Browsing by Author "Basbugan, Yildiray"

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Assessment of the Wheatgrass Effects on Antioxidant Capacity and Dna Damage in Rats With Experimental Renal Failure
(Parlar Scientific Publications (p S P), 2019) Mis, Leyla; Comba, Bahat; Basbugan, Yildiray; Yasar, Semih; Comba, Arzu
In this study, it was aimed to evaluate the effect of wheatgrass on antioxidant capacity and DNA damage in rats with renal failure. In the study, 200-300 g 24 healthy Wistar-Albino male rats obtained from YYU Experimental Animal Unit were used. The rats were randomly divided into 4 groups and each group was formed with 6 rats. Groups were allocated as; 1. Control Group, 2. Renal Failure Group (Genta Group) 3. Renal Failure + Wheatgrass Group (Genta +Wheatgrass) 4. Wheatgrass Group. Gentamicin sulfate was given intraperitoneal at a dose of 50 mg/kg for 1 week and once a day to the groups with renal failure (Groups 2 and 3). Wheat grass extract (10 ml/kg) was added to the drinking water of the wheatgrass group rats (3rd and 4th groups) once a day. This practice lasted for 4 weeks. Total oxidant, antioxidant quantities, DNA damage were evaluated at the end of 4 weeks. The result that wheatgrass could be useful in renal failure was reached.
Changes in Serum Adenosine Deaminase and Isoenzyme Levels in Addition To Routine Liver Biochemical Parameters in Sheep With Chronic Fascioliasis
(Univ Federal Santa Maria, 2022) Altug, Nuri; Basbugan, Yildiray; Yuksek, Nazmi
This study assessed changes in the levels of adenosine deaminase (ADA) and its isoenzymes in addition to routine liver biochemical parameters in sheep with fascioliasis. The study was conducted on 35 Akkaraman sheep. Of these, 25 sheep were diagnosed with fascioliasis based on anamnesis and clinical signs, and had endoparasites based on parasitological examinations (Fasciola-infected group). The remaining 10 sheep that were sampled from a single healthy herd (same flock) different from the infected group did not have any clinical signs or endoparasites (control group). Total protein (TP), albumin (ALB), and globulin (GLB) levels gradually increased on days after treatment compared to the values measured before treatment; the increases were statistically significant on all days for TP levels but only on day 14 after treatment for GLB levels (P < 0.05). Although, the ALB levels did not increase significantly on days after treatment, the ALB level and ALB/GLB ratio on days 7 and 14 after treatment were still lower than the values of day 21 after treatment and control group (P < 0.05). Total bilirubin (T-Bil) and direct bilirubin (D-Bil) levels on days 14 and 21 were significantly lower than that of day 0 (before treatment) and day 7 after treatment (P < 0.05). These results indicated that the increase in adenosine deaminase (ADA) and ADA1 levels may be due to possible concomitant infection of Fasciola larvae (in the parenchyma) and adults (in the bile duct).
Comparison of Enalapril, Candesartan and Intralesional Triamcinolone in Reducing Hypertrophic Scar Development: an Experimental Study
(Springer, 2018) Demir, Canser Yilmaz; Ersoz, Muhammet Eren; Erten, Remzi; Kocak, Omer Faruk; Sultanoglu, Yilmaz; Basbugan, Yildiray
The purpose of this study was to compare the effects of oral enalapril, an angiotensin-converting enzyme inhibitor (ACE-I), oral candesartan, an angiotensin receptor blocker (ARB), and intralesional corticosteroid treatments in reducing scar formation. Twenty male rabbits were divided into five study groups: A (sham), B (control), C (ACE-I), D (ARB) and E (intralesional corticosteroid). The rabbit ear hypertrophic scar model was used. The hypertrophic scars were photographed and analyzed with the program ImageJ quantitatively to determine the degree of collagen fibers. The scar elevation index (SEI) was calculated at the end of the 40th day. Tissue samples were stained with hematoxylin and eosin and Masson's trichrome and examined under light microscopy for the determination of fibroblast number, epithelization, vascularization, inflammation and fibrosis. The SEI was the highest in the control group with the highest number of fibroblasts under the epithelium. In the steroid group, the SEI was significantly lower than both the ACE-I (p: 0.02) and ARB (p: 0.001) groups. The density of type 1 collagen fibers was the lowest in the control group, whereas type 3 collagen fibers were highest in that group. The ACE-I and ARB groups were similar regarding densities of type 1 and type 3 collagen fibers. The density of type 1 collagen fibers was the highest in the steroid group, whereas the density of type 3 collagen fibers was the lowest in that group. Enalapril, candesartan and intralesional steroid therapies were all effective in reducing scar tissue development; however, enalapril and steroid groups revealed better results. This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.
