapid diagnosis of urinary tract infection will contribute to the evaluation of the culture in a more efficient way, however, it should not replace the urine culture.
Determining Oxidant and Antioxidant Status in Patients With Genital Warts
(Taylor & Francis Ltd, 2015) Cokluk, Erdem; Sekeroglu, Mehmet Ramazan; Aslan, Mehmet; Balahoroglu, Ragip; Bilgili, Serap Gunes; Huyut, Zubeyir
Objectives: Warts are abnormal skin growths caused by human papilloma virus (HPV) infections within the skin of patients. Genital warts usually appear in the perianal and perigenital regions. Asymptomatic warts may be activated after years and may damage natural immunity. The inflammation that occurs during this process may lead to an imbalance between the prooxidant and the antioxidant systems. The aim of this study was to investigate erythrocyte glutathione peroxidase (GSH-Px) activity, serum paraoxonase enzyme levels, and oxidative stress levels in patients with genital warts. Patients and Methods: In total, 32 patients with genital warts and 35 healthy subjects were included in this study. Erythrocyte GSH-Px activity, serum catalase activity, and paraoxonase enzyme, and malondialdehyde (MDA) levels were determined. Results: Erythrocyte GSH-Px activity, serum MDA levels, and catalase activity were significantly higher in patients with genital warts than in controls (P < 0.01, P < 0.05, and P < 0.05, respectively). However, serum paraoxonase enzyme levels were not significantly different between groups (P > 0.05). Serum triglyceride levels were significantly lower in patients with genital warts than in controls (P < 0.01). However, there were no statistically significant differences between groups with respect to total cholesterol, high-density lipoprotein cholesterol, or low-density lipoprotein cholesterol levels (all P > 0.05). Conclusions: Our data suggest that oxidative stress is increased in genital warts. Increased oxidative stress levels may contribute to the pathogenesis of genital warts, and prolonged HPV infection due to chronic inflammation could also affect oxidative stress.
The Effect of Different Doses of Isotretinoin on Pituitary Hormones
(Karger, 2015) Karadag, Ayse Serap; Takci, Zennure; Ertugrul, Derun Taner; Bilgili, Serap Gunes; Balahoroglu, Ragip; Takir, Mumtaz
Background: There are a limited number of studies investigating the side effects and effectiveness of various doses of isotretinoin (ISO). We have previously shown that high-dose ISO affects pituitary hormones. Objectives: To our knowledge, there is no study in the literature looking into the effects of various doses of ISO on pituitary hormones. We searched pituitary hormones in three groups of different doses in acne patients. Methods: We included 105 acne vulgaris patients from two different centers. We divided the patients into three groups; the first group received 0.5-1 mg/kg/day, the second 0.2-0.5 mg/kg/day and the third intermittent 0.5-1 mg/kg/day (only 1 week in 1 month) ISO treatment. Blood samples were collected for biochemistry and hormone analysis, before the treatment and after 3 months. Results: After 3 months of treatment with ISO, luteinizing hormone (LH) (p < 0.001), prolactin (p < 0.001), total testosterone (p < 0.001), adrenocorticotropic hormone (ACTH) (p < 0.001), cortisol (p < 0.001), insulin-like growth factor-binding protein 3 (p < 0.001), insulin-like growth factor 1 (IGF-1) (p = 0.002), growth hormone (GH) (p = 0.002) and free T3 (fT3) (p < 0.001) levels had decreased significantly. Furthermore, we split data into three different groups. Among the patients receiving intermittent-dose ISO, LH, ACTH, IGF-1, GH and fT3 measurements lost significance. Most of the significant measurements observed in the whole group were also significant among the patients receiving high-dose ISO. Additionally, dehydroepiandrosterone sulfate (p = 0.003) levels increased, and free T4 levels decreased significantly. Conclusions: ISO affects pituitary hormones at all of these three doses. The differences in pituitary hormones are more pronounced in high-dose treatment. The weakest effect was observed in the intermittent-dose group. Choosing lower doses of ISO may decrease side effects, however the effectiveness of the treatment may also be diminished. ISO, by affecting the PPAR gamma/RXR system, may affecting hormone systems. These changes in various hormone systems may be related to the effectiveness of ISO. (C) 2015 S. Karger AG, Basel
Efficacy and Safety of Rectal Ibuprofen for Patent Ductus Arteriosus Closure in Very Low Birth Weight Preterm Infants
