Browsing by Author "Bayram, U.F."

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Antiproliferative Effects of Pyranopyrazole Analogs: In Vitro and Molecular Docking Analysis
(KeAi Communications Co., 2026) Nalci, K.A.; Cetin, A.; Bildirici, I.; Bayram, U.F.
Background Glioblastoma multiforme is the most aggressive primary brain tumor in adults, and current multimodal therapies provide only limited survival benefit. There is an urgent need for new small-molecule scaffolds with improved anticancer activity against glioblastoma. Objective To evaluate the antiproliferative effects of pyranopyrazole analogs on human U-87 MG glioblastoma cells and to explore their potential molecular interactions with a glioblastoma-related protein target by molecular docking. Methods A series of pyranopyrazole analogs ( 12a – o ) was synthesized using a previously reported green-chemistry protocol. U-87 MG cells were treated with increasing concentrations of the analogs for 24, 48, and 72 h, and cell viability was measured by a colorimetric tetrazolium-based assay. Half-maximal inhibitory concentration values were determined for the most active analogs and compared with those of a reference chemotherapeutic agent. Molecular docking was performed with the 6c1c protein structure to assess binding energies and interaction profiles. Results Several pyranopyrazole analogs produced dose- and time-dependent cytotoxicity in U-87 MG cells. Analog 12 g showed the strongest antiproliferative effect and lower half-maximal inhibitory concentration values than the reference agent at earlier time points, whereas 12 h , 12o , and 12i displayed moderate activity. Docking analysis indicated favorable binding of active analogs to the 6c1c active site, with multiple hydrogen-bond and π-type interactions involving key residues. Conclusion Pyranopyrazole analogs, particularly 12 g , exhibit promising antiproliferative activity against glioblastoma cells in vitro, supported by compatible molecular docking findings. These compounds may represent useful lead structures for the development of new chemotherapeutic options for glioblastoma. © 2026 The Authors.
Neuroprotective Effects of Salvia Splendens Extracts Against Rotenone-Induced Oxidative Stress and Cell Death in SH-SY5Y Cells
(Yuzuncu Yil Universitesi Tip Fakultesi, 2026) Nalci, K.A.; Dervişoğlu, G.; Özdemir, F.A.; Yilmaz, M.E.; Yöndeş, S.; Güneş, M.U.; Bayram, U.F.
Numerous studies in literature indicate that rotenone (ROT) has a cytotoxic effect on humans or animals, and it causes a neurodegenerative disease as Parkinson's Disease (PD). Our study showed that ROT can trigger oxidative damage and cause neuron cell loss in SH-SY5Y cells, an in vitro model of dopaminergic neuronal cells. Salvia splendens (S. splendens) can exhibit antioxidant, anti-inflammatory, and cholinergic effects on neuronal cells thanks to the phenolic compounds it contains. This study focused on investigating the neuroprotective impacts of ethanol extracts prepared from aerial and root parts of S. splendens (AE and RE, respectively) against ROT-induced oxidative stress and neuron cell loss in SH-SY5Y cells. In the current study, ROT dose dependently increased neuron cell loss in SH-SY5Y cells, and the IC50 value of ROT was determined as 40 µM. The optimum concentrations of AE and RE, which showed the maximum protective effect on SH-SY5Y viability, were determined as 195.3125 ng/mL in the presence of 40 µM ROT. AE and RE, at a specified concentration of 195.3125 ng/mL, in the presence of 40 µM ROT significantly increased the total antioxidant status (TAS) and did not create a profound difference in the total oxidant status (TOS). Additionally, AE and RE significantly decreased the oxidative stress index (OSI) value, as evidence of total cellular oxidative stress, compared to the ROT group in the presence of ROT. In summary, AE and RE obtained from S. splendens protect the SH-SY5Y cells from ROT-induced damage through reducing oxidative stress and preventing neuronal cell loss. © 2026, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.