Detection of Cryptosporidium Spp. in Calves Through Nested Pcr and Kinyoun's Acid-Fast Methods in Van, Turkey
(Health & Environment Assoc, 2020) Ayan, Adnan; Kilinc, Ozlem Orunc; Yuksek, Nazmi; Basbugan, Yildiray
Cryptosporidium is a protozoon of zoonotic importance causing infections with diarrhoea and other clinical symptoms in man, domestic, and wild animals. The present study was conducted to determine the prevalence of cryptosporidiosis in diarrhoeic calves which were up to 1 month old in Van, Turkey, using Kinyoun's acid-fast and Nested PCR methods. In this study A total of 164 diarrhoeic calves faecal samples were collected from diarrheic calves at different farms located in Van, Turkey during January 2019 to September 2019. The faecal samples stained with Kinyoun's acid-fast method then examined with microscope. DNAs were extracted from all the faecal samples using RTA stool DNA isolation kit. In order to amplify the SSU rRNA gene region of the DNAs, appropriate primers were used in the Nested PCR. According to the results, In the microscopic examination, Cryptosporidium spp. oocyst were identified in 61 out of 164 diarrhoeic calves faecal samples (37.19%). Cryptosporidium spp. specific bands of 826-864 bp in size were obtained from 72 out of 164 diarrhoeic calves faecal samples (43.9%). To the best of our knowledge, no other previous study is available with concern to molecular investigation of the prevalence of Cryptosporidium in calves around Van, Turkey. With this study, it was revealed that cryptosporidiosis is an important agent in calf diarrhoea, and measures should be taken in this regard.
Determination of Serum Cardiac Biomarkers and Plasma D-Dimer Levels in Anemic Sheep With Babesiosis
(Tubitak Scientific & Technological Research Council Turkey, 2015) Orunc Kilinc, Ozlem; Goz, Yasar; Yuksek, Nazmi; Basbugan, Yildiray; Yilmaz, Ali Bilgin; Atas, Ahmet Duran
In this study, the cardiac effects of anemia and venous thromboembolism (VTE), reportedly caused by the hemolyzed erythrocytes occurring in sheep with babesiosis, were investigated using cardiac markers and D-dimer (DD). The study included 34 sheep: 24 infected Akkaraman sheep (1-3 years old, diagnosed with babesiosis based on clinical and laboratory findings) and a control group of 10 noninfected healthy sheep of the same breed and age. Hematocrit (Hct) levels were measured in blood obtained from both groups. Cardiac troponin I (cTnI), creatine kinase-MB (CK-MB), and aspartate aminotransferase (AST) levels were measured in serum samples. In addition, the levels of DD were also measured in plasma samples. Sheep with babesiosis were subsequently divided into 3 subgroups according to their Hct levels, which ranged from 13.2% to 16.3% in the first group (n = 8), 20.1% to 25.6% in the second group (n = 8), and 27.4% to 30.3% in the third group (n = 8). Evaluations of the measurements of cTnI, CK-MB, and AST levels indicated statistically significant differences between infected and healthy sheep. Statistically significant differences were not found for DD levels between the 2 groups.
