Browsing by Author "Celik, I"

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Effects of Acute Trichloroacetic Acid Treatment on the In-Vivo Activity of Glucose 6-Phosphate Dehydrogenase and Carbonic Anhydrase in Various Tissues of Rats
(Parlar Scientific Publications (p S P), 2005) Celik, I; Demir, H
This study was carried out to investigate possible trichloroacetic acid (TCA) effects on the activity of glucose 6-phosphate dehydrogenase (G6PD) and carbonic anhydrase (CA) in muscle and liver tissues of rats under laboratory conditions. Five Sprague-Dawley albino rats were used for experiments, and 200 mg TCA/kg body weight was administered intraperitoneally. Tissue G6PD and CA activities in muscle and liver of rats were determined six hours after treatment. According to the results, the TCA treatment caused significant decrease in muscle and liver G6PD, but no significant difference in liver CA level was observed, in comparison to control rats. Therefore, it is conceivable that TCA is a toxicant like other pesticides, and is primarily interacting with liver and muscle tissue cells.
Effects of Fresh Butter Consumption on the Lipid Profile in Healthy Human Male
(Journal Clinical Biochemistry & Nutrition, 2005) Gorguc, M; Celik, I
The present study was carried out to investigate whether fresh butter could affect on the serum lipid profile, total triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-c), high density lipoprotein cholesterol (HDL-c), %LDL-c and HDL-c/LDL-c ratio in human. The research was conducted on fifteen volunteer healthy human males, whose background, clinical and dietary information were collected. Fresh butter 20 g/day was taken by individuals at breakfast for 20 days without changing their traditional diet. Blood samples were taken at 10th and 20th days from each individual. According to experimental data, fresh butter consumption was found to cause different effects on the levels of serum TG, TC, LDL-c, HDL-c,%LDL-c and HDL-c/LDL-c in comparing with the beginning values. Their values did not significantly increase at 10th day. However, the levels of TG and HDL-c increased significantly at 20th day of butter consumption period. Also, there was an appreciable increase in TC, LDL-c and %LDL-c levels causing arteriosclerosis at both 10th and 20th day of research period. It was concluded that fresh butter might affect on human lipid profile.
Effects of Indole-3 Acid and Kinetin on the Adenosine Deaminase Activity in Various Tissues of Rats
(Asian Journal of Chemistry, 2006) Tuluce, Y; Celik, I
The effects of a sub-lethal concentration of some plant growth regulators on heart, kidney and muscle adenosine deaminase activity in female rats (Sprague-Dawley) were investigated under laboratory conditions. Thirty-six Sprague-Dawley albino rats were divided into three experimental groups: control and plant growth regulator treatment [indole-3-acetic acid and kinetin]. 100 ppm each of plant growth regulators, indole-3-acetic acid and kinetin were administered orally to 12 rats ad libitum for 25 d continuously. The hormone treatment caused different effects on the heart adenosine deaminase activity in comparison to those of the control rats. According to results, while the heart adenosine deaminase activity was decreased significantly by indole-3-acetic acid and kinetin, these plant growth regulators did not affect the adenosine deaminase activity of kidney and muscle tissues. In conclusion, these chemicals have toxicological effects on the animals in subacute treatment.
Effects of Indoleacetic Acid and Kinetin on Lipid Peroxidation and Antioxidant Defense in Various Tissues of Rats
(Academic Press inc Elsevier Science, 2006) Celik, I; Tuluce, Y
This study aims to investigate the effects of indoleacetic acid (IAA) and kinetin (Kill, which are plant growth regulators (PGRs), oil antioxidant defense systems [reduced glutathione (GSH), glutathione-S-transferase (GST), catalase (CAT)], and lipid peroxidation level (malondialdehyde, MDA) various tissues of rats. Rats (Sprague-Dawley albino) were exposed to 100 ppm IAA and Kn. One hundred parts per million of PGRs was administered orally to rats ad libitum for 21 days continuously. The PGRs treatments caused different effects oil the content of MDA and antioxidant defense system in comparison to those of control rats. According to the results, the subchronic treatments of IAA caused significant decrease in the GSH concentration and CAT activity in erythrocyte. Kn decreased GSH concentration in erythrocyte too. While the MDA concentration in brain was increased significantly by IAA and Kn, Kn decreased significantly brain CAT and GST activity. The liver GST activity was decreased by IAA and Kn. But, liver CAT activity was increased by IAA. On the other hand, while IAA treatment caused a significant decrease kidney GST activity, Kn caused a significant decrease both kidney GST and CAT activity. Also, while heart CAT activity was decreased by IAA, heart GST activity was decreased by both IAA and Kn. Moreover, MDA concentration in heart was increased by Kn treatment. It was concluded that IAA might effect MDA and antioxidant defense on the animals at subchronic treatment. (c) 2005 Elsevier Inc. All rights reserved.
