Browsing by Author "Bilge-Dagalp, Seval"

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First Genetic Characterization of Equine Adenovirus Type 1 (eadv-1) in Turkey
(Elsevier Sci Ltd, 2012) Ataseven, Veysel Soydal; Oguzoglu, Tuba Cigdem; Basaran-Karapinar, Zeynep; Bilge-Dagalp, Seval
Equine adenovirus type 1 (EAdV-1) is a cause of repiratory tract infection in equids. In present study for the first time in Turkey, the prevalence of EAdV-1 in nasal swab samples obtained from horses showing respiratory symptoms was investigated by polymerase chain reaction (PCR), and molecular characterization of the hexon gene detected in the Turkish (TR) strain was performed. Overall, the prevalence of EAdV-1 was found low (1.4%) as indicated by a positive PCR reaction from the nasal swab extracts tested. Phylogenetic analysis based on the partial sequences of the hexon gene of a TR-EAdV-1 strain with those of previously isolated AdVs from different mammals and an EAdV-1 M1 strain showed that the EAdV-1 strains were placed into a unique cluster. Although the TR-EAdV-1 strain was closely related to CAV-1, CAV-2 and bat adenovirus reference strains, larger-scale studies are necessary to better understand the molecular epidemiology and population structure of EAdV-1 in Turkey. (C) 2011 Elsevier Ltd. All rights reserved.
Seroepidemiological Studies of Equine Herpesviruses 1 (ehv-1) and 4 (ehv-4) Infections in Working Horses From the Eastern Turkey
(Ankara Univ Press, 2010) Ataseven, Veysel Soydal; Bilge-Dagalp, Seval; Basaran, Zeynep; Keskin, Siddik
The objective of this research was to determine the presence of equine herpesvirus types 1 (EHV-1) and 4 (EHV-4) antibodies in local horses from five provinces in the East Anatolia region of Turkey. Blood samples were collected from 405 non-vaccinated horses living in Van, Bitlis, Mu, Erzurum, and Igdir, and were analyzed via a commercial enzyme-linked immunosorbent assay (ELISA). EHV-1- and EHV-4-specific antibodies were detected in 94 (23.2%) and 316 (78.0%) of the 405 tested sera, respectively. The seropositivity percentages for EHV-1-specific antibodies by province were as follows: 29.5% in Van, 6.1% in Bitlis, 5.1% in Mus 24.0% in Igdir, and 20.6% in Erzurum. EHV-4-specific antibodies were detected at levels of 87.2% in Van, 61.2% in Bitlis, 56.4% in Mus 84.0% in Igdir, and 52.9% in Erzurum. The present investigation demonstrated that EHV-1 and EHV-4 infections were more prevalent in the indigenous horse population from five provinces in the East Anatolia region of Turkey. Also, this data on the high seropositivity of both infections in two provinces (Van and Igdir) along the eastern border of Turkey may be also serve as a risk indicator for future introductions of some transboundary diseases via border trade.