Diagnostic and Prognostic Value of Procalcitonin (Pct), C Reactive Protein (Crp), Nitric Oxide (No) Levels, and Adenosine Deaminase (Ada) Activity in Sheep With Natural Babesiosis Before and After Treatment
(Tubitak Scientific & Technological Research Council Turkey, 2018) Arslan, Sezai; Altug, Nuri; Muz, Mustafa Necati; Yuksek, Nazmi; Basbugan, Yildiray; Oruk Kilic, Ozlem
This study was carried out to reveal the importance of procalcitonin, C reactive protein, nitric oxide levels, and adenosine deaminase activity in the diagnosis and prognosis of the disease in naturally infected sheep with Babesia ovis. Thirty sheep diagnosed clinically and parasitologically as having Babesia ovis were allocated to 2 groups. The first group was treated only with imidocarp dipropionate and the second group with imidocarp dipropionate and flunixine meglumin. On the seventh day after treatment, blood samples were collected again from the sheep in the babesiosis-infected group and the treatment responses were assessed. Serum PCT (1.72 +/- 0.34 ng/mL, P < 0.01), CRP (101.42 +/- 11.73 mu g/mL, P < 0.001), NO (15.77 +/- 2.75 mu mol/L, P < 0.01), and ADA (13.92 +/- 0.88 IU/L, P < 0.01) were higher in sheep with babesiosis than in the healthy sheep (0.49 +/- 0.04 ng/mL, 49.46 +/- 4.57 mu g/mL, 8.15 +/- 0.63 mu mol/L, 9.34 +/- 1.19 IU/L, respectively). When PCT, CRP, NO, and ADA before treatment and after treatment in the infected sheep were compared, the levels of these parameters except for ADA in the second group were determined to have statistically decreased after the treatment. As a result, it has been concluded that the measurements of PCT, CRP, NO, and ADA in sheep with babesiosis may be useful for the diagnosis and prognosis of the disease when assessed in association with clinical examination.
Effect of Meloxicam and Flunixin Meglumine on Some Kidney Parameters in Geriatric Male Rats
(Univ Zulia, Facultad Ciencias veterinarias, 2024) Komuroglu, Ahmet Ufuk; Basbugan, Yildiray; Yuksek, Nazmi; Altug, Nuri; Ozdek, Ugur; Dede, Semiha
Advanced age, called geriatrics, negatively affects the aging- related physiological changes on bodysystems. non-steroidal anti- inflammatory drugs (NSAIDs) used in geriatric pets or humans have negative effects on the kidneys. For this purpose, , the effects of Meloxicam and Flunixin Meglumine, which are NSAIDs frequently used in Veterinary Medicine, on the liver and kidney structure and function in geriatric male rats were evaluated. Twenty-four male geriatric rats (30-36 month old) and twenty-four 3-month-old young male Wistar albino rats were used in the study. Six groups were created, with eight rats in each group: young control (YC), young Meloxicam (YM), young Flunixin Meglubine (YFM), geriatric control (GC), geriatric meloxicam (GM), geriatric Flunixin Meglubine (GFM). Control groups (YC and GC) received an intraperitoneal injection of saline using the same volume as in the othergroups. Meloxicam was administered at 5.8 mgkg-1to-1 to the YM and GM groups, and Flunixin Meglumine at 2.5 mgkg-1-1 intraperitoneally to the YFM and GFM groups once a day for 5 days. Neutrophil Gelatinase-Associated Lipocalin (NGAL), Cystatin C (Cyc-c), Kidney Injury Molecule-1 (KIM-1), Interleukin-18 (IL-18), Urea, Creatinine (Crea), Albumin (Alb), and Total Protein (TP) levels, were determined in sera and urine samples. Serum NGAL, Cys-C, and KIM-1 levels in the GC group were found to be significantly higher than those in the YC group (P<0.05). P <0.05). Administration of both NSAIDs caused an increase in serum Cyc-c and NGAL levels in both young and geriatric rats (P<0.05). P <0.05). Since both Meloxicam and Flunixin Meglumine administration caused an increase in NGAL and Cys-c levels in young and geriatric rats, adjusting the drug dose and frequency of administration by evaluating the pretreatment renal function should be considered as a preventive measure.
The Effect of Pde5 Inhibitors on Bone and Oxidative Damage in Ovariectomy-Induced Osteoporosis
(Sage Publications Ltd, 2017) Alp, Hamit H.; Huyut, Zubeyir; Yildirim, Serkan; Basbugan, Yildiray; Ediz, Levent; Sekeroglu, Mehmet R.