The Effects of Some Fungicides on Activity of Eight Serum Enzymes in Vitro
(Marcel dekker inc, 1997) Kara, M; Celik, I
The purpose of the present study was to investigate the effects of Benlate [1-(N-butyl carbomyl), 2-(metoxy carboxyamido)-benzimidazol], Penncozep [mangan-zinc ethylenediamine- bisdithicarbamate], Bayleton [1-(4- chlorophenoxy)- 3,3 -dimethyl-1(1-H-1,2,4 - thiazol -1- yl)- 2.2 - butanon], Cupravit [Cuper oxichloride] and Dithane [Manganese etylenebidithiocarbamate] on human serum enzymes, myocardial creatine kinase (CK-MB), amylase, creatine kinase (CK), aspartate amino transferase (AST), serum glutamyl pruvic transferase(SGPT), alkaline phosphatase (ALK-P), delta glutamyl transferase (GGT-P) and lactate dehydrogenase (LDH), in vitro. Bayleton inhibited only SGPT and it was ineffective on the other seven enzymes. Benlate, Penncozep, Cupravit and Dithane inhibited some enzymes, but activated the others. Benlate as the strongest inhibitor for CK-MB, Cupravit for Amylase, Dithane for ALK-P, Penncozep for CK, AST, SOFT and GGT-P. No inhibition was occurred in LDH. Of the fungicides studied the maximum effective one was Penncozep, the minimum effective one was Bayleton. The most inhibition was shown in SGPT and CK. Cupravit was found as an activator rather than an inhibitor.
The Effects of Some Pesticites on the Activity of Liver and Erythrocyte Enzymes (In Vitro)
(Taylor & Francis inc, 1996) Celik, I; Camas, H; Arslan, O; Yegin, E; Kufrevioglu, OI
The effect of pomarsol (tetramethyltriurem disulphide), Benlate 1-(N-Butlycarbomoyl) 2-(Metoxycarboxamido)-bezimidazol, meothrin (alpha-cyanophenoxbenzyl 2.2.3.3.-tetramethyl cyclopropanecarbonylate), Imperator [alpha-cyano 3-phenoxybenzyl-cis, trans-3- (2.2-dichroinyl)-2.2- dimetil research on GOT, GPT (liver enzymes) and CA-I, CA-II (Human erythrocyt) BCA (Bovine erythrocyt). Among the chemicals meothrin, imperator, sumicidin were determined to have inhibitory effect, on bovine CA, human CA and GOT enzymes, but ineffective on GPT enzyme. The I-50 values of chemicals caused inhibition were determined by means of activity percentage [I] diagrams. The values were 3.2x10(-2) M, 3.5x10(-2) M, 2.7x10(-2) M for GOT respectively. The values of same chemicals were 5.8x10(-3) M, 1.00x10(-2) M, 1.22x10(-2) M for CA-I, and 7.11x10(-3) M, 2.68x10(-2) M, 1.22x10(-2) M for CA-II respectively on the order nand, these chemical were 9.25x10(-3) M, 8.65x10(-3) M and 1.63x10(-2) M for bovine CA.
