Browsing by Author "Burak, A.K."

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Color Distribution of Gingiva Created by Color Schema Maker Software
(Yuzuncu Yil Universitesi Tip Fakultesi, 2016) Burak, A.K.; Kaki, B.
The aim of this study is to create a true gingival shade guide using schema maker softwares. 128 patients’ gingival colors were recorded by a standardized photograph machine under standard light and distance conditions. The recorded images were analyzed by Gimp software to extract RGB values by using color pick tool. RGB values were converted to CIE L*a*b* values. L* values were clustered by ward method. The average CIE L*a*b* values calculated. Gingival images were layered by Gimp software and evaluated by Wenovo pictures to colors software v1.0. According to this study, 3 clusters using CIE L values and 9 subclusters with using CIE a*b* values have calculated by hierarchical and k means clustering methods. 256 colors that can be used for gingiva color determination has been calculated by Wenovo color schema maker software. With the limits of this study, colors of the gingiva were evaluated for only white race and patients who admitted to our university. Wenovo schema maker software calculated more colors than statistical method that can be used for color determination of the gingiva. Further studies are needed to create more reliable color gingiva shade guide with a large number of patients and different races and places. © 2016, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
The Use of Methylene Blue as Mouthwash in Periodontology
(Yuzuncu Yil Universitesi Tip Fakultesi, 2015) Burak, A.K.; Özeroğlu, E.; Taspinar, M.
The aim of this study is to investigate the cytotoxicity effects of methylene blue on the human gingival fibroblasts cell-lines in vitro. 3T3 human gingival fibroblast cell-lines were used to evaluate the cytotoxicity of mouthwashes containing methylene blue and chlorhexidine gluconate. The cultured fibroblasts were divided into two groups which subjected into chlorhexidine gluconate or methylene blue in vitro. The cells viability was determined at 30 seconds, 1 minute and 3 minutes of exposure to mouthwashes using by XTT colorimetric assay. Spectrophotometric absorbance was measured at 550 nm using ELISA analyzer. The IC50 values were calculated for each time points for methylene blue and chlorhexidine gluconate mouthwashes. The effect of the methylene blue and chlorhexidine gluconate on the human gingival fibroblast viability was expressed as a percentage of the control groups. Comparison between two groups in different time and concentration values showed that, chlorhexidine gluconate were found to be more cytotoxic on gingival fibroblast than methylene blue. Cell viability exposured to methylene blue in 1% concentration during 3 minutes was 99% and in 100% concentration the cell viability was 88%. The chlorhexidine gluconate at the same conditions was 92% and 18% respectively. Our study demonstrates that 100% chlorhexidine gluconate (0.2% chlorhexidine gluconate concentration in commercially available products) has much more cytotoxic effect than methylene blue to human gingival fibroblast at clinical use time and different concentrations in vitro. © 2015, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.