Osteoporosis is a major public health problem associated with many factors, and it affects more than 50% of women over 50 years old. In the current study, our purpose was to investigate the effects of phosphodiestarase-5 inhibitors on osteoporosis via the nitric oxide/3',5'-cyclic guanosine monophosphate/protein kinase G signalling pathway. A total of 50 female albino Wistar rats were separated into five groups. The first group was appointed as the healthy control group with no ovariectomy. All animals in the other groups underwent a bilateral ovariectomy. Six months after the ovariectomy, vardenafil, udenafil and tadalafil were given to the third, fourth and fifth groups, respectively, but were not administered to the positive control group (10 mg/kg per day for two months). The bone mineral density values were determined using a densitometry apparatus for all groups pre- and post-ovariectomy as well as after treatment. The levels of nitric oxide, endothelial nitric oxidesynthase, asymmetric dimethylarginine, 3',5'-cyclic guanosine monophosphate, protein kinase G, phosphodiestarase-5, pyridinoline, deoxypyridinoline, carboxyterminal telopeptide fragments and plasma carboxy terminal propeptide of type I collagen were determined using an enzyme linked immunosorbent assay. The levels of malondialdehyde, 8-hydroxy-2-deoxy guanosine, deoxyguanosine and coenzyme Q10 were determined by a high-performance liquid chromatography assay. Additionally, the right femoral trabecular bone density and the epiphyseal plate were measured in all groups. Angiogenesis was histologically observed in the bone tissue. In addition, we determined that the inhibitors may have caused a positive impact on the increased bone mass density and reduction of bone resorption markers. We also observed the positive effects of these inhibitors on oxidative stress. In conclusion, these phosphodiestarase-5 inhibitors increase angiogenesis in bone tissue and improve the re-formation rate of bone in rats with osteoporosis. Chemical compounds studied in this article Udenafil (PubChem CID: 6918523); Tadalafil (PubChem CID: 110635); Vardanafil (PubCham CID: 110634). Impact statement The results in our study appear to establish the osteoporosis model and provide evidence of the positive effects of three separate PDE5 inhibitors (vardenafil, udenafil, and tadalafil). The positive effects of these PDE5 inhibitors are investigated and demonstrated by the bone mass density and bone resorption markers. These effects are associated with significant demonstrated antioxidant activities. Osteoporosis is a significant major public health problem especially in more aged populations. Advances in identifying and understanding new potential therapeutic modalities for this disease are significant. This study provides such an advance.
The Effect of Vitamin E Prophylaxis on Experimental Amikacin-Induced Nephrotoxicity Model
(Oxford Univ Press, 2016) Bulut, Gulay; Ari, Elif; Basbugan, Yildiray; Alp, Hamit Hakan; Bayram, Irfan
Effects of Acute and Chronic Fluoride Administration on Some Kidney Parameters of Rats (cys-C, Kim-1, and Ngal)
(int Soc Fluoride Research, 2021) Komuruglu, Ahmet Ufuk; Basbugan, Yildiray; Arihan, Okan; Arihan, Seda Karaoz
Fluorosis is an important disease both in modern societies as well as in the previous periods. Paleopathological studies reveal that people in ancient periods suffered from fluorosis which causes adverse health effects, especially on the musculoskeletal system as well as soft tissues such as kidneys. In this study, we aimed to investigate the effects of acute and chronic fluoride administration on some kidney markers in rats. Fifty-six Wistar albino rats were divided into 7 groups, 8 in each group. Acute fluoride intoxication was established by administering, in drinking water, 5 ppm (group 2), 15 ppm (group 3), and 50 ppm (group 4) for 7 days. Chronic fluoride intoxication was established by administering 5 ppm (group 5), 15 ppm (group 6), and 50 ppm for 90 days (group 7). Control group (group 1) was given tap water. At the end of the study, the rats were sacrificed under anesthesia and blood samples were taken. The blood was centrifuged and their serums were separated. CYS-c, KIM-1, and NGAL levels were measured by ELISA method, and urea, creatinine, total protein, and albumin levels were measured spectrophotometrically. CYS-c levels were increased in all groups administered fluoride (p>0.05). Similarly all groups had higher levels of NGAL due to fluoride exposure and chronic fluoride 5 mg/L group showed significant increase compared to control (p<0.05). In KIM-1 values, a significant increase occurred in acute fluoride 15 and 50 mg/L (p<0.05). Significant alterations were also observed in creatinine and urea values due to fluoride exposure. Consequently, exposure to fluoride may cause an increase in serum inflammation markers (NGAL, KIM-1) due to differences in dosage and exposure period. Further long term studies, including molecular and histopathological assessments, are needed to elucidate the impact of long term exposure to fluoride on the renal system.