The Effects of Some Plant Growth Regulators on the Level of Glycogen Protein and Lipid in Various Tissues of Rats
(Parlar Scientific Publications (p S P), 2006) Ozok, N; Celik, I
The study was carried out to investigate whether plant growth regulators (PGRs) could affect carbohydrate, protein and lipid metabolism in liver and muscle tissues. Forty-eight Sprague-Dawley albino rats were divided into four experimental groups; control and PGR-treated [indole acetic acid (IAA), indole butyric acid (IBA) and kinetin (Kn)]. 100 ppm of PGRs was administered orally to 12 rats ad libitum for 25 days continuously. The PGR treatments caused different effects on the level of protein, glycogen and lipid in comparison to those of control rats. According to the results, while the level of lipid and glycogen in muscle and liver of rats exposed to IAA increased statistically, the protein levels remained unchanged in both muscle and liver in comparing with the controls. IBA treatment caused a statistically important decrease in the levels of protein in liver, and an increase in the glycogen level in muscle. Also, kinetin caused a statistically important decrease in the glycogen level in liver and muscle. The protein and lipid levels remained unchanged in both muscle and liver. It is concluded from this study, that PGRs may cause toxicity in different tissues of rats.
Effects of Subchronic Exposure To Thiocarbamide on Haematological and Biochemical Constituents of Rats
(Parlar Scientific Publications (p S P), 2004) Celik, I; Ozbek, H
The effects of sublethal concentration of thiocarbamide on various haematological and biochemical constituents of rat were investigated under laboratory conditions. 250 ppm of thiocarbamide was orally administered to eight ad libitum-fed rats during the test period of 25 days. Various haematological and biochemical constituents of the rat were determined after treatment with thiocarbamide, which caused significant increases in lactate dehydrogenase (LDH) and creatine phosphokinase (CPK), while the level of alanine aminotransferase (ALT) was decreased. Aspartate aminotransferase (AST) and amylase level did not change. On the other hand, the treatment of rats with thiocarbamide also resulted in a different effect on the level of blood constituents compared to that of control rats. While the levels of white blood corpuscles (WBC) and thrombocytes (PLT) were increased significantly by thiocarbamide, the other parameters did not change. With regard to the biochemical characteristics, the levels of total cholesterol (TC) and high density lipoprotein cholesterol (HDLC) were increased significantly by thiocarbamide, but the other consituents did not change. It is concluded from the experiments of this study that thiocarbamide may cause toxicity in different tissues/organs of the laboratory in rats.
Efficacy of Polyvinylpolypyrrolidone in Reducing the Immunotoxicity of Aflatoxin in Growing Broilers
(Taylor & Francis Ltd, 2000) Celik, I; Oguz, H; Demet, Ö; Dönmez, HH; Boydak, M; Sur, E
1. Protective action of an enzyme-linked polyvinylpolypyrrolidone (PVPP, Mycofix(R) Plus) against the immunosuppressive effect of afatoxins (AF) was evaluated by determination of peripheral blood TP-lymphocyte proportions and splenic plasma cell counts. Histological changes in lymphoid organs were also investigated by light microscopy. One-d-old broiler chicks (Hybro) received 2.5 mg/kg diet AF (83.06% AFB(1), 12.98% AFB(2), 2.84% AFG(1), 1.12% AFG(2)) with or without PVPP (3 g/kg diet) until 21 d of age. 2. When compared with the controls, AF treatment significantly decreased peripheral T-lymphocyte counts. AF caused a slight decrease in splenic plasma cell counts. The addition of PVPP to an AF-containing diet significantly increased T-lymphocyte counts. Splenic plasma cell counts were numerically intermediate between control and AF groups. 3. The dietary addition of PVPP to AF-free diet did not significantly alter either T-lymphocyte or splenic plasma cell counts.
