Browsing by Author "Bingol, Zeynebe"

Now showing 1 - 8 of 8

Analysis of Phenolic Compounds by Lc-Hrms and Determination of Antioxidant and Enzyme Inhibitory Properties of Verbascum Speciousum Schrad
(Acg Publications, 2023) Kiziltas, Hatice; Bingol, Zeynebe; Goren, Ahmet C.; Alwasel, Saleh H.; Gulcin, Ilhami
Studies have shown an inverse correlation between age-related illnesses like coronary heart disease and cancer and fruit and vegetable intake. Given the probable health benefits of natural antioxidants from plants, research on them has increased. Verbascum L is a large genus of Scrophulariaceae family and 323 species distributed worldwide. Since Verbascum L. species are plants that are grown in many regions of Turkiye and are used in folk medicine, it is important to evaluate the biological activity of these species. In this study antioxidant properties of Verbascum speciosum Schrad. were investigated. The antioxidant capacities of water and ethanol-based extracts obtained from air parts were evaluated with Fe3+ reducing, CUPRAC, FRAP, DPPH center dot, and ABTS(center dot+) scavenging antioxidant methods. Extracts were also examined to determine their AChE, alpha-glycosidase and alpha-amylase enzyme inhibitions. This investigation could be a basis for further phytochemical investigations of Verbascum speciosum Schrad.
Anticholinergic, Antidiabetic and Antioxidant Activities of Ferula Orientalis L. Determination of Its Polyphenol Contents by Lc-Hrms
(Acg Publications, 2021) Kiziltas, Hatice; Goren, Ahmet C.; Bingol, Zeynebe; Alwasel, Saleh H.; Gulcin, Ilhami
To evaluate the antioxidant activity of evaporated ethanolic extract of Ferula orientalis L. (EEFO) and lyophilized water extract of Ferula orientalis L. (WEFO) several in vitro antioxidant methods such as ABTS(center dot+) scavenging activity, DPPH. scavenging activity, Fe(3+)reduction method, cupric ions (Cu2+) reduction capacity, and metal ion (Fe2+)-binding activities using ferrozine reagent were separately performed. Also, BHT, alpha-tocopherol and ascorbic acid were used as the standard antioxidant molecules. Moreover, some phenolic compounds that are responsible for antioxidant abilities of EEFO and WEFO were determined by LC-HRMS. EEFO and WEFO demonstrated effective antioxidant abilities when compared with the standards. EEFO demonstrated IC50 values of 1.946 mu g/mL against acetylcholinesterase (AChE), 0.815 mu g/mL against alpha-glycosidase, and 0.675 mu g/mL against alpha-amylase.
Antidiabetic, Anticholinergic and Antioxidant Activities of Aerial Parts of Shaggy Bindweed (Convulvulus Betonicifolia Miller Subsp.) - Profiling of Phenolic Compounds by Lc-Hrms
(Elsevier Sci Ltd, 2021) Bingol, Zeynebe; Kiziltas, Hatice; Goren, Ahmet C.; Kose, Leyla Polat; Topal, Meryem; Durmaz, Lokman; Gulcin, Ilhami
In order to evaluate the antioxidant activity of evaporated ethanolic extract (EESB) and lyophilized water extract (WESB) of Shaggy bindweed (Convulvulus betonicifolia Mill. Subs), some putative antioxidant methods such as DPPH. scavenging activity, ABTS(center dot)(-) scavenging effect, ferric ions (Fe3+) reduction method, cupric ions (Cu2+) reducing capacity, and ferrous ions (Fe2+) binding activities were separately performed. Also, ascorbic acid, alpha-tocopherol and BHT were used as the standard compounds. Additionally, some phenolic compounds that responsible for antioxidant abilities of EESB and WESB were screened by liquid chromatography-high resolution mass spectrometry (LC-HRMS). At the same concentration, EESB and WESB demonstrated effective antioxidant abilities when compared to standards. In addition, EESB demonstrated IC50 values of 1.946 mu g/mL against acetylcholinesterase (AChE), 0.815 mu g/mL against alpha-glycosidase and 0.675 mu g/mL against alpha-amylase enzymes.