Effects of Injectable Platelet-Rich Fibrin in Experimental Periodontitis in Rats
(Springer, 2021) Aydinyurt, Hacer Sahin; Sancak, Tunahan; Taskin, Cem; Basbugan, Yildiray; Akinci, Levent
Injectable platelet-rich fibrin (i-PRF) is an effective biological material that positively contributes to angiogenesis, wound healing, inflammation, regeneration processes, etc. This research aimed to evaluate the effect of i-PRF in rats with experimental periodontitis. Following the development of ligature-induced periodontitis, 24 Wistar albino rats were divided into three groups. Group-1: scaling and root planing (only-SRP); Group-2: SRP + i-PRF; Group-3: only- i-PRF. Heart blood from six donors was used for the i-PRF application. i-PRF was administered as a subgingival injection in the relevant groups on the 1st, 3rd, and 7th days. The tissues were evaluated histopathologically and immunohistochemically. Also, bone structures were examined using micro-CT. According to the data obtained, no statistically significant difference was observed among the groups in terms of bone resorption, inflammation, bone volume, bone levels (mesial/distal), and IL-1 beta, IFN-r, TNF-alpha, VEGF values (p > 0.05). However, bone mineral density was statistically significantly different among the groups (Group3 > Group2 > Group1) (p < 0.0001). Subgingival injection of only-i-PRF showed promising results in periodontitis treatment but contribution to SRP has not been proved according to this study results. The study results suggested that the i-PRF application was as effective as SRP in reducing bone loss, modulating inflammatory process, and effecting cytokines in experimental periodontitis. The significant effect of i-PRF on bone mineral density was the most remarkable result.
Effects of Thymus Vulgaris L. in Acute and Chronic Epilepsy Models in Rats Induced by Pentylenetetrazole
(Kafkas Univ, veteriner Fakultesi dergisi, 2019) Ozdemir, Hulya; Sagmanligil, Vedat; Erkec, Ozlem Ergul; Oto, Gokhan; Basbugan, Yildiray; Uyar, Hasan
The aim of this study was to investigate the effects of Thymus vulgaris L. (TVL) on oxidative stress, motor coordination and learning/memory in acute and chronic epilepsy models in rats induced by Pentylenetetrazole (PTZ). To this end, 64 male Wistar-albino rats were randomly divided into eight groups with 8 rats each: (1) acute control (AC), (2) acute PTZ (APTZ), (3) acute PTZ + sodium valproate (APTZ+VPA), (4) acute PTZ + TVL (APTZ+TVL), (5) chronic control (CC), (6) PTZ kindling (PTZk), (7) PTZ kindling + VPA (PTZk+VPA) and (8) PTZ kindling + TVL (PTZk+TVL). Seizures were observed for 30 min after each PTZ injection and were scored. Acute PTZ-induced seizures were created by injecting a single convulsive dose of PTZ (60 mg/kg, ip) in acute groups. PTZ kindling was produced by injecting a subconvulsant dose of PTZ (35 mg/kg, ip) every other day, with 14 injections in total. No significant difference was found among the PTZk + VPA, PTZk, and PTZk + TVL groups with regard to seizure scores. No significant difference was found among all the 8 groups in the learning/memory tests conducted using the Morris Water Maze (MWM) test and the motor activity tests conducted using the rotarod test (P>0.05). The analysis of total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) indicated that the administration of PTZ decreased the antioxidant capacity and increased the oxidant capacity. Moreover, the TVL administration established the oxidant/antioxidant balance, particularly in the chronic groups. Further studies are needed to investigate whether high doses of TVL have an effect on PTZ-induced seizure scores.