Embryotoxicity Assay of Aflatoxin Produced by Aspergillus Parasiticus Nrrl 2999
(Taylor & Francis Ltd, 2000) Celik, I; Oguz, H; Demet, Ö; Boydak, M; Dönmez, HH; Sur, E; Nizamlioglu, F
1. The embryotoxicity of mixed aflatoxins (AF) and aflatoxin B-1 (AFB(1)) were evaluated by a modified chick embryotoxicity screening test (CHEST). Adverse effects on the early embryonic development of thymus and bursa of Fabricius were also investigated by light microscopy. AF consisted of 83.06% AFB(1), 12.98% AFB(2), 2.84% AFG(1) and 1.12% AFG(2). 2. A total of 448 fertilised laying hens' eggs were used. AF and AFB(1) were injected into the eggs at doses of 10, 100 and 1000 ng/egg. Embryonic developmental stages were evaluated according to the Hamburger-Hamilton scale (HH-scale). 3. The results showed that AFB(1) given at 10 ng/egg had a significantly (P<0.05) greater embryotoxic effect than AF given at a similar dose. The higher doses of both AF and AFB(1) caused higher embryonic mortality and also an increase in early deaths. 4. In the groups receiving 100 ng/egg AF and AFB(1) an abnormal development was seen, with a protruded central region, corresponding to the area pellucida of the blastoderm. No other developmental abnormality attributable to AF or AFB(1) was found.
Influence of Some Pesticides on Activity of Seven Serum Enzymes (In Vitro)
(Marcel dekker inc, 1997) Sekeroglu, MR; Celik, I; Arslan, O
The purpose of this study was to investigate the effects of Endosulfan [6,7,8,9, 10, 10-hexachloro- 1,5. 5a. 6, 9, 9 a hexahydro- 6, 5, methano - 2, 4, 3-benzo (e) dioxathpiepin 3- oxide], Mavrik [alpha-cyano -3-phenoxybenzyl M -(2 chloro-alpha-alpha-alpha-triflouro-p-tolyl)- D - valinate], 2,4-D (2,4-Dichlorophenoxyacetic acid) and Neoran (isopropyl -4,4-dibromobenzilate) on serum amylase (EC 3.2.1.1), creatine kinase (CK; EC 2.7.3.2) aspartate amino transferase (AST; EC 2.6.1.1), alanine aminotransferase (ALT; EC 2..6.1.2), alkaline phosfatase (ALP; EC 3.1.3.1), gamma glutamyl transferase (GGT; EC 2.3.2.2) and lactate dehydrogenase (LDH; EC 1.1.1.27). I-50 values of chemicals caused inhibition were determined by means of activity percentage -[I] diagrams. Endosulfan had effect of inhibition only on AST. The I-50 value of chemical was determined as 3.13x10(-4) M. Mavrik had effects of inhibition on AST, ALT, ALP and LDH. The values of I-50 were 7.83x10(-4) M, 1.17x10(-4), 1.23x10(-3) M and 2.23x10(-3) M, respectively. 2,4-D had effects of inhibition on ALT, ALP, GGT and LDH. The I-50 values of chemical were 6.97x10(-2) M, 5.05x10(-2) M, 2.35x10(-2) M, 1.07x10(-2) M, respectively. On the other hand, Neoran had effects of inhibition of all enzymes except amylase. I-50 values of this compound on other enzymes (CK, AST, ALT, ALP, GGT and LDH) were 4.41x10(-4) M, 5.06x10(-3) M, 2.40x10(-3) M, 5.23x10(-3) FA,4.4x10(-3) M, 5.97x10(-3) M, respectively.
A Light Microscopic Study on the Uptake and Transportation Route of Carbon Particles in the Canine Palatine Tonsil
(Ecole Nationale veterinaire Toulouse, 2001) Baykan, M; Celik, I; Gezici, M; Donmez, HH; Eken, E; Sur, E; Ozkan, Y
Uptake of carbon particles through the epithelium and their transportation route in the canine palatine tonsils were investigated by light microscopy. Reticular and non reticular regions were distinguished in both oral surface and crypt epithelium. Particle ingestion was peculiar to the reticular epithelium. The ingested material was first transported mainly in phagocytosed form to the subepitelial connective tissue and following into the deeper regions, to the bases of lymphoid follicles through the interfollicular tissue. Any free or phagocytosed particles were not observed at the germinal centers of the follicles. It was concluded that the tonsils might operate as a primary sampling area for restricted number of antigens which the animal is exposed. On the other hand, they may contribute to the common mucosal immune system via trafficking of both antigen primed lymphocytes and antigen phagocytosed macrophages those were frequently observed in the luminae of venous and lymphatic sinuses, located at the center of the tonsil.