(Taylor & Francis Ltd, 2017) Demir, Nihat; Peker, Erdal; Ece, Ibrahim; Balahoroglu, Ragip; Tuncer, Oguz
Background: To compare rectal ibuprofen with oral ibuprofen for the closure of hemodynamically significant patent ductus arteriosus (hsPDA) in very low birth weight (VLBW) preterm infants.Study design and subjects: In a prospective, randomized study, 72 VLBW infants who had hsPDA received either rectal or oral ibuprofen. The plasma concentration of ibuprofen and renal functions were determined in both groups by the high-performance liquid chromatography (HPLC) method and cystatin-C (cys-C), respectively.Results: The hsPDA closure rate of the group that received rectal ibuprofen was similar to oral ibuprofen (86.1% versus 83.3%) after the first course of the treatment (p=0.745). A statistically significant difference was identified between the mean plasma cys-C levels before and after treatment in both the rectal and oral ibuprofen groups (p=0.004 and p<0.001, respectively). The mean plasma ibuprofen concentration was similar in both groups after the first dose (rectal 44.0612.4; oral, 48.28 +/- 22.8) and the third dose (rectal, 45.34 +/- 24.3; oral, 48.94 +/- 24.8) (p>0.05 for all values).Conclusions: Rectal ibuprofen is as effective as oral ibuprofen for hsPDA closure in VLBW infants. The rise in the cys-C level with rectal and oral treatment shows that patients with borderline renal function should be evaluated and followed closely.
The Levels of Nitrite, Nitrate and Lipid Peroxidation in Diabetic Mouse Brain: the Effect of Melatonin and Pentoxifylline
(Taylor & Francis Ltd, 2022) Yalcinkaya, Ahmet S.; Sekeroglu, Mehmet Ramazan; Huyut, Zubeyir; Cokluk, Erdem; Ozbek, Hanefi; Ozturk, Gurkan; Balahoroglu, Ragip
Objective: This study investigated the relationship between diabetes (DM) and nitrite, nitrate and MDA levels and effect of melatonin and pentoxifylline. Methods: Sixty mice were randomly divided into four groups. Control: no action; Diabetes group (DM): after fasting-blood-glucose (FBG) was measured, 150 mg/kg alloxane was applied intraperitoneally three-times every other day; Diabetes + Melatonin (DM + MLT) and Diabetes + Pentoxifylline groups (DM + PTX): following the same procedures with DM, 10 mg/kg melatonin and 50 mg/kg pentoxifylline were administered subcutaneously six days, respectively. Following FBG analysis, brain tissues were taken under the anaesthesia. Nitrite, nitrate and MDA levels were measured. Results: In the all groups with alloxane, FBG were higher than in before application (p < .05). Also, FBG, nitrite, nitrate and MDA levels in the DM + MLT and DM + PTX groups were lower than in the DM (p < .05). Conclusions: Nitrite and nitrate may be related to etiopathogenesis of DM, and pentoxifylline and especially melatonin relatively decrease nitrite, nitrate and lipid peroxidation.
The Levels of Vitamin B12, Folate and Homocysteine in Mothers and Their Babies With Neural Tube Defects
(Taylor & Francis Ltd, 2016) Peker, Erdal; Demir, Nihat; Tuncer, Oguz; Ustyol, Lokman; Balahoroglu, Ragip; Kaba, Sultan; Karaman, Kamuran
The aim of the present study was to determine the serum levels of vitamin B-12, folate, and homocysteine (Hcy) in mothers and their babies, and to assess the association between these levels and neural tube defect (NTD). The study group included 92 baby-mother pairs, where the babies had NTD, and the control group included 102 pairs, where the babies had no NTD, from May 2012 to May 2015. Plasma vitamin B-12, folate, and Hcy levels of the babies and mothers were measured, and compared with each other. NTD was diagnosed in 2.6% of our babies. The vitamin B-12 levels in the mothers and the babies in the study group were determined as 166.2 +/- 63.7 pg/mL and 240.3 +/- 120.3 pg/mL, and in the control group as 1 9 0 +/- 80.2 pg/mL and 299.5 +/- 151.4 pg/mL, respectively. There was a significant difference between the two groups in terms of both the mothers' and the babies' vitamin B-12 levels (p = 0.024 and p = 0.003, respectively). The plasma folate levels of the mothers in the study group (5.2 +/- 3 ng/mL) were significantly lower than control group (6.4 +/- 4.3 ng/mL, p = 0.032). The plasma Hcy level of the mothers in the study group (9.3 +/- 3.8 mu mol/L) was significantly higher than the control group (7 +/- 3.8 mu mol/L, p<0.001). High plasma Hcy levels and low plasma folate and vitamin B-12 levels are risk factors for NTD. Our results show that the risk for NTD can be decreased by fortification of mothers-to-be, particularly in rural areas with folate and vitamin B-12 deficiency, which would lower the plasma Hcy level.