Evaluation of Oxidant, Antioxidant, Interleukin and Biochemical Parameters in Sheep With Cystic Echinococcosis and Dicrocoeliosis
(Polish Soc veterinary Sciences Editorial office, 2025) Ertas, Fatma; Komuroglu, Ahmet Ufuk; Kilinc, Ozlem Orunc; Basbugan, Yildiray; Yuksek, Nazmi
Aim: Endoparasitic infections have long posed a significant threat to the sheep industry, leading to substantial economic losses. Among these parasites, Dicrocoelium dendriticum and, particularly, Echinococcus granulosus are globally important zoonotic parasites. This study aimed to investigate the levels of oxidative stress markers, antioxidant parameters, interleukins, and biochemical indicators, as well as their correlations, in sheep affected by cystic echinococcosis and dicrocoeliosis. Methods: In this study, various biochemical and immune-related parameters were assessed. Serum samples collected from infected sheep were analyzed using ELISA to measure interleukin-18 (IL-18), interleukin-10 (IL-10), interleukin-6 (IL-6), interleukin-4 (IL-4), NGAL, neopterin, myeloperoxidase, SOD, GPX, catalase, GSH, and MDA. Additionally, spectrometric analysis was performed to determine the levels of AST, ALT, GGT, albumin, globulin, total protein, ALP, and iron. Results: Serum MDA levels were found to be significantly elevated in infected sheep compared to the healthy group. In contrast, serum SOD and GSH levels in infected sheep were significantly lower than those in healthy sheep. Additionally, serum AST and ALT activities were markedly higher in the infected group. Interleukin levels showed no significant differences between the two groups. Overall, markers of liver function and oxidative stress were notably increased in infected sheep. Conclusion: It was concluded that oxidative stress and liver damage occurred in the infected group, and their immune system was actively involved in the response. This study not only adds to academic knowledge, but should also help veterinarians and breeders working in the field to make more accurate decisions. Thus it is expected to contribute directly to herd health, animal welfare, economic efficiency, and public health. Correlation data may also be useful in guiding the treatment process.
Examination of Sheep in Which Injection of Yeldif® Induced Sudden Death
(Polish Soc veterinary Sciences Editorial office, 2017) Uyar, Ahmet; Yener, Zabit; Basbugan, Yildiray; Keles, Omer Faruk; Yaman, Turan
Two breeders from the Baskale district of the Van province and the Hizan district of the Bitlis province reported deaths of their lambs one day after injection of a commercial preparation containing a combination of Se and Vit E (Yeldif (R)). Purulent-hemorrhagic-necrotic myositis was detected at the injection site during necropsy of the lambs (2 animals) brought to the Pathology Department of our Faculty by these breeders. This study was conducted to determine whether these post-injection deaths occurred as a result of the drug used or due to a faulty application of the medicine by the breeders. The trial period for this study was 1 week. Eighteen 2-month-old lambs were used in this study. The lambs were divided into three groups (control and 2 study groups) of 6 lambs each. An injection of 1 ml (recommended dose) of the product purchased in the market from the same batch as the one that had caused the deaths was administered I.M. to lambs in the control group. The medicine reported to have caused the deaths by the breeders was administered to the first study group (A) at 1 ml I.M. and to the second study group (B) at 3 ml I.M. (three times the recommended dose). The lambs were bled for biochemical analysis before the administration, on the first day after the administration, and at the end of the trial. Necropsies were performed on dead animals during the trial and on all animals at the end of the trial. At the end of the trial, no clinical-pathological findings were noted in lambs of the control group and group A. However, in all lambs of group B, emphysematous gangrene was detected at the injection site. Cl. chauvoei was isolated and identified in bacteriological and cultural examinations. It was concluded that bacterial contamination occurred as a result of incorrect drug administration by the breeders and injection of a high dose of the contaminated drug.
Experimental Evaluation of Timing and Preference of Surgical Intervention for Crush Syndrome in Disaster Scenarios: Fasciotomy or Amputation? a Rat Model Study
(BMC, 2025) Kaya, Sehmuz; Unal, Yunus Can; Guven, Necip; Dundar, Abdulrahim; Keles, Omer Faruk; Basbugan, Yildiray
Background Crush syndrome is a severe condition caused by the systemic effects of rhabdomyolysis due to prolonged muscle compression. Common in disasters like earthquakes, it poses life-threatening risks, including acute renal failure, hyperkalemia, and metabolic acidosis. Although surgical interventions such as fasciotomy and amputation are critical in its management, the optimal timing and criteria remain unclear. This study investigates the impact of surgical intervention timing on crush syndrome outcomes, providing guidance through the first experimental rat model evaluating fasciotomy and amputation post-injury. Methods Forty-eight Wistar albino rats were divided into six groups. Rhabdomyolysis was induced experimentally, followed by amputation or fasciotomy at 0, 12, or 24 h. The control group underwent rhabdomyolysis induction without surgery. Weekly urine samples were collected during the study, and blood, muscle, and kidney tissues were examined biochemically and histopathologically at the experiment's end. Data analysis focused on the systemic and tissue-specific effects of intervention timing. Results Early intervention groups (0 h) demonstrated minimal muscle inflammation and necrosis, preserved muscle fiber arrangement, and more pronounced regeneration. Late interventions (12 and 24 h) were associated with intense inflammation, necrosis, edema, and hemorrhage in muscle tissue. Immediate amputation (0 h) limited toxic metabolite effects, reducing kidney inflammation and damage. Late interventions showed increased interstitial nephritis and inflammatory cell infiltration in kidney tissue. Biochemical analyses revealed that urinary myoglobin levels decreased and renal function was preserved in the early intervention groups. Conclusions The timing of surgical intervention is critical in managing crush syndrome. Early amputation and fasciotomy minimized necrosis and inflammation in muscle tissue, supported tissue regeneration, and reduced systemic complications by preventing toxic metabolite accumulation in the kidneys. Early amputation particularly showed superior renal protection and lower systemic complication risks compared to late interventions. These findings highlight the importance of timely surgical action and provide valuable insights for developing effective treatment strategies in disaster settings. However, the descriptive nature of the study and the fact that the data obtained from the experimental model cannot be directly applied to clinical practice should be taken into account. Therefore, the findings should be supported by future clinical trials.
