Browsing by Author "Bastan, A"

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Cryopreservation of Immature Bovine Oocytes by Vitrification in Straws
(Elsevier, 2006) Cetin, Y; Bastan, A
The aim of this study was to cryopreserve by vitrification by ethylene glycol (EG) and dimethyl sulfoxide (DMSO) immature bovine oocytes in straws and to investigate the effects of vitrification on post-thaw oocyte maturation. A total of 575 cumulus oocyte complexes were obtained by follicle aspiration from 238 ovaries of cows slaughtered at a local abattoir. Following selection, oocytes with compacted cumulus cells and evenly granulated ooplasm were vitrified using one of the three different solutions with a nonvitrified group served as control. The first step vitrification solution contained 20% EG while the second step solution contained 40% EG + 1 M sucrose in a basic media used in group EG. Oocytes were matured in N-2-hidroxyethyl piperazine-N-2-ethanosulfonic acid (HEPES) buffered tissue culture medium (TCM) 199 for 24h at 39 degrees C in a humidified atmosphere of 5% CO2 in air. Oocytes were fixed following evaluation for polar body formation, stained with Giemsa solution and nuclear maturation was examined. The numbers of oocytes which were observed at Metaphase II (MII) stage were 41 (34.1%), 17 (14.9%) 29 (20.7%) and 78 (79.6%) in groups EG, DMSO, Mix and Control, respectively. Maturation rate distribution in group Mix was not statistically different when compared to maturation rate distributions in groups EG and DMSO (p > 0.05). Differences between other groups were significant (p < 0.001). However, better results were obtained in EG group compared to DMSO and mix groups. Maturation rates were lower in all treatment groups than the control group. The lowest maturation result was obtained in DMSO group. Maturation rate in group Mix was between maturation rates of EG and DMSO groups.
Determination of the Relationship Between Beta-Carotene and Vitamin a and Functional Structures on Ovaries in Bitches
(Scientific Technical Research Council Turkey, 2003) Bastan, A; Güngör, Ö; Çetin, Y; Salmanoglu, B
The purpose of this study was to evaluate the effects of beta-carotene and vitamin-A blood levels on follicular development (F) and corpus luteum (CL) numbers in bitches. Thirty-four randomly selected dogs brought to the Ankara University, Veterinary Faculty, Gynacology Department Clinic for ovariohysterectomy were used. The sera (separated from the dogs' blood samples) before surgery were sent to the laboratory to determine beta-carotene and vitamin-A levels. The numbers of functional structures (F and CL) on the ovaries, obtained after surgery, were determined. Bitches without functional structures on the ovaries were not evaluated in this study. Bitches with functional structures on the ovaries were divided into two main groups. One to 3 CL were detected on the ovaries of three bitches (Group 1) and more than three on 17 bitches (Group 11). The mean levels of beta-carotene and vitamin-A in groups I and 11 were 50.0 +/- 8.6 and 48.1 +/- 5.3, and 6.4 +/- 2.1 and 4.0 +/- 0.4 mug/dl, respectively. One to 3 F were detected in five bitches (Group 111) and more than three on nine bitches (Group IV). The mean levels of P-carotene and vitamin-A in groups III and IV were 47.6 +/- 10.7 and 60.7 +/- 6.5, and 3.4 +/- 0.8 and 5.5 +/- 0.8 mug/dl, respectively. In conclusion, although a relation between vitamin-A and follicle and CL numbers has not been found, beta-carotene seems to have a positive correlation with follicle numbers in bitches. More detailed research should be carried out in order to determine the effects of beta-carotene on CL numbers.
Endometrial Cytology Findings at Different Stages of Sexual Cycle, Pregnancy and Pyometra in Bitches
(Scientific Technical Research Council Turkey, 2003) Bastan, A; Çetin, Y; Güngör, Ö; Basaran, DA
The purpose of this study was to investigate cellular changes of the uterus during pregnancy, pyometra and different phases of the sexual cycle in dogs using endometrial cytology. We used 30 uteruses from bitches of different ages and breeds and at heterogeneous stages of the sexual cycle (Group 1 proestrus n = 5, Group 2 estrus n = 5, Group 3 diestrus n = 5, and Group 4 anestrus n = 5) as well as animals with open cervix pyometra (Group 5 n = 5), and that were pregnant (Group 6 n = 5). The sexual cycle stage of the bitches was identified by vaginal cytology prior to ovariohysterectomy. The groups were selected from a large number of samples following vaginal cytology and ultrasonographic scanning. Blood samples (10 ml) were collected from the bitches by puncturing the v. cephalica antebrachi before surgery. The endometrium was revealed by longitudinal dissection of uterine horns after laparotomy. Uterine wall swabs were obtained from the mid part of one horn by rotating the cotton swabs on the endometrial surface. Cotton swabs were smeared over microscopic slides and the samples were dyed using the Papanicolau technique. The neutrophils, erythrocytes, bacteria and endometrial epithelial cells were evaluated for endometrial cytology. Cell scores were given that ranged from 1 to 4 according to frequency. In this study, variations in the type and density of cells according to stages of the sexual cycle, as well as pregnancy and pyometra cases, were observed. In the proestrus stage, an excessive number of erythrocytes, and a few neutrophils, endometrial epithelial cells and bacteria were found. In the estrus stage, a few neutrophils and erythrocytes and a moderate density of bacteria were observed, while in the diestrus stage, a few endometrial epithelial cells and neutrophils were noted. In the anestrus stage, a moderate density of degenerated endometrial epithelial cells, neutrophils and bacteria were observed. During pregnancy, a few epithelial cells and neutrophils were found. In pyometra cases, dense bacteria degenerated epithelial cells and neutrophils were identified. The number of neutrophils was lower in the progesterone dominated phase than in the estrus stage. In conclusion, endometrial cytology can be used to determine physiological and pathological changes in the endometrium and that when transcervical cannulation techniques are fully developed they will constitute a practical. cheap and reliable diagnostic tool to identify uterine disorders.