Protective Effects of Selenium, N-Acetylcysteine and Vitamin E Against Acute Ethanol Intoxication in Rats
(Springernature, 2017) Ozkol, Halil; Bulut, Gulay; Balahoroglu, Ragip; Tuluce, Yasin; Ozkol, Hatice Uce
The aim of this study was to determine possible protective influences of selenium (Se), N-acetylcysteine (NAC), and vitamin E (Vit E) against acute ethanol (EtOH) intoxication. Thirty-six rats were divided into six groups: I (control), II (EtOH), III (EtOH + Se), IV (EtOH + Vit E), V (EtOH + NAC), and VI (EtOH + mix). Except group I, EtOH was given the other pretreated (groups III, IV, V, and VI) and untreated groups (group II). Compared with the EtOH group, serum aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, creatine kinase, and creatine kinase-MB levels were significantly decreased in all pretreated groups, whereas slightly diminished amylase and lipase were observed. Compared with the control group, a remarkably lower total antioxidant status (TAS), but higher total oxidant status (TOS), and oxidative stress index (OSI) were seen in brain, liver, and kidney tissues. The values of these parameters were less affected from EtOH-exposed brain tissue of EtOH + NAC and liver of EtOH + mix groups. Both significant decrease of catalase activity and marked increases of adenosine deaminase and myeloperoxidase were determined only in liver tissue of the EtOH group. Activities of these enzymes were restored in almost all pretreated groups. Moreover, an increase of xanthine oxidase activity was prevented in brain tissue of pretreated groups. In histopathological examination of the liver, hydropic degeneration, sinusoidal dilatation, mononuclear cell infiltration, and marked congestion, which were seen in the EtOH group, were prevented in all pretreated groups. Relative protection against acute EtOH toxicity, in both single and combined pretreatments of Se, NAC, and Vit E supplementation, was probably through antioxidant and free radical-neutralizing effects of foregoing materials.
The Relationship of Oxidation Sensitivity of Red Blood Cells and Carbonic Anhydrase Activity in Stored Human Blood: Effect of Certain Phenolic Compounds
(Hindawi Ltd, 2016) Huyut, Zubeyir; Sekeroglu, Mehmet Ramazan; Balahoroglu, Ragip; Karakoyun, Tahsin; Cokluk, Erdem
It has been reported that many modifications occur with the increase of oxidative stress during storage in erythrocytes. In order to delay these negative changes, we evaluated whether the addition of substances likely to protect antioxidant capacity in stored blood would be useful. Therefore, we investigated the effects of resveratrol, tannic acid, and caffeic acid in lipid peroxidation and antioxidant capacity of erythrocytes in stored blood. Donated blood was taken into four CPD containing blood bags. One bag was used as the control, and the others were supplemented with caffeic acid (30 mu g/mL), resveratrol (30 mu g/mL), and tannic acid (15 mu g/mL), respectively. Erythrocyte lipid peroxidation, sensitivity to oxidation, glutathione levels and carbonic anhydrase, glutathione peroxidase, and catalase activities were measured on days 0, 7, 14, 21, and 28. In the control group, erythrocyte malondialdehyde levels and sensitivity to oxidation were increased whereas glutathione, glutathione peroxidase, and catalase levels were decreased (P < 0.05). Resveratrol and caffeic acid prevented malondialdehyde accumulation and preserved glutathione, glutathione peroxidase, and catalase activities in erythrocytes. We demonstrated that resveratrol, caffeic acid, and tannic acid in stored blood could decrease the sensitivity to oxidation of erythrocytes in vitro but did not exhibit such effects on CA activity.