Influence of Some Β-Lactam Drugs on Selected Antioxidant Enzyme and Lipid Peroxidation Levels in Different Rat Tissues
(Taylor & Francis Ltd, 2020) Turkan, Fikret; Huyut, Zubeyir; Basbugan, Yildiray; Gulcin, Ilhami
Antioxidant enzymes play an important role in body defense and free radical removal. Cephalosporins are beta-lactam antibiotics. In this work, the effects of cefazolin, cefuroxime and cefoperazone which are cephalosporins on some selected antioxidant enzyme and levels of malondialdehyde (MDA) as lipid peroxidation product were investigated in kidney, liver, and brain tissues of albino female rats. Ninety-six albino rats were randomly divided into 16 groups of equal number (n = 6). 50 mg/kg cefazolin, 25 mg/kg cefuroxime, and 100 mg/kg cefoperazone were injected intraperitoneally to the groups (5th-8th and 9th-12th, and 13th-16th groups), respectively. The changes in glutathione reductase (GR), glutathione S-transferase (GST), superoxide dismutase (SOD), peroxidase (POD), and glutathione peroxidase (GSH-Px) levels were studied in each time point group and a time-dependent manner (at the 1st, 3rd, 5th and 7th hour). In addition, MDA levels were examined in all the tissues. The drugs evaluated in this study had different effects on the same enzyme in different tissues depending on time. MDA levels especially in cefazolin and cefoperazone experiments were lower in all the tissues; however, MDA levels were higher in brain and kidney tissues in the cefuroxime groups in a time-dependent manner (p < 0.05). These results revealed the complex effects of the tested drugs on different tissues at different time points. Therefore, the dose and use of these drugs should be adjusted correctly.
Investigation of the Effect of the Efficiency of Noise at Different Intensities on the Dna of the Newborns
(Medknow Publications & Media Pvt Ltd, 2016) Ceylan, Nesrin; Kaba, Sultan; Karaman, Kamuran; Celiker, Metin; Basbugan, Yildiray; Demir, Nihat
Hearing loss can occur in newborns exposed to high-level noise; noise exposure can cause more physiological stress and can lead to DNA damage. This study was designed to determine DNA damage in newborn rats exposed to sound at different concentrations. For this purpose, 28 newborn (3-6 days old) rats were divided into four groups of 7 rats in each group (Control and Groups of 40 decibel (dB), 70 dB, and 110 dB]. In the experimental groups, 40 dB, 70 dB, and 110 dB (7.5-15 kHz) of sound was applied to the experimental groups for 30 min a day for 7 days. DNA damage levels in the serums obtained from this study were determined by the enzyme-linked immunosorbent assay (ELISA) method. According to this, it was determined that DNA damage in the group exposed to 110 dB showed a statistically significant increase (P < 0.05) compared to the compared to the control, 40 dB, and 70 dB groups. Related to the subject, it was concluded that DNA damage may occur in newborns exposed to 110 dB or higher sound in neonatal units, wards, and home environments with newborn babies. Mothers should be warned about this situation and noise should be kept under 110 dB volume in the environments with the newborns.