Chemical Profiling by Lchrms, Antioxidant Potential, Enzyme Inhibition, Molecular Docking and Molecular Dynamics Simulations of Acantholimon Acerosum
(Elsevier, 2025) Kiziltas, Hatice; Ortaakarsu, Ahmet Bugra; Bingol, Zeynebe; Erturk, Adem; Goren, Ahmet Ceyhan; Pinar, Suleyman Mesut; Gulcin, Hami
The principal objective of this study is to evaluate phenolic content and biological activity in vitro and silico properties of A. acerosum (Willd.) Boiss. subsp. acerosum's (A. acerosum). The antioxidant activity of A. acerosum was assessed in a variety of bioanalytical methods. Strong antioxidant activity was shown for both water (WEAA) and ethanol (EEAA) extracts of the aerial parts of A. Acerosum. Also, it was shown that hyperoside is the most prevalent phenolic compound in EEAA (12,940.83 mg kg(-1)) and WEAA (5409.67 mg kg(-1)) by LCHRMS. The enzyme inhibitory abilities of the EEAA were then realised. The IC50 values of EEAA against acetylcholinesterase (AChE) (E.C.3.1.1.7), alpha-glycosidase (E.C.3.2.1.20), and alpha-amylase (E.C.3.2.1.1) were determined as 1.828 mu g mL(-1), 0.615 mu g mL(-1), and 1.081 mu g mL(-1), respectively. Subsequently, the alpha-amylase, alpha-glycosidase, and AChE enzymes were subjected to molecular docking and molecular dynamic interactions using major compounds of EEAA as ligands. High enzyme-inhibiting properties of EEAA were also determined in silico. It has been reported that numerous traditional and contemporary medical applications of A. acerosum. It can be said that the effective antioxidant results obtained in our study are the basis of previously performed traditional or contemporary medical practices.
Comprehensive Metabolic Profiling of Acantholimon Caryophyllaceum Using Lc-Hrms and Evaluation of Antioxidant Activities, Enzyme Inhibition Properties and Molecular Docking Studies
(Elsevier, 2022) Kiziltas, Hatice; Bingol, Zeynebe; Goren, Ahmet Ceyhan; Pinare, Sueuroleyman Mesut; Ortaakarsu, Ahmet Bugra; Alwasel, Saleh H.; Gulcin, Lhami
The Acantholimon taxon consists of thorny, perennial and subshrub flowering plants. Extraction of various Acantholimon species are traditionally applied to treat liver disease and diabetes and roots are boiled used to treat wounds and skin irritation. Here, we quantified, the radical scavenging activities of ethanol (EEAC) and water (WEAC) extracts of Acantholimon caryophyllaceum Boiss (A. caryophyllaceum) against 1,1-diphenyl-2-picryl-hydrazyl (DPPHGreek ano teleia) and 2,2-azino-bis3-ethylbenzthiazoline-6-sulfonic acid (ABTS*+) as well as their abil-ity to reduce Fe3+, Fe3+-TPTZ, and Cu2+. The antioxidant activities of both extracts were similar to those of standards in all assays. For instance, IC50 of EEAC and WEAC for scavenging DPPH were 19.8 and 69.3 mg/mL, respectively. We also assessed the inhibitory effects of EEAC and WEAC against acetylcholinesterase (AChE), a-amylase and a-glycosidase, which are associated with Alzheimer's disease and diabetes. The ethanol extract inhibited these enzymes with IC50 of 1.137, 0.172 and 0.511 mg/mL, respectively. The results of liquid chromatography-high resolution mass spectrometry (LC-HRMS) revealed that (-)-epigallocatechin was the main phenolic compound in both EEAC and WEAC. Molecular docking analysis revealed binding interactions between the main phenolic compounds (-)-epigallocatechin gallate, (-)-epigallocatechin, fumaric acid, hyperoside, and myricetin with AChE, a-amylase and a-glycosidase enzymes.(c) 2022 Published by Elsevier B.V. on behalf of SAAB.
Lc-Hrms Profiling and Antidiabetic, Anticholinergic, and Antioxidant Activities of Aerial Parts of Kinkor (Ferulago Stellata)
(Mdpi, 2021) Kiziltas, Hatice; Bingol, Zeynebe; Goren, Ahmet Ceyhan; Kose, Leyla Polat; Durmaz, Lokman; Topal, Fevzi; Gulcin, Ilhami
Kinkor (Ferulago stellata) is Turkish medicinal plant species and used in folk medicine against some diseases. As far as we know, the data are not available on the biological activities and chemical composition of this medicinal plant. In this study, the phytochemical composition; some metabolic enzyme inhibition; and antidiabetic, anticholinergic, and antioxidant activities of this plant were assessed. In order to evaluate the antioxidant activity of evaporated ethanolic extract (EEFS) and lyophilized water extract (WEFS) of kinkor (Ferulago stellata), some putative antioxidant methods such as DPPHGreek ano teleia scavenging activity, ABTS(center dot+) scavenging activity, ferric ions (Fe3+) reduction method, cupric ions (Cu2+) reducing capacity, and ferrous ions (Fe2+)-binding activities were separately performed. Furthermore, ascorbic acid, BHT, and alpha-tocopherol were used as the standard compounds. Additionally, the main phenolic compounds that are responsible for antioxidant abilities of ethanol and water extracts of kinkor (Ferulago stellata) were determined by liquid chromatography-high-resolution mass spectrometry (LC-HRMS). Ethanol and water extracts of kinkor (Ferulago stellata) demonstrated effective antioxidant abilities when compared to standards. Moreover, ethanol extract of kinkor (Ferulago stellata) demonstrated IC50 values of 1.772 mu g/mL against acetylcholinesterase (AChE), 33.56 +/- 2.96 mu g/mL against alpha-glycosidase, and 0.639 mu g/mL against alpha-amylase enzyme respectively.
