Browsing by Author "Cavusoglu, Mehmet"

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Detailed Chemical Investigation of the Essential Oil and Aroma Contents of 21 Hypericum Triquetrifolium Turra Species Collected From Different Localities via a Chemometric Approach
(John Wiley and Sons Ltd, 2026) Yigitkan, Serkan; Yigitkan S.; Akdeniz, Mehmet; Olmez, Ozge Toku; Cavusoglu, Mehmet; Yener, Ismail; Firat, Mehmet
Hypericum species have been used for medicinal purposes since ancient times because of their important pharmacological properties. Hypericum triquetrifolium Turra belongs to the Hypericaceae family and is native to Eastern Europe and the Mediterranean region. In this study, the essential oil and aroma contents of H. triquetrifolium collected from 21 different localities in the eastern region of Turkey were determined via GC–MS/FID. In addition, the antioxidant and enzyme inhibition activities (cholinesterase, tyrosinase and urease) of the essential oils and major components were determined. Finally, the essential oil and aroma contents were evaluated chemically. The major components of H. triquetrifolium collected from 21 different locations were generally determined to be octane, 2-methyl (1.35%–25.18%), α-pinene (1.32%–19.1%), nonane, 3-methyl (2.43%–24.59%) and caryophyllene (3.79%–30.78%). When the aroma content was examined, octane, 2-methyl (14.22%–44%), nonane (6.37%–18.29%), nonane, 3-methyl (7.62–23.12%) and decane, 2-methyl (3.46%–22.74%) were generally identified as the major components. Overall, all samples were found to have moderate antioxidant capacity. In the AChE enzyme inhibition test, at a concentration of 100 μg/mL, samples S-19, S-9 and S-11 (inhibition %: 87.61 ± 0.63; 84.29 ± 1.51; 82.28 ± 0.53, respectively), and in the BChE enzyme inhibition test, the S-1, S-4 and S-6 samples (88.76 ± 0.79; 91.29 ± 1.58; 93.15 ± 2.16, respectively) were found to be more active than the reference galantamine. In the tyrosinase enzyme inhibition test, samples S-5 and S-13 (inhibition %: 57.09 ± 2.53 and 66.86 ± 0.50, respectively) were found to have greater activity than the other samples. A total of 21 H. triquetrifolium samples collected from different regions of Diyarbakır, Mardin, Elazığ, Siirt, Batman and Şanlıurfa Provinces were analysed via principal component analysis (PCA) and cluster analysis (CA) techniques on the basis of volatile and aroma components. PCA revealed that the dominant volatile components in the samples formed three groups, and regional differences had no significant effect on the samples. The first two principal components explained 59.2% of the variance. The antioxidant capacities of essential oils, their high contents of compounds such as α-pinene and caryophyllene, and their strong acetyl and butyrylcholinesterase enzyme inhibition activities are considered to have potential for use in the food, cosmetics and pharmaceutical industries. © 2026 John Wiley & Sons Ltd.
Lc-ms/Ms Analysis and Biological Activities of Different Parts of Ziziphora Capitata L
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2024) Yigitkan, Serkan; Cavusoglu, Mehmet; Caglayan, Mehmet Veysi; Yener, Ismail; Firat, Mehmet; Cavus Kaya, Eda; Ertas, Abdulselam
The Ziziphora species, classified under the Lamiaceae family, have a strong aromatic property. Ziziphora species have been used in folk medicine as sedative, gastric, aphrodisiac, bloating, and degassing. In the current study, the phenolic and flavanoid content of ethanol extracts of Ziziphora capitata L. species of flower, leaf, branch, mixed, and root parts was determined by the LC-MS/MS device. In addition, the antioxidant and cytotoxic activities of the extracts, as well as their inhibitory effects on enzymes (antihypertensive, AchE (acetylcholinesterase), BchE ( butyrylcholinesterase), elastase, tyrosinase, collagenase and urease), were determined. The LC-MS/MS results showed that quinic acid (25578, 5842, 25171, 14055, 10597 mu g g-1, respectively) was found in higher amounts in flower, leaf, branch, mixed, and root extracts of Z. capitata species compared to other components. Additionally, rosmarinic acid (17097 mu g g-1), cynaroside (8432), and hesperidin (8067) were found to be major components. It was observed that the flower extract of the species exhibited strong antioxidant activity (IC50: 37.18 +/- 1.36 mu g mL-1, 9.89 +/- 0.45, A0.5:16.27 +/- 0.02, respectively) in DPPH, ABTS and CUPRAC methods. It was concluded that the leaf extract of Z. capitata species had a strong cytotoxic effect on HT-29 (colon cancer cell line) (viability %: 9.26 +/- 0.69). It was observed that the root part of the species exhibited higher activity in butyrylcholinesterase (BChE) enzyme inhibition activity (inhibition %: 40.56 +/- 0.88) than other parts. It was determined that Z. capitata extracts did not show acetylcholinesterase, urease, tyrosinase, elastase, collagenase, and antihypertensive enzyme activity or showed low activity. As a result, it is thought that the flower extract of the Z. capitata species has better results in terms of the examined parameters, whereas the leaf extract needs to be subjected to more detailed in vitro and in vivo research conducted to be used in the pharmaceutical industry as a result of its cytotoxic effect against colon cancer cell lines.