Browsing by Author "Bulduk, M."

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The Effect of Resveratrol Therapy on The Vascular Responses Caused by Chronic Fluorosis
(int Soc Fluoride Research, 2020) Bulduk, M.; Oto, G.; Ozdemir, H.; Demirel-Yilmaz, E.
The aim of this study was to investigate the effect of resveratrol therapy on the contraction-relaxation responses of the thoracic aorta rings and on the blood pressure of rats exposed to chronic fluorosis. The study was conducted using 80 male and female Sprague Dawley rats weighing 200-250 g. The rats were randomly divided into 8 same gender groups of 10 (4 groups of male rats and 4 of female rats) and administered, for each gender via the drinking water, (i) 0 mg/L of sodium fluoride (NaF) and 0 mg/L of resveratrol (control groups); (ii) 10 mg/L of NaF; (iii) 50 mg/L of resveratrol; and (iv) 10 mg/L of NaF and 50 mg/L of resveratrol. Blood pressures were measured on days 30, 60, and 90 in each group. At the end of day 90, the contraction-relaxation responses of the thoracic aorta rings were examined in an isolated organ bath. The analysis on the serum fluorine level revealed that the serum fluorine level was elevated in all the rats administered NaF. For each gender, the most marked elevations in the blood pressures were seen, on all three days, in the NaF groups. In both the male and female groups, the chronic administration of resveratrol with NaF led to decreased blood pressures. The contraction response resulting from phenylephrine administration was increased in the groups administered NaF, whereas it was decreased in the groups administered NaF+resveratrol. In the groups administered resveratrol, the maximal relaxing effect was achieved by acetylcholine. In the groups administered NaF, the relaxation responses caused by SNP (sodium nitroprusside) were similar to those in the other study groups. The results of this study indicate that resveratrol provides a protective effect against the increased blood pressure caused by NaF and the potential endothelial damage. The protective effect of resveratrol results from its capability to reduce fluorine-induced oxidative stress and endothelial tissue damage. Further studies are warranted to investigate the adverse effects in the veins exposed to chronic fluorosis and the molecular mechanisms of the potential protective effects of resveratrol against these adverse effects.
The Protective Role of Resveratrol on Serum Total Sialic Acid and Lipid-Bound Sialic Acid in Female Rats With Chronic Fluorosis
(Yuzuncu Yil Universitesi Tip Fakultesi, 2016) Oto, G.; Ekin, S.; Özdemir, H.; Bulduk, M.; Uyar, H.; Öksüz, E.
In the present study, the effect of resveratrol on serum total sialic acid (TSA) and lipid bound sialic acid (LSA) was investigated in the rats exposed to chronic fluoride. The study was administered using 32 female Sprague Dawley rats weighing 200-250 g. Rats were divided into four groups (n=8/group). Group I comprised the control group, group II was treated with sodium fluoride (NaF) (10 mg/L/day), group III was treated with resveratrol (50 mg/L/day) and group IV was treated with NaF+resveratrol for 90 days period. Total sialic acid (TSA) and lipid-bound sialic acid (LSA) were determined in serum samples. Statistical analysis showed that the NaF group was significantly higher than the control group with regards to LSA (17.59±2.734 mg/dL, 12.61±2.013 mg/dL) and TSA (87.86± 8.34 mg/dL, 71.47± 8.57 mg/dL) levels (p<0.01 and p<0.05 respectively). Whereas the Resveratrol group was also significantly lower than the NaF group regarding LSA (13.21±2.848 mg/dL, 17.59±2.734 mg/dL) and TSA (72.44± 10.43 mg/dL, 87.86± 8.34 mg/dL) levels (p<0.05 and p<0.05 respectively), Moreover, no significant differences in LSA (14.62±1.85 mg/dL, 12.61±2.013 mg/dL) and TSA (81.19 ±10.24 mg/dL, 71.47± 8.57 mg/dL) levels were observed in the Resveratrol + NaF groups, as compared to the control group (p>0.05). The present study demonstrated slight positive and beneficial effect of resveratrol on the concentration levels of LSA and TSA in serum. © 2017 Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.