Lc-Hrms Profiling of Phytochemicals, Antidiabetic, Anticholinergic and Antioxidant Activities of Evaporated Ethanol Extract of Astragalus Brachycalyx Fischer
(Acg Publications, 2021) Kiziltas, Hatice; Bingol, Zeynebe; Goren, Ahmet C.; Pinar, Suleyman Mesut; Alwasel, Saleh H.; Gulcin, Ilhami
Astragalus is a perennial plant that has existed for about 2500-3000 years and consists of more than 250 taxonomic parts. Twenty species of Astragalus are endemic to Turkey, as well as the richest genus with 425 taxa. The roots of Asfragalus species are used in folk medicine as hepatoprotective, antioxidant, antibacterial, antihypertensive, antidiabetic and diuretic. Also, it is used to treat diabetes mellitus, leukemia, nephritis and uterine cancer. It is known that in Anatolia, Astragalus roots are traditionally used against leukemia and wound healing. For the purpose, the measuring of antioxidant activity of evaporated ethanol extract of Asfragalus brachycalyx FISCHER (EEAB), some bioanalytic methods including DPPH center dot and ABTS(center dot+) scavenging effects, ferric ions (Fe3+) and cupric ions (Cu2+) reducing abilities, and metal (Fe') chelating activity were realized. alpha-Tocopherol, ascorbic acid, and BHT were used as the standard antioxidants. On the other hand, some phenolic compounds, which responsible for antioxidant activities of EEAB was determined by liquid chromatography-high resolution mass spectrometry (LC-HRMS). At the similar concentration, EEAB exhibited efficient antioxidant effects when compared to standard compounds. Additionally, EEAB showed IC50 values of 1.985 mu g/mL toward acetylcholinesterase (AChE), 0.620 mu g/mL on alpha-glycosidase and 0.306 mu g/mL against alpha-amylase enzymes.
Sage (Salvia Macrochlamys): Lc-Hrms for Phytochemical Analysis, Cytotoxicity, Enzyme Inhibition, Antioxidant Activity, Molecular Docking and Molecular Dynamics Simulations
(Taylor & Francis Ltd, 2024) Kiziltas, Hatice; Ortaakarsu, Ahmet Bugra; Bingol, Zeynebe; Goren, Ahmet Ceyhan; Pinar, Suleyman Mesut; Gulcin, Ilhami
This study primarily aims to evaluate the phenolic content and biological activity of Salvia macrochlamys both in vitro and in silico. The antioxidant activities of Salvia macrochlamys were assessed using various methods. Additionally, cytotoxic effects and the alpha-amylase, alpha-glucosidase, and acetylcholinesterase (AChE) enzyme inhibitory abilities of ethanolic extract of the aerial parts of Salvia macrochlamys (EESM) were evaluated. Then, the molecular docking interactions and molecular dynamic calculations of EESM against alpha-amylase, alpha-glycosidase, and AChE enzymes were carried out. High antioxidant activity was determined in both ethanol and water extracts. The IC50 values of EESM against AChE (EC 3.1.1.7), alpha-glycosidase (E.C.3.2.1.20), and alpha-amylase (E.C.3.2.1.1) were determined as 1.622 mu g/mL, 0.530 mu g/mL, and 1.320 mu g/mL, respectively. High enzyme-inhibiting properties of EESM were determined in also in silico. Rosmarinic acid was identified as one of the most abundant phenolic compounds in both EESM and water extract of the aerial parts of Salvia macrochlamys (WESM) (3919.65 mg/kg and 10520.77 mg/kg, respectively). Their high level of flavonoids and phenolics, as well as the potent antioxidant and enzyme-inhibitory properties displayed by extracts, may contribute to broadening the use of Salvia macrochlamys in both traditional and modern therapeutic applications.