Investigation of the Impact of Antiparasitic Drug Moxidectin on the Rewarding Effects of Alcohol
(Aepress Sro, 2022) Ekici, Abdurrahman; Gurbuz, Esra; Berkoz, Mehmet; Turkmen, Omer; Basbugan, Yildiray; Yunusoglu, Oruc
Alcohol addiction or alcoholism constitutes a significant risk factor worldwide for morbidity and mortality. Moxidectin is a recently approved anthelmintic drug, which also activates the gamma-aminobutyric acid receptors. The objective of the present study was to examine the impact of moxidectin on rewarding effects of ethanol in the conditioned place preference (CPP) model in mice. In separate experiments, mice were administered intraperitoneal (i.p.) injections of moxidectin (5 or 10 mg/kg) before a) acquisition of alcohol-induced CPP, b) each extinction session, and c) alcohol-induced reinstatement of CPP. The present experiments provide consistent data about ethanol place preference in mice (2 g/kg, i.p.), with mice in all tests spending significantly more time on the ethanol-paired side. The acquisition of the CPP response to ethanol was prevented by the administration of moxidectin at a dose of 10 mg/kg. Additionally, moxidectin treatment accelerated the extinction of ethanol CPP when given repeatedly during the extinction phase. Ethanol-induced reinstatement of CPP following an extinction phase was inhibited by moxidectin. Ethanol alone and co-administration with moxidectin did not change locomotor activity and motor coordination. In conclusion, we suggest that moxidectin may be a promising therapeutic candidate for prevention of ethanol-induced addiction and relapse as well as detoxification.
An Investigation on Serum Troponin Concentration in Healthy Ruminants
(Kafkas Univ, veteriner Fakultesi dergisi, 2010) Basbugan, Yildiray; Agaoglu, Zahid; Yuksek, Nazmi
The present study aimed to investigate the cardiac troponin (cTn I and cTn T) levels in healthy ruminants. The study involved 30 cattle (15 male, 15 female), 30 Akkaraman sheep (15 male, 15 female) and 30 goats (15 male, 15 female) and all animals were clinically healthy. The study animals were grouped as 0-6 months, 6-12 months and 12 months and over. Blood samples were obtained from jugular vein and cardiac troponin I and T levels, and biochemical parameters (CK, CK-MB, AST and LDH) were measured. Troponin T values were found as negative in all three species. Troponin I values were 0-0.23 ng/ml (mean 0.18 ng/ml) in cattle, 0-0.21 ng/ml (mean 0.15 ng/ml) in sheep and 0-0.24 ng/ml (mean 0.20 ng/ml) in goats. It was also found that the values were in declared limits for cattle and sheep. Although CK, CK-MB, AST and LDH values were in normal ranges AST levels were higher in cattle and sheep of 0-6 months of age and, LDH levels were higher in sheep and goat at 6-12 months of age. In conclusion, obtained troponin values in this study could be values for healthy ruminants.
The Levels of Trace Elements and Selected Vitamins in Goats With Chronic Fluorosis
(Tubitak Scientific & Technological Research Council Turkey, 2013) Altug, Nuri; Arslan, Sezai; Yuksek, Nazmi; Keles, Ihsan; Yoruk, Ibrahim Hakki; Basbugan, Yildiray; Aytekin, Ismail
This study was conducted to investigate the effects of chronic fluorosis on trace elements and vitamin levels in goats. Thirty-three goats exhibiting clinical signs of chronic fluorosis and 10 healthy goats from the same region were used. Animals with chronic fluorosis were divided into 2 groups according to their age as 1-3 years old and 4-6 years old. Blood samples were collected from all goats. Fluoride concentrations were determined with an ionometer. Na, K, and Cl levels were measured with ion-selective equipment and serum mineral substance levels were obtained by atomic absorption spectrophotometer. Ca, Mg, and P levels were determined by biochemistry analyzer. Serum vitamin concentrations were determined by liquid chromatography. The levels of serum copper, iron, manganese, and nickel decreased in goats with chronic fluorosis, whereas other trace elements did not change. Among vitamins, levels of serum a-tocopherol were increased, whereas retinol and vitamin D3 levels were not changed significantly differently between the fluorosis and control groups. In evaluation of goats with fluorosis according to age group, copper levels in the age group of 1-3 years and iron levels in the age group of 4-6 years were found to be lower than in the other age groups. Other minerals and all vitamin parameters were not different according to age. The results of the present study clearly showed that serum copper, iron, manganese, and nickel levels were reduced in goats with chronic fluorosis, and so goats with fluorosis should receive support in terms of these concerned trace elements.